Exploring the ammonium detoxification mechanism of young and mature leaves of the macrophyte Potamogeton lucens

2021 ◽  
pp. 105879
Author(s):  
Wyckliffe Ochieng ◽  
Ling Xian ◽  
Annah Timinah Nasimiyu ◽  
Samuel Wamburu Muthui ◽  
Leah Nyawira Ndirangu ◽  
...  
Agrotek ◽  
2018 ◽  
Vol 2 (3) ◽  
Author(s):  
Antonius Suparno ◽  
Opalina Logo ◽  
Dwiana Wasgito Purnomo

Sweet potato serves as a staple food for people in Jayawijaya. Many cultivars of sweet potatoes have been cultivated by Dani tribe in Kurulu as foot for their infant, child and adult as well as feeding especially for pigs. Base on the used of sweet potatoes as food source for infant and child, this study explored 10 different cultivars. As for the leaf morphology, it was indentified that the mature leaves have size around 15 � 18 cm. general outline of the leaf is reniform (40%), 60% have green colour leaf, 50% without leaf lobe, 60% of leaf lobes number is one, 70% of shape of central leaf lobe is toothed. Abazial leaf vein pigmentation have purple (40%), and petiole pigmentation is purple with green near leaf (60%), besides its tuber roots, sweet potatoes are also harvested for its shoots and green young leaves for vegetables.


2011 ◽  
Vol 16 (3) ◽  
pp. 236-241
Author(s):  
Chunmei Li ◽  
Young-Mee Lee ◽  
Kyeong-Cheol Lee ◽  
Woong Han ◽  
Myeong-Hyeon Wang ◽  
...  

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Dan Hou ◽  
Ling Li ◽  
Tengfei Ma ◽  
Jialong Pei ◽  
Zhongyu Zhao ◽  
...  

AbstractBamboo is known for its edible shoots and beautiful texture and has considerable economic and ornamental value. Unique among traditional flowering plants, many bamboo plants undergo extensive synchronized flowering followed by large-scale death, seriously affecting the productivity and application of bamboo forests. To date, the molecular mechanism of bamboo flowering characteristics has remained unknown. In this study, a SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1)-like gene, BoMADS50, was identified from Bambusa oldhamii. BoMADS50 was highly expressed in mature leaves and the floral primordium formation period during B. oldhamii flowering and overexpression of BoMADS50 caused early flowering in transgenic rice. Moreover, BoMADS50 could interact with APETALA1/FRUITFULL (AP1/FUL)-like proteins (BoMADS14-1/2, BoMADS15-1/2) in vivo, and the expression of BoMADS50 was significantly promoted by BoMADS14-1, further indicating a synergistic effect between BoMADS50 and BoAP1/FUL-like proteins in regulating B. oldhamii flowering. We also identified four additional transcripts of BoMADS50 (BoMADS50-1/2/3/4) with different nucleotide variations. Although the protein-CDS were polymorphic, they had flowering activation functions similar to those of BoMADS50. Yeast one-hybrid and transient expression assays subsequently showed that both BoMADS50 and BoMADS50-1 bind to the promoter fragment of itself and the SHORT VEGETATIVE PHASE (SVP)-like gene BoSVP, but only BoMADS50-1 can positively induce their transcription. Therefore, nucleotide variations likely endow BoMADS50-1 with strong regulatory activity. Thus, BoMADS50 and BoMADS50-1/2/3/4 are probably important positive flowering regulators in B. oldhamii. Moreover, the functional conservatism and specificity of BoMADS50 and BoMADS50-1 might be related to the synchronized and sporadic flowering characteristics of B. oldhamii.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shugang Zhao ◽  
Hongxia Wang ◽  
Kai Liu ◽  
Linqing Li ◽  
Jinbing Yang ◽  
...  

Abstract Background Tissue culture is an effective method for the rapid breeding of seedlings and improving production efficiency, but explant browning is a key limiting factor of walnut tissue culture. Specifically, the polymerization of PPO-derived quinones that cause explant browning of walnut is not well understood. This study investigated explants of ‘Zanmei’ walnut shoot apices cultured in agar (A) or vermiculite (V) media, and the survival percentage, changes in phenolic content, POD and PPO activity, and JrPPO expression in explants were studied to determine the role of PPO in the browning of walnut explants. Results The results showed that the V media greatly reduced the death rate of explants, and 89.9 and 38.7% of the explants cultured in V media and A media survived, respectively. Compared with that of explants at 0 h, the PPO of explants cultured in A was highly active throughout the culture, but activity in those cultured in V remained low. The phenolic level of explants cultured in A increased significantly at 72 h but subsequently declined, and the content in the explants cultured in V increased to a high level only at 144 h. The POD in explants cultured in V showed high activity that did not cause browning. Gene expression assays showed that the expression of JrPPO1 was downregulated in explants cultured in both A and V. However, the expression of JrPPO2 was upregulated in explants cultured in A throughout the culture and upregulated in V at 144 h. JrPPO expression analyses in different tissues showed that JrPPO1 was highly expressed in stems, young leaves, mature leaves, catkins, pistils, and hulls, and JrPPO2 was highly expressed in mature leaves and pistils. Moreover, browning assays showed that both explants in A and leaf tissue exhibited high JrPPO2 activity. Conclusion The rapid increase in phenolic content caused the browning and death of explants. V media delayed the rapid accumulation of phenolic compounds in walnut explants in the short term, which significantly decreased explants mortality. The results suggest that JrPPO2 plays a key role in the oxidation of phenols in explants after branch injury.


1972 ◽  
Vol 52 (4) ◽  
pp. 643-649 ◽  
Author(s):  
R. A. HAMLEN ◽  
F. L. LUKEZIC ◽  
J. R. BLOOM

Influence of clipping height on neutral carbohydrate levels in root exudates of alfalfa grown gnotobiotically was investigated by gas-chromatographic and mass-spectral techniques. Exudates were obtained from plants that were lightly clipped (removal of flower buds), intermediately clipped (15 cm), and severely clipped (removal of all but four mature leaves). Glucose, inositol, sucrose, and four unidentified (U) components were detected. Fluctuations in the level of sugars were observed in exudates from two sampling periods. Glucose and U1 were most concentrated under light clipping. Levels of inositol and sucrose were maximum under intermediate clipping. Amounts of U3 were greatest from severely clipped plants, whereas levels of U5 remained constant at all cuttings. Concentration of U4 was lowest under severe clipping. U2, present in exudates from seedling plants, was not detected. Quantities of sugars released per gram of dry weight of root tissue were greater under severe clipping. U1 was the major component, with glucose, inositol, and sucrose minor components of the total sugars.


2021 ◽  
Vol 22 (3) ◽  
pp. 1455
Author(s):  
Varsha Garg ◽  
Aleksandra Hackel ◽  
Christina Kühn

In potato plants, the phloem-mobile miR172 is involved in the sugar-dependent transmission of flower and tuber inducing signal transduction pathways and a clear link between solute transport and the induction of flowering and tuberization was demonstrated. The sucrose transporter StSUT4 seems to play an important role in the photoperiod-dependent triggering of both developmental processes, flowering and tuberization, and the phenotype of StSUT4-inhibited potato plants is reminiscent to miR172 overexpressing plants. The first aim of this study was the determination of the level of miR172 in sink and source leaves of StSUT4-silenced as well as StSUT4-overexpressing plants in comparison to Solanum tuberosum ssp. Andigena wild type plants. The second aim was to investigate the effect of sugars on the level of miRNA172 in whole cut leaves, as well as in whole in vitro plantlets that were supplemented with exogenous sugars. Experiments clearly show a sucrose-dependent induction of the level of mature miR172 in short time as well as long time experiments. A sucrose-dependent accumulation of miR172 was also measured in mature leaves of StSUT4-silenced plants where sucrose export is delayed and sucrose accumulates at the end of the light period.


Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1336
Author(s):  
Caterina Catalano ◽  
Loredana Abbate ◽  
Antonio Motisi ◽  
Dalila Crucitti ◽  
Vincenzo Cangelosi ◽  
...  

Polyploidy plays an important role in plant adaptation to biotic and abiotic stresses. Alterations of the ploidy in grapevine plants regenerated via somatic embryogenesis (SE) may provide a source of genetic variability useful for the improvement of agronomic characteristics of crops. In the grapevine, the SE induction process may cause ploidy changes without alterations in DNA profile. In the present research, tetraploid plants were observed for 9.3% of ‘Frappato’ grapevine somatic embryos regenerated in medium supplemented with the growth regulators β-naphthoxyacetic acid (10 µM) and N6-benzylaminopurine (4.4 µM). Autotetraploid plants regenerated via SE without detectable changes in the DNA profiles were transferred in field conditions to analyze the effect of polyploidization. Different ploidy levels induced several anatomical and morphological changes of the shoots and mature leaves. Alterations have been also observed in stomata. The length and width of stomata of tetraploid leaves were 39.9 and 18.6% higher than diploids, respectively. The chloroplast number per guard cell pair was higher (5.2%) in tetraploid leaves. On the contrary, the stomatal index was markedly decreased (12%) in tetraploid leaves. The observed morphological alterations might be useful traits for breeding of grapevine varieties in a changing environment.


Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1002
Author(s):  
María Gyomar Gonzalez-Gonzalez ◽  
Jose Blasco ◽  
Sergio Cubero ◽  
Patricia Chueca

Tetranychus urticae Koch is an important citrus pest that produces chlorotic spots on the leaves and scars on the fruit of affected trees. It is detected by visual inspection of the leaves. This work studies the potential of colour and hyperspectral imaging (400–1000 nm) under laboratory conditions as a fast and automatic method to detect the damage caused by this pest. The ability of a traditional vision system to differentiate this pest from others, such as Phyllocnistis citrella, and other leaf problems such as those caused by nutritional deficiencies, has been studied and compared with a more advanced hyperspectral system. To analyse the colour images, discriminant analysis has been used to classify the pixels as belonging to either a damaged or healthy leaves. In contrast, the hyperspectral images have been analysed using PLS DA. The rate of detection of the damage caused by T. urticae with colour images reached 92.5%, while leaves that did not present any damage were all correctly identified. Other problems such as damage by P. citrella were also correctly discriminated from T. urticae. Moreover, hyperspectral imaging allowed damage caused by T. urticae to be discriminated from healthy leaves and to distinguish between recent and mature leaves, which indicates whether it is a recent or an older infestation. Furthermore, good results were achieved in the discrimination between damage caused by T. urticae, P. citrella, and nutritional deficiencies.


2002 ◽  
Vol 15 (10) ◽  
pp. 1086-1094 ◽  
Author(s):  
Lawrence Lee ◽  
Peter Palukaitis ◽  
Stewart M. Gray

The requirement for the 17-kDa protein (P17) of Potato leafroll virus (PLRV) in virus movement was investigated in four plant species: potato (Solanum tuberosum), Physalis floridana, Nicotiana benthamiana, and N. clevelandii. Two PLRV P17 mutants were characterized, one that does not translate the P17 and another that expresses a P17 missing the first four amino acids. The P17 mutants were able to replicate and accumulate in agroinoculated leaves of potato and P. floridana, but they were unable to move into vascular tissues and initiate a systemic infection in these plants. In contrast, the P17 mutants were able to spread systemically from inoculated leaves in both Nicotiana spp., although the efficiency of infection was reduced relative to wild-type PLRV. Examination of virus distribution in N. benthamiana plants using tissue immunoblotting techniques revealed that the wild-type PLRV and P17 mutants followed a similar movement pathway out of the inoculated leaves. Virus first moved upward to the apical tissues and then downward. The P17 mutants, however, infected fewer phloem-associated cells, were slower than wild-type PLRV in moving out of the inoculated tissue and into apical tissues, and were unable to infect any mature leaves present on the plant at the time of inoculation.


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