Oleanolic acid content in Baccharis linearis and its effects on Heliothis zea larvae

<p>Research on the growth and oleanolic acid content of pearl grass (Hedyotis corymbosa) in experimentall field of Medicinal Plants and Traditional Medicines Research and Development Center (B2P2TOOT), Tanjungsari Village, Tegal Gede Village, Karanganyar and B2P2TOOT laboratory, Tawangmangu, Karanganyar. The research has been conducted in August to November 2017. The purpose of this research is to know the dosage of cow manure and liquid organic manure which has the most influence to the growth and the oleanolic acid content of pearl grass. The experiment was designed in a randomized complete block design with twelve treatments and three repication. The observed data analyzed using variance analysis with 5% level then if there was a significant difference in the continued test with Duncan’s Multiple Range Test (DMRT). The results showed that the use of cow manure at doses of 0 kg-1m2, 4,6 kg-1m2, 6,1 kg-1m2 and 7,6 kg-1m2 did not give increase to all observation variables. The use of POC dose 0 ml-1liter, 5 ml-1liter and 10 ml-1liter did not give increase to all observation variables. All given treatments may show that oleanolic acid is present in pearl grasses.</p>

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Growth and oleanolic acid accumulation of Polyscias fruticosa cell suspension cultures

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666201119123503 ◽

2020 ◽

Vol 21 ◽

Author(s):

Phan Thi A Kim ◽

Le Thi Binh An ◽

Nguyen Thanh Chung ◽

Nguyen Tien Truong ◽

Le Thi Anh Thu ◽

...

Keyword(s):

Cell Culture ◽

Cell Growth ◽

Carbon Source ◽

Cell Suspension ◽

Oleanolic Acid ◽

Acid Content ◽

Dry Weight ◽

Stem Segment ◽

Anticancer Activities ◽

Study Results

Background: Oleanolic acid is an oleanane triterpene found in many plant species all over the world. This compound is also a major saponin in leaves of Polyscias fruticosa and possess several promising pharmacological activities, such as hepatoprotective effects, and anti-inflammatory, antioxidant, or anticancer activities. Objective: The objective of the present work is to establish cell suspension culture of P. fruticosa, investigate influence of several factors such as plant growth regulators and carbon source on cell growth, and determine their oleanolic acid content. Method: Cell culture was carried out by using 2 g fresh weight of 30 day old friable callus derived from in vitro stem segment in 50 mL of liquid medium with a shaking speed of 120 rpm. Culture was then incubated at 25±2ºC with a shaking speed of 120 rpm in the period of 12 h daylight at light intensity of about 6.75 µmol/m2 /s. Cell growth was measured by fresh and dry biomass at 16h day. Oleanolic acid content was determined using HPLC analysis. Results & Discussion: The study results showed that MS medium containing 2% sucrose as a carbon source, supplemented with 1 mg/L 6-benzylaminopurine and 0.5 mg/L 2,4-D dichlorophenoxyacetic acid was the most appropriate growth medium. Cell biomass and oleanolic acid content reached the highest values of 0.43 g dry weight/flask and 25.4 mg/g dry weight, respectively. Conclusion: These results indicated the potential production of oleanolic acid, a compound with high pharmacological value, from P. fruticosa cell culture.

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Effect of light intensity on the ultrastructure of the filamentous cyanobacterium, Anabaena variabilis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100103565 ◽

1988 ◽

Vol 46 ◽

pp. 300-301

Author(s):

C. S. Bricker ◽

S. R. Barnum ◽

B. Huang ◽

J. G. Jaworskl

Keyword(s):

Fatty Acid ◽

Light Intensity ◽

Acid Content ◽

Fatty Acid Content ◽

Anabaena Variabilis ◽

Chloroplast Envelope ◽

Major Constituent ◽

Filamentous Cyanobacterium ◽

Photosynthetic Membrane ◽

Effect Of Light

Cyanobacteria are Gram negative prokaryotes that are capable of oxygenic photosynthesis. Although there are many similarities between eukaryotes and cyanobacteria in electron transfer and phosphorylation during photosynthesis, there are two features of the photosynthetic apparatus in cyanobacteria which distinguishes them from plants. Cyanobacteria contain phycobiliproteins organized in phycobilisomes on the surface of photosynthetic membrane. Another difference is in the organization of the photosynthetic membranes. Instead of stacked thylakolds within a chloroplast envelope membrane, as seen In eukaryotes, IntracytopIasmlc membranes generally are arranged in three to six concentric layers. Environmental factors such as temperature, nutrition and light fluency can significantly affect the physiology and morphology of cells. The effect of light Intensity shifts on the ultrastructure of Internal membrane in Anabaena variabilis grown under controlled environmental conditions was examined. Since a major constituent of cyanobacterial thylakolds are lipids, the fatty acid content also was measured and correlated with uItrastructural changes. The regulation of fatty acid synthesis in cyanobacteria ultimately can be studied if the fatty acid content can be manipulated.

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