MANGROVE ASSOCIATE BASED SHRIMP FEED: AN INNOVATION IN THE AQUACULTURE SECTOR

The traditional method of shrimp culture is a common practice in Indian Sundarbans which is done without any sound scientific back-up, proper feed, and water quality management. The shrimp farmers use a traditional feed of animal origin that often results in deterioration of water quality and disease outbreaks in cultured species. The present paper highlights the effect of the total replacement of animal ingredients in shrimp feed with floral ingredients on water quality and shrimp health. Weight gain, condition index, feed conversion ratio (FCR), survival, body pigmentation (astaxanthin level) were analyzed in shrimps along with pond water quality. Higher condition index (C.I.) values, survival rate, and gain in shrimp weight were observed in the experimental pond (E) compared to the control pond (C). Low FCR values were observed in the experimental pond than in the control pond. Astaxanthin values in shrimps of the experimental pond were also higher than the control pond which points towards Suaeda maritima as the source of carotenoid in the shrimp tissue.

Cultivation of young-of-the-year carp with the use of chlorella (Chlorella (Beijerinck, 1890)) suspension

Purpose. Investigate ecological conditions and determine fish productivity of ponds when growing young-of-the-year carp using a suspension of chlorella. Methodology. During the study, we used methods generally accepted in hydrochemistry, hydrobiology and fish farming. Findings. The article presents the results of the study on the use of chlorella suspension in the cultivation of carp. It was found that the use of chlorella suspension created favorable hydrochemical conditions resulting in intensive development of zooplankton, which had a positive effect on the growth of young-of-the-year carp and fish productivity of the pond. The development of the natural food supply was sufficient to meet the nutritional needs of juvenile carp. The average seasonal biomass of zooplankton in the experimental pond was 28.68 g/m3 that was 1.5 times higher than in the control. The share of cladocerans in the total biomass of zooplankton in the experimental pond was 76.6%, versus 36.8% in the control. The average biomass of zoobenthos for the growing season in the experimental was 1.3 times higher than in the control, and was formed by chironomid larvae, which are valuable in the food chain. Originality. The study investigated ecological conditions and fish productivity of growing ponds under conditions of the application of cattle manure and suspension of chlorella when growing carp in monoculture. Practical value. It was found that the use of chlorella suspension promotes the intensification of the development of zooplankton organisms in ponds. The obtained data can be used to develop practical recommendations for optimizing the conditions for growing carp seeds and increasing the biological productivity of ponds. Key words: nursery ponds, natural food supply, phytoplankton, zooplankton, zoobenthos, young-of-the-year carp, chlorella suspension, fish productivity.

Consistent effects of pesticides on community structure and ecosystem function in freshwater systems

Predicting ecological effects of contaminants remains challenging because of the sheer number of chemicals and their ambiguous role in biodiversity-ecosystem function relationships. We evaluate responses of experimental pond ecosystems to standardized concentrations of 12 pesticides, nested in four pesticide classes and two pesticide types. We show consistent effects of herbicides and insecticides on ecosystem function, and slightly less consistent effects on community composition. Effects of pesticides on ecosystem function are mediated by alterations in the abundance and community composition of functional groups. Through bottom-up effects, herbicides reduce respiration and primary productivity by decreasing the abundance of phytoplankton. The effects of insecticides on respiration and primary productivity of phytoplankton are driven by top-down effects on zooplankton composition and abundance, but not richness. By demonstrating consistent effects of pesticides on communities and ecosystem functions and linking pesticide-induced changes in functional groups of organisms to ecosystem functions, the study suggests that ecological risk assessment of registered chemicals could be simplified to synthetic chemical classes or types and groups of organisms with similar functions and chemical toxicities.

Compound effect of nitrates and biotic factors on the in vitro activity of epithelial cilia of outer demibranchs of Sinanodonta woodiana (Mollusca, Bivalvia, Unionidae)

Effects of MPC, 2 MPC, and 3MPC water solutions of potassium nitrate and biotic factors (trematode infection and “gill pregnancy”) were studied on the duration of locomotion and beat frequency of epithelial cilia of outer demibranchs of Sinanodonta woodiаna (Lea, 1834). The species has recently invaded the system of water bodies of Ukraine. It has occupied numerous water bodies in the Kiliya distributary of Danube Delta, establishing populations of density and biomass. Statistically significant differences by the studied parameters of ciliary epithelium (duration of locomotion and beat frequency of cilia) were not observed in the control group of non-infected males and latent females of S. woodiana. In females with “gill pregnancy”, the duration of ciliary locomotion decreased by 1.5 times, and beat frequency by 1.4 times (P>99.9 %). It should be noted that the outer surface of demibranchs of female mollusks was completely covered by the filled to the brim marsupial pockets. Thus, the functional load of gills is high, leading to the development of compensatory processes in experimental animals. The processes induce increased effectiveness of protective factors that counteract them. Another biotic factor negatively affecting the experimental pond mussels was the high rates of trematode infection in the hosts’ reproductive glands which contained sporocysts and cercariae of Rhipidocotyle campanula Dujardin, 1845. Prevalence of that infection ranged from 50 to 100 %. These rates of infection were associated with sharply declining functions of cilia of the gill epithelium in studied animals. The compound pathogenic influence of parasite and “gill pregnancy” factors even more affected the functional parameters of outer gill ciliary epithelium. The MPC levels of toxicant in the water medium were accompanied with the first symptom of intoxication of the Chinese pond mussel, the stage of stimulation. At that point, the functional parameters of epithelial cilia of “pregnant” females and in moderately and weakly infected mollusks of both sexes increased in response to exposure to the toxic factor. With the growing concentrations of toxicant to 2 MPC and 3 MPC, the experimental pond mussels developed the pathological process of intoxication, which progressed from the stage of depression to sublethal and lethal stages. Accordingly, the locomotion activity of ciliary epithelium increasingly declined and, in the end, stopped. The “gill pregnancy” and high rates of trematode infection exacerbated the consequences of the process.

The results of cultivation and changes in fish and biological indicators of this year carp when using feed additives NuPro® та Bio-Mos

The intensification and development potential of the aquaculture sector has created challenges in finding alternative sources of protein for use in fish feed diets to develop feeds that provide adequate nutrition for fish growth while reducing the use of traditional protein sources. Therefore, the paper aimed to study the feasibility and effectiveness of feed additives “Bio-Mos” and “NuPro®” when implementing them into the diet of this year's carp (Сyprinus carpio). Researches were carried out in the conditions of the fishery “Rudnyky” of the Mykolaiv district of the Lviv region. The object of the study was this year's carp (Cyprinus carpio L.), which were grown in monoculture with artificial feed. In our research, it was found that the maximum average monthly temperature of the water supply source is set in August – +25.6 °С. The oxygen concentration was in the range of 6.5–7.3 mg/dm3. The highest values of permanganate oxidation were found in August – 12.8 g O2/m3, NO2 content increased slightly in July (0.052 mgN/l) and August (0.071 mgN/l) the number of nitrates increased slightly in August and September at 0.360 mgN/l and 0.281 mgN/l. The concentration of NH4+ was at the level of minimum values – 0.151–0.331 mg N/dm3. The phosphate content (PO4-3) fluctuated slightly in the range of 0.11–0.18 mg P/dm3. As a result of research, it was found that when the drug “NuPro” was included in the diet, the average individual weight of this year's carp was higher during the caught period by 24.3 %, and when using the additive “Bio-Mos” this figure exceeded the control by 28.6 %. Accordingly, the total mass of caught young carp from the second experimental pond exceeded this figure in the first control pond by 45.0 %, and in the third experimental pond, respectively – by 57.4 %. At the same time, “NuPro” та “Bio-Mos” increase the survival of young people from 9.3 % to 16.7 %. Therefore, when using feed additives “NuPro” and “Bio-Mos” in the amount of 2 % by weight of feed has a positive effect on the productivity of this year`s carp (Cyprinus carpio L.), which are grown in the III zone of fish farming.

Manganese (Mn) and Lead (Pb) Content in Lemna Culture Fertilized by Bioslurry

Lemna minor is a small water plant that floats on water and has the potential to be fresh feed for fish. Bioslurry is a by-product of the management of biogas from livestock manure which is often used as fertilizer for plants because it contains nutrients in a readily available form. Among the contents contained in bioslurry, manganese and lead are important things related to their use for plants and fish. This study aims to determine the mineral (Mn) and lead (Pb) content in the medium of lemna cultivation which is fertilized with various doses of bioslurry. The study was conducted at the Ciparanje Experimental Pond, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Indonesia. This study used an experimental method with Completely Randomized Design (CRD) with four treatments with three replications. The treatment in the form of adding bioslurry to the lemna culture media was 0.00%, 0.25%, 1.00% and 2.50% of the volume of water. The minor density of Lemna used is 800 g/m2. The culture was carried out in 4 cycles for 7 days. The parameters observed were Mn and Pb content in the lemna culture medium shortly after the addition of Bioslurry and after 7 days of lemna cultivation. Based on the results of the study, the use of 2.5% bioslurry concentration can produce the highest Mn content with a range of 2.0-4.6 ppm and 2.92 ppm Pb content after 7 days of lemna cultivation. In addition, the use of 2.5% bioslurry concentration resulted in Mn and Pb values in the culture medium shortly after stirring bioslurry in the lemna culture media. Based on the research results, the treatment of adding 2.5% bioslurry to the lemna culture media is the best dose for produced Mn for fish, as well as the safest Pb for aquaculture activities.

KONDISI PLANKTON PADA TAMBAK UDANG WINDU (Penaeus monodon FABRICIUS) DENGAN SUBSTRAT BERBEDA

Plankton is one of water microorganisms that plays an important role as natural food for fish and shrimp and as an stability indicator of water or aquatic environment.The purpose of this study was to determine the composition and abundance of plankton in tiger shrimp ponds using concrete ponds without substrate, concrete ponds using sand substrate and ponds. Sampling of plankton and water quality was carried out from March to July 2016 at PunagaTakalar Experimental Pond at 6 ponds consisted of concrete ponds without 2 subplot of sand (pond A) and concrete pond with 2 subplate sand subplate (pond B) respectively measuring 1,000 m2 and a 2-square-meter plot of 2,500–3,000 m2 (pond C). The results showed that plankton abundance in pond A ranged from 37–349 individuals/L, pond B ranged from 35–1,399 individuals/L and pond C ranged from 54–999 individuals/L. The most common phytoplankton genera in this study was Oscillatoria while for zooplankton is of Acartia. The diversity of plankton in ponds A, B and C is included in the community of unstable to moderate biota. In general, the diversity of plankton in pond B is included in the community of moderate biota. The plankton uniformity index in pond A, pond B and pond C is generally close to 1 this indicates that the existence of plankton species in the three ponds is relatively even.

Effects of granular and liquid fertilizers on phytoplankton under experimental pond condition

Under the experimental pond condition, three treatments of fertilizers on phytoplankton were carried out. The first treatment was with granular fertilizers (GF), and the second and third treatments were with liquid fertilizer A (LFA), low concentration and liquid fertilizer B (LFB), high concentration, respectively. The mean pH and the average transparency of the water varied significantly among different treatments (p < 0.05). Gross and net productivity in the LFB-treated ponds were higher than those observed in the two other treatments (p < 0.05). In addition, the bacillariophytes did not change significantly among treatments (p < 0.05). However, the chlorophytes increased in the LFB treatment, and the cyanophytes were increased in the GF treatment (p < 0.05). DOI: http://dx.doi.org/10.3329/bjb.v43i2.21670 Bangladesh J. Bot. 43(2): 175-181, 2014 (September)