Food restriction in female Wistar rats: V. Lipid peroxidation and antioxidant enzymes in the liver

1992 ◽  
Vol 14 (1) ◽  
pp. 93-99 ◽  
Author(s):  
C. Pieri ◽  
M. Falasca ◽  
F. Marcheselli ◽  
F. Moroni ◽  
R. Recchioni ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Food Restriction ◽  
Wistar Rats ◽  
Female Wistar Rats

scholarly journals The influence of food restriction versus ad libitum feeding of chow and purified diets on variation in body weight, growth and physiology of female Wistar rats

2012 ◽  
Vol 46 (2) ◽  
pp. 101-107 ◽  
Author(s):  
M Moraal ◽  
P P A M Leenaars ◽  
H Arnts ◽  
K Smeets ◽  
B S Savenije ◽  
...  
Keyword(s):  
Body Weight ◽  
Food Restriction ◽  
Wistar Rats ◽  
Statistical Significance ◽  
Blood Parameters ◽  
Calorie Intake ◽  
Restricted Feeding ◽  
Organ Weights ◽  
Female Wistar Rats ◽  
Ad Libitum

Ad libitum (AL) supply of standard chow is the feeding method most often used for rodents in animal experiments. However, AL feeding is known to result in a shorter lifespan and decreased health as compared with restricted feeding. Restricted feeding and thus limiting calorie intake prevents many health problems, increases lifespan and can also increase group uniformity. All this leads to a reduced number of animals needed. So-called standard chows are known to be prone to variation in composition. Synthetic foods have a more standard composition, contributing to group uniformity which, like diet reduction, may decrease the number of animals necessary to obtain statistical significance. In this study, we compared the effects of AL versus restricted feeding (25% reduction in food intake) on standard chow versus synthetic food of three different suppliers on body weight (BW), growth, several blood parameters and organ weights in growing female Wistar rats over a period of 61 days. Diet restriction led to a decreased growth and significantly reduced variation in BW and growth as compared with AL feeding. AL feeding on synthetic diets caused a significantly higher BW gain than on chow diets. Due to experimental design, this same effect occurred on food restriction. Blood parameters and organ weights were affected neither by diet type nor by amount. Incidentally, variations were significantly reduced on food restriction versus AL, and on synthetic diets versus chow diets. This study demonstrates that food restriction versus AL feeding leads to a significantly reduced variation in BW and growth, thereby indicating the potential for reduction when applying this feeding schedule.

Effect of long-term moderate-exercise combined with metformin-treatment on antioxidant enzymes activity and expression in the gastrocnemius of old female Wistar rats

2020 ◽  
Vol 21 (6) ◽  
pp. 787-805
Author(s):  
Ulalume Hernández-Arciga ◽  
David Hernández-Álvarez ◽  
Stefanie Paola López-Cervantes ◽  
Norma Edith López-Díazguerrero ◽  
Adriana Alarcón-Aguilar ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Wistar Rats ◽  
Metformin Treatment ◽  
Moderate Exercise ◽  
Enzymes Activity ◽  
Female Wistar Rats

scholarly journals Modulation of the Activities of Catalase, Cu-Zn, Mn Superoxide Dismutase, and Glutathione Peroxidase in Adipocyte from Ovariectomised Female Rats with Metabolic Syndrome

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Rebeca Cambray Guerra ◽  
Alejandra Zuñiga-Muñoz ◽  
Verónica Guarner Lans ◽  
Eulises Díaz-Díaz ◽  
Carlos Alberto Tena Betancourt ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Antioxidant Capacity ◽  
Abdominal Fat ◽  
Female Rats ◽  
Female Rat ◽  
Female Wistar Rats ◽  
And Oxidative Stress

The aim of this study was to evaluate the association between estrogen removal, antioxidant enzymes, and oxidative stress generated by obesity in a MS female rat model. Thirty two female Wistar rats were divided into 4 groups: Control (C), MS, MS ovariectomized (Ovx), and MS Ovx plus estradiol (E2). MS was induced by administering 30% sucrose to drinking water for 24 weeks. After sacrifice, intra-abdominal fat was dissected; adipocytes were isolated and lipid peroxidation, non-enzymatic antioxidant capacity, and the activities of Cu-Zn and Mn superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined. There were no significant differences in the activities of Cu-Zn, Mn SOD, CAT, and GPx between the C and MS groups, but in the MS Ovx group there was a statistically significant decrease in the activities of these enzymes when compared to MS and MS Ovx+E2. The increased lipid peroxidation and nonenzymatic antioxidant capacity found in MS Ovx was significantly decreased when compared to MS and MS Ovx+E2. In conclusion, the removal of E2by ovariectomy decreases the activity of the antioxidant enzymes in the intra-abdominal tissue of MS female rats; this is reflected by increased lipid peroxidation and decreased nonenzymatic antioxidant capacity.

Food restriction in female Wistar rats. VII. Mitochondrial parameters in resting and proliferating splenic lymphocytes

1994 ◽  
Vol 19 (1) ◽  
pp. 31-42 ◽  
Author(s):  
Carlo Pieri ◽  
Rina Recchioni ◽  
Fausto Moroni ◽  
Fiorella Marcheselli ◽  
Maurizio Marra
Keyword(s):  
Food Restriction ◽  
Wistar Rats ◽  
Splenic Lymphocytes ◽  
Female Wistar Rats

Serum leptin is not correlated with body fat in severe food restriction

2012 ◽  
Vol 37 (6) ◽  
pp. 1063-1071 ◽  
Author(s):  
Sarah E. Gairdner ◽  
Catherine E. Amara
Keyword(s):  
Body Composition ◽  
Body Mass ◽  
Body Fat ◽  
Fat Mass ◽  
Food Restriction ◽  
Wistar Rats ◽  
Serum Leptin ◽  
Wide Range ◽  
Female Wistar Rats ◽  
Ad Libitum

Leptin is an adipose-derived hormone with established roles in energy balance that can impact the response to refeeding after malnutrition. Although the amount of circulating leptin has traditionally been associated with the amount of adipose tissue, controversy exists as to whether this relationship is constant in both humans and animals and over a wide range of body composition. Our objective was to evaluate whether the leptin – body fat ratio is constant in the range of healthy to low body mass in female Wistar rats. Eight ad libitum fed (C) and eight food-restricted (FR) rats were compared over a period of four weeks. FR rats attained the target 75% of baseline body mass after the first two weeks, which was maintained for the remaining two weeks. Serum leptin and IGF-1 (ELISA) and body composition (DXA) were measured at baseline (t0) and once weekly for the remainder of the study (t1–t4). The leptin – body fat ratio was reduced during the two-week period of weight loss (t0 = 0.036 ± 0.016 (ng·mL–1)·g–1 vs. t1 = 0.010 ± 0.004 (ng·mL–1)·g–1 and t2 = 0.015 ± 0.007 (ng·mL–1)·g–1, p < 0.05). Leptin concentration plateaued at its nadir (~0.24 (ng·mL–1)·g–1) at fat mass < 22 g. IGF-1 was correlated with lean mass (r = 0.45, p < 0.05) and fat mass (r = 0.58, p < 0.05), regardless of body mass. We concluded that the leptin – body fat ratio was reduced early in food restriction and the correlation between these two variables was absent at low body fat.

Sign in / Sign up

Export Citation Format

Share Document