Abstract
In previous publications on thiuram vulcanization we confined ourselves to the presentation and discussion of our extensive experimental work. We had investigated quantitatively the conversion of thiuram disulfides and were in a position to describe the kinetics of thiuram vulcanization. Such investigations are, of course, of particular value to technology for information about the rate and temperature dependence of vulcanization reactions. Our experiments were therefore not primarily designed to determine the details of reaction mechanism, which we had hitherto treated with restraint, and experience shows that the study of reaction kinetics does not always provide the key to a complete solution. All the same, it now seems reasonable to pick out some of the results we have obtained, and so consider the question of a reaction mechanism for thiuram vulcanization. We should, however, like to state, that although the present treatment concerns matters which may include some pertinent points, it does not necessarily represent a final interpretation. It is to be stressed, that any postulated reaction mechanism must explain what we regard as a stoichiometrical, i.e., concentration and temperature independent conversion of thiuram disulfide to zinc dithiocarbamate. The following experimental results thus formed the basis of our considerations:
A systematic analysis of acceptor specificity and reaction kinetics of five human α(2,3)sialyltransferases: Product inhibition studies illustrate reaction mechanism for ST3Gal-I
