Morphology and histology of the alimentary canal, salivary glands and Malpighian tubules in Pyrrhocoris apterus (Linnaeus, 1758) (Hemiptera: Pyrrhocoridae): a scanning electron and light microscopies study

2021 ◽  
Author(s):  
Nurcan Özyurt Koçakoğlu
Keyword(s):  
Salivary Glands ◽  
Alimentary Canal ◽  
Malpighian Tubules ◽  
Pyrrhocoris Apterus ◽  
Scanning Electron

scholarly journals Dynamics of Tomato spotted wilt virus Replication in the Alimentary Canal of Two Thrips Species

2002 ◽  
Vol 92 (7) ◽  
pp. 729-733 ◽  
Author(s):  
F. M. de Assis Filho ◽  
R. A. Naidu ◽  
C. M. Deom ◽  
J. L. Sherwood
Keyword(s):  
Salivary Glands ◽  
Virus Replication ◽  
Time Course ◽  
Alimentary Canal ◽  
Tomato Spotted Wilt Virus ◽  
Nonstructural Protein ◽  
Malpighian Tubules ◽  
Tomato Spotted Wilt ◽  
Thrips Species ◽  
Wilt Virus

Transmission of Tomato spotted wilt virus (TSWV) is dependent on virus uptake in the midgut prior to virus movement to the salivary glands. Replication of TSWV in the alimentary canal of tobacco thrips (TT, Frankliniella fusca) and western flower thrips (WFT, F. occidentalis) was investigated by immunolocalization of the nonstructural protein (NSs) encoded by the small RNA of TSWV and fluorescence microscopy. Analysis of cohorts during development from larva to adults following virus acquisition by first instar larva indicated that virus replication followed a specific time-course pattern in the foregut, regions of the midgut, salivary glands, and ligaments between the midgut and salivary glands. Initial virus replication occurred only in epithelial cells of midgut-1 but, upon infection of muscle cells, the virus moved to the midgut-2, foregut, midgut-3, and salivary glands. The ligaments between the midgut and salivary glands appeared to be a route for virus to invade the salivary glands. No virus replication was observed in the hindgut, Malpighian tubules, or tubular salivary glands. The dynamics of TSWV replication, as measured by NSs accumulation, were similar in both TT and WFT.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

scholarly journals Progression of Watermelon Bud Necrosis Virus Infection in Its Vector, Thrips palmi

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 392
Author(s):  
Amalendu Ghosh ◽  
Priti ◽  
Bikash Mandal ◽  
Ralf G. Dietzgen
Keyword(s):  
Virus Infection ◽  
Salivary Glands ◽  
Frankliniella Occidentalis ◽  
Western Flower Thrips ◽  
Malpighian Tubules ◽  
Necrosis Virus ◽  
Posterior Portion ◽  
Thrips Palmi ◽  
Thrips Species ◽  
Anterior Midgut

Thrips are important pests of agricultural, horticultural, and forest crops worldwide. In addition to direct damages caused by feeding, several thrips species can transmit diverse tospoviruses. The present understanding of thrips–tospovirus relationships is largely based on studies of tomato spotted wilt virus (TSWV) and Western flower thrips (Frankliniella occidentalis). Little is known about other predominant tospoviruses and their thrips vectors. In this study, we report the progression of watermelon bud necrosis virus (WBNV) infection in its vector, melon thrips (Thrips palmi). Virus infection was visualized in different life stages of thrips using WBNV-nucleocapsid protein antibodies detected with FITC-conjugated secondary antibodies. The anterior midgut was the first to be infected with WBNV in the first instar larvae. The midgut of T. palmi was connected to the principal salivary glands (PSG) via ligaments and the tubular salivary glands (TSG). The infection progressed to the PSG primarily through the connecting ligaments during early larval instars. The TSG may also have an ancillary role in disseminating WBNV from the midgut to PSG in older instars of T. palmi. Infection of WBNV was also spread to the Malpighian tubules, hindgut, and posterior portion of the foregut during the adult stage. Maximum virus-specific fluorescence in the anterior midgut and PSG indicated the primary sites for WBNV replication. These findings will help to better understand the thrips–tospovirus molecular relationships and identify novel potential targets for their management. To our knowledge, this is the first report of the WBNV dissemination path in its vector, T. palmi.

scholarly journals The Genes raw and ribbon Are Required for Proper Shape of Tubular Epithelial Tissues in Drosophila

Genetics ◽  
1997 ◽  
Vol 147 (1) ◽  
pp. 243-253 ◽  
Author(s):  
Joseph Jack ◽  
Guy Myette
Keyword(s):  
Embryonic Development ◽  
Salivary Glands ◽  
Cell Shape ◽  
Malpighian Tubules ◽  
Dorsal Closure ◽  
Epithelial Tissues ◽  
Cell Rearrangement

Abstract The products of two genes, raw and ribbon (rib), are required for the proper morphogenesis of a variety of tissues. Malpighian tubules mutant for raw or rib are wider and shorter than normal tubules, which are only two cells in circumference when they are fully formed. The mutations alter the shape of the tubules beginning early in their formation and block cell rearrangement late in development, which normally lengthens and narrows the tubes. Mutations of both genes affect a number of other tissues as well. Both genes are required for dorsal closure and retraction of the CNS during embryonic development. In addition, rib mutations block head involution, and broaden and shorten other tubular epithelia (salivary glands, tracheae, and hindgut) in much same manner as they alter the shape of the Malpighian tubules. In tissues in which the shape of cells can be observed readily, rib mutations alter cell shape, which probably causes the change in shape of the organs that are affected. In double mutants raw enhances the phenotypes of all the tissues that are affected by rib but unaffected by raw alone, indicating that raw is also active in these tissues.

scholarly journals Digestive And Tracheal System of Menopon Gallinae (Phthiraptera: Amblycera) Infesting Poultry Bird (Gallus Gallus Domesticus)

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Surman Arya ◽  
Suneel Kumar Singh
Keyword(s):  
Alimentary Canal ◽  
Gallus Domesticus ◽  
Malpighian Tubules ◽  
Gallus Gallus Domesticus ◽  
Tracheal System ◽  
Visceral Organs ◽  
Binocular Microscope ◽  
Hind Gut ◽  
Poultry Shaft Louse ◽  
Alary Muscles

The digestive and tracheal system of a poultry shaft louse, Menopon gallinae (Phthiraptera: Amblycera) has been studied in greater details. Alimentary canal of louse was dissected out along with crop under stereozoom binocular microscope. Entire alimentary canal of M. gallinae was found more or less straight and has three basic parts (fore-gut, mid-gut and hind-gut) while crop-teeth was present in the crop. The posterior end of crop contains 20-30 well developed crop-teeth arranged in a single arced plate, in comb-like fashion. Mid-gut was found as simple tube and contributes nearly one half of the total length, while the hind-gut was marked by the opening of Malpighian tubules in alimentary canal. The heart of M. gallinae is of simplest kind, one chambered bulbous structure having three pairs of laterally placed ostia and supported by four pairs of alary muscles. In the tracheal system there were seven pairs of spiracles occurred on the terga of M. gallinae. The first pair of spiracle was found located close to legs while remaining six abdominal spiracles occur from segment 3rd to 8th. The degree of tracheation of various visceral organs has also been noted.

scholarly journals Obligate development of Blastocrithidia papi (Trypanosomatidae) in the Malpighian tubules of Pyrrhocoris apterus (Hemiptera) and coordination of host-parasite life cycles

PLoS ONE ◽  
2018 ◽  
Vol 13 (9) ◽  
pp. e0204467 ◽  
Author(s):  
Alexander O. Frolov ◽  
Marina N. Malysheva ◽  
Anna I. Ganyukova ◽  
Vyacheslav Yurchenko ◽  
Alexei Y. Kostygov
Keyword(s):  
Life Cycles ◽  
Malpighian Tubules ◽  
Pyrrhocoris Apterus ◽  
Host Parasite

On the morphology of developing eggs of the camel tick Hyalomma dromedarii Koch, 1844

1986 ◽  
Vol 64 (9) ◽  
pp. 1994-1997 ◽  
Author(s):  
M. El Gohary ◽  
M. Y. Kamel ◽  
M. H. Madbouly
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Trypan Blue ◽  
Egg Laying ◽  
Malpighian Tubules ◽  
Hyalomma Dromedarii ◽  
Outermost Layer ◽  
Camel Tick ◽  
Egg Morphology ◽  
Scanning Electron

External egg morphology of the camel tick Hyalomma dromedarii was studied. Injection of egg-laying females with 1% trypan blue resulted in incorporation of vital stain in the oviposited eggs. Daily measurements of fresh eggs revealed changes in egg length and width during embryonic development. Scanning electron microscopy showed the outermost layer of the egg to be spongy and porous containing clear pits of different sizes. An elevated longitudinal hatching ridge encircled the egg chorion. The development of cephalothorax, appendages, Malpighian tubules, and rectal sacs were monitered.

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