Long-term seed storage and viability of Zostera marina

The National Tree Seed Centre has been providing seed of known origin and quality for research for 40 years. Seed is also stored for long-term gene conservation purposes to provide a source of germplasm for future research and restoration. This is particularly important for species facing such threats as insect attack, disease, climate change, or conversion of forest land to non-forest uses. The Centre's inventory focuses on native tree and shrub species, striving to store samples from throughout their ranges. Over 26 000 seed samples have been sent to researchers in 65 countries, 70% of these samples being distributed within Canada. Seed research has always been a component of the Seed Centre's program. One notable accomplishment is the development of the Petawawa Germination Box. The Seed Centre participates in and contributes to activities of the Association of Official Seed Analysts, the International Seed Testing Association, and the IUFRO Seed Physiology and Technology Research Group. Key words: collection, dormancy, ex situ gene conservation, germination, research, seed, storage

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Impact of ocean carbonation on long-term regulation of light harvesting in eelgrass Zostera marina

Marine Ecology Progress Series ◽

10.3354/meps13777 ◽

2021 ◽

Author(s):

B Celebi-Ergin ◽

RC Zimmerman ◽

VJ Hill

Keyword(s):

Zostera Marina ◽

Light Harvesting

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Mid storage seed hardening: a mechanical method to maintain seed viability during long term jute seed preservation

Plant Science Today ◽

10.14719/pst.2021.8.1.916 ◽

2021 ◽

Vol 8 (1) ◽

pp. 79-88

Author(s):

Md. Nasir Uddin ◽

S. M. Mahbub Ali ◽

Md. Abu Sadat ◽

Md Amazed Hossain Chowdhury ◽

Israt Jahan Mumu ◽

...

Keyword(s):

Crop Production ◽

Agricultural Sector ◽

Seed Viability ◽

Early Period ◽

Chemical Properties ◽

Seed Storage ◽

Germination Percentage ◽

Oil Degradation ◽

Hardening Treatment

Seed plays an important role in agricultural sector for both production and consumption purpose. Availability of vigour seed is one of the major constraints for maximizing crop production. However, healthy seed can also lose its viability during seed storage by changing different physio-chemical properties. Influence of environmental factors and seed containers during storage leading to seed deterioration. In this research, mid storage seed hardening treatment was applied in different aged seeds of jute species (C. Capsularis & C. olitorius) with two types of storage bags. Seed hardening treatment showed the less moisture content with better germination percentage compared to the untreated species of jute seeds. Seed packing in polythene bags during both short and long term seed storages had higher viable seeds compared to the cloth packing seeds. The effect of seed hardening treatment on seed oil content and pattern of oil degradation is distinct in early period of storage. The faster rate of oil degradation, soluble protein and free amino acids was found in seeds of un-treated stored seeds in cloth bag. Contrary, very slow rate of oil degradation was observed in harden seed and stored in polythene bag which indicated better storability of harden seeds.

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Relationship between mitochondrial changes and seed aging as a limitation of viability for the storage of beech seed (Fagus sylvatica L.)

PeerJ ◽

10.7717/peerj.10569 ◽

2021 ◽

Vol 9 ◽

pp. e10569

Author(s):

Arleta Małecka ◽

Liliana Ciszewska ◽

Aleksandra Staszak ◽

Ewelina Ratajczak

Keyword(s):

Fagus Sylvatica ◽

Molecular Mechanisms ◽

Membrane Integrity ◽

Natural Aging ◽

Seed Storage ◽

Biological Processes ◽

Embryonic Axes ◽

Seed Aging ◽

Fagus Sylvatica L

Aging is one of the most fundamental biological processes occurring in all forms of eukaryotic life. Beech trees (Fagus sylvatica L.) produce seeds in intervals of 5–10 years. Its yearly seed yield is usually very low, so there is a need for long-term seed storage to enable propagation of this species upon demand. Seeds for sowing must be of high quality but they are not easy to store without viability loss. Understanding the mechanism responsible for seed aging is therefore very important. We observed the generation of reactive oxygen species (ROS) in mitochondria of embryonic axes and cotyledons of beech seeds during natural aging. The presence of ROS led to changes in compromised mitochondrial membrane integrity and in mitochondrial metabolism and morphology. In this study, we pointed to the involvement of mitochondria in the natural aging process of beech seeds, but the molecular mechanisms underlying this involvement are still unknown.

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Seed germination evaluation of Phalaenopsis amabilis in various media for long-term conservation

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d221162 ◽

2021 ◽

Vol 22 (11) ◽

Author(s):

DWI MURTI PUSPITANINGTYAS ◽

ELIZABETH HANDINI

Keyword(s):

Regression Analysis ◽

Seed Germination ◽

Life Span ◽

Seed Storage ◽

Optimum Conditions ◽

Presidential Decree ◽

Orchid Seed ◽

Leaf Fertilizer

Abstract. Puspitaningtyas DM, Handini E. 2021. Seed germination evaluation of Phalaenopsis amabilis in various media for long-term conservation. Biodiversitas 22: 5231-5238. Phalaenopsis amabilis (L.) Blume (moon orchid) is one of Indonesia's national flowers, which is mentioned in Presidential Decree No. 4/1993. Study on the orchid seed of P. amabilis was conducted to observe the longevity of seeds storage. The purpose of this study was to determine the viability of P. amabilis seeds after storage for years, then to predict the life span of the seeds, and to find out the best media germination for P. amabilis seeds. After harvesting, P. amabilis seeds were stored at –20 °C and the germination tests were carried out periodically in different periods until the seeds lose their viability to determine the optimum conditions for long-term seed storage. Four trial media cultures have been used to test seed germination by means of in vitro culture. The result showed that seeds of P. amabilis were able to germinate in 4 media i.e. Knudson C, modified Knudson C, modified Vacin & Went and modified leaf fertilizer. The best germination was on leaf fertilizer medium. Based on the research showed that the viability seeds of P. amabilis decreased sharply after 6 years stored. By using regression analysis, the life span of the seeds could be predicted can reach 9 years of storage which is sowing on the best media (leaf fertilizer).

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Pest control strategies and damage potential of seed-infesting pests in the Czech stores – a review

Plant Protection Science ◽

10.17221/10/2014-pps ◽

2014 ◽

Vol 50 (No. 4) ◽

pp. 165-173 ◽

Cited By ~ 22

Author(s):

V. Stejskal ◽

R. Aulicky ◽

Z. Kucerova

Keyword(s):

Control Strategies ◽

Significant Risk ◽

Seed Storage ◽

Economic Damage ◽

Damage Potential ◽

The Czech Republic ◽

Modified Atmospheres ◽

New Research ◽

Wheat Kernels

This work reviews the historical and current pest risks and research concerning seed storage in the Czech Republic (CR). Stored seed pests (i.e. animals causing injuries to the germ and endosperm) represent a high risk of economic damage due to the high value of seeds coupled with long-term seed storage in small storage units (e.g., boxes, satchels). Rodents represent a significant risk to all types of seeds, especially seeds stored in piles or bags. Mites, psocids, and moths are the main pests of stored grass and vegetable seeds: mites can decrease seed germinability by 52% and psocids caused 9.7% seed weight loss in broken wheat kernels after 3 months of infestation under laboratory conditions. Although beetles (Sitophilus sp., Tribolium sp., Oryzaephilus sp.) and moths (Plodia sp.) are common pests of grain seeds (e.g., wheat, barley, maize), two serious seed pests, Sitotroga cereallela and S. zemays, are rare in the CR. Bruchus pisorum is a common pest of pea seeds, while other Bruchids are rare in the Czech legume seed stores. Currently, the control of seed pests is becoming difficult because the efficient pesticides (e.g., methylbromide, dichlorvos, drinking anticoagulant rodent baits) for seed protection have been lost without the development of adequate substitutes. New research on seed protection in the CR using biological control (mite predators Cheyletus sp.), low pressure, modified atmospheres, and hydrogen cyanide is overviewed.  

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Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration

Plant Methods ◽

10.1186/s13007-019-0541-6 ◽

2019 ◽

Vol 15 (1) ◽

Cited By ~ 1

Author(s):

Shidong Yue ◽

Yu Zhang ◽

Yi Zhou ◽

Shaochun Xu ◽

Shuai Xu ◽

...

Keyword(s):

Seed Storage ◽

Storage Conditions ◽

Seed Vigor ◽

Ex Situ ◽

Zostera Japonica ◽

Storage Method ◽

Seagrass Meadows ◽

Long Term Storage ◽

Term Storage

Abstract Background Seagrass meadows are recognized as critical and among the most vulnerable habitats on the planet. The alarming rates of decline in seagrass meadows have attracted the attention globally. There is an urgent need to develop techniques to restore and preserve these vital coastal ecosystems. So far little work has been done to develop effective long-term storage method for seagrass seeds. The seagrass Zostera japonica Asch. & Graebn is an endangered species in its native range. Here we utilized combinations of different storage times, salinities, and temperature to determine the most appropriate conditions for optimal seed storage. Results Zostera japonica seeds were strongly desiccation sensitive, with a complete loss of viability after 24 h of desiccation. Therefore, long periods of exposure to air should be avoided to minimize seed mortality. In addition, Z. japonica seeds could not endure freezing conditions such as – 5 °C. However, our results indicated that reduced storage temperature to 0 °C could effectively prolong the duration of dormancy of Z. japonica seeds. Seeds stored at 0 °C under a salinity of 40–60 psu showed relatively low seed loss, high seed vigor and fast seed germination, suggesting these to be optimal seed storage conditions. For example, after storage for 540 days (ca. 600 days since the seed collection from reproductive shoots in early October, 2016) at 0 °C under a salinity of 50 psu, seeds still had a considerable vigor, i.e. 57.8 ± 16.8%. Conclusion Our experiments demonstrated that seeds stored at 0 °C under a salinity of 40–60 psu could effectively prolong the duration of dormancy of Z. japonica seeds. The proposed technique is a simple and effective long-term storage method for Z. japonica seeds, which can then be used to aid future conservation, restoration and management of these sensitive and ecologically important habitat formers. The findings may also serve as useful reference for seed storage of other threatened seagrass species and facilitate their ex situ conservation and habitat restoration.

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