scholarly journals Optimization of cultivation conditions for biotechnological production of lipid by Rhodotorula kratochvilovae (syn, Rhodosporidium kratochvilovae) SY89 for biodiesel preparation

3 Biotech ◽  
2017 ◽  
Vol 7 (2) ◽  
Author(s):  
Tamene Milkessa Jiru ◽  
Marizeth Groenewald ◽  
Carolina Pohl ◽  
Laurinda Steyn ◽  
Nicholas Kiggundu ◽  
...  
Keyword(s):  
Cultivation Conditions ◽  
Biotechnological Production ◽  
Rhodosporidium Kratochvilovae

scholarly journals New insight into chondroitin and heparosan-like capsular polysaccharide synthesis by profiling of the nucleotide sugar precursors

2017 ◽  
Vol 37 (1) ◽  
Author(s):  
Odile Francesca Restaino ◽  
Irene di Lauro ◽  
Rosaria Di Nuzzo ◽  
Mario De Rosa ◽  
Chiara Schiraldi
Keyword(s):  
Growth Phase ◽  
Capsular Polysaccharide ◽  
Surrounding Medium ◽  
Stationary Growth Phase ◽  
Outer Cell ◽  
Cultivation Conditions ◽  
Biotechnological Production ◽  
Electrophoresis Method ◽  
Set Up ◽  
First Time

Escherichia coli K4 and K5 capsular polysaccharides (K4 and K5 CPSs) have been used as starting material for the biotechnological production of chondroitin sulfate (CS) and heparin (HP) respectively. The CPS covers the outer cell wall but in late exponential or stationary growth phase it is released in the surrounding medium. The released CPS concentration was used, so far, as the only marker to connect the strain production ability to the different cultivation conditions employed. Determining also the intracellular UDP-sugar precursor concentration variations, during the bacterial growth, and correlating it with the total CPS production (as sum of the inner and the released ones), could help to better understand the chain biosynthetic mechanism and its bottlenecks. In the present study, for the first time, a new capillary electrophoresis method was set up to simultaneously analyse the UDP-glucose (UDP-Glc), UDP-galactose (UDP-Gal), UDP-N-acetylgalactosamine (UDP-GalNAc), UDP-N-acetylglucosamine (UDP-GlcNAc) and UDP-glucuronic acid (UDP-GlcA) and the inner CPS portion, extracted at the same time from the bacterial biomasses; separation was performed at 18°C and 18 kV with a borate-based buffer and detection at 200 nm. The E. coli K4 and K5 UDP-sugar pools were profiled, for the first time, at different time points of shake flask growths on a glycerol-containing medium and on the same medium supplemented with the monosaccharide precursors of the CPSs: their concentrations varied from 0.25 to 11 μM·gcdw−1, according to strain, the type of precursor, the growth phase and the cultivation conditions and their availability dramatically influenced the total CPS produced.

Influence of Planting Density on Root Spatio-Temporal Distribution of Different Types of Maize under High-Yielding Cultivation Conditions

2013 ◽  
Vol 38 (7) ◽  
pp. 1286-1294 ◽  
Author(s):  
Zong-Xin LI ◽  
Yuan-Quan CHEN ◽  
Qing-Cheng WANG ◽  
Kai-Chang LIU ◽  
Wang-Sheng GAO ◽  
...  
Keyword(s):  
Temporal Distribution ◽  
Planting Density ◽  
Cultivation Conditions ◽  
Different Types ◽  
Spatio Temporal

scholarly journals Expansion of EasyClone-MarkerFree toolkit for Saccharomyces cerevisiae genome with new integration sites

2021 ◽  
Author(s):  
Mahsa Babaei ◽  
Luisa Sartori ◽  
Alexey Karpukhin ◽  
Dmitrii Abashkin ◽  
Elena Matrosova ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Recombinant Dna ◽  
Green Fluorescence Protein ◽  
Cellular Growth ◽  
Green Fluorescence ◽  
Biotechnological Production ◽  
Yeast Saccharomyces Cerevisiae ◽  
Integration Sites ◽  
Fluorescence Protein ◽  
Integration Efficiency

Abstract Biotechnological production requires genetically stable recombinant strains. To ensure genomic stability, recombinant DNA is commonly integrated into the genome of the host strain. Multiple genetic tools have been developed for genomic integration into baker's yeast Saccharomyces cerevisiae. Previously, we had developed a vector toolkit EasyClone-MarkerFree for stable integration into eleven sites on chromosomes X, XI, and XII of S. cerevisiae. The markerless integration was enabled by CRISPR-Cas9 system. In this study, we have expanded the kit with eight additional intergenic integration sites located on different chromosomes. The integration efficiency into the new sites was above 80%. The expression level of green fluorescence protein (gfp) for all eight sites was similar or above XI-2 site from the original EasyClone-MarkerFree toolkit. The cellular growth was not affected by the integration into any of the new eight locations. The eight-vector expansion kit is available from AddGene.

scholarly journals Cultivation Conditions Change Aroma Volatiles of Strawberry Fruit

Horticulturae ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 81
Author(s):  
Yunduan Li ◽  
Yuanyuan Zhang ◽  
Xincheng Liu ◽  
Yuwei Xiao ◽  
Zuying Zhang ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Major Contributor ◽  
Fragaria Ananassa ◽  
Strawberry Fruit ◽  
Cultivation Conditions ◽  
Soil Culture ◽  
Aroma Volatiles ◽  
Ms Analysis ◽  
Significant Difference ◽  
Strawberry Cultivars

Volatile compounds principally contribute to flavor of strawberry (Fragaria × ananassa) fruit. Besides to genetics, cultivation conditions play an important role in fruit volatile formation. Compared to soil culture as control, effects of substrate culture on volatile compounds of two strawberry cultivars (‘Amaou’ and ‘Yuexin’) were investigated. GC-MS analysis revealed significant difference in volatile contents of ‘Amaou’ strawberry caused by substrate culture. No significant effect was observed for cultivar ‘Yuexin’. For ‘Amaou’ strawberry from soil culture produced higher volatile contents compared with substrate culture. This difference is contributed by high contents of esters, lactones, ketones, aldehydes, terpenes, hydrocarbons, acids, furans and phenols in ‘Amaou’ strawberry fruit from soil culture. Furanones, beta-linalool, trans-Nerolidol and esters are major contributor to strawberry aroma, whose contents are higher in soil culture planted fruit when compared to substrate culture. Moreover, strawberry fruit from soil culture had higher transcripts related to volatile biosynthesis were observed, including FaQR, FaOMT, FaNES1, FaSAAT and FaAAT2.

scholarly journals Self-Configuring CVS to Discriminate Rocket Leaves According to Cultivation Practices and to Correctly Attribute Visual Quality Level

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1353
Author(s):  
Michela Palumbo ◽  
Bernardo Pace ◽  
Maria Cefola ◽  
Francesco Fabiano Montesano ◽  
Francesco Serio ◽  
...  
Keyword(s):  
Fruits And Vegetables ◽  
Irrigation Management ◽  
Classification Performance ◽  
Quality Level ◽  
Cultivation Conditions ◽  
Computer Vision Systems ◽  
Soilless Cultivation ◽  
Fertilization Level ◽  
Non Destructive

Computer Vision Systems (CVS) represent a contactless and non-destructive tool to evaluate and monitor the quality of fruits and vegetables. This research paper proposes an innovative CVS, using a Random Forest model to automatically select the relevant features for classification, thereby avoiding their choice through a cumbersome and error-prone work of human designers. Moreover, three color correction techniques were evaluated and compared, in terms of classification performance to identify the best solution to provide consistent color measurements. The proposed CVS was applied to fresh-cut rocket, produced under greenhouse soilless cultivation conditions differing for the irrigation management strategy and the fertilization level. The first aim of this study was to objectively estimate the quality levels (QL) occurring during storage. The second aim was to non-destructively, and in a contactless manner, identify the cultivation approach using the digital images of the obtained product. The proposed CVS achieved an accuracy of about 95% in QL assessment and about 65–70% in the discrimination of the cultivation approach.

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