Reversal of cholinesterase inhibition in plasma, red blood cells and diaphragm; Clinical signs and post-mortem findings in mice after intraperitoneal injection of anatoxin-A(s), paraoxon or pyridostigmine

Toxicon ◽  
1989 ◽  
Vol 27 (1) ◽  
pp. 38-39
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Intraperitoneal Injection ◽  
Clinical Signs ◽  
Cholinesterase Inhibition ◽  
Post Mortem ◽  
Post Mortem Findings

scholarly journals Improvement of bovine tuberculosis diagnosis

2021 ◽  
pp. 261-265
Author(s):  
M. O. Baratov
Keyword(s):  
Red Blood Cells ◽  
Mycobacterium Bovis ◽  
Blood Cells ◽  
Bovine Tuberculosis ◽  
Intradermal Test ◽  
Mycobacterium Fortuitum ◽  
Post Mortem ◽  
Post Mortem Examination ◽  
Antibody Titers ◽  
Specific Reactions

Detection of animals with non-specific reactions to tuberculin is one of the major problems in bovine tuberculosis (TB) diagnosis. There is a need to find and improve methods for detection of the sensitization causes. This paper presents the results of comparative studies of different ways to stabilize red blood cells in order to obtain diagnosticums for indirect hemagglutination (IHA) test. The article describes the stages of red blood cells stabilization and sensitization and demonstrates the diagnostic significance of Fili stabilization method using formaldehyde as a fixative. The highest antibody titers (1:3000 and 1:4000) were received in hyperimmune sera of rabbits immunized with Mycobacterium bovis using a homologous diagnosticum. Practical importance of the sensitins homologous to the infection is shown during testing of 1,911 serum samples collected from animals of different categories (diseased; healthy and reacting to tuberculin; healthy and not reacting to tuberculin) with IHA test using diagnosticums produced from Mycobacterium bovis and Mycobacterium fortuitum. Based on the positive results of the IHA test, TB was diagnosed in 87.5% of animals originating from an infected farm during post-mortem examination. The results of the IHA test agreed with those of the intradermal tuberculin test in 37.7% of cases. Diagnostic antibody titers were found in 206 TB infected animals with no reaction to the intradermal test. However, the post-mortem examination revealed TB changes in internal organs. The obtained data suggest a possibility to use the IHA test to detect TB infected animals with non-specific reactions to tuberculin.

The development and pathogenesis of Leucocytozoon simondi in Canada and domestic geese in Algonquin Park, Ontario

1976 ◽  
Vol 54 (5) ◽  
pp. 634-643 ◽  
Author(s):  
Sherwin S. Desser ◽  
Andrée K. Ryckman
Keyword(s):  
Red Blood Cells ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Primary Infection ◽  
Clinical Signs ◽  
Peripheral Circulation ◽  
Branta Canadensis ◽  
Canada Geese ◽  
Severe Anemia ◽  
Branta Canadensis Interior

The development of Leucocytozoon simondi was studied in naturally and experimentally infected Branta canadensis maxima, Branta canadensis interior, and Anser domesticus. The number of mature round gametocytes in the peripheral blood of the Canada geese increased between days 9 and 15 post exposure (PE) and decreased rapidly thereafter. Mean peak parasitemias recorded on day 13 PE were (per 1000 red blood cells (RBC)): 8 gametocytes in B.c. maxima, 16 gametocytes in B.c. interior, and 17 gametocytes in A. domesticus. About 3 weeks PE, gametocytes disappeared from the peripheral circulation and were not observed again during the autumn, winter, and spring in birds kept in the laboratory.Haematocrit determinations in the Canada geese revealed a low fluctuating anemia during the primary infection which subsided by day 21 PE. A more severe anemia was recorded in A. domesticus with a mean low packed RBC value of about 18% on day 11 PE. Immature and mature hepatic schizonts were observed in the Canada and domestic geese between days 3 and 8 PE. Neither megaloschizonts nor elongate gametocytes were seen. Clinical signs, pathology, and mortality commonly associated with L. simondi infection in ducks were not observed. Hypotheses are advanced to explain reports of severe pathogenesis associated with L. simondi infections in Canada geese in other localities.

Establishment of a murine model of cerebral malaria in KunMing mice infected with Plasmodium berghei ANKA

Parasitology ◽  
2016 ◽  
Vol 143 (12) ◽  
pp. 1672-1680 ◽  
Author(s):  
YAN DING ◽  
WENYUE XU ◽  
TAOLI ZHOU ◽  
TAIPING LIU ◽  
HONG ZHENG ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Cerebral Malaria ◽  
Plasmodium Berghei ◽  
Blood Cells ◽  
Mononuclear Cells ◽  
Clinical Signs ◽  
Necrosis Factor Alpha ◽  
Experimental Cerebral Malaria ◽  
Km Mice

SUMMARYMalaria remains one of the most devastating diseases. Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection resulting in high mortality and morbidity worldwide. Analysis of precise mechanisms of CM in humans is difficult for ethical reasons and animal models of CM have been employed to study malaria pathogenesis. Here, we describe a new experimental cerebral malaria (ECM) model with Plasmodium berghei ANKA infection in KunMing (KM) mice. KM mice developed ECM after blood-stage or sporozoites infection, and the development of ECM in KM mice has a dose-dependent relationship with sporozoites inoculums. Histopathological findings revealed important features associated with ECM, including accumulation of mononuclear cells and red blood cells in brain microvascular, and brain parenchymal haemorrhages. Blood–brain barrier (BBB) examination showed that BBB disruption was present in infected KM mice when displaying clinical signs of CM. In vivo bioluminescent imaging experiment indicated that parasitized red blood cells accumulated in most vital organs including heart, lung, spleen, kidney, liver and brain. The levels of inflammatory cytokines interferon-gamma, tumour necrosis factor-alpha, interleukin (IL)-17, IL-12, IL-6 and IL-10 were all remarkably increased in KM mice infected with P. berghei ANKA. This study indicates that P. berghei ANKA infection in KM mice can be used as ECM model to extend further research on genetic, pharmacological and vaccine studies of CM.

scholarly journals Enterocolitis Caused by Ehrlichia sp. in the Horse (Potomac Horse Fever)

1986 ◽  
Vol 23 (4) ◽  
pp. 471-477 ◽  
Author(s):  
D. O. Cordes ◽  
B. D. Perry ◽  
Y. Rikihisa ◽  
W. R. Chickering
Keyword(s):  
Mast Cells ◽  
Epithelial Cells ◽  
Acute Phase ◽  
Whole Blood ◽  
Clinical Signs ◽  
Post Mortem ◽  
Potomac Horse Fever ◽  
Incubation Periods ◽  
Mucous Membranes ◽  
Post Mortem Findings

Potomac horse fever was reproduced in 15 ponies by transfusion of whole blood originally from two natural cases and subsequently from ponies infected by the transfusions. Incubation periods varied from 9 to 15 days. Affected ponies developed varying degrees of fever, diarrhea, anorexia, depression, and leukopenia. Eleven affected ponies were killed, three died in the acute phase of the disease, and one did not show clinical signs. The most consistent post-mortem findings were fluid contents in the cecum and large colon, and areas of hyperemia (of inconstant degree and distribution) in mucosae of both small and large intestines. Multifocal areas of necrosis occurred in mucous membranes. Ehrlichial organisms were most common in the cytoplasm of epithelial cells, macrophages, and mast cells of the large colon.

Studies on Tyzzer's disease in rats

1978 ◽  
Vol 12 (1) ◽  
pp. 1-4 ◽  
Author(s):  
A. Schaich Fries ◽  
O. Svendsen
Keyword(s):  
Antibody Titre ◽  
Causative Agent ◽  
Clinical Signs ◽  
Post Mortem ◽  
Immunofluorescence Technique ◽  
Epidemic Disease ◽  
Breeding Colony ◽  
Indirect Immunofluorescence Technique ◽  
Post Mortem Findings ◽  
The Individual

An outbreak of an epidemic disease occurred in a specified-pathogen-free (SPF) breeding colony of rats. The clinical signs and the post-mortem findings were characteristic for Tyzzer's disease. The causative agent, Bacillus piliformis, was demonstrated microscopically in ileum, liver and myocardium, and transmitted to mice where its pathogenicity appeared to be similar to that of another strain isolated from mice. B. piliformis from spontaneously-infected rats was demonstrated by indirect immunofluorescence technique. By means of the same technique it was found that the fluorescence antibody titre obtained of the individual sera from spontaneously-infected mice, rats and rabbits was the same, whether the antigen employed was organisms isolated from rats or mice. By testing sera from healthy rats in 3 different colonies by use of immunofluorescence technique, antibodies were found in several sera.

scholarly journals Enterotoxaemia involving Clostridium perfringens iota toxin in a hysterectomy-derived rabbit colony

1980 ◽  
Vol 14 (4) ◽  
pp. 347-351 ◽  
Author(s):  
P. Eaton ◽  
D. S. Fernie
Keyword(s):  
Clostridium Perfringens ◽  
Clinical Signs ◽  
Post Mortem ◽  
Toxin A ◽  
Clostridium Perfringens Iota Toxin ◽  
Post Mortem Findings

During an explosive outbreak of fatal enteropathic disease involving Clostridium perfringens iota (i) toxin. a total of 183 deaths occurred in 18 weeks. The clinical signs and post-mortem findings are reported. Examinations for virus, Bacillus piliformis and coccidia were negative. Clostridium perfringens i toxin was detected in 22 of 27 animals examined (81·5%), but clostridia were not isolated. Various treatments wcre attempted. It is concluded that i toxin and the syndrome described are closely related.

scholarly journals The danger of the spread of Dirofilaria immitis in dogs in Central Yakutia, medical and preventive and sanitary measures

2021 ◽  
Author(s):  
L.Yu. Gavrilyeva ◽  
L.M. Kokolova ◽  
E.V. Sivtseva ◽  
S.M. Stepanova ◽  
S.V. Dulova ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Fatal Outcome ◽  
Clinical Signs ◽  
Diagnostic Methods ◽  
Destructive Effect ◽  
Central Yakutia ◽  
Blood Smears ◽  
Difficulty Breathing ◽  
Liver And Kidney

The parasitic disease Dirofilariasis has been detected in Central Yakutia for the last 3 years and deserves the closest attention from researchers and practicing veterinarians. In dogs infected with the nematode of the genus Dirofilaria and infected with in rural (suburban) dogs, the main pathomorphological changes were localized in the heart and were represented by atrophic, dystrophic and necrobiotic processes. In addition, the products of nematode metabolism led to the appearance of circulatory disorders. First of all, the researchers paid attention to the study of blood smears, a study of the microfilariae found in the smears. The authors also examined guard dogs that died from this disease, and not a complete examination of organs and tissues during the autopsy of the heart, luvers and parenchymatosis organs, sexually mature dirofilariae were found in the heart of the dissected fallen dogs. Subsequently, diagnostic methods were used to study peripheral blood, and live Microfilariae larvae were found. As the authors of the article note, cardiac Dirofilariasis subsequently had a destructive effect on red blood cells - red blood cells, and was observed in the development of Hemoglobinemia, Hemoglobinuria, in some cases leading to liver and kidney failure. On the part of the respiratory system, there were also characteristic clinical signs of chronic dry cough, difficulty breathing and shortness of breath, the detection of foci of wheezing in the lungs. During the disease, the animals had the development of pulmonary Thromboembolism characterized by the occurrence of fever and the release of sputum with blood. In dirofilariasis, the authors observed a fatal outcome, the corpses of the fallen animals were opened for examination of organs and tissues to detect nematodes.

Sign in / Sign up

Export Citation Format

Share Document