Extracellular synthesis of nanoselenium from fresh water bacteria Bacillus sp., and its validation of antibacterial and cytotoxic potential

2020 ◽  
Vol 27 ◽  
pp. 101655
Author(s):  
S. Bharathi ◽  
S. Kumaran ◽  
G. Suresh ◽  
M. Ramesh ◽  
V. Thangamani ◽  
...  
Keyword(s):  
Fresh Water ◽  
Bacillus Sp ◽  
Cytotoxic Potential ◽  
Extracellular Synthesis

Cytotoxic Potential of Industrial Strains of Bacillus sp.

2002 ◽  
Vol 36 (2) ◽  
pp. 155-161 ◽  
Author(s):  
P.B. Pedersen ◽  
M.E. Bjørnvad ◽  
M.D. Rasmussen ◽  
J.N. Petersen
Keyword(s):  
Bacillus Sp ◽  
Cytotoxic Potential ◽  
Industrial Strains

scholarly journals Enhanced Biodegradation of Degreaser Using Pseudomonas and Bacillus Species in Fresh Water Ecosystem

2019 ◽  
pp. 1-10
Author(s):  
Renner Renner Nrior ◽  
Indutimi Mathias Otuogha
Keyword(s):  
Fresh Water ◽  
Heterotrophic Bacteria ◽  
Bacillus Species ◽  
Freshwater Ecosystem ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Microbiological Methods ◽  
Pseudomonas Species ◽  
Set Up ◽  
Hydrocarbon Utilizing Bacteria

Aim: The aim of this study is to enhance the biodegradation of degreasers used in upstream sectors of Nigeria Petroleum Industry using bio-augmenting organisms such as: Pseudomonas and Bacillus species in freshwater Ecosystem. Study Design: This study employs experimental designs, Randomized Block Design treatment set up, statistical analysis of data and interpretation. Place and Duration of Study: Freshwater sample for this research was collected from Asarama Andoni, in Rivers State, Nigeria. The study lasted for six months. Methodology: The experimental set-up was carried in 500 ml conical flask with two species of bacteria, two types of degreaser and fresh water sample giving a total of 8 set-up including controls. The Pseudomonas and Bacillus species used in this study were isolated from the freshwater ecosystem and identified using standard microbiological methods. The bioremediation potential of the respective test organisms were monitored at ambient temperature 28±0.2°C for 28 days at a constant interval of 7 days using the following Physiochemical parameter; Total dissolved Solid, Hydrogen concentrations ions and Total Hydrocarbon Content. While the following Microbiological parameters; Total heterotrophic Bacteria, Total Heterotrophic Fungi, Hydrocarbon Utilizing Bacteria, and Hydrocarbon Utilizing Fungi were monitored. Results: The percentage of degradability of the respective set-ups ranged from Control (Rigwash) (3.29%) < Pseudomonas sp. + Rigwash (27.56%) < Pseudomonas + Bacillus + Rigwash< (31.57%), Bacillus sp.+ Rigwash (37.57%) Control 2 (Aquabreak) (9.45%) < Pseudomonas sp.+ Aquabreak (26.77%) < Pseudomonas + Bacillus + Aquabreak (31.32%)< Bacillus sp.+ Aquabreak (32.46%). Overall evaluation revealed that Bacillus sp. had a higher biodegradation potential on both degreaser (Rigwash and Aquabreak) in freshwater than Pseudomonas sp. Five species of bacteria: Escherichia coli, Micrococcus, Citrobacter, Bacillus, and Pseudomonas species and four fungal species: Penicillium, Mucor, Aspergillus and Rhizopus species were isolated and identified as hydrocarbon utilizing bacteria and fungi organisms respectively. Conclusion: The results revealed that Bacillus species have more degradability potential than Pseudomonas species for both Aquabreak and Rigwash. These results also indicated the low biodegradation potential of Rigwash in fresh Ecosystem.

scholarly journals CYTOTOXIC POTENTIAL OF L-ASPARAGINASE FROM BACILLUS SP. IN VITRO

2019 ◽  
Vol 2 (2) ◽  
pp. 49 ◽  
Author(s):  
Renita Maria D'Souza ◽  
Asha Abraham
Keyword(s):  
Cell Line ◽  
Enzyme Concentration ◽  
Broth Culture ◽  
Phenotypic Characterization ◽  
Bacillus Sp ◽  
Cytotoxic Potential ◽  
3T3l1 Cell ◽  
Local Soil ◽  
Maximum Inhibition

This study reports the cytotoxic potential of L-Asparaginase isolated from Bacillus sp. Bacillus sp. was isolated from local soil/water samples and identified by rapid plate assay and further confirmed by phenotypic characterization. Extracellular L- Asparaginase was isolated from broth culture of Bacillus sp.  and purified by ammonium sulfate precipitation, followed by dialysis and ion exchange and gel filtration chromatography techniques. The purified enzyme was used to study the in vitro cytotoxic potential. Varying concentrations (31.25, 62.5, 125, 250, 500 µg/ml) of purified L-Asparaginase was tested on MCF7, HeLa, HepG2 and 3T3L1cell lines by MTT assay. Curcumin was maintained as a positive control. The results revealed that the enzyme showed a significant cytotoxic activity and a dose dependent effect. Minimum inhibition of (19.44%) was observed at an enzyme concentration of 31.25 µg/ml and maximum inhibition (71.14%) was observed at 500ug/ml against MCF7 cell line. Minimum inhibition of (10.04%) was observed at an enzyme concentration 31.25 µg/ml and maximum inhibition (68.92%) was observed at 500 µg/ml against HeLa cell line. Minimum inhibition of (7.45%) was shown at an enzyme concentration of 31.25 µg/ml and maximum inhibition (68.28 %) was observed at 500 µg/ml against HepG2 cell line. Minimum inhibition of (4.4%) was shown by enzyme concentration 31.25 µg/ml and maximum inhibition (47.4%) was observed at 500 µg/ml against 3T3L1 cell line. Curcumin at 5µM concentration showed an inhibition of 48.13% against MCF-7 cells, 54.42% against HeLa cells, 64.94% against HepG2 cells and 44.5% against 3T3L1 cell lines.

scholarly journals UJI IN VITRO BAKTERI ANTI QUORUM SENSING PENDEGRADASI ACYL HOMOSERINE LACTONE Aeromonas hydrophila

2017 ◽  
Vol 11 (3) ◽  
pp. 291
Author(s):  
Hessy Novita ◽  
Iman Rusmana ◽  
Munti Yuhana ◽  
Fachriyan Hasmi Pasaribu ◽  
Angela Mariana Lusiastuti
Keyword(s):  
Quorum Sensing ◽  
Fresh Water ◽  
Virulence Factors ◽  
Aeromonas Hydrophila ◽  
Homoserine Lactone ◽  
In Vitro Assay ◽  
Acyl Homoserine Lactone ◽  
Bacillus Sp ◽  
Vitro Assay

Anti quorum sensing (AQS) adalah proses inaktivasi atau degradasi molekul sinyal quorum sensing (QS) yaitu acyl homoserine lactone (AHL) tanpa memengaruhi pertumbuhan bakteri. Tujuan dari penelitian ini adalah untuk melakukan uji kultur bersama dan uji penghambatan faktor virulensi secara in vitro antara bakteri AQS dengan Aeromonas hydrophila sebagai patogen yang menyebabkan Motile Aeromonad Septicaemia (MAS) pada ikan air tawar. Hasil penelitian menunjukkan bahwa uji in vitro dengan kultur bersama antara bakteri AQS Bacillus sp. dan A. hydrophila tidak ada penghambatan pertumbuhan pada kedua bakteri, tetapi bakteri AQS dapat menghambat produksi faktor virulensi dari A. hydrophila yaitu protease dan hemolisin. AQS merupakan salah satu strategi yang potensial untuk diaplikasikan dalam pengendalian penyakit infeksius atau bakteri patogen resisten antibiotik pada budidaya ikan air tawar.Anti quorum sensing (AQS) was process of inactivation or degradation of Quorum sensing signal molecules of acyl homoserine lactone (AHL) without affecting growth of the bacteria. The aim of the reseach was to study in vitro assay of co-culture and inhibition of virulence factors between AQS bacteria which Aeromonas hydrophila as pathogen caused motile aeromonad septicaemia (MAS) in fresh water fish. The result showed that in vitro assay of co culture between AQS bacteria Bacillus sp. and A. hydrophila without inhibited of growth in both bacteria but bacteria AQS could suppressed production A. hydrophila virulence factors, protease, and hemolysin. The AQS is one of potential strategies to inhibit QS for application to control of infectious diseases or antibiotic resistant bacterial pathogens in fresh water aquaculture.

Cytotoxic potential of new phenylpropanoid derivatives isolated from Nectandra leucantha Nees & Mart (Lauraceae)

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
SS Grecco ◽  
EGA Martins ◽  
CR Figueiredo ◽  
N Girola ◽  
AL Matsuo ◽  
...  
Keyword(s):  
Cytotoxic Potential ◽  
Phenylpropanoid Derivatives

Aplikasi metode tahanan jenis dalam studi geologi karst Gua Seropan di Gunung Kidul, Yogyakarta

2017 ◽  
Vol 12 (2) ◽  
pp. 105-116
Author(s):  
Pulung A. Pranantya ◽  
Nurlia Sadikin
Keyword(s):  
Fresh Water ◽  
Ground Surface ◽  
Water Source ◽  
Raw Water ◽  
Limited Information ◽  
Resistivity Method ◽  
Central Java ◽  
Underground River ◽  
Initial Exploration

In terms of geology, most areas in south of the Gunungkidul District in Central Java consist of the Wonosari formation limestone. The land is generally very dry and source of raw water is also difficult to reach. Findings on the existence of underground river in caves, however, indicate the potential amount of water within the area, especially in the eastern part of the Gunungkidul District. Although limited information available, some fishermen have discovered that Seropan cave contains fresh water source. This cave is situated at 65 m below the cliff. Initial exploration, which done using a multichannel resistivity method, confirmed the availability of freshwater in the cave and underground river. The isopach of cave depth is found in ranges of 80 200 m below the ground surface. The water of Seropan cave can be utilized by implementing pipeline or by drilling at the suggested point based on the interpretation results, i.e. 110o2223.6388 EL 8o42.874 SL. [DY1][PP2][DY1]Perbaiki grammarIn terms of geology, most areas in south of Gunungkidul District in Central Java consist of the Wonosari formation limestone. The land is generally very dry and source of raw water is also difficult to reach. Findings on the exixtence of underground river in caves, however, indicate potential amount of water within the area especially in eastern part of Gunungkidul District. Although limited information available, some fishermans has discovered that Seropan cave contain fresh water source. This cave is situated at 65 m below the cliff. Initial exploration, which done using multichannel resistivity method, confirmed the availability of freshwater in the cave and underground river. The iso pach of cave depth is found in ranges of 80 200 m below the ground surface. The water of Seropan cave can be utilized by implementing pipeline or by drilling at the suggested point based on the interpretation results i.e. 110o2223.6388 EL 8o42.874 SL.[PP2]Sudah diperbaiki

STUDY OF POND STATUS IN INDIA : A REVIEW

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
V.K. YADAV ◽  
SONAM SHARMA ◽  
A.K. SRIVASTAVA ◽  
P.K. KHARE
Keyword(s):  
Fresh Water ◽  
Land Surface ◽  
Human Population ◽  
Climate Changes ◽  
Hydrological Cycle ◽  
Habitat Destruction ◽  
Conservation Strategy ◽  
Goods And Services ◽  
Human Use ◽  
Over Exploitation

Ponds are an important fresh water critical ecosystem for plants and animals providing goods and services including food, fodder, fish, irrigation, hydrological cycle, shelter, medicine, culture, aesthetic and recreation. Ponds cover less than 2 percent of worlds land surface. Ponds are important source of fresh water for human use. These are threatened by urbanization, industrialization, over exploitation, fragmentation, habitat destruction, pollution, illegal capturing of land and climate changes. These above factors have been destroying ponds very rapidly putting them in danger of extinction of a great number of local biodiversity. It is necessary to formulate a correct conservation strategy for pond restoration in order to meet the growing needs of fresh water by increasing the human population. Some measures have been compiled and proposed in the present review.

EVALUATION OF PTYCHOBOTHRIDEAN CESTODES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FROM FRESH WATER FISHES IN GODAVARI BASIN (M.S.) INDIA

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
SUNITA BORDE ◽  
ASAWARI FARTADE ◽  
AMOL THOSAR ◽  
RAHUL KHAWAL
Keyword(s):  
Fresh Water ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Band Pattern ◽  
Genomic Variation ◽  
Polymorphic Band ◽  
Rapd Profile ◽  
Band Size ◽  
Pcr Product ◽  
The Common

Ptychobothridean genera like Senga and Circumoncobothrium are the common parasites of fresh water fishes. The genotypic study of these parasites was taken by RAPD. The RAPD profile of these two parasites were not similar to each other as depicted by the band pattern in picture. These results suggest the presence of inter-specific polymorphism among cestode parasites of two different genera for RAPD analysis. The present study demonstrated that genetic differentiation of cestode parasites could be accomplished on the basis of genomic variation with polymorphic band pattern using RAPD. All the detected bands (PCR product) were polymorphic and band size ranged from 500-5000 bp in length. The RAPD of profiles using GBO-31, GBO-32, GBO-33, GBO-34, GBO-35 and GBO-36. Primers were able to characterize inter-specific polymorphism among the two genus ( Senga and Circumoncobothrium ). Genetic analysis suggests that Senga and Circumoncobothrium show genetic diversity with respect to RAPD patterns using all the six primers used for the present study. The genetic distance between the analyzed genuses ranged from 0.14 to 0.80. The differentiation of the two parasites on the basis of genetic markers could greatly facilitate study on the biology of these parasites.

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