Black cardamom essential oil prevents Escherichia coli O157:H7 and Salmonella Typhimurium JSG 1748 biofilm formation through inhibition of quorum sensing

2020 ◽  
Author(s):  
Abdullah ◽  
Ammar Algburi ◽  
Ali Asghar ◽  
Qingrong Huang ◽  
Warda Mustfa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Essential Oil ◽  
Salmonella Typhimurium ◽  
Biofilm Formation ◽  
Escherichia Coli O157

Quorum sensing is crucial to Escherichia coli O157:H7 biofilm formation under static or very slow laminar flow conditions

2016 ◽  
Vol 10 (3) ◽  
pp. 241-249 ◽  
Author(s):  
Jeesun Lim ◽  
Kang-Mu Lee ◽  
Chan Yong Park ◽  
Han Vit Kim ◽  
Younghoon Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Laminar Flow ◽  
Biofilm Formation ◽  
Escherichia Coli O157 ◽  
Flow Conditions ◽  
Laminar Flow Conditions

scholarly journals Anti-biofilm Potential of Elletaria cardamomum Essential Oil Against Escherichia coli O157:H7 and Salmonella Typhimurium JSG 1748

2021 ◽  
Vol 12 ◽  
Author(s):  
Abdullah ◽  
Ali Asghar ◽  
Ammar Algburi ◽  
Qingrong Huang ◽  
Talha Ahmad ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Essential Oil ◽  
Foodborne Pathogens ◽  
Biofilm Formation ◽  
Mutagenic Activity ◽  
Escherichia Coli O157 ◽  
Safe Alternative ◽  
Mutagenicity Assay ◽  
Green Cardamom ◽  
Alternative Antimicrobials

Foodborne pathogens, microbial recurrent infections, and antibiotic resistance have driven researchers to explore natural compounds as safe alternative antimicrobials. In this study, the chemical profile, antimicrobial, and mutagenic activities of the Elletaria cardamomum essential oil were investigated. GC-MS analysis identified the major bioactive components as α-terpinyl acetate, 1,8-cineole, linalool acetate, and sabinene, at concentrations of 34.95, 25.30, 8.13, and 5.48% respectively, of the essential oil’s content. Regarding antimicrobial activity, the minimum inhibitory concentration of green cardamom essential oil was 1% against Escherichia coli O157:H7 and Pseudomonas aeruginosa ATCC 14213. Green cardamom essential oil, when used at concentrations of 0.015, 0.031, 0.062, and 0.125% (v/v) prevented biofilm formation of Escherichia coli O157:H7 by 64.29, 65.98, 70.41, and 85.59%, respectively. Furthermore, these concentrations inhibited 6.13, 45.50, 49.45, and 100%, respectively, of the Salmonella Typhimurium JSG 1748 biofilm. A mutagenicity assay confirmed that green cardamom essential oil has no demonstrable mutagenic activity against the tested strains. The study’s findings suggest that green cardamom derived bioactive compounds are safe organic antimicrobials, effective in controlling biofilm formation by Gram-negative pathogens. Moreover, such compounds could possibly be used in the food industry (e.g., bakery, dairy, meat, and other food products) as a safe alternative to chemical preservatives (antimicrobials) to enhance shelf life by improving the antimicrobial status while at the same time imparting a pleasant and appealing aroma for consumers.

scholarly journals Inhibitory effects of lactic and malic organic acids on autoinducer type 2 (AI-2) quorum sensing of Escherichia coli O157:H7 and Salmonella Typhimurium

LWT ◽  
2016 ◽  
Vol 66 ◽  
pp. 560-564 ◽  
Author(s):  
Ahmad Almasoud ◽  
Navam Hettiarachchy ◽  
Srinivas Rayaprolu ◽  
Dinesh Babu ◽  
Young Min Kwon ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Organic Acids ◽  
Salmonella Typhimurium ◽  
Escherichia Coli O157 ◽  
Inhibitory Effects

scholarly journals Inhibition of Glucosyltransferase Activity and Glucan Production as an Antibiofilm Mechanism of Lemongrass Essential Oil against Escherichia coli O157:H7

Antibiotics ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 102 ◽  
Author(s):  
Luis A. Ortega-Ramirez ◽  
M. Melissa Gutiérrez-Pacheco ◽  
Irasema Vargas-Arispuro ◽  
Gustavo A. González-Aguilar ◽  
Miguel A. Martínez-Téllez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Essential Oil ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Escherichia Coli O157 ◽  
Cymbopogon Citratus ◽  
Docking Analysis ◽  
E Coli ◽  
Lemongrass Essential Oil

The resistance of Escherichia coli O157:H7 to disinfection is associated with its ability to form biofilms, mainly constituted by glucans produced by glucosyltransferases. Citral and geraniol, terpenes found in the essential oil of Cymbopogon citratus (EO), have proven antibacterial activity against planktonic E. coli; however, no information was found about their efficacy and mode of action against E. coli biofilms. Therefore, the inhibitory effect of C. citratus EO, citral, and geraniol on glucans production and glucosyltransferase activity as anti-biofilm mechanism against E. coli was evaluated. EO, citral, and geraniol inhibited the planktonic growth of E. coli (minimal inhibitory concentration or MIC= 2.2, 1.0, and 3.0 mg/mL, respectively) and the bacterial adhesion (2.0, 2.0, and 4.0 mg/mL, respectively) on stainless steel. All compounds decreased the glucans production; citral and geraniol acted as uncompetitive inhibitors of glucosyltransferase activity (The half maximal inhibitory concentrations or IC50 were 8.5 and 6.5 µM, respectively). The evidence collected by docking analysis indicated that both terpenes could interact with the helix finger of the glucosyltransferase responsible for the polymer production. In conclusion, C. citratus EO, citral, and geraniol inhibited glucosyltransferase activity, glucans production, and the consequent biofilm formation of E. coli O157:H7.

scholarly journals DYNAMICS OF LISTERIA MONOCYTOGENES, SALMONELLA TYPHIMURIUM ESCHERICHIA COLI O157: H7 DURING THE PRODUCTION OF ‘NDUJA

2011 ◽  
Vol 1 (1zero) ◽  
pp. 141 ◽  
Author(s):  
E. Oliverio ◽  
G. Finazzi ◽  
P. Daminelli ◽  
P. Monastero ◽  
P. Boni
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Escherichia Coli O157
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