Effect of indole butyric acid (IBA) on stem cuttings of Shorea leprosula

1995 ◽  
Vol 72 (2-3) ◽  
pp. 199-206 ◽  
Author(s):  
H. Aminah ◽  
J.McP. Dick ◽  
R.R.B. Leakey ◽  
J. Grace ◽  
R.I. Smith
2018 ◽  
Vol 12 (2) ◽  
pp. 491-499
Author(s):  
Aurea Portes Ferriani ◽  
Cícero Deschamps ◽  
Wanderlei Do Amaral ◽  
Luiz Everson da Silva

Native Piper species present significant repellent, antimicrobial, inseticidal, anti-tumor and anti-protozoal biological activities. Studies on new species can discover unpublished potentialities and vegetative propagation for the development of cultivation protocols and reduce the natural extraction. The objective of this study was to evaluate the rooting of P. arboreum, P. cernuum and P. diospyrifolium stem cuttings with different treatments containing doses of indole butyric acid (0, 500; 1,000; 1,500; 2,000 and 3,000 mg L-1). Branches were collected in the “Bom Jesus Biological Reserve”, Parana state, Brazil. The cuttings, with a length of 12 cm and average diameter of 6 mm, were placed in 53 cm3 plastic tubes with the commercial substrate Tropstrato HP® and intermittent misting. After 90 days, the survival, mortality, cuttings with new shoots, number and length of the three main roots were evaluated. The percentages of rooting reached an average of 67.5% for P. arboreum, 51.6% for P. cernuum and 50.4% for P. diospyrifolium. A positive effect in the treatments containing the plant regulator was observed for rooting development, percentage of cuttings with shoots and number of roots per propagule, but there were variations in the responses of each species. Leaf retention was an important factor for the adventitious rooting formation in all evaluated species.


2020 ◽  
Author(s):  
Javaid Iqbal ◽  
Bushra Khan ◽  
Sardar Khan ◽  
Nasreen Ghaffar ◽  
Ishaq Ahmad Mian ◽  
...  

Abstract Background Himalayan yew (Taxus wallichiana) is one of the endangered medicinal plants species having great importance due to the presence of anticancer drug Taxol. This metabolite is mainly used for the treatment of ovarian, breast, AIDS-related cancers, and other indications. The study being reported here was conducted for the propagation of Himalayan yew by using two different trials of Indole Butyric acid (IBA) and Indole acetic acid (IAA) hormones treatments (2000–7000 ppm) through stem cuttings (140 each). In the same way, 3840 cuttings were treated with IBA and IAA from November 2016 to November 2017 at Lalku valley, Swat, Khyber Pakhtunkhwa (KP), Pakistan. Results The influence of IBA treatment (7000 ppm) showed a survival of 85.22% (average number of roots = 10.4, average length of roots = 15.5 cm, average number of leaves = 92.4 and average number of sprouts = 3.3) while that of IAA treatment (7000 ppm) the survival of 81.11% (average number of roots = 9.1, average length of roots = 14.6 cm, average number of leaves = 84.0 and average number of sprout = 3.0) were more significant followed by 2000–6000 ppm (IBA and IAA). Lowest survival 40–45% (average number of roots = 4.2, average length of roots = 8.0 cm, average number of leaves = 32.2, average number of sprouts = 1.7) was noticed for controlled cuttings. Conclusion The present study enhanced the potential of conservation and propagation of T. wallichiana. Hence our study suggests and recommends the application of IBA (7000 ppm) as a better hormone for the conservation and propagation of Himalayan yew.


2017 ◽  
Vol 47 (1) ◽  
Author(s):  
Rosimeri de Oliveira Fragoso ◽  
Carlos André Stuepp ◽  
Helena Cristina Rickli ◽  
Katia Christina Zuffellato-Ribas ◽  
Henrique Soares Koehler

ABSTRACT: Prunus serrulata is a species widely used in the ornamentation and its economic importance is mainly due to the time of its flowering. The objective was to assess the feasibility of cutting technique for propagating P. serrulata , using stem cuttings from current-year shoots, and to estimate the maximum efficiency concentration of auxin indole butyric acid (IBA), in the promotion of rooting. Collection of plant material took place in late spring. Cuttings were made with about 8±1cm length and two leaves reduced to 50% of its original surface. Twenty one IBA concentrations have been assessed (0, 250, 500, 750, 1000, 1250, 1500, 1750, 2000, 2250, 2500, 2750, 3000, 3250, 3500, 3750, 4000, 4250, 4500, 4750 e 5000mg L-1), applied in base of cuttings, which were placed in acclimatized greenhouse. After 60 days, there was a gradual increase in the rooting percentage compared to the increment of IBA to the approximate concentration of 2700mg L-1, with results higher than 80%. In addition, low efficiency concentrations of growth regulators have resulted in fewer roots per cutting, which could compromise the survivability of the seedlings under ambient conditions. Conversely, the decrease in the percentage of callus may be an indication that root formation in P. serrulata occurs directly, without requiring callus formation and its further de-differentiation and re-differentiation in roots.


Author(s):  
Sina Kordzadeh ◽  
Hassan Sarikhani

The rootstock GF677 is an interspecific hybrid with an important economic and horticultural value. In this research, the effect of indole butyric acid (IBA) in combination with putrescine (Put) and hydrogen peroxide (H2O2) on rooting of GF677 semi-hardwood stem cuttings in three cutting seasons (July, March and October) was investigated. Treatments as IBA (0, 1000, 2000 and 3000 mg L-1), Put (0, 800, 1600 and 3200 mg L-1) and H2O2 (1.5, 3 and 6% w/v) were included. The results showed that in July cuttings, the highest levels of callogenesis were observed in IBA treated cuttings in both concentrations of 1000 and 2000 mg L-1. The rooting was very low in July cuttings, while the highest percentage of rooting (14%) was observed in the combination of 2000 mg L-1 IBA+ 3% H2O2. In March, the cuttings treated by 1000 mg L-1 IBA+800 mg L-1 Put and 1000 mg L-1 IBA+1600 mg L-1 Put revealed the highest percentages of callus formation 83.31 and 83.33%, respectively. In these cuttings, the highest percentage of rooting (63.88%) was gained at 2000 mg L-1 IBA+3200 mg L-1 Put. The application of 1000 mg L-1 IBA+800 mg L-1 Put increased root fresh weight. In cuttings prepared in October, only 800 mg L-1 Put caused callus formation in more than 55% of the cuttings. The rooting of cuttings at this time was as low as the July cuttings, whereas the highest rooting percentage was observed in cuttings treated with IBA at a concentration of 1000 mg L-1. Overall, the experiment showed that the season of the cutting and the treatments with IBA+Put or H2O2 could improve rooting properties of the rootstock GF677.


2017 ◽  
Vol 14 (2) ◽  
pp. 124-129 ◽  
Author(s):  
Mohammed Enamul Hoque

A field experiment was conducted at the central nursery of tea extension center of Bangladesh Tea Board in Bandarban during September to November, 2015 to determine the precise concentration of Indole Butyric Acid (IBA) on propagation through stem cuttings of tea. The experiment was laid out in a randomized block design with four treatments of IBA concentrations: 0 ppm (Control), 2000 ppm, 4000 ppm and 6000 ppm and each treatment was replicated four times. Basal part of tea (BT-2 variety) cuttings were dipped in different IBA concentrations for five seconds. Then these cuttings were dibbled into prepared soil mixture as rooting media. Cuttings were exposed to the rooting media for 90 days and then they all were removed carefully to assess the variation of establishment of cuttings. IBA treated cuttings showed better effect on the rooting rate, root length, root number, shoot length and survival rate compared to the control. It was noted that cuttings treated with 4000 ppm IBA produced the highest rooting rate 79.85%, the highest survival rate 57.60% and the highest root length of 4.85 cm followed by treatment 6000 ppm IBA. In case of all parameters, effects of the treatments 4000 ppm and 6000 ppm of IBA were statistically similar. Positive and strong correlation was observed both in rooting rate versus survival rate and root length versus shoot length in IBA treated cuttings. Thus, IBA at 4000 ppm concentration was recommended for establishment of stem cuttings of tea (BT-2 variety) in the nursery.The Agriculturists 2016; 14(2) 124-129


2020 ◽  
Vol 12 (3) ◽  
pp. 260-263
Author(s):  
A. Saeed ◽  
T. Amin

Abstract. The research was conducted in 2018 in order to investigate the effect of location, tree gender and indole butyric acid (IBA) 4000ppm on rooting of semi-hardwood stem cuttings. Two locations (Al-Samrah and Al-Nabaeen) in Latakia province, Syria, different in latitude and above sea level (500 m and 827 m, respectively) were studied. The results showed significant effect (p<0.05) of the location and tree gender on rooting capacity. No rooting was observed in control (without IBA treatment). Location also affected the number of roots/cuttings. No effect of location or tree gender on root length and diameter was established. The highest rooting rates (%) were for cuttings taken from female trees from Al-Samrah location when treated with IBA 4000 ppm (82.2%).


2018 ◽  
Vol 8 (4) ◽  
pp. 587-595 ◽  
Author(s):  
André Renato Rinaldi ◽  
Fabíola Villa ◽  
Daniel Fernandes da Silva ◽  
Rafael Massahiro Yassue

The objective of this study was to evaluate the asexual propagation of Dovyalis, through the use of substrates and cuttings. Three experiments were conducted in Marechal Cândido Rondon, Paraná State, Brazil, and the first experiment consisted of three removal positions of the cuttings (apical, middle and basal) x 4 Indole butyric acid (IBA) concentrations (0 mg L-1, 1000 mg L-1, 2000 mg L-1 and 3000 mg L-1); the second experiment evaluated the number of leaves in the cuttings (2, 4 and 6) x 3 cutting sizes (10, 15 and 20 cm); and the third experiment evaluated four substrates for rooting. The experiment was carried out in a randomized blocks design, with the first in a 3x4 and the second in a 3x3 factorial, respectively, containing 4 repetitions of 15 cuttings. The third experiment consisted of 5 repetitions of 15 cuttings. After the IBA treatment, the cuttings were taken to rooting in sandy beds during 70 days. The agronomical variables were evaluated. Basal and middle cuttings resulted in root systems with better development. The use of 1666 mg L-1 of IBA favors the Dovyalis cuttings rooting. Cuttings with 15 and 20 cm with four leaves favors the Dovyalis vegetative propagation. Dovyalis cuttings develop better on substrates containing latosol and vermiculite.


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