Multidetectors for Analysis of the Composition of Copolymers as a Function of Molecular Size Distribution by Steric Exclusion Chromatography

1973 ◽  
Vol 46 (2) ◽  
pp. 449-463 ◽  
Author(s):  
D. J. Harmon ◽  
V. L. Folt
Keyword(s):  
Liquid Chromatography ◽  
Size Distribution ◽  
Oil Content ◽  
Molecular Size ◽  
The Other ◽  
Additional Information ◽  
Steric Exclusion ◽  
Gel Permeation ◽  
Exclusion Chromatography ◽  
Molecular Size Distribution

Abstract Analysis of molecular size distribution of polymers by steric exclusion liquid chromatography (GPC) is well known. Problems exist, however. These problems involve copolymers and polymer blends. The objectives of the research were to develop methods of analyzing comonomer distribution in copolymers, to study the breakdown of one polymer independent of another in a polymer blend, and to obtain any additional information as might be available. The separations were performed on a Waters Model 200 Gel Permeation Chromatograph. Detectors employed were a Waters R-4 differential refractometer, a Wilks Miran-1 infrared analyzer, and a Beckman Model 144 UV photometer. Examples are given of analysis of average styrene, styrene distribution, and oil content of oil extended SBR. The data is compared with that obtained by other methods. In general the agreement is good. The ability to examine one polymer of a blend independent of the other is also demonstrated. Since elastomers are frequently used as blends, this becomes very important to such studies as milling and extrusion behavior.

Determination of the molecular size distribution of immunoglobulin G (IgG) in intravenous IgG-albumin formulations by high-performance liquid chromatography

1988 ◽  
Vol 444 ◽  
pp. 141-152 ◽  
Author(s):  
John K. Lee ◽  
Frank J. Deluccia ◽  
Ernest L. Kelly ◽  
Catherine Davidson ◽  
Fred R. Borger
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Size Distribution ◽  
Immunoglobulin G ◽  
High Performance ◽  
Molecular Size ◽  
Molecular Size Distribution

scholarly journals A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Imran Khan ◽  
K. M. Taufiqur Rahman ◽  
S. M. Saad Us Siraj ◽  
Mahbubul Karim ◽  
Abdul Muktadir ◽  
...  
Keyword(s):  
Distribution Coefficient ◽  
Size Distribution ◽  
Size Exclusion Chromatography ◽  
Molecular Size ◽  
Polysaccharide Vaccine ◽  
Size Exclusion ◽  
Chromatography Method ◽  
Exclusion Chromatography ◽  
Calculated Distribution ◽  
Molecular Size Distribution

Molecular size distribution of meningococcal polysaccharide vaccine is a readily identifiable parameter that directly correlates with the immunogenicity. In this paper, we report a size exclusion chromatography method to determine the molecular size distribution and distribution coefficient value of meningococcal polysaccharide serogroups A, C, W, and Y in meningococcal polysaccharide (ACWY) vaccines. The analyses were performed on a XK16/70 column packed with sepharose CL-4B with six different batches of Ingovax® ACWY, a meningococcal polysaccharide vaccine produced by Incepta Vaccine Ltd., Bangladesh. A quantitative rocket immunoelectrophoresis assay was employed to determine the polysaccharide contents of each serogroup. The calculated distribution coefficient values of serogroups A, C, W, and Y were found to be0.26±0.16,0.21±0.11,0.21±0.11, and0.14±0.12, respectively, and met the requirements of British Pharmacopeia. The method was proved to be robust for determining the distribution coefficient values which is an obligatory requirement for vaccine lot release.

Determination of the Size Distribution of Dissolved Organics in Effluents

1973 ◽  
Vol 8 (1) ◽  
pp. 1-15 ◽  
Author(s):  
L.A. Addie ◽  
K.L. Murphy ◽  
J.L. Robertson
Keyword(s):  
Size Distribution ◽  
Biological Treatment ◽  
Oxygen Demand ◽  
Molecular Size ◽  
Monitoring Systems ◽  
Clean Surface ◽  
Sephadex Column ◽  
Dissolved Organics ◽  
Molecular Size Distribution

Abstract The importance of removing the small amounts of residual organics is increasing as the sources of clean surface water decrease. Knowledge of the nature of these soluble residual organics will be needed in order to assess the type of treatment required for their removal. Residual organics in three different biological treatment plants were analyzed and compared. An attempt was made to characterize these organics by a molecular size distribution on a Sephadex column monitored by differential ultraviolet and refractive index detectors. The organic carbon and chemical oxygen demand of the fractions collected from the column was also determined. An investigation of some of the problems inherent in the monitoring systems was conducted.

Molecular Size Distribution of Lignin in Wood

Nature ◽  
1967 ◽  
Vol 214 (5086) ◽  
pp. 410-411 ◽  
Author(s):  
W. BROWN ◽  
S. I. FALKEHAG ◽  
E. B. COWLING
Keyword(s):  
Size Distribution ◽  
Molecular Size ◽  
Molecular Size Distribution

A Study of the Malpighian Tubules of the Pill Millipede, Glomeris marginata (Villers)

1974 ◽  
Vol 60 (1) ◽  
pp. 41-51
Author(s):  
PATRICIA ANNE FARQUHARSON
Keyword(s):  
Size Distribution ◽  
Fluid Transport ◽  
Molecular Size ◽  
Malpighian Tubules ◽  
Inverse Relation ◽  
Fluid Production ◽  
Molecular Size Distribution ◽  
Molecular Sieving ◽  
Tubule Fluid ◽  
Tubule Wall

1. Tubule fluid:medium ratios (TF/M) have been measured for inulin, glucose, LMWD and HMWD. These TF/M ratios were surprisingly high. 2. The tubule appears to act as a molecular filter; that is to say, molecules move through the tubule wall in inverse relation to their size. This is best illustrated using polyvinyl pyrrolidone as a tracer. The molecular size distribution of PVP fractions present in tubule fluid differs markedly from the molecular size distribution of PVP in the bathing Ringer. 3. No correlation can be made between the inulin and glucose TF/M and the rate of fluid production. However, the inverse relationship between TF/M and rate of fluid production for dextrans indicates a molecular sieving effect. 4. The significance of these results is discussed with reference to models of fluid transport.

Sign in / Sign up

Export Citation Format

Share Document