scholarly journals IncK plasmid-mediated tetracycline resistance in Edwardsiella ictaluri isolates from diseased freshwater catfish in Vietnam

Aquaculture ◽  
2009 ◽  
Vol 295 (3-4) ◽  
pp. 157-159 ◽  
Author(s):  
Tu Thanh Dung ◽  
Freddy Haesebrouck ◽  
Patrick Sorgeloos ◽  
Nguyen Ahn Tuan ◽  
Frank Pasmans ◽  
...  
Keyword(s):  
Tetracycline Resistance ◽  
Edwardsiella Ictaluri ◽  
Freshwater Catfish

scholarly journals Genomic analysis of group B Streptococcus from milk demonstrates the need for improved biosecurity: a cross-sectional study of pastoralist camels in Kenya

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dinah Seligsohn ◽  
Chiara Crestani ◽  
Taya L. Forde ◽  
Erika Chenais ◽  
Ruth N. Zadoks
Keyword(s):  
Public Health ◽  
Milk Production ◽  
Disease Transmission ◽  
Tetracycline Resistance ◽  
Genomic Analysis ◽  
Sub Saharan Africa ◽  
Camel Milk ◽  
Interspecies Transmission ◽  
Cross Sectional ◽  
Group B

Abstract Background Streptococcus agalactiae (Group B Streptococcus, (GBS)) is the leading cause of mastitis (inflammation of the mammary gland) among dairy camels in Sub-Saharan Africa, with negative implications for milk production and quality and animal welfare. Camel milk is often consumed raw and presence of GBS in milk may pose a public health threat. Little is known about the population structure or virulence factors of camel GBS. We investigated the molecular epidemiology of camel GBS and its implications for mastitis control and public health. Results Using whole genome sequencing, we analysed 65 camel milk GBS isolates from 19 herds in Isiolo, Kenya. Six sequence types (STs) were identified, mostly belonging to previously described camel-specific STs. One isolate belonged to ST1, a predominantly human-associated lineage, possibly as a result of interspecies transmission. Most (54/65) isolates belonged to ST616, indicative of contagious transmission. Phylogenetic analysis of GBS core genomes showed similar levels of heterogeneity within- and between herds, suggesting ongoing between-herd transmission. The lactose operon, a marker of GBS adaptation to the mammary niche, was found in 75 % of the isolates, and tetracycline resistance gene tet(M) in all but two isolates. Only the ST1 isolate harboured virulence genes scpB and lmb, which are associated with human host adaptation. Conclusions GBS in milk from Kenyan camel herds largely belongs to ST616 and shows signatures of adaptation to the udder. The finding of similar levels of within- and between herd heterogeneity of GBS in camel herds, as well as potential human-camel transmission highlights the need for improved internal as well as external biosecurity to curb disease transmission and increase milk production.

scholarly journals Identification and Transferability of Tetracycline Resistance in Streptococcus thermophilus during Milk Fermentation, Storage, and Gastrointestinal Transit

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 65
Author(s):  
Armin Tarrah ◽  
Shadi Pakroo ◽  
Viviana Corich ◽  
Alessio Giacomini
Keyword(s):  
Efflux Pump ◽  
Acquired Resistance ◽  
Gastrointestinal Transit ◽  
Tetracycline Resistance ◽  
Lactobacillus Delbrueckii ◽  
Genomic Islands ◽  
Resistant Bacteria ◽  
Human Pathogens ◽  
Resistance Level ◽  
Milk Fermentation

The existence of antibiotic-resistant bacteria in food products, particularly those carrying acquired resistance genes, has increased concerns about the transmission of these genes from beneficial microbes to human pathogens. In this study, we evaluated the antibiotic resistance-susceptibility patterns of 16 antibiotics in eight S. thermophilus strains, whose genome sequence is available, using phenotypic and genomic approaches. The minimal inhibitory concentration values collected revealed intermediate resistance to aminoglycosides, whereas susceptibility was detected for different classes of β-lactams, quinolones, glycopeptide, macrolides, and sulfonamides in all strains. A high tetracycline resistance level has been detected in strain M17PTZA496, whose genome analysis indicated the presence of the tet(S) gene and the multidrug and toxic compound extrusion (MATE) family efflux pump. Moreover, an in-depth genomic analysis revealed genomic islands and an integrative and mobilizable element (IME) in the proximity of the gene tet(S). However, despite the presence of a prophage, genomic islands, and IME, no horizontal gene transfer was detected to Lactobacillus delbrueckii subsp. lactis DSM 20355 and Lactobacillusrhamnosus GG during 24 h of skim milk fermentation, 2 weeks of refrigerated storage, and 4 h of simulated gastrointestinal transit.

scholarly journals Validation of Edwardsiella ictaluri oral vaccination platform in experimental pond trials

2019 ◽  
Vol 51 (2) ◽  
pp. 346-363 ◽  
Author(s):  
David J. Wise ◽  
Terrence E. Greenway ◽  
Todd S. Byars ◽  
Ganesh Kumar ◽  
Matt J. Griffin ◽  
...  
Keyword(s):  
Edwardsiella Ictaluri ◽  
Oral Vaccination ◽  
Experimental Pond

scholarly journals Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 288
Author(s):  
Sonia Sciortino ◽  
Pietro Arculeo ◽  
Vincenzina Alio ◽  
Cinzia Cardamone ◽  
Luisa Nicastro ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Tetracycline Resistance ◽  
Multi Drug Resistance ◽  
Genetic Determinants ◽  
Antibiotic Resistant ◽  
Resistance Profile ◽  
Antimicrobial Resistance Profile ◽  
Drug Resistance Profile ◽  
Arcobacter Butzleri ◽  
Seawater Samples

Arcobacter spp. are emerging waterborne and foodborne zoonotic pathogens responsible for gastroenteritis in humans. In this work, we evaluated the occurrence and the antimicrobial resistance profile of Arcobacter isolates recovered from different aquatic sources. Besides, we searched for Arcobacter spp. in seaweeds and the corresponding seawater samples. Bacteriological and molecular methods applied to 100 samples led to the isolation of 28 Arcobacter isolates from 27 samples. The highest prevalence was detected in rivers followed by artificial ponds, streams, well waters, and spring waters. Seaweeds contained a higher percentage of Arcobacter than the corresponding seawater samples. The isolates were identified as Arcobacter butzleri (96.4%) and Arcobacter cryaerophilus (3.6%). All the isolates showed a multi-drug resistance profile, being resistant to at least three different classes of antibiotics. Molecular analysis of genetic determinants responsible for tetracycline resistance in nine randomly chosen isolates revealed the presence of tetO and/or tetW. This work confirms the occurrence and the continuous emergence of antibiotic-resistant Arcobacter strains in environmental samples; also, the presence of quinolone-resistant Arcobacter spp. in aquatic sources used for water supply and irrigation represents a potential risk for human health.

scholarly journals Diversity and Antimicrobial Resistance in the Streptococcus bovis/Streptococcus equinus Complex (SBSEC) Isolated from Korean Domestic Ruminants

2021 ◽  
Vol 9 (1) ◽  
pp. 98
Author(s):  
Seon Young Park ◽  
Mingyung Lee ◽  
Se Ra Lim ◽  
Hyemin Kwon ◽  
Ye Seul Lee ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Phylogenetic Analyses ◽  
Tetracycline Resistance ◽  
Current Status ◽  
Streptococcus Bovis ◽  
Limited Information ◽  
Phenotypic Resistance ◽  
Domestic Ruminants ◽  
Antimicrobial Resistance Genes ◽  
Acid Producing Bacteria

S. bovis/S. equinus complex (SBSEC) includes lactic acid-producing bacteria considered as the causative agent associated with acute rumen lactic acidosis in intensive ruminants. Considering the limited information on the detailed characteristics and diversity of SBSEC in Korea and the emergence of antimicrobial resistance (AMR), we investigated the diversity of SBSEC from domestic ruminants and verified the presence of antimicrobial resistance genes (ARGs) against several antimicrobials with their phenotypic resistance. Among 51 SBSEC isolates collected, two SBSEC members (S. equinus and S. lutetiensis) were identified; sodA-based phylogenetic analyses and comparisons of overall genome relatedness revealed potential plasticity and diversity. The AMR rates of these SBSEC against erythromycin, clindamycin, and tetracycline were relatively lower than those of other SBSEC isolates of a clinical origin. An investigation of the ARGs against those antimicrobials indicated that tetracycline resistance of SBSECs generally correlated with the presence of tet(M)-possessing Tn916-like transposon. However, no correlation between the presence of ARGs and phenotypic resistance to erythromycin and clindamycin was observed. Although a limited number of animals and their SBSEC isolates were examined, this study provides insights into the potential intraspecies biodiversity of ruminant-origin SBSEC and the current status on antimicrobial resistance of the bacteria in the Korean livestock industry.

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