In vivo and invitro experimentation for scientific validation of the traditional use of Chromolaena odorata (L.) against envenomation of honey bee sting

2021 ◽  
Author(s):  
Tania S. Das ◽  
Biswadeep B. Das ◽  
Chandrasekhar Mote
Keyword(s):  
Honey Bee ◽  
Chromolaena Odorata ◽  
Traditional Use ◽  
Bee Sting ◽  
Scientific Validation

scholarly journals Efficacy of Nyagrodh Twak Lepa in Honey bee sting: In-vivo study

2021 ◽  
Vol 12 (4) ◽  
pp. 800-806
Author(s):  
Amrit Malik ◽  
Chinky Goyal ◽  
Abhiram S P ◽  
Gramopadhye N G
Keyword(s):  
Honey Bee ◽  
Apis Cerana ◽  
In Vivo Study ◽  
Control Group ◽  
Preliminary Treatment ◽  
Standard Drug ◽  
Bee Sting ◽  
Albino Mice ◽  
Trial Drug

Introduction- As Acharya Charaka has explained the local application of Kshirivruksha Twak to cure all types of keeta visha, hence Nyagrodh (Ficus benghalensis L.) Twak Lepa with water as base is selected as Trial drug on Apis Cerana Indica bee sting poisoning. Material and Methods- An in-vivo study on albino mice to know the efficacy of trial drug has been planned after animal ethical clearance. 18 albino mice were prorated into three groups with 6 animals in each group viz. Control group, Trial drug (Nyagrodh Twak Lepa Churna) group and Standard drug (Beclomethasone Dipropionate 0.025% w/w) group. 6 stings were given to each mice and 3 stings were removed after sting operation. All mice were observed for allergic reactions viz. erythema, scaling, fissures, oedema and mortality for a period of 7 days. Histo-pathological changes were also noted after completion of study. Statistical analysis was done using Paired t test. Results- Results revealed that Trial drug had worked more efficiently on Erythema and Oedema while Standard drug worked more efficiently on Scaling and Fissure. Histo-pathology showed that wound healed with Nyagrodh twak lepa and Standard drug have shown almost similar changes while wound in control group showed extensive areas of necrosis. Conclusion- Present study suggests that both Nyagrodh and Beclomethasone can be used in Honey bee sting poisoning but as Nyagrodh being a religious tree can be easily identified by a common man, it can be employed as preliminary treatment for the same before reaching hospital. 

Ethnomedicinal uses, Phytochemistry and Pharmacological Aspects of the Genus Berberis Linn: A Comprehensive Review

2020 ◽  
Vol 23 ◽  
Author(s):  
Roohi Mohi-ud-din ◽  
Reyaz Hassan Mir ◽  
Prince Ahad Mir ◽  
Saeema Farooq ◽  
Syed Naiem Raza ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Pharmacological Activities ◽  
Traditional Use ◽  
Comprehensive Review ◽  
Wide Range ◽  
Anti Cancer ◽  
Comprehensive Survey ◽  
Ethnomedicinal Uses

Background: Genus Berberis (family Berberidaceae), which contains about 650 species and 17 genera worldwide, has been used in folklore and various traditional medicine systems. Berberis Linn. is the most established group among genera with around 450-500 species across the world. This comprehensive review will not only help researchers for further evaluation but also provide substantial information for future exploitation of species to develop novel herbal formulations. Objective: The present review is focussed to summarize and collect the updated review of information of Genus Berberis species reported to date regarding their ethnomedicinal information, chemical constituents, traditional/folklore use, and reported pharmacological activities on more than 40 species of Berberis. Conclusion: A comprehensive survey of the literature reveals that various species of the genus possess various phytoconstituents mainly alkaloids, flavonoid based compounds isolated from different parts of a plant with a wide range of pharmacological activities. So far, many pharmacological activities like anti-cancer, anti-hyperlipidemic, hepatoprotective, immunomodulatory, anti-inflammatory both in vitro & in vivo and clinical study of different extracts/isolated compounds of different species of Berberis have been reported, proving their importance as a medicinal plant and claiming their traditional use.

Evaluation of Anti-diabetic and Anti-hyperlipidemic Activity of Isolated Bioactive Compounds of Leaves of Annona reticulata Linn.

2020 ◽  
Vol 10 ◽  
Author(s):  
Kalyani Pathak ◽  
Aparoop Das ◽  
Anshul Shakya ◽  
Riya Saikia ◽  
Himangshu Sarma
Keyword(s):  
Spectral Analysis ◽  
Gallic Acid ◽  
Liver Enzymes ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Traditional Use ◽  
Per Oral ◽  
Diabetes And Obesity ◽  
Annona Reticulata

Background: The leaves of Annona reticulata Linn. have been traditionally used by the tribes of Assam as a source of medicine to mitigate a range of health ailments including diabetes and obesity. Objectives: The current study aimed to evaluate the anti-diabetic and anti-hyperlipidemic potential of bioactive fractions isolated from the methanolic extract of Annona reticulata Linn. leaves using Nicotinamide + Streptozotocin (60 mg/kg, i.p.) induced diabetic rats. Methods: The partially purified bioactive fractions, namely F1, F2, F3 and F4 were administered to diabetic rats with the dose of 200 mg/kg, per oral (p.o.) and the effect of the fractions on serum glucose were studied up to 21 days. The potent fractions were further subjected for spectral analysis for identification of the isolated active compounds. Results: The in-vivo anti-diabetic activity of the isolated fractions F2 and F3 were found significant controlling blood glucose level, alike glibenclamide. Interestingly, F2 and F3 treated animals were found significant in restoring the lipid and liver enzymes profile in streptozotocin challenge rats. Further, spectral analysis revealed that F2 and F3 were comprises Quercetin and Gallic acid, respectively. Conclusion: Outcome of finding demonstrate the anti-diabetic and anti-hyperlipidemic potential of the isolates/fractions of A. reticulata, which were found enriched in polyphenolics including Quercetin and Gallic acid; and provides logistic behind the traditional use of the A. reticulata against Diabetes and obesity.

scholarly journals A RARE CASE OF HEMIPLEGIA

2020 ◽  
pp. 30-30
Author(s):  
Valeti Rajeswari ◽  
Kolluru V D Karthik ◽  
Srinivasula Sriranga Pravallika
Keyword(s):  
Cerebral Infarction ◽  
Honey Bee ◽  
Anaphylactic Shock ◽  
Rare Case ◽  
The Body ◽  
Intravenous Fluids ◽  
Rare Entity ◽  
Bee Sting ◽  
The Face

Honey bee sting induced Cerebral infarction is a rare entity . We report a case of 55year old male presented with anaphylactic shock following honey bee sting along with weakness of left side of the body and the face . He was managed with anti histaminics, adrenaline injections, Intravenous fluids , vasopressors , anti platelets and anticoagulants .

scholarly journals Morphology of the Dufour gland within the honey bee sting gland complex

Apidologie ◽  
2005 ◽  
Vol 36 (4) ◽  
pp. 543-546 ◽  
Author(s):  
Stephen J. Martin ◽  
Vicky Dils ◽  
Johan Billen
Keyword(s):  
Honey Bee ◽  
Dufour Gland ◽  
Bee Sting

scholarly journals Comparative testing of different methods for evaluation of Varroa destructor infestation of honey bee colonies

2011 ◽  
Vol 43 (3) ◽  
pp. 323
Author(s):  
Nikolay D. Dobrynin ◽  
Mario Colombo ◽  
Francesca Romana Eördegh
Keyword(s):  
Honey Bee ◽  
Varroa Destructor ◽  
Comparative Testing ◽  
In Vivo Evaluation ◽  
Bee Colonies

Different methods for evaluation of the degree of Varroa destructor infestation of honey bee colonies were tested. The methods using in vivo evaluation were the most sparing for the bees but less precise. The methods using evaluation with the killing of the bees or brood were the most precise but less sparing for bees.

In vivo validation of in vitro quality tests for cryopreserved honey bee semen

Cryobiology ◽  
2012 ◽  
Vol 65 (2) ◽  
pp. 126-131 ◽  
Author(s):  
Jakob Wegener ◽  
Tanja May ◽  
Ulrich Knollmann ◽  
Günter Kamp ◽  
Karin Müller ◽  
...  
Keyword(s):  
Honey Bee ◽  
In Vivo Validation

Cytochemical studies on the poison gland of honey-bee sting

1972 ◽  
Vol 82 (1) ◽  
pp. 91-96 ◽  
Author(s):  
S. Grzycki ◽  
K. Czerny
Keyword(s):  
Honey Bee ◽  
Poison Gland ◽  
Bee Sting

scholarly journals In VitroandIn VivoAntimalarial Evaluations of Myrtle Extract, a Plant Traditionally Used for Treatment of Parasitic Disorders

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Farzaneh Naghibi ◽  
Somayeh Esmaeili ◽  
Noor Rain Abdullah ◽  
Mehdi Nateghpour ◽  
Mahdieh Taghvai ◽  
...  
Keyword(s):  
Cell Lines ◽  
Mammalian Cell ◽  
Methanolic Extract ◽  
Cytotoxic Activities ◽  
Traditional Use ◽  
Aerial Parts ◽  
Mammalian Cell Lines ◽  
Phytochemical Investigation

Based on the collected ethnobotanical data from the Traditional Medicine and Materia Medica Research Center (TMRC), Iran,Myrtus communisL. (myrtle) was selected for the assessment ofin vitroandin vivoantimalarial and cytotoxic activities. Methanolic extract of myrtle was prepared from the aerial parts and assessed for antiplasmodial activity, using the parasite lactate dehydrogenase (pLDH) assay against chloroquine-resistant (K1) and chloroquine-sensitive (3D7) strains ofPlasmodium falciparum. The 4-day suppressive test was employed to determine the parasitemia suppression of the myrtle extract againstP. berghei  in vivo. The IC50values of myrtle extract were 35.44 µg/ml against K1 and 0.87 µg/ml against 3D7. Myrtle extract showed a significant suppression of parasitaemia (84.8 ± 1.1% at 10 mg/kg/day) in mice infected withP. bergheiafter 4 days of treatment. Cytotoxic activity was carried out against mammalian cell lines using methyl thiazol tetrazolium (MTT) assay. No cytotoxic effect on mammalian cell lines up to 100 µg/mL was shown. The results support the traditional use of myrtle in malaria. Phytochemical investigation and understanding the mechanism of action would be in our upcoming project.

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