Effect of lime pre-treatment on the synergistic hydrolysis of sugarcane bagasse by hemicellulases

2010 ◽  
Vol 101 (12) ◽  
pp. 4472-4478 ◽  
Author(s):  
Natasha Beukes ◽  
Brett I. Pletschke
Keyword(s):  
Sugarcane Bagasse ◽  
Pre Treatment ◽  
Hydrolysis Of

scholarly journals Insights into the genome and secretome of Fusarium metavorans DSM105788 by cultivation on agro-residual biomass and synthetic nutrient sources

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Sophie C. Brandt ◽  
Hévila Brognaro ◽  
Arslan Ali ◽  
Bernhard Ellinger ◽  
Katharina Maibach ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
High Performance ◽  
Waste Products ◽  
Promising Alternative ◽  
Nutrient Sources ◽  
Cellulose Substrates ◽  
Enzyme Cocktail ◽  
Maize Leaves ◽  
Pre Treatment ◽  
Hydrolysis Of

Abstract Background The transition to a biobased economy involving the depolymerization and fermentation of renewable agro-industrial sources is a challenge that can only be met by achieving the efficient hydrolysis of biomass to monosaccharides. In nature, lignocellulosic biomass is mainly decomposed by fungi. We recently identified six efficient cellulose degraders by screening fungi from Vietnam. Results We characterized a high-performance cellulase-producing strain, with an activity of 0.06 U/mg, which was identified as a member of the Fusarium solani species complex linkage 6 (Fusarium metavorans), isolated from mangrove wood (FW16.1, deposited as DSM105788). The genome, representing nine potential chromosomes, was sequenced using PacBio and Illumina technology. In-depth secretome analysis using six different synthetic and artificial cellulose substrates and two agro-industrial waste products identified 500 proteins, including 135 enzymes assigned to five different carbohydrate-active enzyme (CAZyme) classes. The F. metavorans enzyme cocktail was tested for saccharification activity on pre-treated sugarcane bagasse, as well as untreated sugarcane bagasse and maize leaves, where it was complemented with the commercial enzyme mixture Accellerase 1500. In the untreated sugarcane bagasse and maize leaves, initial cell wall degradation was observed in the presence of at least 196 µg/mL of the in-house cocktail. Increasing the dose to 336 µg/mL facilitated the saccharification of untreated sugarcane biomass, but had no further effect on the pre-treated biomass. Conclusion Our results show that F. metavorans DSM105788 is a promising alternative pre-treatment for the degradation of agro-industrial lignocellulosic materials. The enzyme cocktail promotes the debranching of biopolymers surrounding the cellulose fibers and releases reduced sugars without process disadvantages or loss of carbohydrates.

Biomass Processing Routes for Production of Raw Materials with High Added Value

2015 ◽  
pp. 241-273
Author(s):  
Rubens Maciel Filho ◽  
Laura Plazas Tovar ◽  
Yurany Camacho Ardila ◽  
Jaiver Efrén Jaimes Figueroa ◽  
Maria Regina Wolf Maciel
Keyword(s):  
Sugarcane Bagasse ◽  
Raw Materials ◽  
Raw Material ◽  
Added Value ◽  
Biochemical Processes ◽  
2Nd Generation ◽  
Biomass Processing ◽  
Thermochemical Processes ◽  
Pre Treatment ◽  
Hydrolysis Of

In this chapter sugarcane bagasse may be submitted to a biological route in which the technologies used to obtain lignocellulosic ethanol (2nd generation ethanol) from lignocellulosic materials involve pre-treatment and the hydrolysis of the polysaccharides in the biomass into fermentable sugars for subsequent fermentation. Taking into consideration the use of sugarcane bagasse as a raw material for 2nd generation ethanol, the acid hydrolysis / pretreatment of sugarcane bagasse could be more feasible that others, and must be evaluated in this context. On the other hand, from biomass is possible to obtain products with high added value and energy, mainly by the use of thermochemical processes (e.g. pyrolysis and gasification) and biochemical processes (e.g., fermentation and anaerobic digestion). However, the products obtained from the thermochemical processes can be used as raw material for biochemical processes which multiplies the quantity of products to be obtained from biomass.

Enzyme-assisted Extraction for Optimized Recovery of Phenolic Bioactives from Peganum hermala Leaves Using Response Surface Methodology

2018 ◽  
Vol 17 (4) ◽  
pp. 349-354
Author(s):  
Qadir Rahman ◽  
Anwar Farooq ◽  
Amjad Gilani Mazhar ◽  
Nadeem Yaqoob Muhammad ◽  
Ahmad Mukhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enzyme Concentration ◽  
Coumaric Acid ◽  
Assisted Extraction ◽  
Pre Treatment ◽  
Optimized Conditions ◽  
Enzyme Complexes ◽  
Hydrolysis Of ◽  
Central Composite

This study investigates the effect of enzyme formulations (Zympex-014, Kemzyme dry-plus and Natuzyme) on recovery of phenolics from Peganum hermala (harmal) leaves, under optimized conditions using response surface methodology. As compared to the other enzyme complexes, the yield (34 g/100g) obtained through Zympex-014-assisted extraction was higher under optimized conditions such as time (75 min), temperature (70°C), pH (6.5) and enzyme concentration (5 g/100 g) using central composite design (CCD). Effectiveness of Zympex-014 towards hydrolysis of P. hermala leaves cell wall was examined by analyzing the control and enzyme-treated leave residues using scanning electron microscope (SEM). GC/MS characterization authenticated the presence of quercetin (1.44), gallic acid (0.23), caffeic acid (0.04), cinnamic acid (0.05), m-coumaric acid (0.23) and p-coumaric acid (0.37 μg/g) as the potent phenolics in Zympex-014 based extract. It can be concluded from the findings of the current work that pre-treatment of P. hermala leaves with Zympex-014 significantly enhanced the recovery of phenolics that supports its potential uses in the nutra-pharamaceutical industry.

A combination of mild chemical pre-treatment and enzymatic hydrolysis efficiently produces xylooligosaccharides from sugarcane bagasse

2021 ◽  
Vol 291 ◽  
pp. 125972
Author(s):  
Shuai Zhao ◽  
Gui-Ling Zhang ◽  
Chen Chen ◽  
Qi Yang ◽  
Xue-Mei Luo ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Pre Treatment

The Effect of Xylan Removal on the High-Solid Enzymatic Hydrolysis of Sugarcane Bagasse

2021 ◽  
Author(s):  
Leidy Patricia Quintero ◽  
Nathalia P. Q. de Souza ◽  
Adriane M. F. Milagres
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
High Solid ◽  
Hydrolysis Of

scholarly journals A novel recyclable furoic acid-assisted pretreatment for sugarcane bagasse biorefinery in co-production of xylooligosaccharides and glucose

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lin Dai ◽  
Tian Huang ◽  
Kankan Jiang ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Production Cost ◽  
Solid Loading ◽  
Fed Batch ◽  
Acid Pretreatment ◽  
Furoic Acid ◽  
Cooling Crystallization ◽  
Mineral Acids ◽  
Hydrolysis Of

Abstract Background Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 °C for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization. Conclusion This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.

scholarly journals The effect of drying temperature on the properties of gelatin from carps (Cyprinus carpio) skin

2019 ◽  
Vol 37 (No. 4) ◽  
pp. 246-251 ◽  
Author(s):  
Joanna Tkaczewska ◽  
Maciej Wielgosz ◽  
Piotr Kulawik ◽  
Marzena Zajac
Keyword(s):  
Cyprinus Carpio ◽  
Freeze Drying ◽  
Treatment Method ◽  
Thermal Hydrolysis ◽  
Gel Properties ◽  
Drying Temperature ◽  
Freeze Dried ◽  
Different Temperatures ◽  
Pre Treatment ◽  
Hydrolysis Of

The influence of drying temperature on the characteristics and gel properties of gelatine from Cyprinus carpio L. skin was studied. Gelatine was extracted from the carp skin using NaOH and ethanol pre-treatment method, extracted in water in 45°C and then dried in 4 different temperatures: 50, 70, 80°C and freeze-dried. The  electrophoresis and functional properties of gelatines were investigated. Freeze drying allowed to obtain a high gelling force, and all other methods did not give satisfactory results. The proteins in gelatines dried at higher temperatures separated by electrophoresis gave severely blurred bands. It may be explained by thermal hydrolysis of collagen fibrils. Freeze drying is the only effective method for drying this product, which can be used in industry.

Evaluation and development of a novel pre-treatment method for mulberry leaves to enhance their bioactivity via enzymatic degradation of GAL-DNJ to DNJ

2021 ◽  
Author(s):  
Soo Takasu ◽  
Isabella Supardi Parida ◽  
Yoshihiro Kojima ◽  
Toshiyuki Kimura ◽  
Kiyotaka Nakagawa
Keyword(s):  
Enzymatic Degradation ◽  
Treatment Method ◽  
Mulberry Leaves ◽  
Pre Treatment ◽  
Hydrolysis Of

We demonstrated the conversion of GAL-DNJ into DNJ improves mulberry leaves bioavailability and constructed a novel enzymatic-based method to induce the hydrolysis of GAL-DNJ to DNJ.

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