Effect of squid extracts on time course appearance of glucose and free amino acids in haemolymph in Penaeus japonicus after feeding: Preliminary results

The time course of entry of radioactive carbon from intravenously administered [U-14C]-glucose into protein in five brain regions in rats was studied using an automatic amino acid analyzer coupled, through a flow cell, with a scintillation counter. Radioactivity appeared in protein-bound alanine and in glutamic and aspartic acids and, more slowly and to a much lesser extent, in serine, proline, and glycine; none was detected in any other bound amino acids. There appear to be two main groups of proteins, one of which turns over more slowly than the other. In pentobarbital narcosis, radioactivity in protein-bound alanine increased but no other changes were obvious. In convulsions induced by pentylenetetrazol or picrotoxin, and during hypoxia, the entry of radioactive carbon into protein-bound alanine, glutamic acid, and aspartic acid decreased. At 24 h after the injection of radioactive glucose, radioactivity in free amino acids had almost completely disappeared in normal animals though protein remained radioactive. Pentobarbital narcosis did not affect this situation. Radioactivity was released from protein into free amino acids during hypoxia, during convulsions induced by pentylenetetrazol, picrotoxin, or oxygen at high pressure, and after insulin treatment. Evidence was obtained that free alanine occurs in more than one pool.

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The contents of free amino acids and elements in As-hyperaccumulator Pteris cretica and non-hyperaccumulator Pteris straminea during reversible senescence

Plant Soil and Environment ◽

10.17221/606/2017-pse ◽

2017 ◽

Vol 63 (No. 10) ◽

pp. 455-460 ◽

Cited By ~ 1

Author(s):

Pavlíková Daniela ◽

Zemanová Veronika ◽

Pavlík Milan

Keyword(s):

Amino Acids ◽

Principal Component Analysis ◽

Free Amino Acids ◽

Free Amino ◽

Time Course ◽

Principal Component ◽

Pteris Cretica ◽

Stress Metabolites ◽

The Relationship ◽

Hyperaccumulator Plant

The objectives of this study were to analyse the relationship between the contents of elements and free amino acids (AAs) in fronds of As-hyperaccumulator Pteris cretica cv. Albo-lineata (PC) and non-hyperaccumulator Pteris straminea (PS) during reversible senescence. The time-course effect on senescence was also investigated. The two ferns were grown in a pot experiment with soil containing 16 mg As<sub>total</sub>/kg soil for 160 days. The contents of elements and AAs in both ferns and in individual sampling periods differed. The highest accumulation of elements and AAs was measured in PS fronds after 83 days; however, the accumulation of As, Ca, Cu, Fe, Mg, P and asparagin in PC fronds was highest after 160 days. The results of principal component analysis showed more rapid senescence of PS compared to PC. This was caused by changes in the relationship between the contents of elements (cofactors of metalloenzymes, stress metabolites) and AAs (transport of NH<sub>2</sub> group and stress metabolites). The hyperaccumulator plant (PC) was more resistant than the bioindicator plant (PS) to the conversion from reversible to irreversible senescence.

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A Note on the Free Amino Acids in Some Small Species of Pogonophora

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s002531540002422x ◽

1980 ◽

Vol 60 (1) ◽

pp. 171-174 ◽

Cited By ~ 5

Author(s):

A. J. Southward ◽

D. R. Dixon

Keyword(s):

Amino Acids ◽

Organic Compounds ◽

Free Amino Acids ◽

Free Amino ◽

Whole Body ◽

Small Species ◽

Preliminary Results ◽

Lack Of Information ◽

Dissolved Organic Compounds

Previous studies on the significance of dissolved organic compounds in the nutrition of the smaller species of the phylum Pogonophora (Little & Gupta, 1968, 1969; Southward & Southward, 1968, 1970) were handicapped by lack of information about the levels of free amino acids in the tissues. In this brief note we give preliminary results for four species, using a modification of the technique described by Clark (1964, 1968, 1973) to estimate the amino acids in extracts of the whole body.

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