Introgression of QTLs determining sorghum downy mildew (SDM) resistance into elite maize line UMI 79 through marker-assisted backcross breeding (MABC)

2020 ◽  
Vol 49 (2) ◽  
pp. 159-165 ◽  
Author(s):  
K. Sumathi ◽  
K. N. Ganesan ◽  
P. Aarthi ◽  
V. Sruthymenon ◽  
S. Devasree ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Maize Line ◽  
Sorghum Downy Mildew ◽  
Backcross Breeding ◽  
Marker Assisted Backcross Breeding

scholarly journals Marker-Assisted Pyramiding of Downy Mildew-Resistant Gene Ppa3 and Black Rot-Resistant Gene Xca1bo in Popular Early Cauliflower Variety Pusa Meghna

2021 ◽  
Vol 12 ◽  
Author(s):  
Partha Saha ◽  
Chandrika Ghoshal ◽  
Namita Das Saha ◽  
Aakriti Verma ◽  
Mohita Srivastava ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Black Rot ◽  
Breeding Method ◽  
Resistant Gene ◽  
Yield And Quality ◽  
Resistant Genes ◽  
Backcross Breeding ◽  
Hybridization Programme ◽  
Marker Assisted Backcross Breeding

Cauliflower is an important extensively grown cool season vegetable in India. Black rot and downy mildew are major devastating diseases reducing yield and quality of the crop. To tackle these through host plant resistance, a marker-assisted backcross breeding method was followed to pyramid a black rot-resistant gene (Xca1bo) and a downy mildew-resistant gene (Ppa3) from donors BR-161 and BR-2, respectively, into the background of Pusa Meghna cauliflower cultivar. Marker-assisted backcross breeding was followed up to BC2 generation using SCAR marker ScOPO-04833 and SSR marker BoGMS0624 for black rot and downy mildew resistance genes in foreground selection, respectively. In background selection, at each stage of backcrossing, 47 parental polymorphic SSR markers were used. The graphical genotyping of the five two-gene (Xca1boXca1boPpa3Ppa3) homozygous BC2F2 plants showed an average recovery of 85.44% of the Pusa Meghna genome with highest genome recovery of 91.7%. The genome contribution of donor parents (BR-161 and BR-2) was 8.26 with 6.34% of residual heterozygousity. The backcross derived pyramided lines BC2F2:3-7-16 and BC2F2:3-7-33 showed high resistance to both the diseases and exhibited higher yield and vitamin C content as compared with recipient parent Pusa Meghna. It is, therefore, evident from this study that resistant genes can be introgressed successfully into a Pusa Meghna cultivar without any yield penalty, benefitting farmers with reduced input cost and consumers with chemical residue free produce. Besides, the pyramided lines carrying dominant resistant genes can be exploited in a hybridization programme to develop hybrid(s) in cauliflower.

Improvement of Bacterial Blight Resistance in Rice Cultivars Jyothi and IR50 via Marker-Assisted Backcross Breeding

2008 ◽  
Vol 21 (1) ◽  
pp. 101-116 ◽  
Author(s):  
S. Bharathkumar ◽  
R. S. David Paulraj ◽  
P. V. Brindha ◽  
S. Kavitha ◽  
S. S. Gnanamanickam
Keyword(s):  
Bacterial Blight ◽  
Blight Resistance ◽  
Rice Cultivars ◽  
Bacterial Blight Resistance ◽  
Backcross Breeding ◽  
Marker Assisted Backcross Breeding

Genetic analysis of resistance to sorghum downy mildew and Rajasthan downy mildew in maize (Zea mays L.)

2004 ◽  
Vol 89 (2-3) ◽  
pp. 379-387 ◽  
Author(s):  
Sudha K Nair ◽  
B.M Prasanna ◽  
R.S Rathore ◽  
T.A.S Setty ◽  
R Kumar ◽  
...  
Keyword(s):  
Zea Mays ◽  
Genetic Analysis ◽  
Downy Mildew ◽  
Zea Mays L ◽  
Sorghum Downy Mildew

scholarly journals Recovery of Recurrent Parent Genome in a Marker-Assisted Backcrossing Against Rice Blast and Blight Infections Using Functional Markers and SSRs

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1411
Author(s):  
Samuel Chibuike Chukwu ◽  
Mohd Y. Rafii ◽  
Shairul Izan Ramlee ◽  
Siti Izera Ismail ◽  
Yusuff Oladosu ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Blast Resistance ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Functional Markers ◽  
Recurrent Parent ◽  
Selected Lines ◽  
Recurrent Parent Genome ◽  
Backcross Breeding ◽  
Marker Assisted Backcross Breeding

The most vital aspect of marker-assisted backcross breeding is the recurrent parent genome recovery. This enables the selection of only parents with recovered recipient/recurrent parent genome in addition to the targeted genes. The recurrent parent genome recovery (RPGR) ensures that non-desirable genomic segments are removed while the gene of interest is sustained in the recombined progenies without further segregations. This work was aimed at quantifying the RPGR of backcross populations with introgression of bacterial leaf blight resistance genes. Putra-1, a Malaysian elite variety, high yielding with inherent resistance to blast but susceptible to bacterial leaf blight (BLB), was crossed with IRBB60 which is resistant to BLB disease. The IRBB60 has four Xoo resistance genes—Xa4, xa5, xa13 and Xa21. Tightly linked polymorphic functional and SSR markers were used for foreground selection at every stage of backcrossing to select progenies with introgressed target genes. Background selection was done to quantify the percentage of RPGR in the selected lines using 79 confirmed polymorphic microsatellites. Result obtained showed that the percentage of RPGR was 80.11% at BC1F1, 95.30% at BC2F1 and 95.9% at BC2F2. The introgression of Xa4, xa5, xa13 and Xa21 Xoo resistance genes were faster through the adopted marker-assisted backcross breeding compared to what could be obtained through conventional breeding. All the 16 selected lines displayed resistance to BLB with three lines showing high resistance to the disease. The blast resistance contained in the genetic background of Putra-1 was also sustained in all the selected lines. The newly developed lines were recommended as new rice varieties for commercial cultivation.

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