scholarly journals Gold nanoparticles synthesized by Brassica oleracea (Broccoli) acting as antimicrobial agents against human pathogenic bacteria and fungi

2015 ◽  
Vol 6 (4) ◽  
pp. 467-473 ◽  
Author(s):  
Prakash Piruthiviraj ◽  
Anita Margret ◽  
Poornima Priyadharsani Krishnamurthy
Keyword(s):  
Gold Nanoparticles ◽  
Brassica Oleracea ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Human Pathogenic Bacteria ◽  
Bacteria And Fungi

scholarly journals Antimicrobial Activity and Chemical Constitution of the Crude, Phenolic-Rich Extracts of Hibiscus sabdariffa, Brassica oleracea and Beta vulgaris

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4280 ◽  
Author(s):  
Seham Abdel-Shafi ◽  
Abdul-Raouf Al-Mohammadi ◽  
Mahmoud Sitohy ◽  
Basma Mosa ◽  
Ahmed Ismaiel ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Beta Vulgaris ◽  
Pathogenic Bacteria ◽  
Aspergillus Ochraceus ◽  
Total Phenolic ◽  
Klebsiella Pneumonia ◽  
Hibiscus Sabdariffa ◽  
Cell Organelles ◽  
Cell Shapes ◽  
Bacteria And Fungi

Crude, phenolic-rich extracts (CPREs) were isolated from different sources, such as Hibiscus sabdariffa (H. sabdariffa), Brassica oleracea var. capitata f. rubra (B. oleracea) and Beta vulgaris (B. vulgaris) and characterized. These CPREs showed potential antibacterial and antifungal activities. H. sabdariffa CPRE (HCPRE) is the most potent, as it inhibited all tested bacteria and fungi. Total anthocyanins content (TAC), total phenolic content (TPC) and total flavonoid content (TFC) were estimated in all three CPREs. H. sabdariffa contained 4.2 mg/100 g TAC, 2000 mg/100 g of TPC and 430 mg/100 g of TFC in a dry weight sample. GC–MS analysis of HCPRE showed 10 different active compounds that have antimicrobial effects against pathogenic bacteria and fungi, especially alcoholic compounds, triazine derivatives and esters. Scanning and transmission electron microscopy images of Staphylococcus aureus DSM 1104 and Klebsiella pneumonia ATCC 43816 treated with HCPRE (50 μg/mL) exhibited signs of asymmetric, wrinkled exterior surfaces, cell deformations and loss of cell shapes; and adherence of lysed cell content led to cell clumping, malformations, blisters, cell depressions and diminished cell numbers. This indicates death of bacterial cells and loss of cell contents. Aspergillus ochraceus EMCC516 (A. ochraceus, when treated with 100 μg/mL of HCPRE showed irregular cell organelles and cell vacuolation.

Biogenic Synthesis of Gold Nanoparticles and Their Antimicrobial Activities

2020 ◽  
pp. 216-233
Author(s):  
Snežana Radisavljević ◽  
Biljana Petrović
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Antibacterial Activity ◽  
Biomedical Applications ◽  
Food Packaging ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Bactericidal Effect ◽  
Antimicrobial Activities ◽  
Physiochemical Properties

Gold nanoparticles (AuNPs) are widely used in biomedical applications, especially diagnostic and drug delivery. The antibacterial activity of nanoparticles depends on the dimensions of the particles. AuNPs may associate with the surface of the cell membrane and cause disorder such as respiration and permeability. The method of binding of particles for bacteria depends on their surface available for interaction. Smaller particles which have the larger surface area available for interaction will show better bactericidal effect than the larger particles. Useful antibacterial agents should also be toxic to various pathogenic bacteria with the ability to coat different surfaces like biomaterials, devices, textiles, food packaging, and so on. The biological and physiochemical properties of synthesized AuNPs have impact on the use of gold nanoparticles like antimicrobial agents, especially for water purification, as well as other biomedical applications.

scholarly journals Synthesis and Biological Activity Evaluation of Novel 5-Methyl-7-phenyl-3H-thiazolo[4,5-b]pyridin-2-ones

2021 ◽  
Vol 89 (4) ◽  
pp. 52
Author(s):  
Andrii Lozynskyi ◽  
Yulian Konechnyi ◽  
Julia Senkiv ◽  
Ihor Yushyn ◽  
Dmytro Khyluk ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Inhibitory Effect ◽  
Binding Energies ◽  
Moderate Activity ◽  
Reference Drug ◽  
Potent Inhibitory Effect ◽  
Bacteria And Fungi ◽  
Cytotoxicity Effects ◽  
Further Development

A series of 5-methyl-7-phenyl-3H-thiazolo[4,5-b]pyridin-2-ones has been designed, synthesized, and characterized by spectral data. Target compounds were screened for their antimicrobial activity against some pathogenic bacteria and fungi, and most of them showed moderate activity, especially compound 3g, which displayed the potent inhibitory effect against Pseudomonas aeruginosa and Escherichia coli with MIC value of 0.21 μM. The active thiazolopyridine derivatives 3c, 3f, and 3g were screened for their cytotoxicity effects on HaCat, Balb/c 3T3 cells using MTT assay, which revealed promising results. In silico assessment for compounds 3c, 3f, and 3g also revealed suitable drug-like parameters and ADME properties. The binding interactions of the most active compound 3g were performed through molecular docking against MurD and DNA gyrase, with binding energies and an inhibitory constant compared to the reference drug ciprofloxacin. The tested thiazolo[4,5-b]pyridines constitute an exciting background for the further development of new synthetic antimicrobial agents.

scholarly journals Effect of Plasmonic Gold Nanoprisms on Biofilm Formation and Heat Shock Proteins Expression in Human Pathogenic Bacteria

2021 ◽  
Vol 14 (12) ◽  
pp. 1335
Author(s):  
Rihab Lagha ◽  
Fethi Ben Abdallah ◽  
Amine Mezni ◽  
Othman M. Alzahrani
Keyword(s):  
Gold Nanoparticles ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Western Blot ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Biofilm Inhibition ◽  
Human Pathogenic Bacteria ◽  
Gold Nanoprisms ◽  
Shock Proteins

Gold nanoparticles have gained interest in biomedical sciences in the areas of nano-diagnostics, bio-labeling, drug delivery, and bacterial infection. In this study, we examined, for the first time, the antibacterial and antibiofilm properties of plasmonic gold nanoprisms against human pathogenic bacteria using MIC and crystal violet. In addition, the expression level of GroEL/GroES heat shock proteins was also investigated by western blot. Gold nanoparticles were characterized by TEM and EDX, which showed equilateral triangular prisms with an average edge length of 150 nm. Antibacterial activity testing showed a great effect of AuNPs against pathogenic bacteria with MICs values ranging from 50 μg/mL to 100 μg/mL. Nanoparticles demonstrated strong biofilm inhibition action with a percentage of inhibition ranging from 40.44 to 82.43%. Western blot analysis revealed that GroEL was an AuNPs-inducible protein with an increase of up to 66.04%, but GroES was down-regulated with a reduction of up to 46.81%. Accordingly, plasmonic gold nanoprisms, could be a good candidate for antibiotics substitution in order to treat bacterial infections.

Antipyrine cationic surfactants capping silver nanoparticles as potent antimicrobial agents against pathogenic bacteria and fungi

2017 ◽  
Vol 243 ◽  
pp. 572-583 ◽  
Author(s):  
I. Aiad ◽  
Magda I. Marzouk ◽  
Soheir A. Shaker ◽  
Nagwa E. Ebrahim ◽  
Ali A. Abd-Elaal ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cationic Surfactants ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Bacteria And Fungi

scholarly journals Antimicrobial Activity of Five Yemeni Medicinal Plants against Selected Human Pathogenic Bacteria and Fungi

2019 ◽  
Vol 10 (10) ◽  
pp. 1699-1707
Author(s):  
Nagi Al-Haj ◽  
Alariqi Reem ◽  
Hassan Al-Shamahy ◽  
Khaled Al-Moyed ◽  
Saleh S. Bahaj ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Human Pathogenic Bacteria ◽  
Bacteria And Fungi

scholarly journals Antimicrobial activity of the essential oil of Phlomis bracteosa

1970 ◽  
Vol 9 (9) ◽  
pp. 63-65 ◽  
Author(s):  
RK Joshi ◽  
Veena Pande ◽  
BC Thakuri
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Traditional Medicine ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Human Pathogenic Bacteria ◽  
Aerial Parts ◽  
Bacteria And Fungi

The essential oil was isolated from the aerial parts of Phlomis bracteosa Royle ex Benth., a hairy erect herb. The leaves and flowers of this species are used as carminative, stimulant and locally known as ‘Ballikotu, Calba, Salba’ in Turkish traditional medicine. The antimicrobial activity of Phlomis bracteosa was studied using well diffusion method. The activity was tested against human pathogenic bacteria and fungi at different concentrations (0.25 μg/ml, 0.125 μg/ml and 0.062 μg/ml) of the essential oil. Keywords: Phlomis bracteosa; Lamiaceae; Antimicrobial activity DOI: http://dx.doi.org/10.3126/sw.v9i9.5521 SW 2011; 9(9): 63-65

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