Analysis of perchlorate, thiocyanate, nitrate and iodide in human amniotic fluid using ion chromatography and electrospray tandem mass spectrometry

2006 ◽  
Vol 567 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Benjamin C. Blount ◽  
Liza Valentin-Blasini
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Amniotic Fluid ◽  
Ion Chromatography ◽  
Tandem Mass ◽  
Electrospray Tandem Mass Spectrometry ◽  
Human Amniotic Fluid

Analysis of Perchlorate in Human Urine Using Ion Chromatography and Electrospray Tandem Mass Spectrometry

2005 ◽  
Vol 77 (8) ◽  
pp. 2475-2481 ◽  
Author(s):  
Liza Valentín-Blasini ◽  
Joshua P. Mauldin ◽  
David Maple ◽  
Benjamin C. Blount
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Ion Chromatography ◽  
Human Urine ◽  
Tandem Mass ◽  
Electrospray Tandem Mass Spectrometry

scholarly journals Quantifying steroid hormones in amniotic fluid by ultra-performance liquid chromatography and tandem mass spectrometry

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1736 ◽  
Author(s):  
Noëllie Rivet ◽  
Carole Jamey ◽  
Nathalie Reix ◽  
Pascal Kintz ◽  
Martin Heil ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Amniotic Fluid ◽  
Steroid Hormone ◽  
Ultra Performance Liquid Chromatography ◽  
Male Offspring ◽  
Tandem Mass ◽  
Human Amniotic Fluid ◽  
Assay Precision

Background: Simultaneous assessment of steroid hormone concentrations in amniotic fluid is of importance for elucidating long-term consequences of intrauterine processes, and of broad scientific and clinical relevance. The objective of the study was to develop sensitive and specific analytical ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) methods for determination of steroid hormone concentrations in human amniotic fluid, and to provide proof-of-concept of their applicability. Methods: Methods were validated according to linearity, limit-of-detection, limit-of-quantification, recovery, intra- and inter-assay precision, and applied to 275 amniotic fluid samples. Results: Limits-of-quantification (S/N=10:1) were 0.05 ng/mL for cortisol, dehydroepiandrosterone sulfate (DHEAS), estradiol, estriol, and testosterone, and 0.01 ng/mL and 1.0 ng/mL for cortisone and dehydroepiandrosterone (DHEA), respectively. Good inter- and intra-assay precision were observed. Cortisol, cortisone, DHEAS, estradiol and estriol concentration were quantified in all samples. By lack of sensitivity of the analytical method, DHEA was quantified in 11 samples only. Testosterone was quantified in 119 of 275 samples analyzed. 116 of the quantified testosterone samples were from male offspring (out of 138 male offspring). Conclusion: These specific and sensitive methods offer a simple and non-invasive way to measure cortisol, cortisone, DHEAS, estradiol, estriol, and testosterone concentrations in human amniotic fluid.

Determination of Trace Level Perchlorate in Sludge Samples by Ion Chromatography-Electrospray Tandem Mass Spectrometry

2007 ◽  
Vol 35 (1) ◽  
pp. 131-134 ◽  
Author(s):  
Ping Zhang ◽  
Ya-Li Shi ◽  
Ya-Wei Wang ◽  
Ya-Qi Cai ◽  
Shi-Fen Mou
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Ion Chromatography ◽  
Tandem Mass ◽  
Electrospray Tandem Mass Spectrometry ◽  
Trace Level

Analysis of Brassinosteroids in Soybean Seeds and Leaves by Liquid Chromatography-Tandem Mass Spectrometry

2017 ◽  
Vol 10 (1) ◽  
pp. 100-109
Author(s):  
Hongxia Li ◽  
Natasha Trzaskalski ◽  
R.J. Neil Emery
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Soybean Seeds ◽  
Tandem Mass ◽  
Electrospray Tandem Mass Spectrometry ◽  
Aqueous Methanol ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Objective:A simple and fast high performance liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) method has been developed for the analysis of brassinosteroids (BRs) in plants without derivatization.Materials:The BRs (including castasterone, 24-epicastasterone, brassinolide and 24-epibrassinolide) have been extracted with ice cold 80% aqueous methanol solution.Method:Five different purification strategies have been tested for the purification and enrichment of BRs.Conclusion:This analytical method was sensitive, reliable, rapid and applicable to trace analysis in complex plant samples.

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