Antimicrobial resistance of 3 types of gram-negative bacteria isolated from hospital surfaces and the hands of health care workers

2017 ◽  
Vol 45 (11) ◽  
pp. e143-e147 ◽  
Author(s):  
Hui-Ping Wang ◽  
Hong-Jiang Zhang ◽  
Jie Liu ◽  
Qi Dong ◽  
Shan Duan ◽  
...  
Keyword(s):  
Health Care ◽  
Antimicrobial Resistance ◽  
Health Care Workers ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Care Workers

Awareness of Antimicrobial Resistance among Primary Health Care Workers in Buyende District, Rural Eastern Uganda

2018 ◽  
Vol 22 (5) ◽  
pp. 1-11 ◽  
Author(s):  
Ahebwa Amelia ◽  
Akol Walter ◽  
Achong Emmanuel ◽  
Mugerwa Timothy ◽  
Nakayenga Aminah ◽  
...  
Keyword(s):  
Health Care ◽  
Primary Health Care ◽  
Antimicrobial Resistance ◽  
Health Care Workers ◽  
Primary Health ◽  
Care Workers ◽  
Eastern Uganda

Indications for Alcohol or Bland Soap in Removal of Aerobic Gram-Negative Skin Bacteria: Assessment by a Novel Method

1989 ◽  
Vol 10 (7) ◽  
pp. 306-311 ◽  
Author(s):  
Debra G. Eckert ◽  
N. Joel Ehrenkranz ◽  
Blanca C. Alfonso
Keyword(s):  
Health Care ◽  
Health Care Workers ◽  
Complete Removal ◽  
Contact Model ◽  
Patient Contact ◽  
Gram Negative ◽  
High Burden ◽  
Skin Bacteria ◽  
Care Workers ◽  
Novel Method

AbstractA patient contact model was devised for health care workers (HCWs) to define heavy hand contamination with aerobic gram-negative bacilli (AGNB) that requires alcohol for complete removal. In patients, AGNB quantitation was per ml cup scrub fluid; in HCWs, per ml glove juice. Following 15-second contact, two Proteeae groin carriers yielding ≥ 4 log10AGNB (high burden) transmitted ≥ 3 log10in 67% of 24 tests of six HCWs, and ≤ 2 log10in 29%. Two carriers yielding ≤ 3 log10(low burden) transmitted ≥ 3 log10in 8% and ≤ 2 log10in 38%. At ≤ 2 log10HCW acquisitions, soap eliminated all AGNB in six of seven tests; alcohol in nine of nine (p > 0.05). At ≥ 3 log10acquisitions, soap eliminated all AGNB in three of 10; alcohol in eight of eight Contacteight (p= 0.009). Contact with densely colonized patient skin may cause heavy AGNB contamination of HCWs' hands that generally necessitates alcohol for complete removal.

scholarly journals Pathogenic microbes contaminating mobile phones in hospital environment in Northeast India: incidence and antibiotic resistance

2019 ◽  
Vol 47 (1) ◽  
Author(s):  
Christine Vanlalbiakdiki Sailo ◽  
Puja Pandey ◽  
Subhajit Mukherjee ◽  
Zothan Zami ◽  
Ralte Lalremruata ◽  
...  
Keyword(s):  
Health Care ◽  
Mobile Phones ◽  
Health Care Workers ◽  
Drug Susceptibility ◽  
Cefuroxime Axetil ◽  
Hospital Environment ◽  
Gram Positive ◽  
Gram Negative ◽  
Care Workers ◽  
Gram Negative Organisms

Abstract Background The present study attempts to identify and determine the pattern of drug susceptibility of the microorganisms present in mobile phones of health care workers (HCWs) and non-HCWs in a hospital environment. Mobile phones of 100 participants including both genders were randomly swabbed from nine different wards/units and the bacterial cultures were characterized using VITEK 2 system. Results Forty-seven mobile phones were culture positive and a total of 57 isolates were obtained which consisted of 28 Gram-positive organisms and 29 Gram-negative organisms. The predominating organisms were Acinetobacter baumannii and Staphylococcus hominis. Among all the isolates from the mobile phones of HCW and non-HCWs, five isolates had ESBL and three isolates had colistin resistance. Incidentally, MRSA was not found on the mobile phones tested. The isolated organisms showed 100% susceptibility to linezolid, daptomycin, vancomycin, imipenem, meropenem, gentamicin, amikacin, ciprofloxacin and tigecycline, while high resistance was shown against benzylpenicillin (75.0%), cefuroxime and cefuroxime axetil (56.5%). Non-HCWs’ mobile phones were more contaminated as compared to HCWs (P = 0.001) and irrespective of individuals’ gender or toilet habits, both Gram-positive and Gram-negative organisms were present on the mobile phones. Conclusion This study reports for the first time that the mobile phones of non-health care workers harbour more bacterial diversity and are more prone to cause transmission of pathogens. This study can serve to educate the public on personal hand hygiene practices and on maintaining clean mobile phones through antiseptic measures.

scholarly journals Hand carriage of aerobic Gram-negative rods by health care personnel

1982 ◽  
Vol 89 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Brian G. Adams ◽  
Thomas J. Marrie
Keyword(s):  
Health Care ◽  
Health Care Workers ◽  
Acinetobacter Calcoaceticus ◽  
Culture Technique ◽  
Health Care Personnel ◽  
Enterobacter Agglomerans ◽  
Gram Negative ◽  
Control Subjects ◽  
Care Workers ◽  
Normal Hand

SUMMARYA quantitative culture technique (hand washed in a glove containing broth for 30 s) was used to determine the frequency of hand carriage of aerobic Gram-negative rods by various groups of health care workers and 104 control subjects. Overall, 31 % of health care workers carried aerobic Gram-negative rods on their hands compared to 59% of control subjects (P< 0.001).Enterobacter agglomeransaccounted for 40% of the isolates, and otherEnterobacterspp. 7%. Other organisms includedAcinetobacter calcoaceticus21%, Serratiaspp. 11%,Klebsiellaspp. 10%,Moraxellaspp. 3%,Pseudomonasspp. 3%,Proteusspp. 1.5%,Escherichia coli1%;Morganella morganii, Citrobacter freundii, Aeromonassp. and an isolate that was not speciated accounted for 0.5% each. We conclude that endemic hand carriage of aerobic Gram-negative rods by health care personnel is common, but significantly less than that of control subjects.Enterobacter agglomeransis found so frequently on the hands of control subjects that it must be considered part of the normal hand flora.

scholarly journals Hand carriage of aerobic Gram-negative rods may not be transient

1982 ◽  
Vol 89 (1) ◽  
pp. 33-46 ◽  
Author(s):  
Brian G. Adams ◽  
Thomas J. Marrie
Keyword(s):  
Health Care ◽  
Health Care Workers ◽  
Surgical Intensive Care Unit ◽  
Control Group ◽  
Enterobacter Agglomerans ◽  
Surgical Intensive Care ◽  
Gram Negative ◽  
Hand Dermatitis ◽  
Control Subjects ◽  
Care Workers

SUMMARYIn order to determine whether hand carriage of aerobic Gram-negative rods is continuous we used the glove-handwash technique to sample the hands of two groups (four each) of health care workers with normal hands (surgical intensive care unit, medical ward) and one group (four) with hand dermatitis (HD) and a group (five) of control subjects – secretaries with no exposure to patients. Each subject was sampled repeatedly over three to six weeks. The mean number of samples for each group was 25.2, 23.2, 19.8 and 25.8 respectively. The HD group had more samples positive for aerobic Gram-negative rods than did the other two groups of health care workers while the control group had more samples positive than any of the three health care groups.Using various typing schemes and the following definition of continuous carriage (the isolation of an organism of the same serotype, pyocin type or biotype from more than two handwash samples) we found that 4 of 11 subjects from whomKlebsiella pseumoniaewas isolated carried this organism continuously; 2 of 3 carriedPseudomonas aeruginosacontinuously and 4 of 5 of the control subjects carried the same biotype ofEnterobacter agglomeranscontinuously.We conclude that continuous hand carriage of aerobic Gram-negative rods is common and, among health care workers, those with hand dermatitis carry Gram-negative rods more frequently and in greater numbers than health care workers without hand dermatitis.

scholarly journals Gram Positive Bacteria Carriage among Health Care Workers: An Under-Reported Source of Infections?

2020 ◽  
Vol 14 (4) ◽  
pp. 2677-2682
Author(s):  
Vaishnavi Toshniwal ◽  
Gargi Mudey ◽  
Aditya Khandekar ◽  
Vandana Kubde ◽  
Abhay Mudey
Keyword(s):  
Health Care ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Streptococcus Pyogenes ◽  
Health Care Workers ◽  
Blood Agar ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Care Workers ◽  
Oropharyngeal Carriage

Staphylococcus aureus and Streptococcus pyogenes are two highly infectious pathogens implicated in a significant percentage of healthcare associated infections. They produce wide range of infections, from mere folliculitis & furuncles, cellulitis, myositis, & glomerulonephritis to conditions with very significant morbidity such as necrotizing fasciitis & Toxic Shock syndrome, and thus represent an important subset of infections that need to be tackled urgently. To assess prevalence of nasal as well as oropharyngeal carriage of Staphylococcus aureus & Streptococcus pyogenes among health-care workers and its antimicrobial resistance pattern. One nasal swab & two oropharyngeal swabs were collected from each participant, with one nasal & oropharyngeal swab cultured on blood agar & mannitol salt agar for Staphylococcus aureus, and the second oropharyngeal swabs were cultured on Crystal violet blood agar for Streptococcus pyogenes, further subjected to susceptibility test by disc diffusion method on Muller-Hinton agar as per CLSI guidelines 2019. Prevalence of Staphylococcus aureus carriage was 9% which includes 4% It is nasal, 4.5% oropharyngeal & 0.5% both. Prevalence of MRSA, MLSB & mupirocin resistant Staphylococcus aureus was 1.5%,4% & 0%respectively. Prevalence of oropharyngeal carriage of Streptococcus pyogenes was 1.5%. This study feature the need of screening of Health-care workers for nasal as well as oropharyngeal carriage of Staphylococcus aureus & Streptococcus pyogenes & further its antimicrobial resistance pattern.

Organizational Factors and Health Care Workers' Physical Safety

2013 ◽  
Author(s):  
Jane Lipscomb ◽  
Jeanne Geiger-Brown ◽  
Katherine McPhaul ◽  
Karen Calabro
Keyword(s):  
Health Care ◽  
Health Care Workers ◽  
Organizational Factors ◽  
Care Workers
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