Stimulation of fetal thymidine kinase in cultured human fibroblasts transformed by SV40 virus

Virology ◽  
1974 ◽  
Vol 57 (1) ◽  
pp. 279-284 ◽  
Author(s):  
David L. Bull ◽  
Andrew T. Taylor ◽  
Donna M. Austin ◽  
Oliver W. Jones
Keyword(s):  
Thymidine Kinase ◽  
Human Fibroblasts ◽  
Sv40 Virus ◽  
Cultured Human Fibroblasts ◽  
Stimulation Of

Cytosolic thymidine kinase activity in cultured human fibroblasts from individuals with galactokinase deficiency

1989 ◽  
Vol 27 (3-4) ◽  
pp. 219-228 ◽  
Author(s):  
J. Baptista ◽  
M. Brivet ◽  
N. Kadhom ◽  
M. Gautier ◽  
A. Lemonnier
Keyword(s):  
Thymidine Kinase ◽  
Kinase Activity ◽  
Human Fibroblasts ◽  
Thymidine Kinase Activity ◽  
Cultured Human Fibroblasts

Insulin stimulation of glucose entry in cultured human fibroblasts

1979 ◽  
Vol 101 (1) ◽  
pp. 129-138 ◽  
Author(s):  
Barbara V. Howard ◽  
David M. Mott ◽  
Rose M. Fields ◽  
Peter H. Bennett
Keyword(s):  
Human Fibroblasts ◽  
Insulin Stimulation ◽  
Cultured Human Fibroblasts ◽  
Stimulation Of

Influx and efflux of 3-O-methyl-D-glucose by cultured human fibroblasts

1988 ◽  
Vol 254 (5) ◽  
pp. C628-C633 ◽  
Author(s):  
N. Longo ◽  
L. D. Griffin ◽  
L. J. Elsas
Keyword(s):  
Transport Process ◽  
Glucose Transporter ◽  
Cytochalasin B ◽  
Human Fibroblasts ◽  
Functional Asymmetry ◽  
Maximal Velocity ◽  
Cultured Human Fibroblasts ◽  
Kinetics Of ◽  
Stimulation Of

We measured the initial rates of 3-O-methyl-D-glucose (OMG) fluxes by cultured human fibroblasts. D-Glucose (300 mM) and cytochalasin B (5 microM) inhibited approximately 80% of OMG (1 mM) influx. OMG rapidly entered human fibroblasts, and influx was linear up to 20 s. OMG influx and efflux were about equal. Cytochalasin B inhibited OMG (1 mM) influx and efflux within 20 s of exposure. Cytochalasin B half maximally inhibited OMG influx and efflux at 0.51 and 0.75 microM, respectively. In zero trans conditions, the kinetics of OMG influx and efflux were similar. However, when OMG was present on the trans side of the membrane, OMG influx but not OMG efflux was stimulated. Trans stimulation of OMG influx increased the maximal velocity of this transport process, without affecting its Km. These results suggest that 1) OMG influx and efflux occur through the same transporter, and 2) the glucose transporter of cultured human fibroblasts presents a functional asymmetry when substrate is present on the trans side of the membrane.

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