Endophytic fungi as a potential source of cytotoxic drugs: a fungal solution to cancer

AIM: The purpose of this research is to evaluate the antibacterial activity of endophytic fungi derived from the flowers of Andrographis paniculata (Sambiloto). METHODS: The endophytic fungi were obtained following the dilution method with potato dextrose agar as media. Four isolates of fungi have been obtained and then fermented with rice media for 3 weeks. The fermented fungi were extracted with ethyl acetate (EtOAc) and evaporated to yield the EtOAc extract. All EtOAc extracts were evaluated for their antibacterial activity using agar diffusion method. RESULTS: The results indicated that the EtOAc extract from fungus RG-2 was the potential source of antibacterial compounds. Molecular identification showing fungus RG-2 was Xylaria sp. CONCLUSION: Further investigation of the antibacterial compounds produced by fungus Xylaria sp. derived from the flowers of A. paniculata will be performed in the future. To the best of our knowledge, endophytic fungal Xylaria sp. is firstly isolated from A. paniculata.

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ENDOPHYTIC PESTALOTIOPSIS SPECIES FROM ANDAMAN ISLANDS: A POTENTIAL PANCREATIC LIPASE INHIBITOR

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i10.19795 ◽

2017 ◽

Vol 10 (10) ◽

pp. 82 ◽

Cited By ~ 1

Author(s):

Subha Jyoti Sarkar ◽

Divya Dioundi ◽

Mahiti Gupta

Keyword(s):

Endophytic Fungi ◽

Pancreatic Lipase ◽

Inhibitory Concentration ◽

Bioactive Compound ◽

Citrus Limon ◽

Andaman Islands ◽

Obesity Management ◽

Potential Source ◽

Cause Of Deaths ◽

Current Report

Objective: Obesity is the major cause of deaths worldwide. Inhibition of enzymes involved in breakdown of fats can be a good way for obesity management. Pancreatic lipase (PL) causes 50-70% degradation of ingested fats. The aim of this work is to explore the endophytic fungi from untapped biodiversity of Andaman Islands for PL inhibitors.Methods: In the current report, culture broths of 39 endophytic fungi from different curative plants of coastal regions of India have been tested for their potential PL inhibitory activity. The bioactive compound was thus isolated, purified, and analyzed using gas chromatography.Results: It was found that inhibitory concentration of a compound (Rf=0.64) isolated from crude hexane extract of endophytic fungal isolate from Citrus limon was 15.46 μg/ml. Gas chromatogram of the extract showed the presence of caryophyllene which might be responsible for the particular activity. The bioactive fungus was microscopically identified as Pestalotiopsis species.Conclusion: As caryophyllene is component of many oils and is non-toxic, so it can be potential source of safe and effective anti-obesity drug.

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The endophytic fungi of Salvia miltiorrhiza Bge.f. alba are a potential source of natural antioxidants

Botanical Studies ◽

10.1186/s40529-015-0086-6 ◽

2015 ◽

Vol 56 (1) ◽

Cited By ~ 11

Author(s):

Yan-Ling Li ◽

Xiao-Ming Xin ◽

Zheng-Yao Chang ◽

Ren-Jiu Shi ◽

Zeng-Min Miao ◽

...

Keyword(s):

Endophytic Fungi ◽

Salvia Miltiorrhiza ◽

Natural Antioxidants ◽

Potential Source

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Endophytic fungi from the medicinal herb Euphorbia geniculata as a potential source for bioactive metabolites

Archives of Microbiology ◽

10.1007/s00203-019-01740-x ◽

2019 ◽

Vol 202 (2) ◽

pp. 247-255 ◽

Cited By ~ 2

Author(s):

Noha M. Kamel ◽

Fatma F. Abdel-Motaal ◽

Soad A. El-Zayat

Keyword(s):

Endophytic Fungi ◽

Medicinal Herb ◽

Bioactive Metabolites ◽

Potential Source ◽

Euphorbia Geniculata

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How SIMS microscopy can be used in medicine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100132649 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1602-1603

Author(s):

Philippe Fragu

Keyword(s):

Mass Spectrometry ◽

Biomedical Applications ◽

Secondary Ion Mass Spectrometry ◽

Chemical Elements ◽

Biological Applications ◽

The Past ◽

Potential Source ◽

Secondary Ion ◽

Chemical Integrity ◽

Scientific Endeavour

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.

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