A Deficit in preprodynorphin mRNA expression in hippocampus of spontaneously hypertensive rats

1994 ◽  
Vol 23 (4) ◽  
pp. 345-348 ◽  
Author(s):  
John Q. Wang ◽  
Sijia Li ◽  
Alphonse J. Ingenito ◽  
Jacqueline F. McGinty
Keyword(s):  
Mrna Expression ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

scholarly journals Comparison of BASP1 and MARCKS protein exchange traits in the kidneys of rats with spontaneous

2020 ◽  
pp. 19-26
Author(s):  
Анна Сергеевна Альдекеева
Keyword(s):  
Mrna Expression ◽  
Spontaneously Hypertensive Rats ◽  
Cortical Layers ◽  
Hypertensive Rats ◽  
Tissue Samples ◽  
Spontaneously Hypertensive ◽  
Expression Levels ◽  
Interlinear Differences ◽  
Mrna Expression Levels ◽  
Marcks Protein

Исследовали возможные различия в обмене белков BASP1 и MARCKS в почках крыс со спонтанной гипертензией (линияSHR), путем сравнения уровней экспрессии мРНК этих белков в различных структурах почек. Работа выполнена на самцах крыс линии SHR (n=8) и крыс линии WKY (n=8), в возрасте 90 дней. Исследование проводили на образцах ткани из коркового и мозгового слоев почек. Уровни экспрессии мРНК BASP1 и MARCKS определяли методом ПЦР в реальном времени. Обнаружены достоверные различия (p<0.001) уровней экспрессии мРНК BASP1 и MARCKS между корковыми и мозговыми слоями почек у крыс линий SHR и WKY. При этом межлинейные различия в уровнях экспрессии мРНК обоих белков практически отсутствовали (p=0.050). Показано, что неравномерное распределение уровней экспрессии мРНКбелков BASP1 и MARCKS в мозговом и корковом слоях почек не зависит от уровня артериального давления и наличия спонтанной гипертензии This study aims to detect differences in BASP1 and MARCKS protein metabolisms in kidneys of spontaneously hypertensive rats by comparing their mRNA expression in different kidney structures. The work was performed on 90 days old male SHR (n = 8) and WKY (n = 8) rats. The study was carried out on tissue samples from cortical and medullar kidney layers. BASP1 and MARCKS mRNA expression levels were determined by real-time PCR. Significant differences (p <0.001) in the expression levels of BASP1 and MARCKS mRNA between cortical and medullar kidney layers in both SHR and WKY strain rats were found. Moreover, interlinear differences in the levels of both proteins’ mRNA expression were practically absent (p = 0.050). It was shown that uneven distribution of mRNA expression levels of BASP1 and MARCKS mRNA in kidneys medullar and cortical layers does not depend on the level of blood pressure and the presence of spontaneous hypertension.

scholarly journals THE EFFECTS OF LEPTIN ADMINISTRATION ON RENAL FUNCTION IN SPONTANEOUSLY HYPERTENSIVE RATS

2016 ◽  
Vol 10 (1) ◽  
pp. 108
Author(s):  
Brinnell Annette Caszo ◽  
Azdayanti Muslim ◽  
Zanariah Awang ◽  
Effat Omar ◽  
Effendi Ibrahim ◽  
...  
Keyword(s):  
Renal Function ◽  
Growth Factor ◽  
Mrna Expression ◽  
Spontaneously Hypertensive Rats ◽  
Transforming Growth Factor ◽  
Morphological Changes ◽  
Transforming Growth Factor Β1 ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Tgf Β1

ABSTRACTObjective: Elevated levels of leptin may be responsible directly for progression and severity of renal disease in obesity and hypertension. It may exertits effects by promoting fibrosis through the actions of transforming growth factor-β1 (TGF-β1) and the Smad pathway. This study determines theeffect of leptin administration on the development of renal fibrosis in nonobese spontaneously hypertensive rats (SHRs).Methods: Male SHRs, aged 12-14 weeks, were injected with either leptin (60 µg/kg/day) or saline (for the control group) subcutaneously daily for42 days. At the end of the experimental period, animals were euthanized and their kidneys were removed. The right kidney was harvested for thedetermination of messenger ribonucleic acid (mRNA) expression of TGF-β1, Smad2, Smad3, and bone morphogenic protein 7 (BMP7). The left kidneyswere stored in neutral buffered 10% formalin until they were processed and stained with hematoxylin and eosin. Prepared slides were examinedunder light microscopy. 30 consecutive glomeruli were examined for the cell counts based on the number of nuclei seen and the total area of glomeruli.Results: No significant difference was evident in renal function between control and leptin-treated rats. Cellularity and area of glomeruli were also notdifferent between the two groups. mRNA expression of TGF-β1, Smad2, and BMP7 were, however, higher in leptin-treated rats.Conclusion: It appears that 6 weeks of leptin administration increases renal TGF-β1 and Smad2 levels but with little morphological changes in thekidney. Whether the elevated BMP7 expression was responsible for lack of effect of leptin on renal morphological changes remains unclear.Keywords: Leptin, Renal function, Hypertension, Glomerulus, Transforming growth factor-β1, Smad, Spontaneously hypertensive rats.

scholarly journals Heparin suppresses endothelin-1 peptide and mRNA expression in cultured endothelial cells of spontaneously hypertensive rats.

1994 ◽  
Vol 4 (9) ◽  
pp. 1683-1689
Author(s):  
K Yokokawa ◽  
M Kohno ◽  
A K Mandal ◽  
H Tahara ◽  
M Yanagisawa ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Mrna Expression ◽  
Spontaneously Hypertensive Rats ◽  
Actinomycin D ◽  
Mrna Level ◽  
Transcriptional Level ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Endothelin 1 ◽  
Wistar Kyoto

Heparin given sc consistently lowers blood pressure in spontaneously hypertensive rats (SHR). This study was designed to examine heparin's effect on vasoconstrictor endothelin-1 production in cultured aortic endothelial cells (EC). Aortic EC from SHR and normotensive Wistar Kyoto rats (WKY) were cultured and incubated with or without different concentrations of heparin. Heparin suppressed endothelin-1 release and endothelin-1 mRNA expression in a dose- and a time-dependent fashion in both WKY and SHR. The suppressive effects were more augmented in SHR than in WKY: SHR versus WKY--endothelin-1 level at 6 h = 8 +/- 1.8 versus 14 +/- 2.2 pg/10(6) cells (P < 0.01) and mRNA expression--85 versus 52% maximal inhibition by heparin, 10 U/mL (=70 micrograms/mL) (P < 0.01). When heparin was added with transcriptional inhibitor actinomycin D and incubated for 30 min, no further inhibition of endothelin-1 mRNA level measured after another 30 minutes was observed compared with the endothelin-1 mRNA level in cultured EC of SHR treated with just actinomycin D at 30 min. These results suggest that heparin regulates endogenous endothelin-1 production by cultured EC, probably at the transcriptional level, and that this effect is more marked in SHR than in WKY.

scholarly journals Honey Supplementation in Spontaneously Hypertensive Rats Elicits Antihypertensive Effect via Amelioration of Renal Oxidative Stress

2012 ◽  
Vol 2012 ◽  
pp. 1-14 ◽  
Author(s):  
Omotayo O. Erejuwa ◽  
Siti A. Sulaiman ◽  
Mohd S. Ab Wahab ◽  
Kuttulebbai N. S. Sirajudeen ◽  
Salzihan Salleh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Blood Pressure ◽  
Mrna Expression ◽  
Spontaneously Hypertensive Rats ◽  
Antihypertensive Effect ◽  
Related Factor ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Elevated Systolic Blood Pressure ◽  
Wistar Kyoto

Oxidative stress is implicated in the pathogenesis and/or maintenance of elevated blood pressure in hypertension. This study investigated the effect of honey on elevated systolic blood pressure (SBP) in spontaneously hypertensive rats (SHR). It also evaluated the effect of honey on the amelioration of oxidative stress in the kidney of SHR as a possible mechanism of its antihypertensive effect. SHR and Wistar Kyoto (WKY) rats were randomly divided into 2 groups and administered distilled water or honey by oral gavage once daily for 12 weeks. The control SHR had significantly higher SBP and renal malondialdehyde (MDA) levels than did control WKY. The mRNA expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and glutathione S-transferase (GST) were significantly downregulated while total antioxidant status (TAS) and activities of GST and catalase (CAT) were higher in the kidney of control SHR. Honey supplementation significantly reduced SBP and MDA levels in SHR. Honey significantly reduced the activities of GST and CAT while it moderately but insignificantly upregulated the Nrf2 mRNA expression level in the kidney of SHR. These results indicate that Nrf2 expression is impaired in the kidney of SHR. Honey supplementation considerably reduces elevated SBP via amelioration of oxidative stress in the kidney of SHR.

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