Production of food nanomaterials by specialized equipment

The paper describes how to determine the noise in a production hall where there are production machines and equipment. The chosen plant is a production unit where filling of the polyurethane foam tubes is performed. The main activity is the production of professional insulators and wholesale of other products from the same range enumerating the following: wholesale chemical products; production of professional insulators; trade in chemicals and chemical industry. The recommended maximum admissible value for a normal 8h work program is 85 dB (A). The objective is to determine the level of noise in the factory and how it acts as a physical professional risk factor and its effects on the human body. The way in which the proposed objective was achieved was to determine the value of the noise level with the specialized equipment. Following the determinations that will be presented in the following chapters, appropriate protective measures have been taken.

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Diagnostics of the location of damage to tubing oil well pipes

Tyumen State University Herald Physical and Mathematical Modeling Oil Gas Energy ◽

10.21684/2411-7978-2019-5-4-115-128 ◽

2019 ◽

Vol 5 (4) ◽

pp. 115-128

Author(s):

Timur A. Ishmuratov ◽

Rif G. Sultanov ◽

Milyausha N. Khusnutdinova

Keyword(s):

Fluid Motion ◽

Oil Well ◽

General Belief ◽

Oil Flow ◽

Specialized Equipment ◽

Improving Accuracy ◽

Analytical Expressions ◽

Technical Measures ◽

Quick Determination

The study is devoted to the mathematical description of the process of oil outflow in places of leakage of the tubing string, which allows a computer to locate a leakage in the tubing. The authors propose methodology for identifying defects in the tubing and determining the location of the leak. The uniqueness of this methodology lies in quick determination of the place of leakage without the use of specialists, sophisticated and specialized equipment. Mathematical modeling of oil flow in the tubing requires the apparatus of continuum mechanics. It is a general belief that the movement of oil in the pipe flows at low speeds due to its outflow from the hole. Using the general equations of mass and energy balance, the authors have obtained differential equations of fluid motion in a vertical pipe in the process of its outflow from the tubing and in the process of injection. Analytical expressions are the solution to these equations, as they can help in estimating the degree of damage and its location, as well as the feasibility of its eliminating. The results show that an increase in the leakage and injection times leads to improving accuracy of locating damage. Thus, when conducting various geological and technical measures (GTM) at the well, it is possible to assess the presence of leakage and its intensity when deciding on the repair of tubing.

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Copper Catalysts in the Synthesis of Five-membered N-polyheterocycles

Current Organic Synthesis ◽

10.2174/1570179415666180815144442 ◽

2018 ◽

Vol 15 (7) ◽

pp. 940-971 ◽

Cited By ~ 9

Author(s):

Navjeet Kaur

Keyword(s):

Transition Metal ◽

Organic Synthesis ◽

Copper Catalysts ◽

Research Field ◽

Chemoselective Synthesis ◽

Specialized Equipment ◽

Transition Metal Catalyzed ◽

Synthetic Methodologies ◽

Metal Catalyzed ◽

Traditional Approaches

Background: Due to significant biological activity associated with N-, O- and S-heterocycles, a number of reports for their synthesis have appeared in recent decades. Traditional approaches require expensive or highly specialized equipment or would be of limited use to the synthetic organic chemist due to their highly inconvenient approaches. This review summarizes the applications of copper catalysts with the emphasis on their synthetic applications for nitrogen bearing polyheterocylces. In summary, this review article describes the synthesis of a number of five-membered poly heterocyclic rings. Objective: Nowadays new approaches that employ atom-economical and efficient pathway have been developed. The researchers are following natural models to design and synthesize heterocycles. The transition metal catalyzed protocols have attracted the attention as compared to other synthetic methodologies because they use easily available substrates to build multiple substituted complicated molecules directly under mild conditions. In organic synthesis, constituted by transition metal catalyzed coupling transformations are one of the most powerful and useful protocols. The N-heterocycles are synthesized by this convenient and useful tool. Conclusion: The efficient and chemoselective synthesis of heterocycles by this technique has appeared as an important tool. This review shows a highly dynamic research field and the employment of copper catalysts in organic synthesis. Several strategies have been pointed out in the past few years, to meet more sustainable, efficient and environmentally benign chemical products and procedures. The catalytic strategies have been the focus of intense research because they avoid the use of toxic reagents. Among these catalytic strategies, highly rewarding and an important method in heterocycles synthesis is metal catalyzed synthesis.

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NeuroTec Sitem-Insel Bern: Closing the Last Mile in Neurology

Clinical and Translational Neuroscience ◽

10.3390/ctn5020013 ◽

2021 ◽

Vol 5 (2) ◽

pp. 13

Author(s):

Kaspar A. Schindler ◽

Tobias Nef ◽

Maxime O. Baud ◽

Athina Tzovara ◽

Gürkan Yilmaz ◽

...

Keyword(s):

Diagnostic Tests ◽

Current Model ◽

Disease Process ◽

Therapeutic Interventions ◽

Sleep Studies ◽

Last Mile ◽

Specialized Equipment ◽

The One ◽

Treatment Procedures ◽

Digital Biomarkers

Neurology is focused on a model where patients receive their care through repeated visits to clinics and doctor’s offices. Diagnostic tests often require expensive and specialized equipment that are only available in clinics. However, this current model has significant drawbacks. First, diagnostic tests, such as daytime EEG and sleep studies, occur under artificial conditions in the clinic, which may mask or wrongly emphasize clinically important features. Second, early detection and high-quality management of chronic neurological disorders require repeat measurements to accurately capture the dynamics of the disease process, which is impractical to execute in the clinic for economical and logistical reasons. Third, clinic visits remain inaccessible to many patients due to geographical and economical circumstances. Fourth, global disruptions to daily life, such as the one caused by COVID-19, can seriously harm patients if access to in-person clinical visits for diagnostic and treatment purposes is throttled. Thus, translating diagnostic and treatment procedures to patients’ homes will convey multiple substantial benefits and has the potential to substantially improve clinical outcomes while reducing cost. NeuroTec was founded to accelerate the re-imagining of neurology and to promote the convergence of technological, scientific, medical and societal processes. The goal is to identify and validate new digital biomarkers that can close the last mile in neurology by enabling the translation of personalized diagnostics and therapeutic interventions from the clinic to the patient’s home.

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Sample preparation for laser-microdissection of soybean shoot apical meristem

International Journal of Plant Biology ◽

10.4081/pb.2012.e3 ◽

2012 ◽

Vol 3 (1) ◽

pp. 3 ◽

Cited By ~ 1

Author(s):

Chui E. Wong ◽

Mohan B. Singh ◽

Prem L. Bhalla

Keyword(s):

Sample Preparation ◽

Shoot Apical Meristem ◽

Apical Meristem ◽

Laser Microdissection ◽

Specific Cell ◽

Legume Crop ◽

Continuous Formation ◽

Molecular Events ◽

Depth Analysis ◽

Specialized Equipment

The shoot apical meristem houses stem cells responsible for the continuous formation of aerial plant organs including leaves and stems throughout the life of plants. Laser-microdissection in combination with high-throughput technology such as next generation sequencing permits an in-depth analysis of molecular events associated with specific cell type of interest. Sample preparation is the most critical step in ensuring good quality RNA to be extracted from samples following laser-microdissection. Here, we optimized the sample preparation for a major legume crop, soybean. We used Farmer’s solution as a fixative and paraffin as the embedding medium for soybean shoot apical meristem tissue without the use of any specialized equipment. Shorter time for tissue fixation (two days) was found to be critical for the preservation of RNA in soybean shoot apical meristem. We further demonstrated the utility of this method for different tissues derived from soybean and rice. The method outlined here shall facilitate studies on crop plants involving laser-microdissection.

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Multi-Layer Construction Process for Fabricating Electrospun Fiber Embedded Microfluidic Devices

Volume 3: Biomedical and Biotechnology Engineering ◽

10.1115/imece2015-52086 ◽

2015 ◽

Author(s):

Karen Chang Yan ◽

John Sperduto ◽

Michael Rossini ◽

Michael Sebok

Keyword(s):

Biomedical Applications ◽

Electrospun Fiber ◽

Microfluidic Devices ◽

Construction Process ◽

Preliminary Results ◽

Device Optimization ◽

Specialized Equipment ◽

Microfabrication Techniques

Microfluidic devices are widely used in biomedical applications owing to their inherent advantages. Microfabrication techniques are common methods for fabricating microfluidic devices, which require specialized equipment. This paper presents a multi-layer construction process for producing microfluidic devices via integrating two accessible fabrication techniques — hydrogel molding, a microfabrication-free method, and electrospinning (ES). The formed microchannels were examined via analyzing micrographs. Preliminary results demonstrate the feasibility of the method and potential for incorporating complex channels and device optimization.

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A Rapid Bacteriophage DNA Extraction Method

Methods and Protocols ◽

10.3390/mps1030027 ◽

2018 ◽

Vol 1 (3) ◽

pp. 27 ◽

Cited By ~ 2

Author(s):

Džiuginta Jakočiūnė ◽

Arshnee Moodley

Keyword(s):

Dna Extraction ◽

Illumina Miseq ◽

Extraction Methods ◽

Resistant Bacteria ◽

Simple Method ◽

Antibiotic Resistant ◽

Phage Dna ◽

Specialized Equipment ◽

Miseq Platform ◽

Dna Extraction Methods

Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria. As part of gaining a better understanding of phages and to determine if phages harbor putative virulence factors, whole genome sequencing is used, for which good quality phage DNA is needed. Traditional phage DNA extraction methods are tedious and time consuming, requiring specialized equipment e.g., an ultra-centrifuge. Here, we describe a quick and simple method (under four hours) to extract DNA from double stranded DNA (dsDNA) phages at titers above 1.0 × 1010 plaque-forming units (PFU)/mL. This DNA was suitable for library preparation using the Nextera XT kit and sequencing on the Illumina MiSeq platform.

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Development of an ERIC sequence typing scheme for Laribacter hongkongensis, an emerging pathogen associated with community-acquired gastroenteritis and travellers’ diarrhoea

Journal of Medical Microbiology ◽

10.1099/jmm.0.049858-0 ◽

2013 ◽

Vol 62 (5) ◽

pp. 701-707 ◽

Cited By ~ 7

Author(s):

Jia-Li Feng ◽

Jin-Yan Lin ◽

Xiao-Bing Jiang ◽

Ou Zhang ◽

Jiang-Feng Zhu ◽

...

Keyword(s):

Clustering Analysis ◽

Consensus Sequence ◽

Pulsed Field Gel Electrophoresis ◽

Epidemiological Investigation ◽

Enterobacterial Repetitive Intergenic Consensus ◽

Emerging Pathogen ◽

Outbreak Investigations ◽

Laribacter Hongkongensis ◽

Specialized Equipment ◽

Typing Methods

Laribacter hongkongensis is a potential emerging pathogen, associated with community-acquired diarrhoea. For epidemiological purposes, different molecular typing methods, such as pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing, have been developed for this pathogen. However, these methods require specialized equipment and costly reagents. More importantly, they are labour-intensive and time-consuming, which is not really suitable for foodborne disease outbreak investigations. In this study, we developed a rapid and reliable method using 22-mer primers specific for the enterobacterial repetitive intergenic consensus sequence (ERIC). PFGE was used for comparison, to evaluate this method. A total of 81 isolates of L. hongkongensis were examined: 79 isolates recovered from food of diverse origins and two strains derived from patients with L. hongkongensis-associated infection. Typing patterns and clustering analysis indicated that the 81 L. hongkongensis isolates were grouped into 21 and 13 genotypes by ERIC-PCR and PFGE, respectively. ERIC-PCR was found as reproducible as PFGE. A high percentage (70.4 %) of isolates yielded distinguishable ERIC-PCR patterns, which were concordant with the results from PFGE. These results suggest that ERIC-PCR is valuable for use in the epidemiological investigation of L. hongkongensis.

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Immune-Mediated Neutropenia in a Miniature Poodle

Journal of the American Animal Hospital Association ◽

10.5326/jaaha-ms-6832 ◽

2019 ◽

Vol 55 (1) ◽

Author(s):

Benjamin Brunson

Keyword(s):

Bone Marrow ◽

Bone Marrow Aspirate ◽

Clinical Signs ◽

Response To Therapy ◽

Tertiary Referral Center ◽

Immune Mediated ◽

Specialized Equipment ◽

History Of ◽

Light Sedation ◽

The Right

ABSTRACT A 10 yr old spayed female toy poodle was presented to a tertiary referral center for a 10 day history of waxing and waning lethargy, vomiting, diarrhea, and anorexia. An immune-mediated neutropenia (IMN) was suspected to be the underlying cause of her clinical signs. A bone marrow aspirate was obtained from the chostochondral junction of the 11th and 12th ribs on the right side and provided a definitive diagnosis of IMN. A positive response to therapy and repeat blood work further confirmed the diagnosis. Obtaining bone marrow aspirates from the chostochondral junction is a safe, cheap, and reliable method of diagnosing IMN and can be performed in the private practice setting with light sedation and minimal need for specialized equipment.

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Cost-Saving Early Diagnosis of Functional Pain in Nonmalignant Pain: A Noninferiority Study of Diagnostic Accuracy

Pain Research and Treatment ◽

10.1155/2016/5964250 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

Rafael J. A. Cámara ◽

Christian Merz ◽

Barbara Wegmann ◽

Stefanie Stauber ◽

Roland von Känel ◽

...

Keyword(s):

Early Diagnosis ◽

Cost Saving ◽

Diagnostic Accuracy ◽

Pain Perception ◽

Diagnostic Testing ◽

Roc Curves ◽

University Hospital ◽

Screening Tests ◽

Functional Pain ◽

Specialized Equipment

Objectives. We compared two index screening tests for early diagnosis of functional pain: pressure pain measurement by electronic diagnostic equipment, which is accurate but too specialized for primary health care, versus peg testing, which is cost-saving and more easily manageable but of unknown sensitivity and specificity. Early distinction of functional (altered pain perception; nervous sensitization) from neuropathic or nociceptive pain improves pain management. Methods. Clinicians blinded for the index screening tests assessed the reference standard of this noninferiority diagnostic accuracy study, namely, comprehensive medical history taking with all previous findings and treatment outcomes. All consenting patients referred to a university hospital for nonmalignant musculoskeletal pain participated. The main analysis compared the receiver operating characteristic (ROC) curves of both index screening tests. Results. The area under the ROC curve for peg testing was not inferior to that of electronic equipment: it was at least 95% as large for finger measures (two-sided p=0.038) and at least equally as large for ear measures (two-sided p=0.003). Conclusions. Routine diagnostic testing by peg, which is accessible for general practitioners, is at least as accurate as specialized equipment. This may shorten time-to-treatment in general practices, thereby improving the prognosis and quality of life.

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