The Blood Biomarkers Associated With Unstable Atherosclerotic Plaques In Patients With Coronary Atherosclerosis

Atherosclerosis is a chronic long-lasting vascular disease leading to myocardial infarction and stroke. Vulnerable atherosclerotic (AS) plaques are responsible for these life-threatening clinical endpoints. To more successfully work against atherosclerosis, improvements in early diagnosis and treatment of AS plaque lesions are required. Vulnerable AS plaques are frequently undetectable by conventional imaging because they are non-stenotic. Although blood biomarkers like lipids, C-reactive protein, interleukin-6, troponins, and natriuretic peptides are in pathological ranges, these markers are insufficient in detecting the critical perpetuation of AS anteceding endpoints. Thus, chances to treat the patient in a preventive way are wasted. It is now time to solve this dilemma because clear results indicate a benefit of anti-inflammatory therapy per se without modification of blood lipids (CANTOS Trial, NCT01327846). This fact identifies modulation of immune-mediated inflammation as a new promising point of action for the eradication of fatal atherosclerotic endpoints.

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A Proteomic Study of Atherosclerotic Plaques in Men with Coronary Atherosclerosis

Diagnostics ◽

10.3390/diagnostics9040177 ◽

2019 ◽

Vol 9 (4) ◽

pp. 177 ◽

Cited By ~ 1

Author(s):

Ekaterina M. Stakhneva ◽

Irina A. Meshcheryakova ◽

Evgeny A. Demidov ◽

Konstantin V. Starostin ◽

Evgeny V. Sadovski ◽

...

Keyword(s):

Coronary Arteries ◽

Coronary Atherosclerosis ◽

Serum Amyloid ◽

Atherosclerotic Plaques ◽

Amyloid P Component ◽

Serum Amyloid P ◽

Serum Amyloid P Component ◽

Proteomic Study ◽

Amyloid P ◽

Β Chain

Background: To study the changes in protein composition of atherosclerotic plaques at different stages of their development in coronary atherosclerosis using proteomics. Methods: The object of research consisted of homogenates of atherosclerotic plaques from coronary arteries at different stages of development, obtained from 15 patients. Plaque proteins were separated by two-dimensional electrophoresis. The resultant protein spots were identified by the matrix-assisted laser desorption ionization method with peptide mass mapping. Results: Groups of differentially expressed proteins, in which the amounts of proteins differed more than twofold (p < 0.05), were identified in pools of homogenates of atherosclerotic plaques at three stages of development. The amounts of the following proteins were increased in stable atherosclerotic plaques at the stage of lipidosis and fibrosis: vimentin, tropomyosin β-chain, actin, keratin, tubulin β-chain, microfibril-associated glycoprotein 4, serum amyloid P-component, and annexin 5. In plaques at the stage of fibrosis and calcification, the amounts of mimecan and fibrinogen were increased. In unstable atherosclerotic plaque of the necrotic–dystrophic type, the amounts of human serum albumin, mimecan, fibrinogen, serum amyloid P-component and annexin were increased. Conclusion: This proteomic study identifies the proteins present in atherosclerotic plaques of coronary arteries by comparing their proteomes at three different stages of plaque development during coronary atherosclerosis.

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A Novel Biomarker of Coronary Atherosclerosis: Serum DKK1 Concentration Correlates with Coronary Artery Calcification and Atherosclerotic Plaques

Journal of Korean Medical Science ◽

10.3346/jkms.2011.26.9.1178 ◽

2011 ◽

Vol 26 (9) ◽

pp. 1178 ◽

Cited By ~ 30

Author(s):

Kwang-Il Kim ◽

Kyoung Un Park ◽

Eun Ju Chun ◽

Sang Il Choi ◽

Young-Seok Cho ◽

...

Keyword(s):

Coronary Artery ◽

Coronary Artery Calcification ◽

Coronary Atherosclerosis ◽

Atherosclerotic Plaques

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The Fatty Acids Composition In Patients With Coronary Atherosclerosis And Unstable Atherosclerotic Plaques

Atherosclerosis ◽

10.1016/j.atherosclerosis.2019.06.491 ◽

2019 ◽

Vol 287 ◽

pp. e163

Author(s):

V. Shramko ◽

Y. Ragino ◽

E. Kashtanova ◽

Y. Polonskaya ◽

S. Morozov ◽

...

Keyword(s):

Fatty Acids ◽

Coronary Atherosclerosis ◽

Atherosclerotic Plaques ◽

Fatty Acids Composition

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Research on the distribution spectrum of atherosclerotic plaques in patients with suspected coronary artery disease and the noninvasive screening model for coronary atherosclerosis burden

Quantitative Imaging in Medicine and Surgery ◽

10.21037/qims-20-901 ◽

2021 ◽

Vol 11 (7) ◽

pp. 3274-3285

Author(s):

Pengxi Han ◽

Jinyan Tang ◽

Ximing Wang ◽

Yuwen Su ◽

Guijie Li ◽

...

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Coronary Atherosclerosis ◽

Suspected Coronary Artery Disease ◽

Atherosclerotic Plaques ◽

Screening Model ◽

Artery Disease

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Analysis of differential expression of lipid metabolism genes in atherosclerotic plaques in patients with coronary atherosclerosis

The Siberian Journal of Clinical and Experimental Medicine ◽

10.29001/2073-8552-2021-36-4-156-163 ◽

2022 ◽

Vol 36 (4) ◽

pp. 156-163

Author(s):

E. V. Shakhtshneider ◽

D. E. Ivanoshchuk ◽

Yu. I. Ragino ◽

V. S. Fishman ◽

Ya. V. Polonskaya ◽

...

Keyword(s):

Lipid Metabolism ◽

Differential Expression ◽

Coronary Atherosclerosis ◽

Functional Class ◽

Atherosclerotic Plaques ◽

Old Patients ◽

Expression Levels ◽

Coronary Syndrome ◽

Different Types ◽

Lipid Metabolism Genes

Aim. The goal of the study was to analyze the differential expression of lipid metabolism-related genes in the atherosclerotic plaques of different types in patients with coronary atherosclerosis.Material and Methods. The study was performed on the specimens of atherosclerotic plaques in 45–65-year-old patients with coronary atherosclerosis with stable exertional angina functional class II-IV without acute coronary syndrome. Coronary atherosclerosis was verified by coronary angiography. Atherosclerotic plaque tissue was sampled intraoperatively when indicated. Whole-genome sequencing of ribonucleic acid (RNA) was performed using the TruSeq RNA Sample Preparation Kit (Illumina, USA).Results. We analyzed the differences in the expression of 12 genes including LDLR, APOB, PCSK9, LDLRAP1, LIPA, STAP1, ABCA1, APOA1, APOE, LPL, SCARB1, and SREBF2 depending on the type of atherosclerotic plaques. The expression level of APOE gene was eight times higher in unstable atherosclerotic plaques of dystrophic-necrotic type (p < 0.0001). The expression levels of LDLR and APOB genes were eight times higher in stable atherosclerotic plaques (p < 0.0001). We did not find differences in the expression levels of the ABCG5, ABCG8, APOC3, CETP, CLPS, CYP7A1, and PNPLA5 genes.Conclusion. The study showed the differences in the activity of individual metabolism-related genes in the atherosclerotic plaques of different types in patients with coronary atherosclerosis. Obtained data may become the basis for the development of test systems aimed at predicting the development of atherosclerotic process and its complications.

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Temperature Measurement of Advanced Murine Atherosclerotic Plaques

ASME 2007 Summer Bioengineering Conference ◽

10.1115/sbc2007-176307 ◽

2007 ◽

Author(s):

Frank Gijsen ◽

Anna Ten Have ◽

Jolanda Wentzel ◽

Antonius Van Der Steen

Keyword(s):

Heart Disease ◽

Vulnerable Plaque ◽

Acute Coronary Syndromes ◽

Coronary Atherosclerosis ◽

Developmental Stages ◽

Atherosclerotic Plaques ◽

Fibrous Cap ◽

Vulnerable Plaques ◽

Coronary Syndromes ◽

Plaque Detection

Ischaemic heart disease is most frequently caused by coronary atherosclerosis, of which the vulnerable plaque is one of the developmental stages. Rupture of a vulnerable plaque with superimposed thrombosis frequently leads to acute coronary syndromes. The major components of a vulnerable plaque are a lipid-rich, atheromatous core, and a thin fibrous cap with macrophage and macrophage infiltration (Schaar et al., 2004). After the first paper suggesting the possibility of thermographic detection of vulnerable plaques (Casscells et al., 1996), intracoronary thermography as a vulnerable plaque detection technique has been investigated. Increased metabolic activity of macrophages is suggested as the main reasons for the increased temperatures (ten Have et al., 2005).

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