Time course of the content of folates and free amino acids in leaves of Pisum sativum L. after irradiation with ultraviolet C

2005 ◽  
Vol 32 (2) ◽  
pp. 121-127 ◽  
Author(s):  
L. F. Stakhov ◽  
V. G. Ladygin ◽  
L. N. Stakhova
1970 ◽  
Vol 48 (3) ◽  
pp. 236-243 ◽  
Author(s):  
Y. Yoshino ◽  
K. A. C. Elliott

The time course of entry of radioactive carbon from intravenously administered [U-14C]-glucose into protein in five brain regions in rats was studied using an automatic amino acid analyzer coupled, through a flow cell, with a scintillation counter. Radioactivity appeared in protein-bound alanine and in glutamic and aspartic acids and, more slowly and to a much lesser extent, in serine, proline, and glycine; none was detected in any other bound amino acids. There appear to be two main groups of proteins, one of which turns over more slowly than the other. In pentobarbital narcosis, radioactivity in protein-bound alanine increased but no other changes were obvious. In convulsions induced by pentylenetetrazol or picrotoxin, and during hypoxia, the entry of radioactive carbon into protein-bound alanine, glutamic acid, and aspartic acid decreased. At 24 h after the injection of radioactive glucose, radioactivity in free amino acids had almost completely disappeared in normal animals though protein remained radioactive. Pentobarbital narcosis did not affect this situation. Radioactivity was released from protein into free amino acids during hypoxia, during convulsions induced by pentylenetetrazol, picrotoxin, or oxygen at high pressure, and after insulin treatment. Evidence was obtained that free alanine occurs in more than one pool.


2017 ◽  
Vol 63 (No. 10) ◽  
pp. 455-460 ◽  
Author(s):  
Pavlíková Daniela ◽  
Zemanová Veronika ◽  
Pavlík Milan

The objectives of this study were to analyse the relationship between the contents of elements and free amino acids (AAs) in fronds of As-hyperaccumulator Pteris cretica cv. Albo-lineata (PC) and non-hyperaccumulator Pteris straminea (PS) during reversible senescence. The time-course effect on senescence was also investigated. The two ferns were grown in a pot experiment with soil containing 16 mg As<sub>total</sub>/kg soil for 160 days. The contents of elements and AAs in both ferns and in individual sampling periods differed. The highest accumulation of elements and AAs was measured in PS fronds after 83 days; however, the accumulation of As, Ca, Cu, Fe, Mg, P and asparagin in PC fronds was highest after 160 days. The results of principal component analysis showed more rapid senescence of PS compared to PC. This was caused by changes in the relationship between the contents of elements (cofactors of metalloenzymes, stress metabolites) and AAs (transport of NH<sub>2</sub> group and stress metabolites). The hyperaccumulator plant (PC) was more resistant than the bioindicator plant (PS) to the conversion from reversible to irreversible senescence.


1955 ◽  
Vol 65 (3) ◽  
pp. 299-316 ◽  
Author(s):  
T. G. Hyde

SynopsisThe nitrogen metabolism of both pods and seeds of Pisum sativum has been followed at sixteen stages of growth. The distribution of free amino acids and related compounds has been investigated by paper partition chromatography.


1991 ◽  
Vol 83 (1) ◽  
pp. 136-143 ◽  
Author(s):  
L. Bray ◽  
D. Chriqui ◽  
K. Gloux ◽  
D. Le Rudulier ◽  
M. Meyer ◽  
...  

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