Plasmodium berghei: dehydroepiandrosterone sulfate reverses chloroquino-resistance in experimental malaria infection; correlation with glucose 6-phosphate dehydrogenase and glutathione synthesis pathway

2004 ◽  
Vol 68 (10) ◽  
pp. 1903-1910 ◽  
Author(s):  
Innocent Safeukui ◽  
François Mangou ◽  
Denis Malvy ◽  
Philippe Vincendeau ◽  
Djavad Mossalayi ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Malaria Infection ◽  
Dehydroepiandrosterone Sulfate ◽  
Glutathione Synthesis ◽  
Glucose 6 Phosphate Dehydrogenase

scholarly journals Targeted Disruption of the Plasmodium berghei Ctrp Gene Reveals Its Essential Role in Malaria Infection of the Vector Mosquito

1999 ◽  
Vol 190 (11) ◽  
pp. 1711-1716 ◽  
Author(s):  
Masao Yuda ◽  
Hiroshi Sakaida ◽  
Yasuo Chinzei
Keyword(s):  
Plasmodium Berghei ◽  
Malaria Infection ◽  
Molecular Mechanisms ◽  
Mammalian Host ◽  
Insect Vector ◽  
Wild Type ◽  
Mosquito Midgut ◽  
Parasite Plasmodium ◽  
Vector Mosquito ◽  
Stage 1

CTRP (circumsporozoite protein and thrombospondin-related adhesive protein [TRAP]-related protein) of the rodent malaria parasite Plasmodium berghei (PbCTRP) makes up a protein family together with other apicomplexan proteins that are specifically expressed in the host-invasive stage 1. PbCTRP is produced in the mosquito-invasive, or ookinete, stage and is a protein candidate for a role in ookinete adhesion and invasion of the mosquito midgut epithelium. To demonstrate involvement of PbCTRP in the infection of the vector, we performed targeting disruption experiments with this gene. PbCTRP disruptants showed normal exflagellation rates and development into ookinetes. However, no oocyst formation was observed in the midgut after ingestion of these parasites, suggesting complete loss of their invasion ability. On the other hand, when ingested together with wild-type parasites, disruptants were able to infect mosquitoes, indicating that the PbCTRP gene of the wild-type parasite rescued infectivity of disruptants when they heterologously mated in the mosquito midgut lumen. Our results show that PbCTRP plays a crucial role in malaria infection of the mosquito midgut and suggest that similar molecular mechanisms are used by malaria parasites to invade cells in the insect vector and the mammalian host.

scholarly journals IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Ramatu Omenesa Bello ◽  
Maizaton Atmadini Abdullah ◽  
Roslaini Abd Majid ◽  
Voon Kin Chin ◽  
Mohammed Faruq Abd Rachman Isnadi ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Malaria Infection ◽  
Time Course ◽  
Systemic Circulation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Therapeutic Modalities ◽  
Barr Virus ◽  
Cytokine Activation ◽  
Human Disorders ◽  
Epstein Barr

Abstract Background The immune modulating potential of IL-35 in multiple human disorders has been reported. Consequent upon the recognition of inflammatory cytokine activation and its preponderance for mediating pathology during malaria infection, the study aimed to characterize the expression and functional contribution(s) of IL-35 in Plasmodium berghei (strain ANKA) infected mice. Methods Plasmodium berghei infection in male ICR mice was used as the rodent model of choice. The time course of IL-35 expression in the systemic circulation and tissues of P. berghei infected mice as well as their healthy control counterparts was assessed by enzyme linked immunosorbent assay and immunohistochemistry respectively. The effect of modulating IL-35 by recombinant IL-35 protein or neutralizing anti-Epstein-Barr virus-induced gene 3 antibody on the cytokine environment during P. berghei infection was assessed by flow cytometry. Furthermore, the influence of modulating IL-35 on histopathological hallmarks of malaria and disease progression was evaluated. Results Interleukin-35 was significantly up regulated in serum and tissues of P. berghei infected mice and correlated with parasitaemia. Neutralization of IL-35 significantly enhanced the release of IFN-γ, decreased the expression of IL-6 and decreased parasitaemia patency. Neutralization of IL-35 was also associated with a tendency towards increased survival as well as the absence of pathological features associated with malaria infection unlike recombinant IL-35 protein administration which sustained a normal course of infection and unfavourable malaria associated histological outcomes in P. berghei infected mice. Conclusion These results indicate the involvement of IL-35 in P. berghei induced malaria infection. IL-35 neutralization strategies may represent viable therapeutic modalities beneficial for the resolution of malaria infection.

scholarly journals INFLUENCE OF THE COMBINATION OF PASAK BUMI ROOT AND PROPOLIS EXTRACTS ON PARASITEMIA LEVELS IN MICE INFECTED WITH PLASMODIUM BERGHEI

2019 ◽  
pp. 268-271
Author(s):  
NUR HASANAH ◽  
HENDRI ASTUTY
Keyword(s):  
Combination Therapy ◽  
Plasmodium Berghei ◽  
Malaria Infection ◽  
Positive Control ◽  
Control Group ◽  
Control Groups ◽  
Propolis Extract ◽  
Blood Smears ◽  
Significant Difference ◽  
Global Concern

Objective: Malaria infection remains a global concern due to increasing resistance to artemisinin-based combination therapy. This study examinedthe antimalarial effects of propolis extract alone and in combination with pasak bumi root extract.Methods: In the study, 30 mice were divided into six groups including two control groups, two groups of mice treated with propolis aloneat concentrations of 90 and 180 mg/kg body weight (BW), and two combination groups of mice treated with 90 or 180 mg/kg BW propolis incombination with 60 or 75 mg/kg BW pasak bumi, respectively. Plasmodium berghei 2% was injected into each mouse, and blood smears wereprepared after 8 days to assess parasitemia.Results: The results revealed no significant difference in parasitemia levels between the positive control and the two combination groups (p=0.136 and0.289, respectively). However, superior growth inhibition (GI) results were observed in the combination groups (97.97% and 97.83%, respectively)than in the propolis monotherapy groups, whereas better outcomes were observed in the positive control group (98.63% GI) than in the propolismonotherapy groups (23.88% and 51.66%, respectively).Conclusion: These results illustrate that combination therapy is superior to propolis monotherapy in inhibiting parasitemia.

Exotoxin in malaria infection (Plasmodium berghei)

1967 ◽  
Vol 23 (1) ◽  
pp. 14-15 ◽  
Author(s):  
Chr Jerusalem ◽  
D. Bruchhausen
Keyword(s):  
Plasmodium Berghei ◽  
Malaria Infection

scholarly journals Synthetic peptide vaccine confers protection against murine malaria.

1987 ◽  
Vol 166 (5) ◽  
pp. 1591-1596 ◽  
Author(s):  
F Zavala ◽  
J P Tam ◽  
P J Barr ◽  
P J Romero ◽  
V Ley ◽  
...  
Keyword(s):  
Tetanus Toxoid ◽  
Plasmodium Berghei ◽  
Synthetic Peptide ◽  
Malaria Infection ◽  
Peptide Vaccine ◽  
High Serum ◽  
Circumsporozoite Protein ◽  
Synthetic Peptide Vaccine ◽  
Repeat Domain ◽  
A Subunit

A synthetic peptide, (DPPPPNPN)2D, representing a subunit of the repeat domain of the Plasmodium berghei circumsporozoite protein, was conjugated to tetanus toxoid using bisdiazobenzidine. Immunization of mice and rats with the conjugate induced high serum titers of antibodies to the parasite, and most of the animals were completely protected from malaria infection when challenged with sporozoites.

scholarly journals In Vivo Hemozoin Kinetics after Clearance of Plasmodium berghei Infection in Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Rosangela Frita ◽  
Daniel Carapau ◽  
Maria M. Mota ◽  
Thomas Hänscheid
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
Malaria Infection ◽  
Blood Stream ◽  
Biologically Active ◽  
Immune Functions ◽  
Parasite Replication ◽  
Kinetics Of

Hemozoin (Hz) is released into the blood stream after rupture of infected red blood cells (iRBCs) at the end of each parasite replication cycle. This free Hz is ingested by circulating and resident phagocytes. The presence of Hz in tissues after clearance of infection has been previously reported. Still, little is known about the kinetics of Hz in vivo, during and after Plasmodium infection. It is particularly important to understand Hz kinetics after malaria infections as it has been reported that Hz is associated with impairment of immune functions, including possible consequences for coinfections. Indeed, if Hz remains biologically active for prolonged periods of time inside immunocompetent cells, the potential consequences of such accumulation and presence to the immune system should be clarified. Here, using several independent methods to assess the presence of Hz, we report the long-term in vivo kinetics of Hz in diverse organs in a murine model of malaria infection.

scholarly journals Protection of renal function by four selected plant extracts during Plasmodium berghei infection

2015 ◽  
Vol 5 (10) ◽  
pp. 339 ◽  
Author(s):  
Adewale Adetutu ◽  
Olubukola Sinbad Olorunnisola ◽  
Kazeem Iyanda
Keyword(s):  
Renal Function ◽  
Body Weight ◽  
Blood Urea Nitrogen ◽  
Aqueous Extract ◽  
Plant Extracts ◽  
Plasmodium Berghei ◽  
Malaria Infection ◽  
Renal Tissue ◽  
Untreated Group ◽  
Urea Nitrogen

Background: Weakening of renal function from reactive oxygen species generated during malaria infection is one of the prominent causes of death in prevalent regions. The potential toxicity of free radical generated by malaria parasites are counteracted by a large number of cytoprotective phytochemicals. Therefore, this study examined the influence of extracts of five selected antimalarial plants (Azadirachta indica, Parquetina nigrescens, Citrus paradisi, and Khaya senigalensis) on reduction of inflammation in renal tissue, blood urea nitrogen and creatinine levels during malaria infection using Plasmodium berghei infected Swiss albino mice. For in vivo assay, mice were inoculated with 1 × 107 parasitized erythrocytes and plant extracts were subsequently administered orally at 100 mg/kg body weight once a day for 17 consecutive days. The chemo-suppressive and prophylaxis effects of the plant extracts against P. berghei were investigated and compared with those of standard antimalarial drug, chloroquine. Tail bleeding was performed to check the percentage parasitaemia by making a thin film smear on a slide, stained in Giemsa. The numbers of parasited cells against the unparasitised cells were counted using a microscope. The effect of malaria infection on renal tissue was assessed by histological analysis and measurement of blood urea nitrogen and creatinine levels in plasma. At 100 mg/kg per body weight, aqueous extract of K. senegalensis, A. indica, C. paradisi and P. nigrescens exhibited significant (p<0.05) percentage inhibition and chemo-suppressive effects in comparison with the chloroquine treated mice. The result of the untreated group showed that there was a significant (p<0.05) increase in the level of plasma urea while the level of the groups treated with plants extract stabilized the level of urea and creatinine in the blood. Also there was a pathological lesion on the kidney tissue of untreated group whereas the group treated with aqueous extract of A. indica, Khaya senegalensis and C. paradisi showed no lesion. It can be established that the extracts can protect and preserve renal function during malaria infection. These findings justified the use of the extracts in traditional medicine practice, for the treatment of malaria infection.  Keywords: Plasmodium berghei, antimalarial plants, renal function, antiplasmodium

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