scholarly journals Morphological and molecular identification of Cytospora chrysosperma causing canker disease on Prunus persica

2017 ◽  
Vol 12 (1) ◽  
Author(s):  
Shima Bagherabadi ◽  
Doustmorad Zafari ◽  
Mohammad Javad Soleimani
Keyword(s):  
Molecular Identification ◽  
Prunus Persica ◽  
Canker Disease ◽  
Cytospora Chrysosperma

Leaf abscission kinetics of peach cultivars and clones in relation to peach canker disease

1991 ◽  
Vol 69 (9) ◽  
pp. 2020-2025 ◽  
Author(s):  
A. R. Biggs
Keyword(s):  
Prunus Persica ◽  
Field Performance ◽  
Wound Response ◽  
Leaf Abscission ◽  
Canker Disease ◽  
Progress Curve ◽  
Cultivar Susceptibility ◽  
Kinetics Of ◽  
Canker Pathogens

Leaf abscission kinetics of several peach cultivars and clones were studied for 2 or 3 years at two locations. Cultivars varied significantly in leaf abscission rates, dates of 50 and 75% leaf abscission, and area under the abscission progress curve. ‘Veeglo’, ‘Madison’, and ‘Vivid’ exhibited the most rapid rates of leaf abscission, clone V68101 and ‘Redhaven’ showed moderate rates of leaf abscission, and ‘Babygold 5’, ‘Vanity’, ‘Candor’, ‘Earlired’, and clone V68051 were relatively slow to abscise leaves. No significant relationship could be demonstrated for any of the leaf abscission parameters with field performance ranks of cultivar susceptibility to the peach canker pathogens. Mean annual harvest date for the various cultivars or clones was not associated with susceptibility to the peach canker pathogens or any of the leaf abscission parameters. The role of autumn wound response in relation to screening for pathogen resistant peach genotypes is discussed. Key words: Prunus persica, Leucostoma spp., defoliation, resistance.

scholarly journals Kanker Batang: Penyakit Baru pada Kopi di Lampung

2019 ◽  
Vol 15 (1) ◽  
pp. 9
Author(s):  
Suryo Wiyono ◽  
Andika Septiana Suryaningsih ◽  
Ali Wafa ◽  
Efi Toding Tondok ◽  
Bonjok Istiaji ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Fusarium Solani ◽  
Stem Canker ◽  
New Disease ◽  
Coffee Plantations ◽  
Canker Disease ◽  
Coffee Plants ◽  
Colony Shape ◽  
Canker Pathogens ◽  
Shape And Size

Stem Canker: A New Disease of coffee in LampungStem cancer is a new disease that has attacked smallholder coffee plantations in Lampung since 2010. The cause of the disease was unknown. This study aims to describe the symptoms of the disease, the incidence of the disease in the affected plantation, and identify morphologically and molecularly the canker pathogens of the coffee stem canker diseases. All stages of Koch’s postulate were carried out in laboratories and greenhouses. The isolated pathogens were morphologically characterized by colony shape and color as well as the conidia shape and size. Molecular identification was carried out by using a general primer (ITS1 and ITS4) and followed by sequencing. The main symptoms of the disease are stem cancer and dieback, as well as more infecting older plants. Pathogen of the coffee stem canker disease that attacks coffee plants in Lampung has been identified as Fusarium solani which has 99% homology with F. solani KY245947.1.

scholarly journals Morphological and Molecular Identification of Fungi Causing Canker Disease on Melia Azidarch Trees in Some Regions of Mosul Provinces, Iraq

2021 ◽  
Vol 910 (1) ◽  
pp. 012116
Author(s):  
Anwer Noori Alkhero ◽  
Zainab Waadallah Rassem
Keyword(s):  
Molecular Diagnosis ◽  
Molecular Identification ◽  
First Record ◽  
Alternaria Brassicicola ◽  
Morphological Identification ◽  
University Campus ◽  
Canker Disease ◽  
Identification Of Fungi ◽  
Nattrassia Mangiferae ◽  
High Pathogenic

Abstract The aim of this study was to identify the fungi associated with canker disease on Melia azidarch trees inside Mosul University campus and the presidential palaces regions in Mosul Province, Iraq. Results of isolation showed the presenting of the fungi (Nattrassia mangiferae, Neoscylitidium dimidiatum Penz., Fusarium graminearium Schw., Alternaria brassicicola Schw., Aspergillus sp. and Penecillium sp.), which accompanied with the samples displayed cankering symptoms during the period from April to December/2020, the maximum of dominance was 85% for the fungus Neoscylitidium dimidiatum in August, while the lowest was 49% in April for the same year, followed by Fusarium graminearium with 38% in December, while the lowest percentage was 4% in October, then Alternaria brassicicola Schw. was 25% in April and the lowest value was 0% in August, followed by Aspergillus sp. and Penecillium sp. with low isolation percentages the maximum of which 25% and the lowest is 0% in August. When studying the pathogenicity of the isolated fungi, the results showed a high pathogenic effect in terms the length, diameter and the area of cankers symptom. Based on the results of the molecular diagnosis, the morphological identification was confirmed and it was clear that Fusarium austroamericanum, detection is considered the first record of this fungus in Iraq Melia azidarch trees.

scholarly journals First Report of Phomopsis amygdali Causing Fruit Rot on Peaches in Greece

Plant Disease ◽  
2006 ◽  
Vol 90 (12) ◽  
pp. 1551-1551 ◽  
Author(s):  
T. J. Michailides ◽  
T. Thomidis
Keyword(s):  
Prunus Persica ◽  
Morphological Characteristics ◽  
Fruit Rot ◽  
Peach Fruit ◽  
Koch’S Postulates ◽  
Canker Disease ◽  
First Report ◽  
Immature Fruit ◽  
Diseased Fruit ◽  
Koch's Postulates

In the summer of 2005, the fungus Phomopsis amygdali (Del.) Tuset & Portilla was frequently isolated from decayed peaches (Prunus persica cv. Andross) grown in the province of Imathia, Greece. Fruit infected by P. amygdali developed gray-to-brown decay lesions with white mycelium forming on the surface of lesions. Identification of the pathogen was based on morphological characteristics. Dark-pigmented pycnidia (flask-shaped, conidia-bearing fruiting bodies) were produced over the surface of potato dextrose agar. The pycnidia exuded conidia in white tendrils 7 days later. Koch's postulates were completed in the laboratory by inoculating mature and immature cv. Andross peach fruits with an isolate of P. amygdali isolated from decayed cv. Andross peaches. Thirty peach fruit were surface sterilized by dipping them into 0.1% chlorine solution and allowing them to dry in a laminar flow hood. The peach fruit were wounded with a 2-mm diameter glass rod and a 40-μl drop of 5 × 105 conidia of P. amygdali per milliliter suspension was applied to the wound. Thirty control fruits were similarly wounded and inoculated with a 40-μl drop of sterile water. All inoculated and noninoculated fruit were incubated at 24 to 26°C for 7 days. Koch's postulates were satisfied when the same fungus was reisolated from 100% of inoculated mature and immature fruit that developed symptoms similar to diseased fruit collected from orchards. Although P. amygdali has been previously reported as a causal agent of canker disease (2) and fruit rots of peaches (1) in other countries, to our knowledge, this is the first report of the occurrence of P. amygdali causing a fruit rot of peaches in Greece. References: (1) Y. Ko and S. Sun. Plant Pathol. Bull. 12:212, 2003. (2) E. I. Zehr, Constriction canker. Page 31 in: Compendium of Stone Fruit Diseases. J. M. Ogawa et al., eds. The American Phytopathological Society, St. Paul, MN, 1995.

CYTOSPORA CANKER OF POPLARS: THE MOISTURE RELATIONS AND ANATOMY OF THE HOST

1962 ◽  
Vol 40 (10) ◽  
pp. 1281-1292 ◽  
Author(s):  
W. J. Bloomberg
Keyword(s):  
Moisture Content ◽  
Populus Trichocarpa ◽  
Sieve Tube ◽  
Moisture Loss ◽  
Basal Region ◽  
Wood Moisture ◽  
Canker Disease ◽  
Moisture Contents ◽  
Cytospora Chrysosperma ◽  
Two Hybrid

A comparison was made between Populus trichocarpa Torr. and Gray, P. × canadensis Moench 'Regenerata', and P. × canadensis 'Robusta Bachelieri' with respect to shoot moisture relations and anatomy. The two hybrid varieties had slower rates of moisture loss and had higher wood moisture contents throughout most of the year than P. trichocarpa. The bark and wood moisture content of the three varieties was lower during dormancy than in the growing season. The upper region of the shoots had lower bark and wood moisture contents than the basal region during dormancy, but the reverse was true in summer. In all varieties, there was a significant positive correlation between wood and bark moisture content. Compared with P. trichocarpa, the two hybrids had larger piths, wider vessels, longer phloem rays, wider sieve tube zones, and thicker periderms; P. 'Robusta' had fewer lenticels. The upper region of the shoots had a wider pith, thicker bark, thinner periderm, and more lenticels than the basal region. The differences in moisture relations and anatomy of the three varieties suggest an explanation for the observed greater resistance to canker disease caused by Cytospora chrysosperma (Pers.) Fr. in the hybrids than in P. trichocarpa.

scholarly journals Studies on Diaporthe eres as a New Pathogen of Peach Trees in Greece

Plant Disease ◽  
2009 ◽  
Vol 93 (12) ◽  
pp. 1293-1297 ◽  
Author(s):  
Thomas Thomidis ◽  
Themis J. Michailides
Keyword(s):  
Prunus Persica ◽  
Fruit Rot ◽  
Disease Symptoms ◽  
Canker Disease ◽  
Thiophanate Methyl ◽  
C Storage ◽  
Shoot Blight ◽  
Pathogenicity Tests ◽  
Peach Trees

Phomopsis sp., the imperfect stage of the genus Diaporthe, is an increasingly common fungal pathogen of peach (Prunus persica). This study is the first report of the occurrence of a shoot blight and canker disease of peach in Greece caused by the fungus Diaporthe eres. The pathogen caused distinct cankers with abundant gumming on shoots of peach and nectarine trees. The rate of development of D. eres in vitro was reduced as temperatures increased from 25 to 30°C or decreased from 25 to 15°C, and was totally inhibited at 35 and 10°C. Storage at 10°C of peach fruit inoculated with D. eres controlled the development of fruit rot. Pathogenicity tests showed that 27 peach and nectarine cultivars grown in Imathia Prefecture, Greece, were equally susceptible to D. eres. The fungicides thiophanate methyl, carbendazim, tebuconazole, iprodione, and the mixture of cyprodinil:fludioxonil were evaluated against the development of D. eres and the disease symptoms. Thiophanate methyl, carbendazim, and tebuconazole significantly inhibited the growth of D. eres whereas iprodione and the mixture of cyprodinil:fludioxonil were less effective in inhibiting mycelial growth and disease symptoms. The sensitivity of 24 isolates of D. eres to carbendazim, thiophanate methyl, and tebuconazole was also tested. The results showed that most of the isolates used were sensitive to these fungicides, with some isolates showing a level of insensitivity. In general, the disease caused by D. eres could be a threat for peach cultivation in Greece and its management should be investigated in the field.

Electron microscopy in diagnostic virology

1984 ◽  
Vol 42 ◽  
pp. 70-73
Author(s):  
S. E. Miller
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Molecular Identification ◽  
Point Of View ◽  
Negative Stain ◽  
Viral Diagnosis ◽  
Electron Microscopist ◽  
Diagnostic Virology

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.

Variedades de durazno (Prunus persica L.) intercaladas en el sistema milpa en la sierra nevada de Puebla, México

2019 ◽  
Vol 12 (9) ◽  
Author(s):  
Ernesto Hernández-Romero ◽  
Reyna Rojano-Hernández ◽  
Ricardo Mendoza-Robles ◽  
José. I. Cortés- Flores ◽  
Antonio N. Turrent-Fernández
Keyword(s):  
Sierra Nevada ◽  
Prunus Persica ◽  
El Sistema

En la Sierra Nevada de Puebla, México, los huertos de durazno (Prunus persica L.) presentan problemas de producción relacionados con alta incidencia de plagas (incluye enfermedades), nutrición deficiente e inadecuado manejo de poda, que acentúan el problema de floración precoz en la mayoría de las variedades mejoradas.

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