scholarly journals ENDOPHYTIC PESTALOTIOPSIS SPECIES FROM ANDAMAN ISLANDS: A POTENTIAL PANCREATIC LIPASE INHIBITOR

2017 ◽  
Vol 10 (10) ◽  
pp. 82 ◽  
Author(s):  
Subha Jyoti Sarkar ◽  
Divya Dioundi ◽  
Mahiti Gupta
Keyword(s):  
Endophytic Fungi ◽  
Pancreatic Lipase ◽  
Inhibitory Concentration ◽  
Bioactive Compound ◽  
Citrus Limon ◽  
Andaman Islands ◽  
Obesity Management ◽  
Potential Source ◽  
Cause Of Deaths ◽  
Current Report

  Objective: Obesity is the major cause of deaths worldwide. Inhibition of enzymes involved in breakdown of fats can be a good way for obesity management. Pancreatic lipase (PL) causes 50-70% degradation of ingested fats. The aim of this work is to explore the endophytic fungi from untapped biodiversity of Andaman Islands for PL inhibitors.Methods: In the current report, culture broths of 39 endophytic fungi from different curative plants of coastal regions of India have been tested for their potential PL inhibitory activity. The bioactive compound was thus isolated, purified, and analyzed using gas chromatography.Results: It was found that inhibitory concentration of a compound (Rf=0.64) isolated from crude hexane extract of endophytic fungal isolate from Citrus limon was 15.46 μg/ml. Gas chromatogram of the extract showed the presence of caryophyllene which might be responsible for the particular activity. The bioactive fungus was microscopically identified as Pestalotiopsis species.Conclusion: As caryophyllene is component of many oils and is non-toxic, so it can be potential source of safe and effective anti-obesity drug. 

scholarly journals Soy Bean‐Associated Endophytic Fungi as Potential Source for Anti‐COVID‐19 Metabolites supported by docking analysis

2021 ◽  
Author(s):  
Seham S. El‐hawary ◽  
Rabab Mohammed ◽  
Hebatallah S. Bahr ◽  
Eman Zekry Attia ◽  
Mo ‘men H. El‐Katatny ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Docking Analysis ◽  
Potential Source

scholarly journals Antimicrobial Activity of Leaves and Flowers of Cassia auriculata linn

1970 ◽  
Vol 46 (4) ◽  
pp. 513-518 ◽  
Author(s):  
V Subhadradevi ◽  
K Asokkumar ◽  
M Umamaheswari ◽  
AT Sivashanmugam ◽  
JR Ushanandhini ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Dominant Role ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Cassia Auriculata ◽  
Potential Source ◽  
Wide Range ◽  
Ancient Times

Since ancient times plant as sources of medicinal compounds have continued to play a dominant role in the maintenance of human health. To treat chronic and infectious diseases plants used in traditional medicine contain a wide range of ingredients. In this regard, Cassia auriculata L. (Caesalpiniaceae) is widely used in Ayurvedic medicine as a tonic, astringent and as a remedy for diabetes, conjunctivitis, ulcers, leprosy, skin and liver diseases. The aim of present study was to evaluate the antimicrobial activity of ethanolic extract of Cassia auriculata leaves and flowers (CALE & CAFE). CALE and CAFE exhibited broad spectrum antimicrobial activity against standard strains of Staphylococcus aureus, Escherichia coli and Bacillus subtilis and exhibited no antifungal activity against standard strains of Candida albicans and Aspergillus niger. Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) was carried out for CALE and CAFE. The results obtained in the present study indicate that the CALE and CAFE can be a potential source of natural antimicrobial agents. Key words: Cassia auriculata; Antimicrobial activity; Agar well diffusion method. DOI: http://dx.doi.org/10.3329/bjsir.v46i4.9600 BJSIR 2011; 46(4): 513-518

scholarly journals Isolation of Endophytic Fungi QPS 05 from Quercus phillyraeoides A. Gray and Its Potential for α-Glucosidase Inhibitory Activity

2018 ◽  
Vol 20 (1) ◽  
pp. 1-7
Author(s):  
Anastasia Wheni Indrianingsih ◽  
Amalia Indah Prihantini ◽  
Sanro Tachibana
Keyword(s):  
Fatty Acids ◽  
Endophytic Fungi ◽  
Inhibitory Activity ◽  
Endophytic Fungus ◽  
Inhibitory Concentration ◽  
Life Cycles ◽  
Ic50 Values ◽  
Quercus Phillyraeoides ◽  
Alternaria Sp ◽  
Harmful Effects

AbstractEndophytic fungi are the microorganisms that spend all or part of their life cycles within plant tissue without causing harmful effects on the plant. In this study, 14 endophytic fungus from Quercus phillyraeoides A. Gray were isolated. Alternaria sp. QPS 05, an endophytic fungi which was isolated from the stem of Q. phillyraeoides A. Gray showed the highest α-glucosidase inhibitory activity. Further separation of ethyl acetate extract from the fungus led to the isolation of active substance from hexane-soluble fraction which give fatty acids mixture consist of palmitic acid, oleic acid, linoleic acid and linolenic acid (1) strong inhibitory activity against α-glucosidase. Isolated fatty acids (1) had inhibitory concentration (IC50) values against Saccharomyces cerevisiae was 12.10 μg/mL. The results of the present study showed that endophytic fungus from Alternaria sp. QPS 05 potentially contained a rich source of natural antidiabetic medicine.

Bioactive potential of culturable fungal endophytes isolated from leaf of Catharanthus roseus (L.) G. Don

2021 ◽  
Vol 21 ◽  
Author(s):  
Sucheta Singh ◽  
Surjeet Verma ◽  
Dharmendra Kumar Yadav ◽  
Anant Kumar ◽  
Rekha Tyagi ◽  
...  
Keyword(s):  
Catharanthus Roseus ◽  
Crude Extract ◽  
Inhibitory Concentration ◽  
Efflux Pump ◽  
Fungal Endophytes ◽  
Bacterial Pathogen ◽  
Bioactive Compound ◽  
Major Constituent ◽  
Anti Inflammatory ◽  
Bioactive Potential

Introduction: Endophyte is considered as a source of natural bioactive secondary metabolites that provides an array of bioactive lead compounds. The present study was aimed to determine the antimicrobial and anti-inflammatory potential of fungal endophytes isolated from Catharanthus roseus. Methods: A total of seven fungal endophytes crude extract were screened against bacterial pathogens. Of these, Curvularia geniculata CATDLF7 crude extract exhibited the most potent inhibitory activity against bacterial pathogen. Hence, CATDLF7 crude extract was subjected to the chromatographic separation. This purification leads to the isolation of six pure compounds (1PS - 6PS). Of these, 3PS was found to be a major constituent and most effective against clinical isolates of methicillin resistant Staphylococcus aureus (MRSA) with minimum inhibitory concentration (MIC) values ranging from 100 to 200 μg/ml. Based on the spectroscopic data, 3PS was characterized as α,β-dehydrocurvularin. This compound also showed synergistic interaction with norfloxacin, and reduced its MIC up to 32-folds with fractional inhibitory concentration index (FICI) of 0.09. Results: To understand the possible antibacterial mechanism of action, α,β-dehydrocurvularin alone (100 μg/ml) exhibited efflux pump inhibitory potential by 0.84 fold decreasing in ethidium bromide (EtBr) fluorescence. In addition, α,β-dehydrocurvularin inhibited inflammatory cytokines TNF-α and IL-6 production which is further validated by molecular docking score -4.921 and -5.641 respectively for understanding orientation and binding affinity. Conclusion: Overall the results highlighted to identifying bioactive compound α,β-dehydrocurvularin which could be used as an antimicrobial and anti-inflammatory agents.

scholarly journals Chemical Composition and in Vitro Antimicrobial Activity of the Volatile Oils from Gliomastix murorum and Pichia guilliermondii, Two Endophytic Fungi in Paris polyphylla var. yunnanensis

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Jianglin Zhao ◽  
Tijiang Shan ◽  
Yongfu Huang ◽  
Xili Liu ◽  
Xiwu Gao ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Inhibitory Concentration ◽  
Octadecenoic Acid ◽  
Volatile Oil ◽  
Pichia Guilliermondii ◽  
Volatile Oils ◽  
Paris Polyphylla ◽  
Gliomastix Murorum

Volatile oils were obtained by hydrodistillation from Gliomastix murorum and Pichia guilliermondii, two endophytic fungi isolated from the traditional Chinese medicinal herb Paris polyphylla var. yunnanensis. The oils were analyzed for their chemical composition by gas chromatography-mass spectrometry (GC-MS). Palmitic acid (15.5%), (E)-9-octadecenoic acid (11.6%), 6-pentyl-5,6-dihydropyran-2-one (9.7%), and (7Z,10Z)-7,10- hexadecadienoic acid (8.3%) were the major compounds of the 40 identified components in G. murorum volatile oil. 1,1,3a,7-Tetramethyl-1a,2,3,3a,4,5,6,7b-octahydro-1H-cyclopropa[a]- naphthalene (25.9%), palmitic acid (15.5%), 1-methyl-2,4-di- (prop-1-en-2-yl)-1- vinylcyclohexane (7.9%), (E)-9-octadecenoic acid (7.3%), and (9E,12E)-ethyl-9,12-octadecadienoate (5.2%) were the major compounds of the 27 identified components in P. guilliermondii volatile oil. The in vitro antimicrobial activity of the volatile oils was also investigated to evaluate their efficacy against six bacteria and one phytopathogenic fungus. The minimum inhibitory concentration (MIC) values of the volatile oils against the test bacteria ranged from 0.20 mg/mL to 1.50 mg/mL. One of the most sensitive bacteria was Xanthomonas vesicatoria with an MIC of 0.20 mg/mL and 0.40 mg/mL for G. murorum and P. guilliermondii, respectively. The mean inhibitory concentration (IC50) of the volatile oils against spore germination of Magnaporthe oryzae was 0.84 mg/mL for G. murorum and 1.56 mg/mL for P. guilliermondii. These results indicated that the volatile oils from the endophytic fungi have strong antimicrobial activity and could be a potential source of antimicrobial ingredients.

scholarly journals Schinus terebinthifolius (Anacardiaceae) essential oil for minas frescal cheese preservation

2020 ◽  
Vol 9 (11) ◽  
pp. e1619119760
Author(s):  
Valquíria de Fátima Simões ◽  
Kátia Castilho de Oliveira ◽  
Wanessa de Campos Bortolucci ◽  
José Eduardo Gonçalves ◽  
Maria Graciela Iecher Faria ◽  
...  
Keyword(s):  
Essential Oil ◽  
Food Industry ◽  
Inhibitory Concentration ◽  
Chemical Compounds ◽  
Antimicrobial Effect ◽  
Schinus Terebinthifolius ◽  
Plant Essential Oils ◽  
Potential Source ◽  
Pepper Fruit ◽  
The Impact

Microorganism control in food has been a challenge due to the legislation restrictions of each country and the increase in the demand for food without chemical preservatives. Plant essential oils have antimicrobial action and are promising for the use in the food industry. Our study aimed to evaluate the chemical composition and the antimicrobial effect of Schinus terebinthifolius Raddi fruit essential oil on minas frescal cheese. The essential oil was extracted by hydrodistillation, and the chemical compounds were identified by gas chromatography coupled to mass spectrometry. The major identified compounds were hydrocarbon monoterpenes (57.0%), mainly: α-pinene (22.2%), limonene (17.0%), carvone (10.2%) and β-phellandrene (7.9%). The cheese was produced, the main microorganisms were isolated, and the minimum inhibitory concentration was determined through broth microdilution test.  The cheese samples were soaked. The pink pepper fruit essential oil was added to the cheese samples at the concentration of 20000 μL/mL superficially or to the micelle. S. terebinthifolius fruit essential oil was efficient to control minas frescal cheese microorganisms mainly when applied by superficial addition. The essential oil is a potential source of studies to develop applications for the control of microorganisms in cheese such as minas frescal. Further studies may exploit the impact of the essential oil in minas frescal cheese acceptability.

scholarly journals HPLC analysis, antimicrobial and antioxidant activities of Daphne cneorum L.

2012 ◽  
Vol 66 (5) ◽  
pp. 709-716 ◽  
Author(s):  
Nedeljko Manojlovic ◽  
Pavle Maskovic ◽  
Perica Vasiljevic ◽  
Ratomir Jelic ◽  
Marina Juskovic ◽  
...  
Keyword(s):  
High Performance ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Potential Source ◽  
Liquid Chromatographic

The present study describes in vitro antimicrobial and antioxidant activities of the methanol extracts which were obtained from the leaves and twigs of the plant Daphne cneorum L. The antimicrobial activity of these extracts was tested against human pathogenic microorganisms using a minimum inhibitory concentration (MIC) values. Total phenolics and flavonoids, as well as the antioxidant activity of the extracts were determined. The two tested extracts showed good antimicrobial and antioxidant activities. The results of a high performance liquid chromatographic (HPLC) method showed that 7,8-dihydroxycoumarin is one of the most abundant secondary metabolite in the tested extracts. The results of this study clearly indicated that the extracts of D. cneorum could be used as a potential source of natural antioxidant and antimicrobial agents.

scholarly journals Screening of Antibacterial Activities of the Endophytic Fungi Isolated from the Leaves of Medinilla speciosa Blume

2021 ◽  
Vol 8 (3) ◽  
pp. 24-28
Author(s):  
Puteri Amelia ◽  
Rachma Ayunda ◽  
Saiful Bahri
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Bioactive Compound ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Shigella Dysenteriae ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Bacillus Subtilis Atcc

Endophytes or endophytic fungi have been investigated as a store house of bioactive compound. This study was aimed to evaluate the potential antibacterial activity of endophytic fungi isolated from Medinilla speciosa Blume. The antibacterial test was determined by measuring the inhibition zone with disk-diffusion method. Twenty endophytes were isolated from the leaves of Medinilla speciosa Blume and identified morphologically. The results demonstrated that ten isolates showed variation in their antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, and Shigella dysenteriae ATCC 13313. Further investigation will be needed to explore and identify the bioactive molecules of the isolated endophytic fungi.

scholarly journals Characterization of an Endophytic Strain Talaromyces assiutensis, CPEF04 With Evaluation of Production Medium for Extracellular Red Pigments Having Antimicrobial and Anticancer Properties

2021 ◽  
Vol 12 ◽  
Author(s):  
Rahul Chandra Mishra ◽  
Rishu Kalra ◽  
Rahul Dilawari ◽  
Sunil Kumar Deshmukh ◽  
Colin J. Barrow ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Inhibitory Concentration ◽  
Pigment Production ◽  
Array Detector ◽  
Fungal Culture ◽  
Red Pigment ◽  
Hek 293 ◽  
Phylogenetic Characterization ◽  
Marine Habitats ◽  
Cancer Line

Considering the worldwide demand for colorants of natural origin, the utilization of ascomycete fungi as a prolific pigment producer unfolds a novel way to obtain these pigments for various applications, including food, cosmetic, and medical use. The presence of very few natural red pigment alternatives in the market also attracts research and industry priorities to unearth novel and sustainable red pigment producers. The present work is an attempt to identify a novel source of red color obtained from endophytic fungi isolated from terrestrial and marine habitats. Based upon the fungal capacity for pigment production, seven isolates of endophytic fungi were recognized as prospective pigment producers. Out of all, fungal isolate CPE04 was selected based upon its capacity to produce profuse extracellular red pigment. The isolate was identified as Talaromyces assiutensis, employing morphological features and phylogenetic characterization by internal transcribed spacer (ITS) sequences. To understand the chemical behavior of pigment molecules, an investigation of the chemical profile of fungal culture filtrate dried powder (CFDP) was performed using ultra-high-performance liquid chromatography-diode array detector-mass spectrometry (UPLC–DAD–MS). In total, eight compounds having pigment and pharmaceutical application were tentatively identified using UPLC–DAD–MS. Considering the commercial aspect of the stated work, an effort was also made for standardizing the upscaling of the pigment molecule. Investigations were performed for optimum medium and culturing conditions for maximum pigment production. CFDP was found to have a significant antibacterial activity against the bacterial pathogens Staphylococcus aureus (MTCC737), Vibrio cholerae (N16961), and methicillin-resistant S. aureus (MRSA) (ATCC BAA811). The CFDP showed a minimum inhibitory concentration at 64, 128, and 256 μg/ml against S. aureus, MRSA, and V. cholerae. A concentration-dependent (50–400 μg/ml) anticancer effect on HeLa cancer line was also observed, having a half-maximal inhibitory concentration (IC50) at 300 μg/ml. The antioxidant potential of CFDP has also been proven with the help of an antioxidant assay against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (IC50, 32.01 μg/ml); DNA nicking assay and reactive oxygen species were generated in HeLa cancer line cells. The CFDP was also found to have no cytotoxicity toward HEK 293 T cell line using alamar blue (resazurin), a cell metabolic activity reagent.

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