The Detection of Biomarkers

The potential of first-void (FV) urine as a non-invasive liquid biopsy for detection of human papillomavirus (HPV) DNA and other biomarkers has been increasingly recognized over the past decade. In this study, we investigated whether the volume of this initial urine stream has an impact on the analytical performance of biomarkers. In parallel, we evaluated different DNA extraction protocols and introduced an internal control in the urine preservative. Twenty-five women, diagnosed with high-risk HPV, provided three home-collected FV urine samples using three FV urine collection devices (Colli-Pee) with collector tubes that differ in volume (4, 10, 20 mL). Each collector tube was prefilled with Urine Conservation Medium spiked with phocine herpesvirus 1 (PhHV-1) DNA as internal control. Five different DNA extraction protocols were compared, followed by PCR for GAPDH and PhHV-1 (qPCR), HPV DNA, and HBB (HPV-Risk Assay), and ACTB (methylation-specific qPCR). Results showed limited effects of collection volume on human and HPV DNA endpoints. In contrast, significant variations in yield for human endpoints were observed for different DNA extraction methods (p < 0.05). Additionally, the potential of PhHV-1 as internal control to monitor FV urine collection, storage, and processing was demonstrated.

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Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices

Scientific Reports ◽

10.1038/s41598-021-88546-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Carlotta Peruzzi ◽

Silvia Battistoni ◽

Daniela Montesarchio ◽

Matteo Cocuzza ◽

Simone Luigi Marasso ◽

...

Keyword(s):

Biomedical Applications ◽

Au Nanoparticles ◽

Limit Of Detection ◽

High Sensitivity ◽

Minimal Invasiveness ◽

Hierarchic Structure ◽

Final Response ◽

Detection Of Biomolecules ◽

Good Signal ◽

Detection Of Biomarkers

AbstractIn several biomedical applications, the detection of biomarkers demands high sensitivity, selectivity and easy-to-use devices. Organic electrochemical transistors (OECTs) represent a promising class of devices combining a minimal invasiveness and good signal transduction. However, OECTs lack of intrinsic selectivity that should be implemented by specific approaches to make them well suitable for biomedical applications. Here, we report on a biosensor in which selectivity and a high sensitivity are achieved by interfacing, in an OECT architecture, a novel gate electrode based on aptamers, Au nanoparticles and graphene hierarchically organized to optimize the final response. The fabricated biosensor performs state of the art limit of detection monitoring biomolecules, such as thrombin-with a limit of detection in the picomolar range (≤ 5 pM) and a very good selectivity even in presence of supraphysiological concentrations of Bovine Serum Albumin (BSA-1mM). These accomplishments are the final result of the gate hierarchic structure that reduces sterich indrance that could contrast the recognition events and minimizes false positive, because of the low affinity of graphene towards the physiological environment. Since our approach can be easily applied to a large variety of different biomarkers, we envisage a relevant potential for a large series of different biomedical applications.

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Aptamer-linked in vitro expression assay for ultrasensitive detection of biomarkers

Analytica Chimica Acta ◽

10.1016/j.aca.2020.12.036 ◽

2021 ◽

Vol 1146 ◽

pp. 118-123

Author(s):

Min-Seok Baek ◽

Kyung-Ho Lee ◽

Ju-Young Byun ◽

Yong-Beom Shin ◽

Dong-Myung Kim

Keyword(s):

Ultrasensitive Detection ◽

In Vitro Expression ◽

Detection Of Biomarkers

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Metal Nanoparticle and Quantum Dot Tags for Signal Amplification in Electrochemical Immunosensors for Biomarker Detection

Chemosensors ◽

10.3390/chemosensors9040085 ◽

2021 ◽

Vol 9 (4) ◽

pp. 85

Author(s):

Anton Popov ◽

Benediktas Brasiunas ◽

Asta Kausaite-Minkstimiene ◽

Almira Ramanaviciene

Keyword(s):

Signal Amplification ◽

Electrochemical Immunosensor ◽

Metal Nanoparticle ◽

Biomarker Detection ◽

Analytical Instruments ◽

Electrochemical Immunosensors ◽

Highly Sensitive ◽

Recent Developments ◽

Electrochemical Signal ◽

Detection Of Biomarkers

With the increasing importance of healthcare and clinical diagnosis, as well as the growing demand for highly sensitive analytical instruments, immunosensors have received considerable attention. In this review, electrochemical immunosensor signal amplification strategies using metal nanoparticles (MNPs) and quantum dots (Qdots) as tags are overviewed, focusing on recent developments in the ultrasensitive detection of biomarkers. MNPs and Qdots can be used separately or in combination with other nanostructures, while performing the function of nanocarriers, electroactive labels, or catalysts. Thus, different functions of MNPs and Qdots as well as recent advances in electrochemical signal amplification are discussed. Additionally, the methods most often used for antibody immobilization on nanoparticles, immunoassay formats, and electrochemical methods for indirect biomarker detection are overviewed.

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P3.364 Does Tenofovir Gel - or Do Other Vaginal Microbicide Products - Affect Detection of Biomarkers of Semen Exposure?

Sexually Transmitted Infections ◽

10.1136/sextrans-2013-051184.0817 ◽

2013 ◽

Vol 89 (Suppl 1) ◽

pp. A263.1-A263

Author(s):

M C Snead ◽

A P Kourtis ◽

J H Melendez ◽

C M Black ◽

C K Mauck ◽

...

Keyword(s):

Vaginal Microbicide ◽

Affect Detection ◽

Tenofovir Gel ◽

Detection Of Biomarkers

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Gold nanoparticles in the clinical laboratory: principles of preparation and applications

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2011.732 ◽

2012 ◽

Vol 50 (2) ◽

Cited By ~ 34

Author(s):

Hassan M.E. Azzazy ◽

Mai M.H. Mansour ◽

Tamer M. Samir ◽

Ricardo Franco

Keyword(s):

Gold Nanoparticles ◽

Clinical Laboratory ◽

Sensitive Detection ◽

Detection Methods ◽

Practical Applications ◽

Molecular Assays ◽

Detection Of Biomarkers

AbstractIn order to meet the challenges of effective healthcare, the clinical laboratory is constantly striving to improve testing sensitivity while reducing the required time and cost. Gold nanoparticles (AuNPs) are proposed as one of the most promising tools to meet such goals. They have unique optophysical properties which enable sensitive detection of biomarkers, and are easily amenable to modification for use in different assay formats including immunoassays and molecular assays. Additionally, their preparation is relatively simple and their detection methods are quite versatile. AuNPs are showing substantial promise for effective practical applications and commercial utilization is already underway. This article covers the principles of preparation of AuNPs and their use for development of different diagnostic platforms.

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Liquid crystal-based sensors for the detection of biomarkers at the aqueous/LC interface

TrAC Trends in Analytical Chemistry ◽

10.1016/j.trac.2021.116434 ◽

2021 ◽

pp. 116434

Author(s):

Mashooq Khan ◽

Shuya Liu ◽

Lubin Qi ◽

Chunxia Ma ◽

Sundas Munir ◽

...

Keyword(s):

Liquid Crystal ◽

Detection Of Biomarkers

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Application progress of magnetic molecularly imprinted polymer chemical sensors in the detection of biomarkers

The Analyst ◽

10.1039/d1an01112j ◽

2022 ◽

Author(s):

Ying Wang ◽

Xiaomin Yang ◽

Lin Pang ◽

Pengfei Geng ◽

Fang Mi ◽

...

Keyword(s):

Chemical Sensors ◽

Molecularly Imprinted Polymers ◽

Essential Role ◽

Imprinted Polymer ◽

Molecularly Imprinted ◽

Magnetic Molecularly Imprinted Polymer ◽

Magnetic Molecularly Imprinted Polymers ◽

Highly Sensitive ◽

Detection Of Biomarkers ◽

Highly Sensitive Detection

Specific recognition and highly sensitive detection of biomarkers play an essential role in identifying, early diagnosis and prevention of many diseases. Magnetic molecularly imprinted polymers (MMIP) have been widely used...

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