scholarly journals The Effect of Insulin and Insulin-like Growth Factor 1 (IGF-1) on Cellular Proliferation and Migration of Human Subacromial Bursa Tissue

2021 ◽  
Author(s):  
Lukas N. Muench ◽  
Lisa Tamburini ◽  
Danielle Kriscenski ◽  
Arthur Landry ◽  
Daniel P. Berthold ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cellular Proliferation ◽  
Insulin Like Growth Factor ◽  
Subacromial Bursa ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals Human Cerebrospinal Fluid Regulates Proliferation and Migration of Stem Cells Through Insulin-Like Growth Factor-1

2015 ◽  
Vol 24 (2) ◽  
pp. 160-171 ◽  
Author(s):  
Mingxin Zhu ◽  
Yun Feng ◽  
Sean Dangelmajer ◽  
Hugo Guerrero-Cázares ◽  
Kaisorn L. Chaichana ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cerebrospinal Fluid ◽  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
Human Cerebrospinal Fluid ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals Up-Regulation of Insulin-Like Growth Factor-2 Promotes Cell Proliferation and Migration and is Associated with Bone Metastasis in Non Small Cell Lung Cancer.

2021 ◽  
Author(s):  
Shuai Han ◽  
Zhenhua Wang ◽  
Guangjian Bai ◽  
Zhitao Han ◽  
Yunpeng Bai ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Growth Factor ◽  
Bone Metastasis ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Insulin Like Growth Factor ◽  
Small Cell Lung ◽  
And Migration ◽  
Proliferation And Migration

Abstract Background:Non-small cell lung cancer (NSCLC) is a malignant tumor with high morbidity and mortality. About 40% NSCLC patients develop bone metastasis. The aberrant expression of IGF2 (insulin-like growth factor-2) is known to be linked with many malignancies, but it has not been reported in NSCLC. Methods and Results:We first used the GEO database for differential gene analysis and showed a correlation between IGF2 and bone metastasis in NSCLC. IGF2 was highly expressed in primary NSCLC and even more highly expressed in bone metastases. Our in vivo experiment also showed that IGF2 promoted lung cancer proliferation and migration. In addition, IGF2 overexpression activated AKT/NF-κB pathway by promoting IGF1R and INSR phosphorylation, and enhanced osteolytic lesions in lung cancer.Conclusions：Up-regulation of insulin-like growth factor-2 can promote the proliferation and migration of non-small cell lung cancer cells, and is related to bone metastasis.

scholarly journals The Influence of Curcumin on the Downregulation of MYC, Insulin and IGF-1 Receptors: A possible Mechanism Underlying the Anti-Growth and Anti-Migration in Chemoresistant Colorectal Cancer Cells

Medicina ◽  
2019 ◽  
Vol 55 (4) ◽  
pp. 90 ◽  
Author(s):  
Seyed Ahmad Hosseini ◽  
Hamid Zand ◽  
Makan Cheraghpour
Keyword(s):  
Colorectal Cancer ◽  
Growth Factor ◽  
Cancer Cells ◽  
Insulin Like Growth Factor ◽  
Anticancer Properties ◽  
Colorectal Cancer Cells ◽  
Cellular Homolog ◽  
And Migration ◽  
Migration Capacity ◽  
Proliferation And Migration

Background and objectives: Mounting evidence shows that curcumin, a bioactive substance originating from turmeric root, has anticancer properties. Additionally, curcumin prevents the migration and metastasis of tumor cells. However, the molecular mechanism involved in the anti-metastatic action of curcumin is not clear. Most studies have suggested that migration inhibition is related to curcumin’s anti-inflammatory properties. Curcumin possesses a regulatory effect on insulin and insulin-like growth factor-1 (IGF-1) receptors and signaling. Insulin signaling is one of the important pathways involved in tumor initiation and progression; therefore, we proposed that the anti-metastatic effect of curcumin may mediate the downregulation of insulin and insulin-like growth factor-1 receptors. Materials and Methods: Viable resistant cells resulting from treating SW480 cells with 5-fluorouracil (5-FU) were subjected to curcumin treatment to analyze the proliferation and migration capacity in comparison to the untreated counterparts. To test the proliferation and migration potential, MTT, colony formation, and wound healing assays were performed. Real-time polymerase chain reaction (RT-PCR) was performed to measure the mRNA expression of insulin-like growth factor-1R (IGF-1R), insulin receptor (IR), and avian myelocytomatosis virus oncogene cellular homolog (MYC). Results: Our findings showed that curcumin significantly decreased insulin and IGF-1 receptors in addition to MYC expression. Additionally, the downregulation of the insulin and insulin-like growth factor-1 receptors was correlated to a greater decrease in the proliferation and migration of chemoresistant colorectal cancer cells. Conclusions: These results suggest the possible therapeutic effectiveness of curcumin in adjuvant therapy in metastatic colorectal cancer.

Principles of wound healing and growth factor considerations

1993 ◽  
Vol 83 (4) ◽  
pp. 223-227 ◽  
Author(s):  
SJ Skokan ◽  
RH Davis
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Growth Factor ◽  
Wound Repair ◽  
Wound Closure ◽  
Cellular Proliferation ◽  
Positive Influence ◽  
Surgical Wounds ◽  
And Migration ◽  
Proliferation And Migration

This review examines some of the important principles in wound repair and significant considerations for the use of growth factors. Moisture provides a positive influence on the mechanical and hormonal aspects of wounds. Atraumatic closure of surgical wounds and postoperative care and the types of wound closure are discussed. Cellular proliferation and migration in wounds are central features regarding growth factors.

Platelet-Derived Exosomes Affect the Proliferation and Migration of Human Umbilical Vein Endothelial Cells Via miR-126

2019 ◽  
Vol 17 (4) ◽  
pp. 379-387 ◽  
Author(s):  
Yan Sun ◽  
Xiao-li Liu ◽  
Dai Zhang ◽  
Fang Liu ◽  
Yu-jing Cheng ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Real Time ◽  
Umbilical Vein ◽  
Angiogenic Factors ◽  
Healthy Donors ◽  
And Migration ◽  
Umbilical Vein Endothelial Cells ◽  
Tgf Β1 ◽  
Proliferation And Migration

Background:Intraplaque angiogenesis, the process of generating new blood vessels mediated by endothelial cells, contributes to plaque growth, intraplaque hemorrhage, and thromboembolic events. Platelet-derived Exosomes (PLT-EXOs) affect angiogenesis in multiple ways. The ability of miR-126, one of the best-characterized miRNAs that regulates angiogenesis, carried by PLT-EXOs to influence angiogenesis via the regulation of the proliferation and migration of endothelial cells is unknown. In this study, we aimed to investigate the effects of PLT-EXOs on angiogenesis by Human Umbilical Vein Endothelial Cells (HUVECs).Methods:We evaluated the levels of miR-126 and angiogenic factors in PLT-EXOs from Acute Coronary Syndrome (ACS) patients and healthy donors by real-time Polymerase Chain Reaction (PCR) and western blotting. We incubated HUVECs with PLT-EXOs and measured cell proliferation and migration with the Cell Counting Kit-8 assay and scratch assay, respectively. We also investigated the expression of miR-126 and angiogenic factors in HUVECs after exposure to PLT-EXOs by western blotting and real-time PCR.Results:PLT-EXOs from ACS patients contained higher levels of miR-126 and angiogenic factors, including Vascular Endothelial Growth Factor (VEGF), basic Fibroblast Growth Factor (bFGF), and Transforming Growth Factor Beta 1 (TGF-β1), than those from healthy donors (p<0.05). Moreover, the levels of exosomal miR-126 and angiogenic factors were increased after stimulation with thrombin (p<0.01). HUVEC proliferation and migration were promoted by treatment with activated PLT-EXOs (p<0.01); they were accompanied by the over-expression of miR-126 and angiogenic factors, including VEGF, bFGF, and TGF-β1 (p<0.01).Conclusion:Activated PLT-EXOs promoted the proliferation and migration of HUVECs, and the overexpression of miR-126 and angiogenic factors, thereby elucidating potential new therapeutic targets for intraplaque angiogenesis.

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