Preliminary in vitro tests on inhibitory activity of distinct plant extracts toward bacterial pathogens of fruit and nut trees

2021 ◽  
Author(s):  
Monika Kałużna ◽  
Piotr Sobiczewski ◽  
Ana M. Dobreva ◽  
Ganka S. Baeva ◽  
Anatoli G. Dzurmanski ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Inhibitory Activity ◽  
Bacterial Pathogens ◽  
In Vitro Tests

scholarly journals Synthesis and AChE Inhibitory Activity of Novel Thiazolylhydrazone Derivatives

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2392 ◽  
Author(s):  
Derya Osmaniye ◽  
Begüm Nurpelin Sağlık ◽  
Ulviye Acar Çevik ◽  
Serkan Levent ◽  
Betül Kaya Çavuşoğlu ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Inhibitory Activity ◽  
New Drugs ◽  
Brain Diseases ◽  
Structure Identification ◽  
Ache Inhibitor ◽  
In Vitro Tests ◽  
Active Inhibitor

Alzheimer’s disease (AD) is the most common of the degenerative brain diseases and is described together with the impairment of cognitive function. Patients with AD lose the capability to code new memories, and life conditions are extremely difficult. The development of new drugs in this area continues at a great pace. A novel series of thiazole-piperazine hybrids, aimed against Alzheimer’s disease (AD), have been synthesized. The structure identification of synthesized compounds was elucidated by 1HNMR, 13C-NMR, and LCMSMS spectroscopic methods. The inhibitory potential of the synthesized compounds on cholinesterase enzymes was investigated. The compounds 3a, 3c and 3i showed significant inhibitory activity on the acetylcholinesterase (AChE) enzyme. On the other hand, none of the compounds showed significant inhibitory activity on the butyrylcholinesterase (BChE) enzyme. In addition to enzyme inhibition studies, enzyme kinetic studies were performed to observe the effects of the most active inhibitor compounds on the substrate–enzyme relationship. In addition to in vitro tests, docking studies also indicated that compound 3c potentially acts as a dual binding site AChE inhibitor.

scholarly journals In vitro evaluation of the antimicrobial activity of Basil (Ocimum baslicum L.) and Coriander (Coriandrum satirum L.) oil extracts on Streptococus mutans

2018 ◽  
Vol 5 (2) ◽  
pp. 40 ◽  
Author(s):  
Mariana Lourenço ◽  
Adilson Cesar Abreu Bernardi ◽  
Nadia Lunardi ◽  
Rodolfo Jorge Boeck Neto ◽  
Patricia Sigilló Mazzoni Bernardi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Inhibitory Concentration ◽  
Growth Control ◽  
Ocimum Basilicum ◽  
Coriandrum Sativum ◽  
Microdilution Method

Plant extracts and essential oils show efficiency on growth control in a wide variation of microorganisms, including filamentous fungi, yeasts and bacteria. To evaluate antimicrobial activity of plant extracts, determine the lower quantity of substance to inhibit the microorganism test growth is necessary. This value is known as Minimum Inhibitory Concentration (MIC). This study had as aim to verify the antimicrobial action and the Minimum Inhibitory Concentration (MIC) of basil (Ocimum basilicum L.) and coriander (Coriandrum sativum L.) oil extract before S. mutans (ATCC 25175) strains. Antimicrobial activity determination was carried out by microdilution method and performed according to recommendations of CLSI (previously known as NCCLS), standard M7-A6 (NCCLS, 2003) for bacteria, and standard M27-A2 (NCCLS, 2002). All the experiments were carried out in triplicate. Results showed the Minimum Inhibitory Concentration (MIC) determination by microdilution method in broth showed Ocimum basilicum L. and Coriandrum sativum L. extract oils presented inhibitory activity before S. mutans strain. Basil in 1:4 concentration is bacteriostatic and in 1:3 concentration is bactericide. Coriander in 1:2 concentration is bacteriostatic and in 1:1 concentration is bactericide. We concluded that basil presented higher inhibitory activity regarding to the coriander. We also observed as bigger the extract dilution, lower their effectivity.o assess the attitude and practice of dental professionals towards using of advance Radiographic technique.

Douglas-fir root-associated microorganisms with inhibitory activity towards fungal plant pathogens and human bacterial pathogens

1996 ◽  
Vol 42 (7) ◽  
pp. 690-700 ◽  
Author(s):  
Paige E. Axelrood ◽  
Alison M. Clarke ◽  
Reed Radley ◽  
S. Janet V. Zemcov
Keyword(s):  
Antifungal Activity ◽  
Inhibitory Activity ◽  
Plant Pathogens ◽  
Bacterial Pathogens ◽  
Douglas Fir ◽  
Microbial Culture ◽  
Bacterial Strains ◽  
Fungal Plant Pathogens ◽  
Forest Sites

A microbial culture collection composed of 1820 bacterial strains, including 298 actinomycete strains, was established from the roots of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seedlings harvested from conifer nurseries and forest sites. Two hundred and thirty-four strains inhibited the growth of Fusarium, Cylindrocarpon, and (or) Pythium spp. in in vitro assays. A significantly greater proportion of bacterial strains from actinomycete genera exhibited antifungal properties compared with bacterial strains from nonactinomycete genera. Eighty-nine percent of identified inhibitory strains were Streptomyces, Streptoverticillium, Bacillus, Pseudomonas, or Burkholderia species. The actinomycete species were isolated almost exclusively from forest seedlings. Recovery of inhibitory strains representing 29 microbial species was enhanced using a variety of methods to isolate microorganisms from the roots of seedlings from nursery and forest sites. Bacterial strains (including actinomycete strains) with antifungal activity were tested for in vitro growth inhibition of six clinical human bacterial pathogens (Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa). Forty-eight percent of the tested strains inhibited one or more human pathogens. Inhibitory activity towards fungal and bacterial pathogens was strain specific, not species specific, and many inhibitory strains exhibited broad-spectrum activity. Strains with antifungal activity against several conifer root pathogens were also more likely to inhibit multiple species of clinical bacterial pathogens.Key words: in vitro, antimicrobial, conifer rhizosphere.

Screening of selected plant extracts for in vitro inhibitory activity on Human Immunodeficiency Virus

2002 ◽  
Vol 16 (6) ◽  
pp. 550-554 ◽  
Author(s):  
L. M. Bedoya ◽  
S. Sanchez Palomino ◽  
M. J. Abad ◽  
P. Bermejo ◽  
J. Alcami
Keyword(s):  
Human Immunodeficiency Virus ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Immunodeficiency Virus

scholarly journals The Effectiveness of Antibiotics Against Some Bacterial Plant Pathogens

1953 ◽  
Vol 6 (3) ◽  
pp. 481 ◽  
Author(s):  
JHE Mackay ◽  
Janet N Friend
Keyword(s):  
Plant Pathogens ◽  
Bacterial Pathogens ◽  
In Vitro Tests ◽  
Brassica Spp ◽  
Dwarf Bean

In vitro tests of six purified antibiotics against bacterial pathogens of dwarf bean, tomato, and Brassica spp. showed that streptomycin and aureomycin were slightly more effective than terramycin, and all three were more effective than penicillin, bacitracin, and chloromycetin

scholarly journals In Vitro Antituberculosis Activities of ACH-702, a Novel Isothiazoloquinolone, against Quinolone-Susceptible and Quinolone-Resistant Isolates

2010 ◽  
Vol 54 (8) ◽  
pp. 3478-3480 ◽  
Author(s):  
Michael J. Pucci ◽  
Maria Ackerman ◽  
Jane A. Thanassi ◽  
Carolyn M. Shoen ◽  
Michael H. Cynamon
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Mycobacterium Tuberculosis ◽  
Inhibitory Activity ◽  
Bacterial Pathogens ◽  
Biochemical Assays

ABSTRACT ACH-702 is a new isothiazoloquinolone with potent in vitro and in vivo activities against important bacterial pathogens, including Staphylococcus aureus. In this study, ACH-702 was found to have promising in vitro antibacterial activity against Mycobacterium tuberculosis, with MICs of ≤1 μg/ml, comparable to that of the fluoroquinolone moxifloxacin for quinolone-susceptible isolates but superior to that for quinolone-resistant isolates. Biochemical assays involving M. tuberculosis gyrase enzymes indicated that ACH-702 had significantly improved inhibitory activity compared with fluoroquinolones.

scholarly journals IN VITRO ALPHA-GLUCOSIDASE INHIBITORY ACTIVITY OF EGYPTIAN PLANT EXTRACTS AS AN INDICATION FOR THEIR ANTIDIABETIC ACTIVITY

2018 ◽  
Vol 11 (7) ◽  
pp. 360 ◽  
Author(s):  
May A El-manawaty ◽  
Lamiaa Gohar
Keyword(s):  
Plant Extracts ◽  
Inhibitory Activity ◽  
The Body ◽  
Antidiabetic Activity ◽  
Pinus Roxburghii ◽  
Systematic Screening ◽  
Ic50 Values ◽  
Rich Flora ◽  
Alpha Glucosidase

Objective: Diabetes mellitus is a highly prevalent chronic disease in Egypt leading to high socioeconomic problems, especially in the cities due to the unhealthy life style. Although many drugs are available, they have many side effects. Furthermore, the body arouses resistance after a while for the drug so it should be changed every once in a while. Plants could be a good source for drugs. In Egypt, we have a rich flora which has not been subjected to systematic screening for antidiabetic activity.Methods: The aim of this work was to screen 264 plant extracts for their in vitro α-glucosidase inhibitory activity. Those extracts which gave more than 70% inhibition were screened on different concentrations and their inhibitory concentrations giving 50% activity (IC50) were calculated.Results: Out of all the tested extracts, 63 gave more than or equal 70% inhibition on α-glucosidase at the tested concentration (25 ppm). After the calculation of the IC50 values, 10 extracts were chosen for further study having 5 ppm and less IC50.Conclusion: The most active plant extract is Pinus roxburghii Sarg. branches (IC50 is 2.47 ppm).

Sign in / Sign up

Export Citation Format

Share Document