The toxicity and toxicokinetics of imidacloprid and a bioactive metabolite to two aquatic arthropod species

AbstractSorghum panicles offer a very rich microenvironment for many insect pest species and their natural enemies. Thirty arthropod species belonging to 28 families, pertaining to 9 orders were obtained from sorghum panicles planted in Sohag Governorate, Egypt, during the 3 successive seasons of 2016–2018. Out of these species were 14 pests, 16 predators, and 3 parasitoids. Lepidopteran and hemipteran pests were the most dominant species-infested sorghum-panicles during the mature stages of the panicles. Three microlepidopteran pests, the noctuid, Eublemma (Autoba) gayneri (Roth.); the pyralid, Cryptoblabes gnidiella Millière, and the cosmopterigid, Pyroderces simplex Walsingham, were recorded as major pest species infesting sorghum panicles in Sohag Governorate. The dipteran parasitoid species, Nemorilla floralis (Fallen) (Tachinidae) emerged from the pupae of the E. gayneri and C. gnidiella, while the hymenopteran parasitoid, Brachymeria aegyptiaca (Chalcididae) was obtained from the pupae of all the studied microlepidopteran pests. Spiders, coccinellids, and Orius spp. were the dominant predators collected form panicles. Post-harvest, larvae, and pupae of lepidopteran pests, especially P. simplex recorded (147, 96, and 79 larvae) and (47, 30, and 73 pupae)/10 panicles in 2016, 2017, and 2018 seasons, respectively.

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Need for growing non-Bt cotton refugia to overcome Bt resistance problem in targeted larvae of the cotton bollworms, Helicoverpa armigera and Pectinophora gossypiella

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-021-00384-8 ◽

2021 ◽

Vol 31 (1) ◽

Author(s):

Muhammad Rafiq Shahid ◽

Muhammad Farooq ◽

Muhammad Shakeel ◽

Misbah Ashraf ◽

Zia Ullah Zia ◽

...

Keyword(s):

Helicoverpa Armigera ◽

Larval Mortality ◽

Pectinophora Gossypiella ◽

Bt Cotton ◽

Insect Larvae ◽

Plant Parts ◽

Arthropod Species ◽

Bt Resistance ◽

American Bollworm ◽

Helicoverpa Armígera

Abstract Background The effectiveness of Bacillus thuringiensis (Bt) cotton against target arthropod larvae is decreasing day by day. The comparative effect of Bt expression among Bt cotton varieties and different plant parts was observed against the cotton bollworms: Helicoverpa armigera and Pectinophora gossypiella larvae. Results In the present study, larval mortality of H. armigera was higher than P. gossypiella among selected Bt cultivars. Median lethal concentration (LC50) values were 8.91, 13.4, 14.0, and 36.4 for P. gossypiella, while 5.91, 4.04, 2.37, and 8.26 for H. armigera of FH-142, MNH-886, IR-3701, and FH-Lalazar, respectively. These values depicted that P. gossypiella had more Bt resistance problem than H. armigera larvae. The host range of both targeted insect larvae was different from each other due to the polyphagous feeding nature of the larvae of H. armigera that feed on different host plants, but P. gossypiella attacked only cotton with monophagous feeding habit. It was also notable from results that Bt expression in reproductive parts where the attacked pink bollworm was lower than the American bollworm, so the former had the maximum chance of resistance due to repeated exposure to Bt. Conclusions It was concluded that farmers be advised to follow the practice of growing non-Bt as a refuge crop to reduce the problem of Bt resistance in the target arthropod species.

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Response of Bioactive Metabolite and Biosynthesis Related Genes to Methyl Jasmonate Elicitation in Codonopsis pilosula

Molecules ◽

10.3390/molecules24030533 ◽

2019 ◽

Vol 24 (3) ◽

pp. 533 ◽

Cited By ~ 8

Author(s):

Jiao-jiao Ji ◽

Qi Feng ◽

Hai-feng Sun ◽

Xue-jun Zhang ◽

Xiao-xiao Li ◽

...

Keyword(s):

Methyl Jasmonate ◽

Adventitious Roots ◽

Multiple Shoots ◽

Bioactive Metabolites ◽

Bioactive Metabolite ◽

Codonopsis Pilosula ◽

Meja Treatment ◽

Metabolite Accumulation ◽

Culture Growth ◽

Sucrose Level

Bioactive metabolites in Codonopsis pilosula are of particular interest as an immunostimulant. Methyl jasmonate (MeJA) plays an important role in the elicitation of metabolite biosynthesis. Here, we explored the response of metabolites to MeJA elicitation in C. pilosula adventitious roots and multiple shoots. The results showed that the biomass, polysaccharide, and lobetyolin content of adventitious roots exhibited the highest increases with 100 µmol·L−1 MeJA at the 16th day of subculture, whereas the atractylenolide III (a terpenoid) content increased extremely with 50 µmol·L−1 MeJA treatment at the 7th day of subculture. In addition, the biomass and lobetyolin content significantly increased at the 4th day after treatment. Similarly, the polysaccharide and lobetyolin content increased in multiple shoots. Further identification of different metabolites responding to MeJA by 1H-NMR showed an extremely significant increase of the lobetyolinin level, which coincided with lobetyolin. Accordingly, the precursor, fatty acids, showed a highly significant decrease in their levels. Furthermore, a significant increase in β-d-fructose-butanol glycoside was detected, which was accompanied by a decrease in the sucrose level. Accordingly, the enzyme genes responsible for terpenoid and carbohydrate biosynthesis, CpUGPase, and CpPMK, were up regulated. In conclusion, MeJA promoted culture growth and accelerated bioactive metabolite accumulation by regulating the expression of the metabolite biosynthesis related genes, CpUGPase and CpPMK in C. pilosula.

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A Review of Antiviral and Antioxidant Activity of Bioactive Metabolite of Macroalgae within an Optimized Extraction Method

Energies ◽

10.3390/en14113092 ◽

2021 ◽

Vol 14 (11) ◽

pp. 3092

Author(s):

Rasha El-Shafei ◽

Hala Hegazy ◽

Bishnu Acharya

Keyword(s):

Antioxidant Activity ◽

Herpes Simplex Virus ◽

Herpes Simplex ◽

Extraction Method ◽

Bioactive Metabolites ◽

Active Metabolites ◽

Bioactive Metabolite ◽

Conventional Extraction ◽

Simplex Virus ◽

Macro Algae

Non-conventional extraction of bioactive metabolites could provide sustainable alternative techniques to preserve the potency of antioxidants and antiviral compounds extracted from macro-algae. In this paper, we first reviewed the antioxidant and antiviral potential of the active metabolites that exist in the three known macro-algae classes; Phaeophyceae, Rhodophyceae, and Chlorophyceae, and a comparison between their activities is discussed. Secondly, a review of conventional and non-conventional extraction methods is undertaken. The review then focused on identifying the optimal extraction method of sulphated polysaccharide from macro-algae that exhibits both antiviral and antioxidant activity. The review finds that species belonging to the Phaeophyceae and Rhodophceae classes are primarily potent against herpes simplex virus, followed by human immunodeficiency virus and influenza virus. At the same time, species belonging to Chlorophyceae class are recorded by most of the scholars to have antiviral activity against herpes simplex virus 1. Additionally, all three macro-algae classes exhibit antioxidant activity, the potency of which is a factor of the molecular structure of the bioactive metabolite as well as the extraction method applied.

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A Systematic Review of the Behavioral Responses by Stored-Product Arthropods to Individual or Blends of Microbially Produced Volatile Cues

Insects ◽

10.3390/insects12050391 ◽

2021 ◽

Vol 12 (5) ◽

pp. 391

Author(s):

Marco A. Ponce ◽

Tania N. Kim ◽

William R. Morrison III

Keyword(s):

Systematic Review ◽

Supply Chain ◽

Organic Compounds ◽

Behavioral Responses ◽

Mixed Effects ◽

Future Directions ◽

Arthropod Species ◽

Volatile Organic ◽

Volatile Cues ◽

Neutral Effect

Microbes are ubiquitous and play important ecological roles in a variety of habitats. While research has been largely focused on arthropods and microbes separately in the post-harvest supply chain, less attention has been paid to their interactions with each other. Up to this point, there has been no attempt to systematically describe the patterns of behavioral responses by stored-product insects to microbially produced volatile organic compounds (MVOCs). Thus, our aims were to evaluate whether stored-product arthropods were primarily and significantly attracted, repelled, or had a net neutral effect (e.g., unaffected or mixed) by MVOCs presented as (1) complex headspace blends or (2) single constituents and known mixtures. In total, we found 43 articles that contained 384 sets of tests with different combinations of methodology and/or qualitative findings, describing the behavioral responses of 24 stored-product arthropod species from two classes, four orders, and 14 families to 58 individual microbial compounds and the complex headspace blends from at least 78 microbial taxa. A total of five and four stored-product arthropod species were significantly attracted and repelled by MVOCs across odor sources, respectively, while 13 were unaffected or exhibited mixed effects. We summarize the biases in the literature, including that the majority of tests have occurred in the laboratory with a limited subset of methodology and has largely only assessed the preference of adult arthropods. Finally, we identify foundational hypotheses for the roles that MVOCs play for stored-product arthropods as well as gaps in research and future directions, while highlighting that the behavioral responses to MVOCs are complex, context-, and taxon-dependent, which warrants further investigation.

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Metabarcoding Malaise traps and soil eDNA reveals seasonal and local arthropod diversity shifts

Scientific Reports ◽

10.1038/s41598-021-89950-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ameli Kirse ◽

Sarah J. Bourlat ◽

Kathrin Langen ◽

Vera G. Fonseca

Keyword(s):

Habitat Preferences ◽

Life Cycles ◽

Soil Samples ◽

Turnover Rates ◽

Arthropod Diversity ◽

Arthropod Species ◽

Species Overlap ◽

Below Ground ◽

Phenological Shifts ◽

Malaise Traps

AbstractForest habitats host enormous diversity, but little is known about the seasonal turnover of arthropod species between the above- and below ground forest layers. In this study, we used metabarcoding approaches to uncover arthropod diversity in different forest types and seasons. Our study shows that metabarcoding soil eDNA and Malaise trap bulk samples can provide valuable insights into the phenology and life cycles of arthropods. We found major differences in arthropod species diversity between soil samples and Malaise traps, with only 11.8% species overlap. Higher diversity levels were found in Malaise traps in summer whereas soil samples showed a diversity peak in winter, highlighting the seasonal habitat preferences and life strategies of arthropods. We conclude that collecting time series of bulk arthropod samples and eDNA in the same locations provides a more complete picture of local arthropod diversity and turnover rates and may provide valuable information on climate induced phenological shifts for long-term monitoring.

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Arthropod community structure in pastures of an island archipelago (Azores): looking for local–regional species richness patterns at fine-scales

Bulletin of Entomological Research ◽

10.1079/ber2004289 ◽

2004 ◽

Vol 94 (2) ◽

pp. 111-121 ◽

Cited By ~ 22

Author(s):

P.A.V. Borges ◽

V.K. Brown

Keyword(s):

Species Richness ◽

Regional Scale ◽

Local Scale ◽

Local Species Richness ◽

Β Diversity ◽

Arthropod Species ◽

Α Diversity ◽

Local Species ◽

Richness Patterns

AbstractThe arthropod species richness of pastures in three Azorean islands was used to examine the relationship between local and regional species richness over two years. Two groups of arthropods, spiders and sucking insects, representing two functionally different but common groups of pasture invertebrates were investigated. The local–regional species richness relationship was assessed over relatively fine scales: quadrats (= local scale) and within pastures (= regional scale). Mean plot species richness was used as a measure of local species richness (= α diversity) and regional species richness was estimated at the pasture level (= γ diversity) with the ‘first-order-Jackknife’ estimator. Three related issues were addressed: (i) the role of estimated regional species richness and variables operating at the local scale (vegetation structure and diversity) in determining local species richness; (ii) quantification of the relative contributions of α and β diversity to regional diversity using additive partitioning; and (iii) the occurrence of consistent patterns in different years by analysing independently between-year data. Species assemblages of spiders were saturated at the local scale (similar local species richness and increasing β-diversity in richer regions) and were more dependent on vegetational structure than regional species richness. Sucking insect herbivores, by contrast, exhibited a linear relationship between local and regional species richness, consistent with the proportional sampling model. The patterns were consistent between years. These results imply that for spiders local processes are important, with assemblages in a particular patch being constrained by habitat structure. In contrast, for sucking insects, local processes may be insignificant in structuring communities.

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