Determination of Phytochemical Contents of Avena sativa (oat) and Its Impact on Debaryomyces hansenii

2013 ◽  
Vol 84 (2) ◽  
pp. 365-371 ◽  
Author(s):  
Pınar Erecevıt ◽  
Sevda Kırbağ ◽  
Fikriye Zengin
Keyword(s):  
Avena Sativa ◽  
Debaryomyces Hansenii ◽  
Phytochemical Contents

scholarly journals Genetic Similarity of Avena sativa L. Varieties as an Example of a Narrow Genetic Pool of Contemporary Cereal Species

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1424
Author(s):  
Magdalena Cieplak ◽  
Sylwia Okoń ◽  
Krystyna Werwińska
Keyword(s):  
Genetic Diversity ◽  
Avena Sativa ◽  
Genetic Similarity ◽  
Breeding Programs ◽  
Central European ◽  
Cereal Species ◽  
Genetic Pool ◽  
Selection Of ◽  
High Genetic Similarity

The assessment of the genetic diversity of cultivated varieties is a very important element of breeding programs. This allows the determination of the level of genetic differentiation of cultivated varieties, their genetic distinctiveness, and is also of great importance in the selection of parental components for crossbreeding. The aim of the present study was to determine the level of genetic diversity of oat varieties currently grown in Central Europe based on two marker systems: ISSR and SCoT. The research conducted showed that both these types of markers were suitable for conducting analyses relating to the assessment of genetic diversity. The calculated coefficients showed that the analyzed cultivars were characterized by a high genetic similarity. However, the UPGMA and PCoA analyses clearly indicated the distinctiveness of the breeding programs conducted in Central European countries. The high genetic similarity of the analyzed forms allow us to conclude that it is necessary to expand the genetic pool of oat varieties. Numerous studies show that landraces may be the donor of genetic variation.

scholarly journals Qualitative and Quantitative Determination of Phytochemical Contents of Indigenous Nigerian Softwoods

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Chukwuma S. Ezeonu ◽  
Chigozie M. Ejikeme
Keyword(s):  
Alternative Medicine ◽  
Moringa Oleifera ◽  
Industrial Applications ◽  
Qualitative And Quantitative Analysis ◽  
Dichrostachys Cinerea ◽  
Qualitative And Quantitative ◽  
Kaempferia Galanga ◽  
Anogeissus Leiocarpus ◽  
Phytochemical Contents

The phytochemical contents of some milled Nigerian softwood chips were carried out in a quest to evaluate their potentials as sources of alternative medicine as well as uses in other industrial applications. The qualitative and quantitative analysis were ascertained. Tannin was found in all the Nigerian softwoods examined with the highest quantities obtained in Sterculia oblonga (1240 mg/100 g) and Barteria nigritiana (1230 mg/100 g). Highest quantities of alkaloid were obtained in Cordia millenii (11.2%) and Sterculia oblonga (10.4%). Barteria nigritiana (14.2%) and Moringa oleifera (12.2%) recorded more flavonoid content than other individual softwoods. Saponin was more in Anogeissus leiocarpus (12.5%) and Dichrostachys cinerea (9.8%). Oxalate was found to be higher in Combretodendron macrocarpum (5.84 g/100 g) and Glyphaea brevis (3.55 g/100 g). Pentaclethra macrophylla (890 mg/100 g) and Moringa oleifera (880 mg/100 g) contained more cyanogenic glycosides. Sacoglottis gabonensis (4.68 mg/g) and Pentaclethra macrophylla (4.04 mg/g) showed the highest contents of phenol, while more lipids (8% and 7.2%) were found in Anogeissus leiocarpus and Kaempferia galanga, respectively. The results showed that these Nigerian softwoods grains could be a source for the exploitation of these phytochemicals beneficial in the pharmaceutical and alternative medicine industries.

scholarly journals Debaryomyces hansenii Strains Isolated From Danish Cheese Brines Act as Biocontrol Agents to Inhibit Germination and Growth of Contaminating Molds

2021 ◽  
Vol 12 ◽  
Author(s):  
Chuchu Huang ◽  
Ling Zhang ◽  
Pernille Greve Johansen ◽  
Mikael Agerlin Petersen ◽  
Nils Arneborg ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Debaryomyces Hansenii ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromosome Polymorphism ◽  
Species Variation ◽  
Inhibitory Effects ◽  
Mycelium Growth ◽  
Native Strains ◽  
Germination And Growth

The antagonistic activities of native Debaryomyces hansenii strains isolated from Danish cheese brines were evaluated against contaminating molds in the dairy industry. Determination of chromosome polymorphism by use of pulsed-field gel electrophoresis (PFGE) revealed a huge genetic heterogeneity among the D. hansenii strains, which was reflected in intra-species variation at the phenotypic level. 11 D. hansenii strains were tested for their ability to inhibit germination and growth of contaminating molds, frequently occurring at Danish dairies, i.e., Cladosporium inversicolor, Cladosporium sinuosum, Fusarium avenaceum, Mucor racemosus, and Penicillium roqueforti. Especially the germination of C. inversicolor and P. roqueforti was significantly inhibited by cell-free supernatants of all D. hansenii strains. The underlying factors behind the inhibitory effects of the D. hansenii cell-free supernatants were investigated. Based on dynamic headspace sampling followed by gas chromatography-mass spectrometry (DHS-GC-MS), 71 volatile compounds (VOCs) produced by the D. hansenii strains were identified, including 6 acids, 22 alcohols, 15 aldehydes, 3 benzene derivatives, 8 esters, 3 heterocyclic compounds, 12 ketones, and 2 phenols. Among the 71 identified VOCs, inhibition of germination of C. inversicolor correlated strongly with three VOCs, i.e., 3-methylbutanoic acid, 2-pentanone as well as acetic acid. For P. roqueforti, two VOCs correlated with inhibition of germination, i.e., acetone and 2-phenylethanol, of which the latter also correlated strongly with inhibition of mycelium growth. Low half-maximal inhibitory concentrations (IC50) were especially observed for 3-methylbutanoic acid, i.e., 6.32–9.53 × 10–5 and 2.00–2.67 × 10–4 mol/L for C. inversicolor and P. roqueforti, respectively. For 2-phenylethanol, a well-known quorum sensing molecule, the IC50 was 1.99–7.49 × 10–3 and 1.73–3.45 × 10–3 mol/L for C. inversicolor and P. roqueforti, respectively. For acetic acid, the IC50 was 1.35–2.47 × 10–3 and 1.19–2.80 × 10–3 mol/L for C. inversicolor and P. roqueforti, respectively. Finally, relative weak inhibition was observed for 2-pentanone and acetone. The current study shows that native strains of D. hansenii isolated from Danish brines have antagonistic effects against specific contaminating molds and points to the development of D. hansenii strains as bioprotective cultures, targeting cheese brines and cheese surfaces.

Comparison of Chlorophyll Fluorescence and Fresh Weight as Herbicide Bioassay Techniques

Weed Science ◽  
1985 ◽  
Vol 33 (1) ◽  
pp. 29-33 ◽  
Author(s):  
David R. Shaw ◽  
Thomas F. Peeper ◽  
D. L. Nofziger
Keyword(s):  
Chlorophyll Fluorescence ◽  
Avena Sativa ◽  
Inflection Point ◽  
Plant Size ◽  
Variable Fluorescence ◽  
Fresh Weight ◽  
Reliable Technique ◽  
Treated Soil ◽  
Fluorescence Maximum

The sensitivities of chlorophyll fluorescence and fresh weight as bioassay techniques for the determination of metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5 (4H)-one], diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea], and atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine] concentrations in soil were compared. The ratio of the initial inflection point (I) to the variable fluorescence maximum (P) of intact oat (Avena sativaL. ‘Chilocco’) leaves was determined for plants seeded directly into herbicide-treated soil and for those transplanted into treated soil after 14 days of growth in nutrient solution. Using the chlorophyll fluorescence of transplanted oats bioassay, 0-, 0.13-, 0.25-, and 0.50-ppm concentrations could be distinguished from one another within 8 h for metribuzin, within 24 h for diuron, and within 48 h for atrazine. These distinctions between rates could not be made 17 days after seeding into treated soil when using fresh weight as the bioassay indicator. Chlorophyll fluorescence of oats seeded directly into treated soil was also a reliable technique, but required much more time to attain sufficient plant size for convenient chlorophyll fluorescence determinations.

Colorimetric determination of aconitic acid in Avena sativa (oat)

1970 ◽  
Vol 18 (3) ◽  
pp. 546-547 ◽  
Author(s):  
William E. Poe ◽  
Ben F. Barrentine
Keyword(s):  
Avena Sativa ◽  
Colorimetric Determination ◽  
Aconitic Acid

scholarly journals Comparative study of yield, intake, chemical composition and nutritive values of gama (Avena sativa) and sorghum (Sorghum bicolor) forages

1970 ◽  
Vol 39 (1-2) ◽  
pp. 67-74
Author(s):  
MN Islam ◽  
MR Alam ◽  
AKMA Kabir ◽  
NG Das ◽  
MM Khanduker
Keyword(s):  
Chemical Composition ◽  
Sorghum Bicolor ◽  
Avena Sativa ◽  
Latin Square ◽  
Nutrient Digestibility ◽  
Tripsacum Dactyloides ◽  
Indigenous Sheep ◽  
Nutritive Values ◽  
Better Than

An experiment was conducted to compare the yield, composition and nutrient digestibility of gama (Tripsacum dactyloides), oat (Avena sativa) and sorghum (Sorghum bicolor) forages. Three fodders were cultivated and a metabolism trial was conducted with 3 indigenous sheep in a 3 × 3 Latin Square Design (LSD) for determination of nutrient digestibility. The fresh yield of gama was higher (P<0.05) than that of oat and sorghum. The yield of DM in gama and sorghum was similar and significantly higher (P<0.01) than oat. No significant differences (P>0.05) were observed in DM, OM, CP, NDF and ADF contents among the gama, oat and sorghum forages. The average DM and OM intake in sheep fed sorghum was higher (P<0.05) than that of oat or gama. Sheep fed sorghum or oat showed significantly (P<0.01) higher CP intake than gama. Average NDF and ADF intake was higher (P<0.05) in sheep fed sorghum followed by oat or gama, respectively. The DM digestibility of different forages was found non-significant (P>0.05). The OM digestibility of sorghum was found to be higher (P<0.05) compared to oat or gama. Digestibility of CP was significant (P<0.01) higher sorghum than oat (37 %) or gama (36 %). Higher NDF and ADF digestibility (P<0.05) were found in sorghum and oat, respectively than gama. It was concluded that sorghum (Sorghum bicolor) is better than gama (Tripsacum dactyloides) and oat (Avena sativa) forages. Key words: Gama; Oat; Sorghum; Digestibility DOI: http://dx.doi.org/10.3329/bjas.v39i1-2.9678 Bang. J. Anim. Sci. 2010, 39(1&2): 67-74

scholarly journals Comparative Quantitative Study on Phytochemical Contents and Antioxidant Activities of Capsicum annuum L. and Capsicum frutescens L.

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Tomi Lois Olatunji ◽  
Anthony Jide Afolayan
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Group Method ◽  
Phytochemical Content ◽  
Additional Tool ◽  
Pair Group ◽  
Effective Selection ◽  
The Relationship ◽  
Phytochemical Contents

The variabilities in the phytochemical contents and antioxidant activities of four varieties of two closely related cultivated Capsicum species, C. annuum and C. frutescens, were examined as an additional tool for establishing their phylogenetic relatedness and for breeding purposes. The methodology involved the use of ethanolic and aqueous extractions for the determination of the phytochemical and antioxidant properties. The phytochemical contents including total flavonoid, total phenol, and proanthocyanidins were evaluated spectrophotometrically while the antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), nitric oxide (NO), and phosphomolybdenum assays. To point out the relationship among the varieties, a dendrogram based on the antioxidative phytochemical contents was constructed using the unweighted pair group method with arithmetic mean (UPMGA) cluster analysis. In all, aqueous extracts gave higher yield while ethanolic extracts showed higher phytochemical content across the varieties. Significant variations were observed among the varieties in relation to their phytochemical constituents and antioxidant activities. Dendrogram obtained from multivariate analysis distinguished the two Capsicum species. The first cluster contained only C. frutescens var. baccatum while the second cluster contained the three varieties of C. annuum species in subclusters, signifying the close genetic affinity among the three varieties. It also revealed that the four varieties are of a common progenitor. Information from this study gives additional evidence of chemotaxonomic significance and baseline data for effective selection of suitable parental genotypes in breeding for nutritional and pharmacological purposes.

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