scholarly journals Gamal (Gliricidia Sepium) Leaf Extract Increase Mortality of Fasciola gigantica In Vitro

2021 ◽  
Vol 4 (2) ◽  
pp. 193
Author(s):  
Brillia Zulianti ◽  
Iwan Sahrial Hamid ◽  
Aditya Yudhana ◽  
Jola Rahmahani ◽  
Maya Nurwartanti Yunita ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Leaf Extract ◽  
In Vitro Study ◽  
Gliricidia Sepium ◽  
Fasciola Gigantica ◽  
Economic Losses ◽  
Randomized Design ◽  
The Mean ◽  
Range Test

Fasciolosis is a disease caused by infection with liver fluke (Fasciola sp). Fasciolosis is a parasitic disease that is important because it can cause high economic losses. The aim of this study was to investigate the effect of gamal (Gliricidia sepium) leaf extract against mortality of Fasciola gigantica in vitro study. In present study was completely randomized design with six treatments and each treatment were divided into four replications. A total of 240 specimens from infected cattles were used in this study. The observation and recording of F. gigantica mortality in all treatments with concentrations of gamal leaf extract P1 (1%), P2 (2%), P3 (3), P4 (4%), K + (Nitroxynil 2%) and K- (physiological NaCl) rate was done in 30, 60, 90, 120, 150, 180, 210 and 240 minutes, respectively. The mortality of F. gigantica was confirmed if there was no movement when triggered by anatomy tweezers then dipped in slightly warm water (50 15℃"> ). The data was analyzed using ANAVA Faktorial and continued with Duncan Multiple Range Test. The results of this study were shown the lowest and the highest mortality rate recorded at minutes 30 and 240 respectively. Moreover, the mean of mortality rate were peaked in nitroxynil (K+) and 1% gamal leaf extract treatment, while the lowest mean of mortality rate occurs in NaCl (K-) treatment. Present study conclude that gamal leaf extract has anthelmintic effect on the mortality rate of Fasciola gigantica. Gamal leaf extract with 1% concentration was claimed to be the most effective.

scholarly journals Anthelmintic Activity of Ocimum sanctum Linn. Leaves Ethanol Extract Against Fasciola gigantica in vitro

2017 ◽  
Vol 3 (6) ◽  
pp. 266
Author(s):  
Mesia Margi Mahardika ◽  
Sri Agus Sudjarwo ◽  
Setiawan Koesdarto
Keyword(s):  
Exposure Time ◽  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Probit Analysis ◽  
Fasciola Gigantica ◽  
Ocimum Sanctum ◽  
Randomized Design ◽  
Range Test ◽  
Lc50 And Lc90

The aims of this research are to know the concentration, exposure time and interaction between concentration and exposure time of Ocimum sanctum Linn. leaves ethanol extract which cause the most mortality toward Fasciola gigantica. Also to know its value of LC50 and LC90. The research was completely randomized design. There were five treatments. Each treatment was done in four replications and used 10 Fasciola gigantica. The observation and recording of dead Fasciola gigantica were done at 0, 2, 4, 6, 8 and 10 hours. Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (50ºC). The obtained data was analyzed using ANOVA Factorial and continued with Duncan Multiple Range Test. The result was 10% concentration and exposure time for 10 hours caused the most mortality toward Fasciola gigantica. However, the interaction between concentration and exposure time resulted that 5% concentration for 8 hours already caused the most mortality of Fasciola gigantica. Probit analysis was used to calculate the LC50 and LC90. The results were LC50 of Ocimum sanctum Linn. leaves ethanol extract was 7.9% at 4 hours, 3.7% at 6 hours, 1.8% at 8 hours and 0.8% at 10 hours and the LC90 was 8.4% at 10 hours. Key words: Ocimum sanctum Linn. leaves, Fasciola gigantica, ethanol extract, in vitro.

scholarly journals The Anthelmintics Activity of Ethanol Extract of African Leaf (Vernonia amygdalina) on Mortality of Fasciola gigantica in Vitro

2019 ◽  
Vol 3 (1) ◽  
pp. 15
Author(s):  
Dhio Asmaydo ◽  
Iwan Sahrial Hamid ◽  
Muchammad Yunus ◽  
Kusnoto Kusnoto ◽  
Mohammad Sukmanadi ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Fasciola Gigantica ◽  
Duncan Multiple Range Test ◽  
Vernonia Amygdalina ◽  
In Vitro Method ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Range Test ◽  
Research Show

The Aims of this study is to know the anthelmintics activity of ethanol extract of african leaf (Vernonia amygdalina) on mortality of Fasciola gigantica in Vitro. Method that used in the research was a completely randomized design. There were five treatments and each treatment was done in four replications. This research used ten Fasciola gigantica in each treatment for all replications. The observation and recording of dead Fasciola gigantica was done at 2, 4, 6, 8, and 10 hours.  Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (500C). The obtained data was analyzed using ANOVA and continued with Duncan Multiple Range Test. The result of this research show that ethanol of extract of African Leaf (Vernonia amygdalina) has activity anthelmintic on mortality of Fasciola gigantica in Vitro. Ethanol extract african leaf (Vernonia amygdalina) with concentration of 1.4% is the optimal concentration of anthelmintics. This higher the concentration of the extract, the higher the property of anthelmintics.

scholarly journals Shear bond strength evaluation of bonded molar tubes on fluorotic molars

2012 ◽  
Vol 83 (1) ◽  
pp. 152-157 ◽  
Author(s):  
Erika L. Silva-Benítez ◽  
Veronica Zavala-Alonso ◽  
Gabriel A. Martinez-Castanon ◽  
Juan P. Loyola-Rodriguez ◽  
Nuria Patiño-Marin ◽  
...  
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Dental Fluorosis ◽  
In Vitro Study ◽  
Mean Value ◽  
Fixed Appliance ◽  
Adhesive Remnant Index ◽  
The Mean ◽  
Electronic Microscope

Abstract Objective: To study the shear bond strength (SBS), sites of failure, and micromorphology of bonded molar tubes used on teeth affected by dental fluorosis. Materials and Methods: This in vitro study included 140 first molars classified according to Dean's index for dental fluorosis. Samples were divided into seven groups: (1) healthy teeth etched for 15 seconds, (2) teeth with moderate fluorosis (MOF) etched for 15 seconds, (3) teeth with MOF etched for 150 seconds, (4) teeth with MOF microabrasion etched for 15 seconds, (5) teeth with severe fluorosis (SEF) etched for 15 seconds, (6) teeth with SEF etched for 150 seconds, and (7) teeth with SEF microabrasion etched for 15 seconds. All samples were incubated and were then submitted to the SBS test and evaluated with the modified adhesive remnant index (ARI) and analyzed by using a scanning electronic microscope. Results: The SBS mean value for healthy enamel was 20 ± 10.2 MPa. For the group with MOF, the etched 150-second mean value was the highest (19 ± 7.6 MPa); for the group with SEF treated with microabrasion and etched for 15 seconds, the mean value was (13 ± 4.1 MPa). Significant differences (P ≤ .05) were found in the ARI between healthy and fluorosed groups. Conclusions: Fluorotic enamel affects the adhesion of bonded molar tubes. The use of overetching in cases of MOF and the combination of microabrasion and etching in SEF provides a suitable adhesion for fixed appliance therapy.

scholarly journals An in vitro comparison of the force decay generated by different commercially available elastomeric chains and NiTi closed coil springs

2007 ◽  
Vol 21 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Ana Cristina Soares Santos ◽  
André Tortamano ◽  
Sandra Regina Frazatto Naccarato ◽  
Gladys Cristina Dominguez-Rodriguez ◽  
Julio Wilson Vigorito
Keyword(s):  
Artificial Saliva ◽  
Testing Machine ◽  
In Vitro Study ◽  
Original Length ◽  
Force Decay ◽  
Decay Pattern ◽  
The Mean ◽  
Initial Force ◽  
Elastomeric Chains

This in vitro study was designed to compare the forces generated by commercially available elastomeric chains and NiTi closed coil springs, and to determine their force decay pattern. Forty elastomeric chains and forty NiTi closed coil springs were divided into 4 groups according to the following manufacturers: (1) Morelli®, (2) Abzil®, (3) TP Orthodontics® and (4) American Orthodontics®. The specimens were extended to twice their original length and stored in artificial saliva at 37°C. Initial force was measured by means of an Instron universal testing machine and then at 1, 4, 7, 14, 21, and 28 days. The results revealed that the elastomeric chains delivered a mean initial force of 347 g for Morelli®, 351 g for American Orthodontics®, 402 g for Abzil®, and 404 g for TP Orthodontics®. The NiTi closed coil springs generated a mean initial force of 196 g for American Orthodontics®, 208 g for TP Orthodontics®, 216 g for Abzil®, and 223 g for Morelli®. The mean percentage of force decay observed after 28 days for the elastomeric chains was 37.4% for TP Orthodontics®, 48.1% for American Orthodontics®, 65.4% for Morelli®, and 71.6% for Abzil®. After 28 days, the NiTi closed coil springs presented a mean percentage of force decay of 22.6% for American Orthodontics®, 29.8% for Abzil®, 30.6% for Morelli®, and 45.8% for TP Orthodontics®. At the end of the study, significant differences were observed between the elastomeric chains and the NiTi closed coil springs. The results indicated that the studied NiTi closed coil springs are more adequate for dental movement than the elastomeric chains.

scholarly journals Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) terhadap Staphylococcus aureus dan Escherichia coli secara In Vitro

2012 ◽  
Vol 13 (1) ◽  
pp. 63
Author(s):  
Taswin Yacob ◽  
Rita Endriani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Senna Alata ◽  
Treatment Of Infection ◽  
Range Test

The benefit and efficacy of ketepeng cina (Senna alata) in the treatment of infection has shown that have antibacterialactivity, inhibiting and killing bacteria that cause infection. The objective of this study was evaluate the antibacterialactivity of ketepeng cina against Staphylococcus aureus dan Escherichia coli in vitro. This study was a laboratoryexperimental research which use completely randomized design with diffusion method. Ethanol extract of Sennaalata leaves devided into 4 doses, i.c. 100, 50, 25 and 12.5. Amoxiclave were used as positive control and aquadestnegative control. The data were analyzed by Analysis of Varian continued with Duncan’s Multiple Range Test. Theresult of this study showed that antimicrobial activity of ethanol extract Senna alata leaves inhibited the growth ofStaphylococcus aureus, but not Escherichia coli. The optimum effect was showed given by the concentration 100at 17.7 mm.

scholarly journals Penggunaan Bahan Organik dan Kombinasinya dalam Formulasi Biofungisida Berbahan Aktif Jamur Trichoderma pseudokoningii Rifai. untuk Menghambat Jamur Ganoderma boninense Pat. secara in vitro

2017 ◽  
Vol 16 (2) ◽  
pp. 79
Author(s):  
Yetti Elfina ◽  
Muhammad Ali ◽  
Rachmad Saputra
Keyword(s):  
Rice Husk ◽  
Ganoderma Boninense ◽  
Organic Matters ◽  
Randomized Design ◽  
Shrimp Shell ◽  
Trichoderma Pseudokoningii ◽  
Completely Randomized Design ◽  
Rot Disease ◽  
Range Test

Trichoderma pseudokoningii has been applied as a biocontrol agent against fungal plant pathogen, such as Ganoderma boninense, the cause of stem rot disease on palm oil plants. To be more effectively applicable in the field, some experiments have been employed to formulate T. pseudokoningii in a biofungicide formulation amended with organic matter as its main nutrient resource, zealot as a carrier agent and cocoyam powder as a mixture agent. A research has been conducted to study the effect of various organic matters and their combinations in a biofungicide formulation of T. pseudokoningii on growth inhibition to G. boninense in-vitro and to obtain the best organic matters and their combinations in enhancing the growth of T. pseudokoningii and yet inhibiting G. boninense. The research has been conducted in the Laboratory of Plant Pathology, Agriculture Faculty, University of Riau from May to August 2012, using a completely randomized design consisting of 15 treatments (bagasse, rice husk, shrimp shell, dregs, and their combinations) and 3 replications. The data were statistically analyzed using analysis of variance, followed by Duncan’s New Multiple Range Test (DNMRT). The results indicated that organic matters and their combinations in the biofungicide formulation significantly affected the antagonistic capacity of T. pseudokoningii in inhibiting the growth of G. boninense in vitro. Rice husk, bagases, bagasse+rice husks, and bagasse+dregs were the best organic matters in enhancing the growth of T. pseudokoningii and its capacity to inhibit G. boninense in-vitro. It can also be concluded that shrimp shell, bagasse+shrimp shell, rice husk+shrimp shell, shrimp shell+dregs and rice husk+shrimp shell+dregs totally inhibited the growth of T. pseudokoningii in the biofungicide formulation.

scholarly journals Analisis Daya Hambat Ekstrak Daun Sirih Hijau (Piper betle L.) terhadap Mikroorganisme Indikator Mastitis

2020 ◽  
Vol 4 (2) ◽  
pp. 230-236
Author(s):  
Dicky Rizaldi Rambe ◽  
Allaily Allaily ◽  
Cut Intan Novita
Keyword(s):  
Leaf Extract ◽  
Agricultural Product ◽  
Piper Betle ◽  
Product Analysis ◽  
E Coli ◽  
Betel Leaf ◽  
Randomized Design ◽  
Inhibitory Analysis ◽  
Analysis Laboratory ◽  
Range Test

Abstrak. Penelitian tentang Analisis Daya Hambat Ekstrak Daun Sirih Hijau (Piper betle L.) terhadap Mikroorganisme Indikator Mastitis telah dilaksanakan di Laboratorium Ilmu dan Teknologi Pengolahan Susu dan Laboratorium Analisis Pangan dan Hasil Pertanian yang dilaksanakan pada 7 September sampai dengan 10 November 2018. Penelitian ini terdiri dari 4 perlakuan dan 5 ulangan. Data yang diperoleh selanjutnya akan diolah menggunakan Rancangan Acak Lengkap, jika didapatkan hasil yang berbeda maka akan dilanjutkan dengan uji Duncan. Perlakuan R1 yaitu penambahan ekstrak daun sirih hijau 15%, R2 yaitu penambahan ekstrak daun sirih hijau 30%, R3 yaitu penambahan ekstrak daun sirih hijau 45% dan R4 yaitu penambahan ekstrak daun sirih hijau 60%. Hasil penelitian menunjukkan bahwa ekstrak daun sirih hijau dengan konsentrasi 15%, 30%, 45% dan 60% mampu menghambat E. coli dan S. aureus. Berdasarkanhasil penelitian ini dapat disimpulkan bahwa ekstrak daun sirih hijau mampu menghambat pertumbuhan E. coli dan S. aureus.Inhibiting Power Analysis of Green Betel Leaf Extract (Piper betle L.) against Microorganism Indicator MastitisAbstract. Research on Inhibitory Analysis of Green Betel Leaf Extract (Piper betle L.) against Mastitis causing Microorganism has been conducted in Milk Processing Science and Technology Laboratory and  Food and Agricultural Product Analysis Laboratory held on September 7 to November 10, 2018. This research consisted of 4 treatments and 5 replications. The data obtained will then be processed using a Completely Randomized Design, if different results are obtained it will be followed by the Duncan Multiple Range Test. The treatment of R1 is the addition of 15% green betel leaf extract, R2, which is the addition of 30% green betel leaf extract, R3, namely the addition of 45% green betel leaf extract and R4 which is the addition of 60% green betel leaf extract. The results showed that green betel leaf extract with a concentration of 15%, 30%, 45% and 60% was able to inhibit E. coli and S. aureus. Based on the results of this study it can be concluded that green betel leaf extract can inhibit the growth of E. coli and S. aureus.

scholarly journals THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 39
Author(s):  
Salma Nabila ◽  
Endah Budi Irawati ◽  
Rina Srilestari
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Fresh Weight ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Range Test ◽  
Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety  of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on  fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

scholarly journals Promoting Tuber Formation In Vitro with Benzyl Amino Purine and Paclobutrazol at Different Concentrations

Akta Agrosia ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 29-35
Author(s):  
Usman Kris Joko Suharjo ◽  
Hasanudin Hasanudin ◽  
Tunjung Pamekas ◽  
Hesti Pujiwati ◽  
Alyi Vanturini
Keyword(s):  
Poor Quality ◽  
Tuber Formation ◽  
Potato Tubers ◽  
Seed Sources ◽  
Randomized Design ◽  
Plant Culture ◽  
Wet Weight ◽  
Completely Randomized Design ◽  
Range Test

A common problem related to the production of potatoes in Indonesia is the use of poor quality seed sources and declining seeds. Plant tissue culture is one of the best methods used for the provision of quality seeds. This study aims to establish the best combination of BAP concentration and the concentration of paclobutrazol in spurring the growth of cuttings and initiation of potato tubers in vitro.This research was conducted in November 2017 until June 2018 at Agronomy Laboratory of Biotechnology Division and Plant Culture Network of Agriculture Faculty of Bengkulu University. The  research  design  used  was  Completely  Randomized  Design  (CRD)  which  consist  of  18 combination of BAP treatment and Paclobutrazol was repeated 6 times. The concentration of BAP used was 0 mg / l, 5 mg / l, 10 mg / l and the concentration of Paclobutrazol 0 mg / l; 2.5 mg / l; 5 mg / l; 7.5 mg / l; 10 mg / l; 12.5 mg / l. The data of the research were analyzed by F test of 5% level and if there were different between treatments followed by Duncan's Multiple Range Test (DMRT) test of5% level. The results showed that the combination of BAP and  Paclobutrazol concentration had significant effect on all observed variables. Treatment of 5 mg / l BAP and 7.5 mg / l Paclobutrazol is the best medium in inducing potato micro tube as indicated by the fastest tuber formation time, highest percentage of productive crops, largest number of tubers per bottle, number of tubers per productive plant, tuber diameter the largest and highest wet weight of tuber per bottle. Keywords : in vitro, BAP, paclobutrazol, Potato

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