Effects of purified lignin on in vitro ruminal fermentation and growth performance, carcass traits and fecal shedding of Escherichia coli by feedlot lambs

2009 ◽  
Vol 151 (1-2) ◽  
pp. 21-31 ◽  
Author(s):  
Y. Wang ◽  
T. Marx ◽  
J. Lora ◽  
L.E. Phillip ◽  
T.A. McAllister
2009 ◽  
Vol 89 (2) ◽  
pp. 263-271 ◽  
Author(s):  
J. Baah ◽  
Y. Wang ◽  
T A McAllister

The effect of a direct-fed microbial (DFM) poduct containing a mixed culture of Lactobacillus casei and L. lactis on in vitro ruminal fermentation of barley-grain/barley-silage-based backgrounding and finishing diets and on growth performance and carcass characteristics of feedlot cattle was evaluated during backgrounding (84 d) and finishing (140 d) of 100 Hereford × Angus steers (initial body weight = 280 ± 15.5 kg). The inclusion rates of DFM in the in vitro study were 4, 8, 12 and 16 million colony forming units (CFU) of lactic acid bacteria (LAB) kg-1 DM of substrate. Total in vitro volatile fatty acids (VFA) production increased at 6 and 12 h of incubation (P < 0.01; linear response) when the backgrounding diet was supplemented with DFM. Dry matter digestibility and VFA production also increased (P < 0.05) during a 12-h fermentation of the finishing diet. Steers were randomly allocated to one of four dietary treatments that comprised feeding DFM tp provide 0 (control), 4 × 107, 8 × 107, or 12 × 107 CFU kg-1 diet DM. Average daily gain (ADG, kg) and feed efficiency (G:F; kg gain kg-1 DM consumed) of steers improved (P = 0.002 and 0.001, respectively) as a result of feeding DFM during the backgrounding period, but not during the finishing period. Saleable meat and rib eye area decreased (P = 0.038, linear; and P = 0.041, quadratic) with DFM supplementation. The results indicated that supplementing barley-grain/barley-silage-based feedlot cattle diets with 12 × 107 CFU of the mixed culture of lactobacilli used in these studies could improve ruminal fermentation, ADG and G:F in backgrounding feedlot steers. However, supplementation during the finishing period may not be warranted in terms of growth performance and carcass characteristics. Differences in the microbial ecology of the intestinal tract as a result of differences in diet composition may account for the varied response between the backgrounding and finishing periods.Key words: Beef cattle, direct-fed microbial, growth performance, Lactobacillus casei, Lactobacillus lactis, ruminal fermentation


2018 ◽  
Vol 156 (6) ◽  
pp. 839-847 ◽  
Author(s):  
C. H. S. Rabelo ◽  
E. C. Lara ◽  
F. C. Basso ◽  
C. J. Härter ◽  
R. A. Reis

AbstractWhole-crop maize forage was ensiled without inoculant (control), inoculated with Lactobacillus buchneri and L. plantarum at a rate of 1 × 105 cfu/g fresh forage per bacterium (LBLP), or inoculated with Bacillus subtilis and L. plantarum at a rate 1 × 105 cfu/g fresh forage per bacterium (BSLP) with the goal to investigate the growth performance of finishing feedlot lambs. Thirty Dorper × Santa Ines lambs (29 ± 3.5 kg initial body weight) were used in the feedlot programme and assigned (n = 10) to one of three diets containing control, LBLP or BSLP silages in a 60:40 forage:concentrate ratio. Inoculation of maize silage did not alter dry matter intake (overall mean = 1.16 kg/day) and average daily gain (overall mean = 0.217 kg/day) of lambs. Consequently, feed efficiency remained unchanged. Inoculation of maize silage did not alter carcass and meat traits of lambs, with the exception of meat colour, wherein yellowness (b*) decreased by feeding LBLP and BSLP diets compared with the untreated diet. Regarding ruminal fermentation, there was an interaction between diets and the interval at which ruminal fluid was sampled for determining total volatile fatty acid concentration, but inoculation yielded no obvious results. In conclusion, the use of diets based on maize silage inoculated with L. plantarum combined with either L. buchneri or B. subtilis did not display relevant effects on growth performance of lambs; this response might be related to the limited impact of these bacterial inoculants on silage composition.


2021 ◽  
Vol 42 (6) ◽  
pp. 3399-3414
Author(s):  
Angela Rocio Poveda-Parra ◽  
Odimári Pricila Prado-Calixto ◽  
Elzânia Sales Pereira ◽  
Fernando Luiz Massaro Junior ◽  
Larissa Nóbrega de Carvalho ◽  
...  

The objective of this study was to evaluate ingredients and diets containing increasing levels of crambe cake protein replacing soybean meal protein, with in vitro ruminal fermentation parameters using a gas production technique. Diets were formulated for feedlot lambs and contained different levels of crambe cake protein (0, 250, 500, 750, and 1000 g kg-1) replacing soybean meal protein. Corn silage was used as roughage. Carbohydrate digestion rates were estimated using the in vitro gas production technique and the cumulative gas production kinetics were analyzed using the bicompartmental logistic model. The parameters values of ruminal degradation kinetics were generated using the R statistical program with the Gauss-Newton algorithm and then subjected to analysis of variance and regression (when necessary) according to a completely randomized experimental design with five treatments and five replications. Upon carbohydrate fractionation of ingredients and experimental diets, it was observed that corn grain and corn silage presented the highest levels of total carbohydrates (TC), with values of 128.3 and 464.8 g kg-1 dry matter (DM) in fraction B2, respectively. Lower TC content was found for soybean meal and crambe cake (CC). There was a predominance of fractions A + B1 in the ingredients and experimental diets. The B2 fraction decreased in the diets with the inclusion of the CC protein, and CC presented the highest C fraction. Protein fractionation (g kg-1 DM and g kg-1 crude protein - CP), the ingredients and diets showed a higher proportion of fractions A and B1 + B2. In in vitro degradation, the diet without CC (0 g kg-1 DM) showed the highest final cumulative gas production (365.04 mL g-1 of incubated DM), while the CC presented the lowest volume (166.68 mL g-1 of incubated DM). The gas volume of non-fibrous carbohydrate fermentation and fibrous carbohydrate degradation rate exhibited a quadratic effect according increasing levels of CC (Pmax = 265.8 g kg-1 DM and Pmin = 376.3 g kg-1 DM, respectively). The lag time and final gas volume showed a decreasing linear effect with increasing levels of CC protein. The degradation rate of non-fibrous carbohydrates and the final volume of fibrous carbohydrates did not differ. Replacing soybean meal protein with CC protein at the level of 250 g kg-1 of dry matter in diets formulated for feedlot lambs leads to good profiles of ruminal fermentation kinetics with respect to the degradation of fibrous and non-fibrous carbohydrates.


Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1939
Author(s):  
Thamsanqa Doctor Empire Mpanza ◽  
Abubeker Hassen ◽  
Abiodun Mayowa Akanmu

Feed shortage is the main cause of poor production performance in livestock under smallholder farmer’s in South Africa. Therefore, this study evaluated the growth performance, nutritive value and in vitro ruminal fermentation of Stylosanthes scabra accessions in climatic condition of Pretoria, South Africa as potential forage sources in order to improve feed quality and subsequently livestock production. Stylosanthes scabra accessions were planted in 6 m2 plots following a complete randomized block design with three replicates per accession. The plants were allowed to grow to full maturity after which forage was harvested and yield, chemical composition, phenolic compounds, in vitro organic matter digestibility (IVOMD) and in vitro ruminal fermentation characteristic were determined. Forage yield of accessions ranged between 4.3 to 5.3 t ha−1 in dry matter (DM), and only twelve accessions were identified to be adaptable over the three-year evaluation under rain-fed. Accessions 140, 9281, 11,252, 11,595, 11,604 and 11,625 were consistent in terms of forage yield over the three years. Significant differences among accessions were observed for crude protein (CP), neutral detergent fiber (NDF), IVOMD, metabolizable energy, metabolizable energy yield, gas production, total phenols, total hydrolysable tannins and total condensed tannins. Principal component and clustering analysis showed that accessions 11,255 and 11,625 are distinct in their characteristics as compared to the rest of the accessions, and both accessions are suitable forage source for animals since they contain low NDF with good forage production and crude protein content. However, there is a need for further study to integrate these accessions into the feeding systems in order to improve livestock production.


2013 ◽  
Vol 81 (11) ◽  
pp. 4192-4199 ◽  
Author(s):  
Y N. Nguyen ◽  
Haiqing Sheng ◽  
Rambabu Dakarapu ◽  
John R. Falck ◽  
Carolyn J. Hovde ◽  
...  

ABSTRACTThe human pathogen enterohemorrhagicEscherichia coli(EHEC) O157:H7 colonizes the rectoanal junction (RAJ) in cattle, its natural reservoir. Colonization at the RAJ poses a serious risk for fecal shedding and contamination of the environment. We previously demonstrated that EHEC senses acyl-homoserine lactones (AHLs) produced by the microbiota in the rumen to activate thegadacid resistance genes necessary for survival through the acidic stomachs in cattle and to repress the locus of enterocyte effacement (LEE) genes important for colonization of the RAJ, but unnecessary in the rumen. Devoid of AHLs, the RAJ is the prominent site of colonization of EHEC in cattle. To determine if the presence of AHLs in the RAJ could repress colonization at this site, we engineered EHEC to express theYersinia enterocoliticaAHL synthase geneyenI, which constitutively produces AHLs, to mimic a constant exposure of AHLs in the environment. TheyenI+EHEC produces oxo-C6-homoserine lactone (oxo-C6-HSL) and had a significant reduction in LEE expression, effector protein secretion, and attaching and effacing (A/E) lesion formationin vitrocompared to the wild type (WT). TheyenI+EHEC also activated expression of thegadgenes. To assess whether AHL production, which decreases LEE expression, would decrease RAJ colonization by EHEC, cattle were challenged at the RAJ with WT oryenI+EHEC. Although theyenI+EHEC colonized the RAJ with efficiency equal to that of the WT, there was a trend for the cattle to shed the WT strain longer than theyenI+EHEC.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Robert G. Schaut ◽  
Mitchell V. Palmer ◽  
Paola M. Boggiatto ◽  
Indira T. Kudva ◽  
Crystal L. Loving ◽  
...  

AbstractShiga-toxin producing Escherichia coli O157:H7 (O157)-based vaccines can provide a potential intervention strategy to limit foodborne zoonotic transmission of O157. While the peripheral antibody response to O157 vaccination has been characterized, O157-specific cellular immunity at the rectoanal junction (RAJ), a preferred site for O157 colonization, remains poorly described. Vaccine induced mucosal O157-specific antibodies likely provide some protection, cellular immune responses at the RAJ may also play a role in protection. Distinct lymphoid follicles were increased in the RAJ of vaccinated/challenged animals. Additionally, increased numbers of interferon (IFN)γ-producing cells and γδ + T cells were detected in the follicular region of the RAJ of vaccinated/challenged animals. Likewise, adjuvanted-vaccine formulation is critical in immunogenicity of the O157 parenteral vaccine. Local T cell produced IFNγ may impact epithelial cells, subsequently limiting O157 adherence, which was demonstrated using in vitro attachment assays with bovine epithelial cells. Thus, distinct immune changes induced at the mucosa of vaccinated and challenged animals provide insight of mechanisms associated with limiting O157 fecal shedding. Enhancing mucosal immunity may be critical in the further development of efficacious vaccines for controlling O157 in ruminants and thus limiting O157 transmission to humans.


2010 ◽  
Vol 129 (1-3) ◽  
pp. 135-140 ◽  
Author(s):  
Y. Wang ◽  
A.V. Chaves ◽  
F.L. Rigby ◽  
M.L. He ◽  
T.A. McAllister

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