scholarly journals Vitamin D level and endogenous DNA damage in patients with cancers in Duhok city, KRG-Iraq

2020 ◽  
Vol 60 ◽  
pp. 462-467
Author(s):  
Hishyar Azo Najeeb ◽  
Ramadhan Othman ◽  
Sherwan F. Salih ◽  
Ayad Ahmad Mohammed ◽  
Qais Al Ismaeel
Keyword(s):  
Vitamin D ◽  
Dna Damage

scholarly journals Obesity, oxidative DNA damage and vitamin D as predictors of genomic instability in children and adolescents

2021 ◽  
Author(s):  
Moonisah Usman ◽  
Maria Woloshynowych ◽  
Jessica Carrilho Britto ◽  
Ivona Bilkevic ◽  
Bethany Glassar ◽  
...  
Keyword(s):  
Vitamin D ◽  
Dna Damage ◽  
Genomic Instability ◽  
Oxidative Dna Damage ◽  
Nutritional Deficiencies ◽  
Aetiological Factor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Childhood And Adolescence ◽  
Paediatric Obesity ◽  
Vitamin D Levels

Abstract Background/objectives Epidemiological evidence indicates obesity in childhood and adolescence to be an independent risk factor for cancer and premature mortality in adulthood. Pathological implications from excess adiposity may begin early in life. Obesity is concurrent with a state of chronic inflammation, a well-known aetiological factor for DNA damage. In addition, obesity has been associated with micro-nutritional deficiencies. Vitamin D has attracted attention for its anti-inflammatory properties and role in genomic integrity and stability. The aim of this study was to determine a novel approach for predicting genomic instability via the combined assessment of adiposity, DNA damage, systemic inflammation, and vitamin D status. Subjects/methods We carried out a cross-sectional study with 132 participants, aged 10–18, recruited from schools and paediatric obesity clinics in London. Anthropometric assessments included BMI Z-score, waist and hip circumference, and body fat percentage via bioelectrical impedance. Inflammation and vitamin D levels in saliva were assessed by enzyme-linked immunosorbent assay. Oxidative DNA damage was determined via quantification of 8-hydroxy-2′-deoxyguanosine in urine. Exfoliated cells from the oral cavity were scored for genomic instability via the buccal cytome assay. Results As expected, comparisons between participants with obesity and normal range BMI showed significant differences in anthropometric measures (p < 0.001). Significant differences were also observed in some measures of genomic instability (p < 0.001). When examining relationships between variables for all participants, markers of adiposity positively correlated with acquired oxidative DNA damage (p < 0.01) and genomic instability (p < 0.001), and negatively correlated with vitamin D (p < 0.01). Multiple regression analyses identified obesity (p < 0.001), vitamin D (p < 0.001), and oxidative DNA damage (p < 0.05) as the three significant predictors of genomic instability. Conclusions Obesity, oxidative DNA damage, and vitamin D deficiency are significant predictors of genomic instability. Non-invasive biomonitoring and predictive modelling of genomic instability in young patients with obesity may contribute to the prioritisation and severity of clinical intervention measures.

scholarly journals Effects of Hypocalcemic Vitamin D Analogs in the Expression of DNA Damage Induced in Minilungs from Hescs: Implications for Lung Fibrosis

2021 ◽  
Author(s):  
Esmeralda Magro-Lopez ◽  
Irene Chamorro-Herrero ◽  
Alberto Zambrano
Keyword(s):  
Vitamin D ◽  
Dna Damage ◽  
Cell Line ◽  
Lung Fibrosis ◽  
Cell Senescence ◽  
Biologically Active ◽  
Therapeutic Effects ◽  
Lung Pathology ◽  
Alveolar Cells ◽  
Vitamin D Analogs

Abstract BackgroundIn our previous work, we evaluated the therapeutic effects of 1α,25-Dihydroxyvitamin D3, the biologically active form of vitamin D, in the context of bleomycin-induced lung fibrosis. Contrary to the expected, vitamin D supplementation increased DNA damage expression and cellular senescence in alveolar epithelial type II cells and aggravated the overall lung pathology induced in mice by bleomycin. These effects were probably due to an alteration of the cellular DNA double-strand breaks repair capability. In the present work we have evaluated the effects of two hypocalcemic vitamin D analogs (calcipotriol and paricalcitol) in the expression of DNA damage in the context of minilungs derived from human embryonic stem cells and in the cell line A549.ResultsAs in the case of the cell line A549, bleomycin can induce DNA damage in the generated minilungs enriched in alveolar cells. The results indicate that, in contrast to vitamin D, the treatment of the minilungs with the hypocalcemic analogs reduce significantly the bulk of DNA damage expression in both bidimensional arrays of epithelial cells (2D minilungs) and lung bud organoids (3D minilungs). The initial evaluation of a battery of commercially available vitamin D analogs shows a significant reduction in A549 cells of gH2AFX expression levels, a marker of DNA damage, cell senescence and aging.ConclusionsThe treatments based in hypocalcemic vitamin D analogs might be used to reduce the bulk of DNA damage and eventually the subsequent cell senescence expression that underlie lung conditions as those that can evolve with fibrosis.

scholarly journals Sun and Ski Holidays Improve Vitamin D Status, but Are Associated with High Levels of DNA Damage

2014 ◽  
Vol 134 (11) ◽  
pp. 2806-2813 ◽  
Author(s):  
Bibi Petersen ◽  
Hans C. Wulf ◽  
Margarita Triguero-Mas ◽  
Peter A. Philipsen ◽  
Elisabeth Thieden ◽  
...  
Keyword(s):  
Vitamin D ◽  
Dna Damage ◽  
Vitamin D Status

scholarly journals Evidence for Involvement of Nonclassical Pathways in the Protection From UV ‐Induced DNA Damage by Vitamin D–Related Compounds

JBMR Plus ◽  
2021 ◽  
Author(s):  
Warusavithana Gunawardena Manori De Silva ◽  
Jeremy Zhuo Ru Han ◽  
Chen Yang ◽  
Wannit Tongkao‐On ◽  
Bianca Yuko McCarthy ◽  
...  
Keyword(s):  
Vitamin D ◽  
Dna Damage ◽  
Related Compounds

scholarly journals TEMIS UV product validation using NILU-UV ground-based measurements in Thessaloniki, Greece

2017 ◽  
Author(s):  
Melina-Maria Zempila ◽  
Jos H. G. M. van Geffen ◽  
Michael Taylor ◽  
Ilias Fountoulakis ◽  
Maria-Elissavet Koukouli ◽  
...  
Keyword(s):  
Vitamin D ◽  
Dna Damage ◽  
Effective Dose ◽  
Seasonal Pattern ◽  
Functional Relation ◽  
Retrieval Algorithm ◽  
High Temporal Resolution ◽  
Internet Service ◽  
Nonlinear Functional ◽  
Reference Quality

Abstract. This study aims to cross-validate ground-based and satellite-based models of three photobiological UV effective dose products: the CIE erythemal UV, the production of vitamin D in the skin and DNA-damage, using high temporal resolution surface-based measurements of solar UV spectral irradiances from a synergy of instruments and models. The satellite-based Tropospheric Emission Monitoring Internet Service (TEMIS; version 1.4) UV daily dose data products were evaluated over the period 2009 to 2014 with ground-based data from a NILU-UV multifilter radiometer located at the Northern mid-latitude super site of the Laboratory of Atmospheric Physics, Aristotle University of Thessaloniki (LAP/AUTh) in Greece. For the NILU-UV effective dose rates retrieval algorithm, a neural network (NN) was trained to learn the nonlinear functional relation between NILU-UV irradiances and collocated Brewer-based photobiological effective dose products. Then the algorithm was subjected to sensitivity analysis and validation. The correlation of the NN estimates with target outputs was high (r = 0.988 to 0.990) and with a very low bias (0.000 to 0.011 in absolute units) proving the robustness of the NN algorithm. For further evaluation of the NILU NN derived products, retrievals of the vitamin D and DNA-damage effective doses from a collocated YES UVB-1 pyranometer where used. For cloud free days, differences in the derived UV doses are better than 2 % for all UV doses products, revealing the reference quality of the ground-based UV doses at Thessaloniki from the NILU-UV NN retrievals. The TEMIS UV doses used in this study, are derived from ozone measurements by the SCIAMACHY/Envisat and GOME2/MetOp-A satellite instruments, over the European domain in combination with SEVIRI/Meteosat based diurnal cycle of the cloud cover fraction per 0.5° × 0.5° (lat × lon) grid cells. TEMIS UV doses were found to be ~ 12.5 % higher than the NILU NN estimates but, despite the presence of a visually apparent seasonal pattern, the R2 values were found to be robustly high and equal to 0.92–0.93 for 1.588 all sky coincidences. These results significantly improve when limiting the dataset to cloud free days with differences of 0.57 % for the erythemal doses, 1.22 % for the vitamin D doses and 1.18 % for the DNA-damage doses, with standard deviations of the order of 11–13 %. The improvement of the comparative statistics under cloud-free cases further testifies to the importance of the appropriate consideration of the contribution of clouds in the UV radiation reaching the Earth's surface. For the urban area of Thessaloniki with highly variable aerosol the weakness of the implicit aerosol information introduced to the TEMIS UV dose algorithm was revealed by comparison of the datasets to aerosol optical depths at 340 nm as reported by a collocated CIMEL sunphotometer, operating in Thessaloniki at LAP/AUTh as part of the NASA Aerosol Robotic Network.

Does vitamin D protect against DNA damage?

2012 ◽  
Vol 733 (1-2) ◽  
pp. 50-57 ◽  
Author(s):  
Visalini Nair-Shalliker ◽  
Bruce K. Armstrong ◽  
Michael Fenech
Keyword(s):  
Vitamin D ◽  
Dna Damage

scholarly journals The Impact of Vitamin D on Skin Aging

2021 ◽  
Vol 22 (16) ◽  
pp. 9097
Author(s):  
Georgeta Bocheva ◽  
Radomir M. Slominski ◽  
Andrzej T. Slominski
Keyword(s):  
Vitamin D ◽  
Dna Damage ◽  
Nuclear Receptors ◽  
Vitamin D3 ◽  
Skin Aging ◽  
Active Metabolites ◽  
Dna Repair Mechanisms ◽  
Proliferation And Differentiation ◽  
Repair Mechanisms ◽  
The Impact

The active metabolites of vitamin D3 (D3) and lumisterol (L3) exert a variety of antiaging and photoprotective effects on the skin. These are achieved through immunomodulation and include anti-inflammatory actions, regulation of keratinocytes proliferation, and differentiation programs to build the epidermal barrier necessary for maintaining skin homeostasis. In addition, they induce antioxidative responses, inhibit DNA damage and induce DNA repair mechanisms to attenuate premature skin aging and cancerogenesis. The mechanism of action would involve interaction with multiple nuclear receptors including VDR, AhR, LXR, reverse agonism on RORα and -γ, and nongenomic actions through 1,25D3-MARRS receptor and interaction with the nongenomic binding site of the VDR. Therefore, active forms of vitamin D3 including its canonical (1,25(OH)2D3) and noncanonical (CYP11A1-intitated) D3 derivatives as well as L3 derivatives are promising agents for the prevention, attenuation, or treatment of premature skin aging. They could be administrated orally and/or topically. Other forms of parenteral application of vitamin D3 precursor should be considered to avoid its predominant metabolism to 25(OH)D3 that is not recognized by CYP11A1 enzyme. The efficacy of topically applied vitamin D3 and L3 derivatives needs further clinical evaluation in future trials.

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