scholarly journals Gram Positive Bacteria Carriage among Health Care Workers: An Under-Reported Source of Infections?

2020 ◽  
Vol 14 (4) ◽  
pp. 2677-2682
Author(s):  
Vaishnavi Toshniwal ◽  
Gargi Mudey ◽  
Aditya Khandekar ◽  
Vandana Kubde ◽  
Abhay Mudey
Keyword(s):  
Health Care ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Streptococcus Pyogenes ◽  
Health Care Workers ◽  
Blood Agar ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Care Workers ◽  
Oropharyngeal Carriage

Staphylococcus aureus and Streptococcus pyogenes are two highly infectious pathogens implicated in a significant percentage of healthcare associated infections. They produce wide range of infections, from mere folliculitis & furuncles, cellulitis, myositis, & glomerulonephritis to conditions with very significant morbidity such as necrotizing fasciitis & Toxic Shock syndrome, and thus represent an important subset of infections that need to be tackled urgently. To assess prevalence of nasal as well as oropharyngeal carriage of Staphylococcus aureus & Streptococcus pyogenes among health-care workers and its antimicrobial resistance pattern. One nasal swab & two oropharyngeal swabs were collected from each participant, with one nasal & oropharyngeal swab cultured on blood agar & mannitol salt agar for Staphylococcus aureus, and the second oropharyngeal swabs were cultured on Crystal violet blood agar for Streptococcus pyogenes, further subjected to susceptibility test by disc diffusion method on Muller-Hinton agar as per CLSI guidelines 2019. Prevalence of Staphylococcus aureus carriage was 9% which includes 4% It is nasal, 4.5% oropharyngeal & 0.5% both. Prevalence of MRSA, MLSB & mupirocin resistant Staphylococcus aureus was 1.5%,4% & 0%respectively. Prevalence of oropharyngeal carriage of Streptococcus pyogenes was 1.5%. This study feature the need of screening of Health-care workers for nasal as well as oropharyngeal carriage of Staphylococcus aureus & Streptococcus pyogenes & further its antimicrobial resistance pattern.

scholarly journals Study to Assess the Risk of Transmission of Microbial Organisms and their Resistance Pattern on Dresses and Stethoscopes of Health Care Workers

2021 ◽  
Author(s):  
E. Nivedhitha ◽  
M. Duraivel ◽  
K.K. Kayalvili ◽  
S. Arul Selvan
Keyword(s):  
Health Care ◽  
Staphylococcus Aureus ◽  
Health Care Workers ◽  
Hospital Environment ◽  
Penicillin G ◽  
Resistance Pattern ◽  
Coagulase Negative Staphylococci ◽  
Klebsiella Species ◽  
Care Workers ◽  
Microbial Organisms

The main objective of the study was to find out the contamination of different types of microbial organisms and their resistance pattern on the dresses and stethoscopes of the health care workers in order to evaluate the risk of transmission of microbial organisms and its consequences on safety of the patient and control of hospital acquired infections (HAIs). Swabs were taken from the dresses of 100 different healthcare workers from 4 different areas namely collar, pocket, sleeves and sides and 100 stethoscopes (diaphragm) were tested for bacteriological analysis using standard techniques. Then each health care worker were given a structured questionnaire and requested to fill the form which includes his or her speciality/unit, cadre, practice of hand hygiene, white-coat or uniform usage (Example: duration of usage, frequency of washing, type of washing etc). The collar and pockets were found to be the most contaminated areas. Gram-positive cocci such as Staphylococcus aureus and Coagulase-negative Staphylococci (CONS) were the important organisms isolated from the dresses and stethoscopes of health care workers followed by Gram-negative bacilli such as Pseudomonas aeruginosa and Klebsiella species. Among the microbial organisms, Staphylococcus aureus and CONS were resistant to the drugs like Co-trimoxazole and Penicillin-G. Organisms isolated from the dresses and stethoscopes of health care workers could be a source of infection to immunocompromised patients. Hence, frequent washing of the dresses and strict disinfectant practices of the stethoscopes will minimize the contamination with microbial organisms and the patient safety is improved in the hospital environment.

scholarly journals Nasal and Hands Carriage Rate of Methicillin-Resistant Staphylococcus aure-us among Health Care Workers at Alwahda Hospital, Derna

2019 ◽  
Vol 34 (4) ◽  
pp. 279-287
Author(s):  
Hussain Y. H. Khnfar ◽  
Dalal A. Thaood ◽  
Ilham O. A. Abdraba ◽  
Intesar N. Omran ◽  
Guma M. K. Abdeldaim
Keyword(s):  
Health Care ◽  
Staphylococcus Aureus ◽  
Infection Control ◽  
Health Care Workers ◽  
Healthcare Workers ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Diffusion Method ◽  
Mannitol Salt Agar ◽  
Methicillin Resistant ◽  
Care Workers

Increasing incidence of Methicillin-Resistant Staphylococcus aureus (MRSA) is a well-documented healthcare and community phenomenon of concern to medical and public health communities all over the world. One of the most important sources of MRSA infection in hospitals is health care workers (HCWs) through nasal or hands carriage. The aim of this study is to determine the rate of methicillin-resistant Staphylococcus aureus among HCWs. A cross-sectional study involving 102 HCWs was conducted at Alwahda hospital, Derna. Nasal and hand swabs were collected and cultured on Mannitol Salt Agar. Gram staining was performed on mannitol fermenting colonies. Slide catalase and coagulase were performed to identify S. aureus. An oxacillin and cefoxitin susceptibility test was carried out on Muller Hinton agar using the modified Kirby-Bauer disc diffusion method. Results: out of 102 healthcare workers, 46 (45.1%) carried S. aureus. The rate of methicillin resistance amongst all S. aureus isolates was 47.8 % (22/46) whereas 21.6 % (22/102) of all HCWs were identified as MRSA carriers. Nurses had the highest MRSA carriage with 53%, compared to other HCW. On the other hand, the highest rate of MRSA (75%) was seen in CCU workers followed by workers of pediatric and surgical wards (66%, 62% respectively). The high rate of nasal and hand MRSA carriage among healthcare workers, especially in high-risk wards, indicates the imperfection of infection control measures in our hospitals and the necessity to improve the infection control program.

scholarly journals Mupirocin Resistance in Staphylococcus aureus Isolated from Nasal Swabs of ICU and OT Staff- A Study from A Tertiary Care Hospital

2021 ◽  
Author(s):  
Sapna Chauhan ◽  
Surender ◽  
Tony J Rappai
Keyword(s):  
Health Care ◽  
Staphylococcus Aureus ◽  
Health Care Workers ◽  
Tertiary Care ◽  
Nasal Carriage ◽  
Diffusion Method ◽  
Care Hospital ◽  
Care Workers ◽  
Nasal Swabs ◽  
Mupirocin Resistance

Staphylococcus aureus is one of the common causes of Healthcare-associated infection. Staphylococcus colonizes the anterior nares of the nose and tends to disseminate and secondarily colonize several other body sites including the skin and the gut. Colonized hospital personnel may be an important factor in dissemination. Staphylococcus aureus to patients and vice-versa. Mupirocin is an excellent topical anti-staphylococcal antimicrobial agent used for eradicating nasal carriage. Resistance to Mupirocin is a threat for future use of this drug in eliminating nasal carriage of Staphylococcus aureus. Thus, this study was conducted to determine the rate of Mupirocin resistance among Staphylococcus aureus isolated from nasal swabs of Health care workers (HCWs ) of Operation Theatres (OTs) and Intensive Care Units (ICUs). A single nasal swab was collected from both the anterior nares of participating health care workers of ICU and OT once at the end of their shift. Antibiotic susceptibility testing of Staphylococcus aureus to various antibiotics was done by Kirby-Bauer disk diffusion method using CLSI guidelines. High and low-level Mupirocin resistance was determined. Among 282 nasal swabs collected, Staphylococcus aureus was isolated in 62 samples. Of Staphylococcus aureus 19 came out to be Methicillin-resistant (MRSA) and the remaining 43 Methicillin sensitive (MSSA). Mupirocin resistance was seen in 3 MRSA strains and 1 MSSA strain. Thus, overall 4/62 (6.5%) strains were MupR strains. Mupirocin is the most effective antibiotic used against colonization of Staphylococcus aureus in anterior nares. Resistance to this antibiotic is thus an alarm as well as a matter of great concern. Necessary steps, policies and guidelines need to be framed to stop the spread of this resistance.

scholarly journals Prevalence and risk factors for Staphylococcus aureus in health care workers at a University Hospital of Recife-PE

2008 ◽  
Vol 12 (6) ◽  
pp. 504-508 ◽  
Author(s):  
Eduardo Caetano Brandão Ferreira da Silva ◽  
Maria das Graças C. Antas ◽  
Armando Monteiro B. Neto ◽  
Marcelle Aquino Rabelo ◽  
Fábio Lopes de Melo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Health Care ◽  
Staphylococcus Aureus ◽  
Health Care Workers ◽  
University Hospital ◽  
Care Workers

scholarly journals To determine the resistance pattern of Staphylococcus aureus in pus samples

2021 ◽  
Vol 7 (4) ◽  
pp. 292-294
Author(s):  
Vasundhara Sharma ◽  
Versha Rajput ◽  
Umar Farooq ◽  
Sudhir Singh ◽  
Shweta R Sharma ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Sensitivity ◽  
Positive Culture ◽  
Blood Stream ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Staph Aureus ◽  
Care Workers ◽  
Accurate Treatment ◽  
Patient’S Outcome

Staphylococcus aureus is a common health problem occuring as an important nosocomial pathogen, causing urinary tract infection, surgical site, blood stream and soft tissue infection. The aim of this research was conducted to determine MRSA and VRSA from the pus samples of admitted patients.The aim and objective of study was to isolate the resistance pattern of Staphylococcus aureus in pus samples and their AST. A total of 158 positive culture Staph aureus were taken from pus samples for the study during December 2019 - October 2020. Samples were cultured on Blood and MacConkey agar then incubated at 37C for 24 hours. The modified Kirby Bauer's disc diffusion method was used to test antibiotic sensitivity of staphylococcus isolates. In total of 158 positive culture of Staphylococcus aureus, 66 (41.7%) were found to be MRSA and 4 (2.5%) were found to be VRSA. Out of 158 Staph aureus, 146 (92.4%) were resistant to Penicillin, followed by Amoxycillin 140 (88.6%), Ampicillin 139 (87.9%), Erythromycin 91 (57.5%), Cefoxitin 66 (41.7%), Gentamycin 56 (35.4%), Amikacin 52 (32.9%) and Teicoplanin 37 (23.4%).: An antibiotic policy and screening of susceptibility patterns of MRSA may help in reducing the prevalence rate of MRSA and antibiotic resistance. To stop its spread to the population, it is very important to eliminate MRSA colonization in patients and health care workers. Accurate treatment helps to reduce the rate of morbidity and improvement of patient’s outcome.

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