scholarly journals Antibacterial Screening of Endophytic Fungus Xylaria sp. derived from Andrographis paniculata (Sambiloto)

2021 ◽  
Vol 9 (A) ◽  
pp. 971-975
Author(s):  
Suryelita Suryelita ◽  
Riga Riga ◽  
Sri Benti Etika ◽  
Mariam Ulfah ◽  
Muh Ade Artasasta
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Potato Dextrose Agar ◽  
Andrographis Paniculata ◽  
Diffusion Method ◽  
Dilution Method ◽  
Etoac Extract ◽  
Antibacterial Compounds ◽  
Antibacterial Screening ◽  
Potential Source

AIM: The purpose of this research is to evaluate the antibacterial activity of endophytic fungi derived from the flowers of Andrographis paniculata (Sambiloto). METHODS: The endophytic fungi were obtained following the dilution method with potato dextrose agar as media. Four isolates of fungi have been obtained and then fermented with rice media for 3 weeks. The fermented fungi were extracted with ethyl acetate (EtOAc) and evaporated to yield the EtOAc extract. All EtOAc extracts were evaluated for their antibacterial activity using agar diffusion method. RESULTS: The results indicated that the EtOAc extract from fungus RG-2 was the potential source of antibacterial compounds. Molecular identification showing fungus RG-2 was Xylaria sp. CONCLUSION: Further investigation of the antibacterial compounds produced by fungus Xylaria sp. derived from the flowers of A. paniculata will be performed in the future. To the best of our knowledge, endophytic fungal Xylaria sp. is firstly isolated from A. paniculata.

scholarly journals MARINE SPONGE Aaptos suberitoides, IT’S POTENTIAL SOURCE OF NATURAL ANTIBACTERIAL FOR CONTROLLING Vibrio harveyi ON TIGER SHRIMP (Penaeus monodon) CULTURE

2015 ◽  
Vol 10 (1) ◽  
pp. 33
Author(s):  
Rosmiati Rosmiati ◽  
Andi Parenrengi ◽  
Emma Suryati
Keyword(s):  
Antibacterial Activity ◽  
Marine Sponge ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Shrimp Culture ◽  
Antibacterial Compounds ◽  
Potential Source ◽  
Tiger Shrimp

The study aims to isolate and identify the natural antibacterial compounds potential from Aaptos suberitoides for Vibrio harveyi control on tiger shrimp (Penaeus monodon) culture. The agar diffusion method using paper discs was used to determine the antibacterial activity of extracts (diethyl ether (DEE), butanol (BUE) and aqueous (HOE) and compounds successfully isolated against Vibrio harveyi. Findings showed that the antibacterial activity was concentrated in BUE with the inhibition zone of 17.2±0.1 mm. Meanwhile, two other extracts (DEE and HOE) did not exhibit any antibacterial activity against V. harveyi. From the active BUE, it was successfully isolated two compounds giving a strong anti-vibrio activity with the inhibition zone of 22±0.1 mm. The IR, 1H, 13C, COSY, HMQC, HMBC, and MS spectrum analysis indicated that both active compounds identified as aaptamine (1) and 9-demethyloxyaaptamine (2). The study suggested that marine sponge A. suberitoides may have potential compounds source for controlling of V. harveyi on tiger shrimp culture.

scholarly journals Microwave-Hydrothermal Rapid Synthesis of Cellulose/Ag Nanocomposites and Their Antibacterial Activity

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 978 ◽  
Author(s):  
Lian-Hua Fu ◽  
Qing-Long Gao ◽  
Chao Qi ◽  
Ming-Guo Ma ◽  
Jun-Feng Li
Keyword(s):  
Antibacterial Activity ◽  
Antimicrobial Properties ◽  
Silver Ions ◽  
Heating Time ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Human Beings ◽  
Microwave Hydrothermal ◽  
Wide Range

Silver-based antimicrobial nanomaterials are considered as the most promising antibacterial agents owing to their outstanding antimicrobial efficacy and their relatively low toxicity to human beings. In this work, we report on a facile and environment-friendly microwave-hydrothermal method to prepare cellulose/Ag nanocomposites using hemicellulose as the reductant. The influences of the microwave-hydrothermal heating time and temperature, as well as the hemicellulose concentration on the formation of cellulose nanocomposites, were investigated in detail. Experimental results indicated that the hemicellulose was an effective reductant for silver ions, with higher temperature and longer heating time favoring the formation of silver with higher crystallinity and mass content in the nanocomposites. Moreover, the antimicrobial properties of the as-prepared cellulose/Ag nanocomposites were explored using Gram-positive S. aureus ATCC 6538 and Gram-negative E. coli HB 101 by both disc diffusion method and agar dilution method, and the nanocomposites showed excellent antibacterial activity. These results demonstrate that the as-prepared cellulose/Ag nanocomposites, as a kind of antibacterial material, are promising for applications in a wide range of biomedical fields.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes

EKOLOGIA ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 31-39
Author(s):  
Oom Komala ◽  
. Ismanto ◽  
Muhammad Alan Maulana
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Dilution Method ◽  
Natural Treatment ◽  
Amomum Compactum

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.

Antibacterial Activity of Traditional Medicine Scurrula atropurpurea (BL) DANS and their Endophytic Fungi

2020 ◽  
Vol 840 ◽  
pp. 205-213
Author(s):  
Elfita Elfita ◽  
Muharni Muharni ◽  
Mardiyanto Mardiyanto ◽  
Fitrya Fitrya ◽  
Feti Fera ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Bacterial Infections ◽  
Salmonella Typhi ◽  
Spectroscopy Analysis ◽  
Chromatography Method ◽  
Antibacterial Compounds ◽  
Activity Test ◽  
Antibacterial Compound ◽  
Promising Source

Scurrula atropurpurea known as benalu is a medicinal plant that has been used for the treatment of various diseases such as antibacterial. Plants with ethnomedicine history use to cure pathogenic bacterial infections and their endophytic fungi is a promising source of antibacterial compounds. This study aimed to compared the antibacterial activity of S. atropurpurea leaves and their endophytic fungi. The secondary metabolites were isolated from the leaves of S. atropurpurea and their endophytic fungi by the chromatography method. The antibacterial activity test was carried out by Kirby Bauer method against Salmonella typhi (IPCCCB.11.669) and Escherichia coli (ATCC 25922) as Gram (-) and Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923) as Gram (+). The antibacterial compound from S. atropurpurea was determined by spectroscopy analysis as Quercetin-3-O-α-L-Rhamnopyranoside, while the antibacterial compound from endophytic fungi (strain BB1) as a lactone. Phylogenetic tree of strain BB1 has the highest homology with Neopestalotiopsis surinamensis strain CBS 450.74.

scholarly journals Antifungal Activity of Various Extracts of Seeds of the Plant Malva Parviflora (Linn)

2017 ◽  
Vol 14 (4) ◽  
pp. 1409-1412
Author(s):  
Jadhav Raina ◽  
Parihar Sangeeta
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antifungal Agent ◽  
Antifungal Agents ◽  
Diffusion Method ◽  
Dilution Method ◽  
Significant Activity ◽  
Water As Solvent ◽  
Malva Parviflora ◽  
Agar Well Diffusion Method

ABSTRACT: Development of more effective and less toxic antifungal agents is required for the treatment of various fungal disease.Plants and their extraction preparation have beenused as medicine against infectious diseases.The present study was aimed to study, the antifungal activity of the seeds of various seed abstract of plant Malva Parviflora (Linn). The antifungal activity of seeds extract of plant was determine by using agar well diffusion method,MIC(minimum inhibitoryconcentration) and MFC (minimum fungicidal count)by using micro dilution method . The seeds extract of the plant were examined using Methanol, Ethyl acetate ,Petroleum ether and water as solvent and tested against different fungi pathogens. From the result it can be concluded that the all the seeds extract shows the significant activity against the micro organism, hence these extract may be used as a source of antifungal agent obtained from herbal medicine and may be explore as new and effective antifungal agent. Various solvent extracts of the plant Malva parvifiora (Linn) have been found to possess enough antibacterial activity and may potentially be explored as human antifungal agent.

scholarly journals Uji Efek Antibakteri Jamur Endofit Akar Bakau Rhizophora stylosa Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Michele Sumampouw
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Potato Dextrose Agar ◽  
Rhizophora Stylosa ◽  
E Coli

Abstract: The aim of this study is to investigate the antibacterial effect of endophytic fungi originated from the root of mangrove Rhizophora stylosa growing on Mangrove Plantation Area around DAS Simpang 5 Jl. Piere Tendean Manado. The method of this research was using combination of PDA and NA media. Two species of endophytic fungi were extracted from the root of R. stylosa, black mycelium fungi as isolate A and brown mycelium fungi as isolate B. The activities of both isolates were tested against pathogenic bacteria Staphylococcus aureus and Escherichia coli. The result showed the same inhibition zone of isolate A and B to S. aureus, while, isolate A showed bigger the inhibition zone in comparison to isolate B against E. coli. As a conclusion, the endophytic fungi taken from the root of R. stylosa have antibacterial activity toward S. aureus and E. coli. Keywords: endophytic fungi, Rhizophora stylosa, antibacterial, Staphylococcus aureus, Escherichia coli.   Abstrak: Penelitian ini bertujuan untuk melihat adanya efek antibakteri dari jamur endofit yang diambil dari akar bakau Rhizophora stylosa yang ditanam di sekitar Perairan Daerah Aliran Sungai Simpang Lima Jl. Piere Tendean Manado. Metode penelitian dilakukan dengan menggunakan kombinasi media Potato Dextrose Agar (PDA) dan Nutrient Agar (NA). Dari akar bakau R. stylosa diperoleh dua  jenis jamur endofit yaitu isolat A dengan karateristik miselium jamur berwarna hitam dan isolat B yang dengan karakteristik miselium berwarna coklat. Kedua isolat ini selanjutnya diujikan aktivitasnya terhadap bakteri Staphylococcus aureus dan Escherichia coli. Hasil penelitian menunjukkan isolat A dan B memberikan  zona hambat yang sama terhadap bakteri Staphylococcus aureus dan pada bakteri Escherichia coli isolat A menunjukkan diameter zona hambat yang lebih besar dibandingkan isolat B. Kesimpulan, Jamur endofit yang diisolasi dari akar bakau R. Stylosa memiliki efek antibakteri terhadap pertumbuhan bakteri S. aureus dan E. coli. Kata kunci: jamur endofit, Rhizophopra stylosa, antibakteri, Staphylococcus aureus, Escherichia coli.

scholarly journals Antibacterial Activity of Hypogymnia Physodes Extract and its Combinations with Antibiotics Against Enterococci

2018 ◽  
pp. 52-56
Author(s):  
D. V. Tapalsky ◽  
K. M. Kosenkova
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Acetone Extract ◽  
Diffusion Method ◽  
Dilution Method ◽  
Synergistic Activity ◽  
Hypogymnia Physodes ◽  
Resistant Strains ◽  
Vancomycin Resistant ◽  
Promising Source

Objective : to study antibacterial properties of Hypogymnia physodes extract and its combinations with antibiotics against vancomycin-sensitive and vancomycin-resistant strains of enterococci. Material and methods. Minimum inhibitory concentrations (MICs) of the acetone extract of H. physodes for reference and clinical isolates of Enterococcus faecalis were determined by the serial broth dilution method. Evaluation of efficiency of the combined effect of the H. physodes extract in combination with antibiotics was carried out using the modified disc-diffusion method and «chessboard» method. Results. We have revealed marked antibacterial activity (MIC 32-64 mcg/ml) of the acetone extract of H. physodes against vancomycin-sensitive and vancomycin-resistant strains of enterococci and found a synergistic effect (ΣFIC from 0.125 to 0.375) of the combination of the H. physodes extract and aminoglycosides against E. faecalis , including vancomycin-resistant strains. Conclusion. H. physodes is a promising source of compounds with antibacterial activity, further research is required to identify and isolate a secondary metabolite having synergistic activity in combination with aminoglycosides from the lichen extract.

scholarly journals Eco-Friendly Cinnamaldehyde Based Emulsion for Phytopathogenic Bacterial Growth Inhibitor

2020 ◽  
pp. 1-12
Author(s):  
Tahany G. M. Mohammed ◽  
A. F. Abd El- Rahman
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Ralstonia Solanacearum ◽  
Erwinia Amylovora ◽  
Plant Protection ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agricultural Crop ◽  
Water Emulsion ◽  
Phytopathogenic Bacteria

The formulation plays an essential role in achieving the successful delivery and biological activity of any plant protection products. This study aimed to develop a cinnamaldehyde water-based formulation (oil-in-water emulsion) via a high-shear stirring emulsification method. Cinnamaldehyde emulsion was successfully prepared and characterized using different physicochemical parameters (emulsion stability, persistent foaming, accelerated storage at 54°C for 2 weeks, and stability at 0°Cfor one week, as well as pH, surface tension, flash point, viscosity, and particle size distribution). Also, the antibacterial activity was verified in vitro against some important phytopathogenic bacteria; Erwinia amylovora, Pectobacterium aroidearum, Pseudomonas aeruginosa, and Ralstonia solanacearum using well diffusion method. In addition, the minimum inhibition concentration (MIC) was determined by the twofold dilution method. The results revealed that the prepared formulation showed good storage stability, exhibited non-Newtonian shear-thinning behavior and promising antibacterial activity. The inhibition zones against the tested phytopathogenic bacteria were ranged from 10.3 mm to 52.0 mm. MICs of the prepared formulation were 15.63, 31.25, 62.5, and 15.63 μl/ml against Erwinia amylovora, Pectobacterium aroidearum, Pseudomonas aeruginosa and Ralstonia solanacearum, respectively. Our results provide an environmentally friendly formulation with promising activity to control the agricultural crop disease.

scholarly journals Screening of Antibacterial Activities of the Endophytic Fungi Isolated from the Leaves of Medinilla speciosa Blume

2021 ◽  
Vol 8 (3) ◽  
pp. 24-28
Author(s):  
Puteri Amelia ◽  
Rachma Ayunda ◽  
Saiful Bahri
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Bioactive Compound ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Shigella Dysenteriae ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Bacillus Subtilis Atcc

Endophytes or endophytic fungi have been investigated as a store house of bioactive compound. This study was aimed to evaluate the potential antibacterial activity of endophytic fungi isolated from Medinilla speciosa Blume. The antibacterial test was determined by measuring the inhibition zone with disk-diffusion method. Twenty endophytes were isolated from the leaves of Medinilla speciosa Blume and identified morphologically. The results demonstrated that ten isolates showed variation in their antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, and Shigella dysenteriae ATCC 13313. Further investigation will be needed to explore and identify the bioactive molecules of the isolated endophytic fungi.

scholarly journals ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL SENYAWA ANTIBAKTERI YANG BERASOSIASI DENGAN KARANG BATU DARI PERAIRAN BITUNG DAN SPONS DARI SELAT MAKASSAR

Jurnal BIOMA ◽  
2015 ◽  
Vol 11 (1) ◽  
pp. 42
Author(s):  
FRANSISCA SUNNY ◽  
TRI HANDAYANI KURNIATI ◽  
ARIANI HATMANTI
Keyword(s):  
Antibacterial Activity ◽  
Diffusion Method ◽  
Marine Agar ◽  
Antibacterial Compounds ◽  
Associated Bacteria ◽  
Antibacterial Assay ◽  
Sponge Associated Bacteria ◽  
Oxidase Test ◽  
El Tor ◽  
Produce Acid

ABSTRACT Recently the needs of antibacterial compounds is increasing. This is due to the bacterial resistence to common antibacterial  compounds.  coral  and  sponge-ssociated  bacteria  are  potential  producer of antibacterial compounds. This research was aim to obtain coral and sponge-associated bacteria that could produce antibacterial compound. coral associated-bacteria was isolated from Bitung and   was isolated in Marine Agar by pour plate method. The antibacterial compounds were obtained by extraction using ethyl acetate and acetone. The antibacterial assay was performed by agar diffusion method using paper discs and was performed by testing with  Staphylococcus  aureus,  Bacillus  subtilis, Vibrio cholerae biotipe El Tor, and Escherichia coli. Total 37 isolate  was  isolated  from  corals and 25 isolate from sponge obtained from Selat Makassar. Based on the assay, only bacteria from sponge that showed antibacterial activity. Two sponge-associated bacteria, S.5-8 and  S.2-1 NRBC were found to inhibit S. aureus. From those isolates, isolate S.5-8 produced bigger clear zone (2,6 mm) than S.2-1 NRBC (1,5mm). S.5-8 could hydrolize gelatine whereas S.2-1 NRBC showed positive result on oxidase test and was able to fement xilose and arabinose to produce acid.   Key words: antibacterial activity, association, characterization, coral, isolation, sponge

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