Utilization of pineapple stem juice to enhance enzyme-hydrolytic efficiency for sugarcane bagasse after an optimized pre-treatment with alkaline peroxide

Lespedeza crytobotrya is a shrub species with properties of substantial biomass and widely distributes in the desert region of China. The cellulose separated from Lespedeza after pre-treatment can be enzymatic hydrolyzed into glucose for ethanol or other chemicals production, which are important renewable fuels or raw material for other material synthesis. Moreover it also can be used for cellulosic material production. So it is necessary to evaluate the cellulose of Lespedeza crytobotrya before its utilization. In this study four cellulosic fractions were isolated by pretreatment with low severity steam and post-treatment with alkaline peroxide. They were comparatively studied by sugar analysis and the average degree of polymerization. After alkaline peroxide post-treatment, the hemicelluloses in the cellulosic fractions were removed markedly. The treatment intensity had a profound effect on the average degree of polymerization, which was increased firstly and then decreased. A combination of low severity steam pretreatment and alkaline peroxide post-treatment is an effective method for Lespedeza stalks to obtain high glucose yield.

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High-pressure carbon dioxide/water pre-treatment of sugarcane bagasse and elephant grass: Assessment of the effect of biomass composition on process efficiency

Bioresource Technology ◽

10.1016/j.biortech.2016.11.101 ◽

2017 ◽

Vol 224 ◽

pp. 639-647 ◽

Cited By ~ 29

Author(s):

Andréia Toscan ◽

Ana Rita C. Morais ◽

Susana M. Paixão ◽

Luís Alves ◽

Jürgen Andreaus ◽

...

Keyword(s):

Carbon Dioxide ◽

High Pressure ◽

Sugarcane Bagasse ◽

Process Efficiency ◽

Biomass Composition ◽

Elephant Grass ◽

Pre Treatment

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Extraction and characterisation of cellulose nanocrystals (CNCs) from sugarcane bagasse using ionic liquids

10.51415/10321/3219 ◽

2019 ◽

Author(s):

◽

Gcinile Pretty Mdletshe

Keyword(s):

Electron Microscopy ◽

Ionic Liquids ◽

Sugarcane Bagasse ◽

Cellulose Nanocrystals ◽

Crystallinity Index ◽

Attenuated Total Reflection ◽

X Ray Diffraction ◽

X Ray ◽

Transmission Electron ◽

Pre Treatment

Lignocellulosic materials have the potential to partly replace fossil-based resources as a source of bio-fuels, bio-chemicals, bio-composites and other bio-products. In this study, ionic liquids (ILs) were used in the pre-treatment of ground sugarcane bagasse (SCB). The ILs used were 1-butyl-3-methylimidazolium hydrogen sulphate or 1-butyl-3-methylimidazolium methyl sulphate at varied times. The ILs were able to remove lignin and hemicellulose from biomass. The IL [bmim][HSO4] had the highest amount of lignin removed after 12 h than all samples. Moreover, it resulted in the greatest cellulose amount. Milled SCB was pre-treated with IL/dimethyl sulphoxide (DMSO) mixtures. The IL [bmim][HSO4] was able to produce cellulose nanocrystals (CNCs) at 90 % IL and 100 % IL. The other IL failed to produce CNCs. Freeze drying the CNC suspension showed morphologies of long fibrous structures and rods which were evident in the scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images. The crystallinity index of cellulose in the form of CNCs was calculated from powder X-ray diffraction (P-XRD). Thermal analysis of the CNCs was obtained from thermogravimetric analysis (TGA). Attenuated total reflection-Fourier transform infrared (ATR-FTIR) was used to confirm the absence of lignin and hemicellulose in CNCs. The size distribution of CNCs was obtained by using a dynamic light scattering (DLS) which showed that all the CNCs for the 100 % IL [bmim][HSO4] pre-treatment had a length < 500 nm. It was found that [bmim][HSO4], with no DMSO, was the most effective in terms of cellulose dissolution and the crystal sizes of CNCs. The conversion of cellulose to CNCs was successful with a 80 % and 100 % conversion for 90 % [bmim][HSO4]/DMSO and 100 % [bmim][HSO4], respectively.

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Effective fermentation of sugarcane bagasse whole slurries using robust xylose-capable Saccharomyces cerevisiae

10.22541/au.163319410.08076047/v1 ◽

2021 ◽

Author(s):

Thapelo Mokomele ◽

Bianca Brandt ◽

Johann Görgens

Keyword(s):

Saccharomyces Cerevisiae ◽

Sugarcane Bagasse ◽

Initial Screening ◽

Fed Batch ◽

Xylose Consumption ◽

Microbial Inhibitors ◽

Ethanol Yields ◽

Pre Treatment ◽

Whole Slurry ◽

Simultaneous Saccharification

The pre-treatment of lignocellulose material toward cellulosic bioethanol production releases microbial inhibitors that severely limit the fermentation ability of Saccharomyces cerevisiae. This study evaluated to what degree robust xylose capable strains may improve the fermentability of non-detoxified sugarcane bagasse (SCB) slurries derived from steam explosion (StEX), and further compared this to slurries derived from ammonia fibre expansion (AFEX) pre-treatment. Initial screening in separate hydrolyses and co-fermentation processes using StEx-SCB hydrolysates identified S. cerevisiae TP-1 and CelluXTM4 with higher xylose consumption (≥ 88%) and ethanol concentrations (≥ 50 g/L). Subsequent fermentations compared StEx and AFEX pre-treated SCB material under industrially relevant fed-batch pre-hydrolysis simultaneous saccharification and co-fermentation (PSSCF) conditions, which resulted in only 3 g/L differences in ethanol titres for StEx and AFEX PSSCF fermentations. The study achieved non-detoxified whole-slurry co-fermentation using StEx pre-treated SCB, with higher ethanol yields than previously reported, by utilising robust xylose-capable strains.

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