Microbial and hydrolase activity after release of indoleacetic acid and ethylene–polyamine precursors by a model root surface

2011 ◽  
Vol 47 (2) ◽  
pp. 106-110 ◽  
Author(s):  
Giancarlo Renella ◽  
Loretta Landi ◽  
Jose M. Garcia Mina ◽  
Laura Giagnoni ◽  
Paolo Nannipieri
1986 ◽  
Vol 64 (3) ◽  
pp. 573-578 ◽  
Author(s):  
F. G. Albert ◽  
L. W. Bennett ◽  
A. J. Anderson

The surface of bean roots demonstrates an intense peroxidase activity which was detected by hydrogen peroxide dependent formation of chromogen from chloronaphthol or dianisidine. Other peroxidase functions, oxidation of indoleacetic acid and NADPH, were catalysed by intact roots and were stimulated by Mn2+ and p-coumarate. Oxidation of NADPH involved superoxide anion [Formula: see text] and hydrogen peroxide formation. Molecular sizing chromatography of root washes demonstrated NADPH oxidase and peroxidase to be associated with higher weight components than indoleacetic acid oxidase. Root surface and root wash peroxidase displayed optimal activity between pH 7 and 8, whereas both sources of indoleacetic acid oxidase were more active at acidic pH. Native poly aery lamide gel electrophoresis of sterile root washes displayed two fast-moving anodic bands, whereas homogenates of the plant roots had several slower moving bands in addition.


2014 ◽  
Vol 1 (3) ◽  
pp. 57-61
Author(s):  
E. Kopylov

Aim. To study the specifi cities of complex inoculation of spring wheat roots with the bacteria of Azospirillum genus and Chaetomium cochliodes Palliser 3250, and the isolation of bacteria of Azospirillum genus, capable of fi xing atmospheric nitrogen, from the rhizospheric soil, washed-off roots and histoshere. Materials and meth- ods. The phenotypic features of the selected bacteria were identifi ed according to Bergi key. The molecular the polymerase chain reaction and genetic analysis was used for the identifi cation the bacteria. Results. It has been demonstrated that during the introduction into the root system of spring wheat the strain of A. brasilensе 102 actively colonizes rhizospheric soil, root surface and is capable of penetrating into the inner plant tissues. Conclusions. The soil ascomucete of C. cochliodes 3250 promotes better settling down of Azospirillum cells in spring wheat root zone, especially in plant histosphere which induces the increase in the content of chlorophyll a and b in the leaves and yield of the crop.


2019 ◽  
Vol 31 (1) ◽  
pp. 31-36
Author(s):  
Monya N. Hassan ◽  
Maha A. Aziz

Background: Gingival recession is defined as the apical migration of the gingival margin below the cementoenamel junction (CEJ), resulting in exposure of the root surface and it is one of the main esthetic complaints of patients. The management of gingival recession and its sequelae is based on a thorough assessment of the etiological factors and the degree of tissue involvement.


2009 ◽  
Vol 62 ◽  
pp. 402-402
Author(s):  
S.L. Lewthwaite ◽  
P.J. Wright

The predominant diseases of the commercial kumara (Ipomoea batatas) or sweetpotato crop are caused by fungal pathogens The field disease pink rot results from infection by the fungus Sclerotinia sclerotiorum Lesions form on vines but may spread down stems to the roots The widespread nature of this disease in sweetpotato appears peculiar to New Zealand Scurf is a disease caused by Monilochaetes infuscans which occurs in the field but may proliferate amongst stored roots The disease causes a superficial discolouration of the root surface which is mainly cosmetic but can also increase root water loss in storage Infection by Ceratocystis fimbriata produces a disease known as black rot The disease can be transmitted amongst plants at propagation but is particularly rampant amongst roots in storage This disease is readily transmitted and can cause severe economic loss Fusarium oxysporum causes surface rots in stored roots characterised by light to dark brown lesions that tend to be firm dry and superficial The lesions may be circular and centred on wounds caused by insects or mechanical damage at harvest Soft rot caused by Rhizopus stolonifer generally occurs in roots after they are washed and prepared for the market Fungal infection occurs through wounds or bruised tissue producing distinctive tufts of white fungal strands and black spores


2010 ◽  
Vol 18 (6) ◽  
pp. 1151-1156 ◽  
Author(s):  
Jian-Kai SHEN ◽  
Huang HUANG ◽  
Zhi-Qiang FU ◽  
Yang-Zhu ZHANG ◽  
Pan LONG ◽  
...  

1989 ◽  
Vol 171 (2) ◽  
pp. 708-713 ◽  
Author(s):  
W Q Xie ◽  
B A Whitton ◽  
J W Simon ◽  
K Jäger ◽  
D Reed ◽  
...  

2021 ◽  
Vol 9 (8) ◽  
pp. 1647
Author(s):  
Gui-E Li ◽  
Wei-Liang Kong ◽  
Xiao-Qin Wu ◽  
Shi-Bo Ma

Phytase plays an important role in crop seed germination and plant growth. In order to fully understand the plant growth-promoting mechanism by Rahnella aquatilis JZ-GX1,the effect of this strain on germination of maize seeds was determined in vitro, and the colonization of maize root by R. aquatilis JZ-GX1 was observed by scanning electron microscope. Different inoculum concentrations and Phytate-related soil properties were applied to investigate the effect of R. aquatilis JZ-GX1 on the growth of maize seedlings. The results showed that R. aquatilis JZ-GX1 could effectively secrete indole acetic acid and had significantly promoted seed germination and root length of maize. A large number of R. aquatilis JZ-GX1 cells colonized on the root surface, root hair and the root interior of maize. When the inoculation concentration was 107 cfu/mL and the insoluble organophosphorus compound phytate existed in the soil, the net photosynthetic rate, chlorophyll content, phytase activity secreted by roots, total phosphorus concentration and biomass accumulation of maize seedlings were the highest. In contrast, no significant effect of inoculation was found when the total P content was low or when inorganic P was sufficient in the soil. R. aquatilis JZ-GX1 promotes the growth of maize directly by secreting IAA and indirectly by secreting phytase. This work provides beneficial information for the development and application of R. aquatilis JZ-GX1 as a microbial fertilizer in the future.


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