Role of Microorganisms in Performance Optimization of Wastewater Treatment Plants

2021 ◽  
pp. 67-91
Author(s):  
Kriti Akansha ◽  
Sudipta Majumder ◽  
Modhurima Misra ◽  
Shashwati Ghosh Sachan
2020 ◽  
Vol 38 ◽  
pp. 101544 ◽  
Author(s):  
Alain Lesimple ◽  
Saad Y. Jasim ◽  
Daniel J. Johnson ◽  
Nidal Hilal

2008 ◽  
Vol 1 ◽  
pp. ASWR.S752 ◽  
Author(s):  
Lucía Arregui ◽  
María Linares ◽  
Blanca Pέrez-Uz ◽  
Almudena Guinea ◽  
Susana Serrano

The biological community in activated sludge wastewater plants is organized within this ecosystem as bioaggregates or flocs, in which the biotic component is embedded in a complex matrix comprised of extracellular polymeric substances mainly of microbial origin. The aim of this work is to study the role of different floc-associated ciliates commonly reported in wastewater treatment plants-crawling Euplotes and sessile Vorticella- in the formation of aggregates. Flocs, in experiments with ciliates and latex beads, showed more compactation and cohesion among particles than those in the absence of ciliates. Ciliates have been shown to contribute to floc formation through different mechanisms such as the active secretion of polymeric substances (extrusomes), their biological activities (movement and feeding strategies), or the cysts formation capacity of some species. Staining with lectins coupled to fluorescein showed that carbohydrate of the matrix contained glucose, manose, N-acetyl-glucosamine and galactose. Protein fraction revealed over the latex beads surfaces could probably be of bacterial origin, but nucleic acids represented an important fraction of the extracellular polymeric substances of ciliate origin.


2020 ◽  
Vol 223 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Irene Bueno ◽  
Claudio Verdugo ◽  
Omar Jimenez-Lopez ◽  
Pedro Pablo Alvarez ◽  
Gerardo Gonzalez-Rocha ◽  
...  

1997 ◽  
Vol 36 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Tanja Gschlößl ◽  
Ingrid Michel ◽  
Marion Heiter ◽  
Christian Nerger ◽  
Verena Rehbein

In biological wastewater treatment inorganic and organic substances are fixed and metabolized by mixed populations of microorganisms forming either activated sludge flocs or biofilms. Not only the type of wastewater but also the operational conditions promote the development of an adapted biocenosis of microorganisms with specialized enzymatic functions. Understanding the biological properties of the microorganisms, it is possible to assess the prevailing conditions in their natural environment. Regular microscopic and enzymatic investigations of activated sludge and biofilms thus improve the assessment of the stability of the processes and support troubleshooting in wastewater treatment plants. While the role of bacteria is often discussed, the importance of ciliated protozoes and metazoes for the maintenance of the stability of biofilm systems is rarely mentioned. In this paper we intend to show some new results of direct microscopic observations in different sorts of biofilm systems focussing upon ciliated protozoes and metazoes. Practical results will demonstrate the relation between enzymatic analysis, microscopic investigations and performance of biofilm systems.


Water ◽  
2015 ◽  
Vol 7 (10) ◽  
pp. 5503-5524 ◽  
Author(s):  
Ramón Fuentes ◽  
Teresa Torregrosa ◽  
Enrique Ballenilla

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
S Bonetta ◽  
C Pignata ◽  
S a Bonetta ◽  
E Gasparro ◽  
E Lorenzi ◽  
...  

Abstract The global action plan on antimicrobial resistance reports the necessity to develop standards and guidance for the presence of antimicrobial agents in the environment, especially in wastewater, highlighting its possible role in the antibiotic resistance spreading. In addition, the New European One Health Action Plan against Antimicrobial Resistance underlines the need to support research into knowledge gaps on the release of resistant microorganisms into the environment and their dissemination. The aim of this study was to evaluate the presence of Antibiotic Resistance Bacteria (ARB) and Antibiotic Resistance Genes (ARG) in wastewater treatment plants (WWTPs). At this scope, untreated sewage and treated effluents of three different WWTPs (A, B and C) were sampled for one year. Sample dilutions were plated on R2Agar added/not-added with 4 different antibiotics (ampicillin 32mg/L; tetracycline 16 mg/L; chloramphenicol 32 mg/L; sulfamethoxazole 50,4 mg/L) to evaluate the percentage of antibiotic resistant bacteria and their WWTPs removal rate (%). DNA extraction on the filter used to concentrate the wastewater samples was performed to reveal the ARG presence; subsequently specific PCRs for ARG (blaTEM, tetA, sul II, sul III) were carried out. ARB were detected in all samples analysed. The highest antibiotic resistance percentage was revealed in the sewage (mean 21,7%±4,8) and effluent (mean 21,1%±3,0) of the three wastewater treatment plants for sulfamethoxazole. Moreover, sul II was the most present gene in the samples (81% of all samples, 89 % of sewages and 72% of effluents). The lower WWTPs removal was recovered in the plant B for the tetracycline (95, 7%). The results obtained underlines the need to monitor WWTP as critical hot spot for the antibiotic resistance spreading also considering the One Health approach. Furthermore, the results obtained could suggest interventions to reduce the spread of the antibiotic resistance in the integrated urban water cycle. Key messages The information obtained could provide usefulness information about the role of wastewater treatment plant in the antibiotic resistance spreading. The results could contribute to suggest the interventions targeted to reduce the antibiotic resistance phenomenon in the integrated urban water cycle.


Sign in / Sign up

Export Citation Format

Share Document