A stilbene dimer and flavonoids from the aerial parts of Chromolaena odorata with proprotein convertase subtilisin/kexin type 9 expression inhibitory activity

One new flavonol diglycoside, 4′,5-dihydroxy-3,3′,7-trimethoxyflavone 4′- O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (1), and two known compounds (2–3) were isolated from the methanol extract of Anoectochilus annamensis Aver. aerial parts. The effects were evaluated of all isolated compounds (1–3) on LPS-induced production of the inflammatory mediator nitric oxide (NO) by RAW264.7 cells. 4′,5-Dihydroxy-3,3′,7-trimethoxyflavone (2) was the most active while the addition of a rutinoside at C-4′ (compound 1) decreased the inhibitory activity. This is the first report on the chemical composition and biological activity of A annamensis.

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GC-MS Investigation of Amaryllidaceae Alkaloids in Galanthus xvalentinei nothosubsp. subplicatus

Natural Product Communications ◽

10.1177/1934578x1300800312 ◽

2013 ◽

Vol 8 (3) ◽

pp. 1934578X1300800

Author(s):

Buket Bozkurt Sarikaya ◽

Strahil Berkov ◽

Jaume Bastida ◽

Gulen Irem Kaya ◽

Mustafa Ali Onur ◽

...

Keyword(s):

Inhibitory Activity ◽

Anticholinesterase Activity ◽

Amaryllidaceae Alkaloids ◽

Acetylcholinesterase Inhibitory Activity ◽

Aerial Parts ◽

Ms Analysis ◽

Ellman’S Method ◽

First Time

A GC-MS analysis of alkaloids in the aerial parts and bulbs of Galanthus x valentinei nothosubsp. subplicatus was performed for the first time. Totally, twenty-six alkaloids were identified, of which tazettine and galanthindole were the major ones. Acetylcholinesterase inhibitory activity of the alkaloidal extracts was determined using modified in vitro Ellman's method. Significant anticholinesterase activity was observed in the tested samples (bulbs: IC50 = 21.3 μg/mL, aerial parts: IC50 = 16.3 μg/mL).

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Acetylcholinesterase and Butyrylcholinesterase Inhibitory Compounds from Chelidonium Majus (Papaveraceae)

Natural Product Communications ◽

10.1177/1934578x1000501110 ◽

2010 ◽

Vol 5 (11) ◽

pp. 1934578X1000501 ◽

Cited By ~ 1

Author(s):

Lucie Cahlíková ◽

Lubomír Opletal ◽

Milan Kurfürst ◽

Kateřina Macáková ◽

Andrea Kulhánková ◽

...

Keyword(s):

Human Plasma ◽

Human Blood ◽

Inhibitory Activity ◽

Dependent Manner ◽

Isoquinoline Alkaloids ◽

Chelidonium Majus ◽

Aerial Parts ◽

Naturally Occurring ◽

Inhibitory Compounds ◽

Dose Dependent

The roots and aerial parts of Chelidonium majus L. were extracted with EtOH and fractionated using CHCl3 and EtOH. Repeated column chromatography, preparative TLC and crystallization led to the isolation of five isoquinoline alkaloids, stylopine (3), chelidonine (4), homochelidonine (5), protopine (6), and allocryptopine (7), along with two isolation artifacts 6-ethoxydihydrosanguinarine (1) and 6-ethoxydihydrochelerythrine (2). All isolated compounds were tested for human blood acetylcholinesterase (HuAChE) and human plasma butyrylcholinesterase (HuBuChE) inhibitory activity. The isolation artifacts exhibited the highest activity against HuAChE and HuBuChE with IC50 values of 0.83 ± 0.04 μM and 4.20 ± 0.19 μM for 6-ethoxydihydrochelerythrine and 3.25 ± 0.24 μM and 4.51 ± 0.31 μM for 6-ethoxydihydrosanguinarine. The most active of the naturally-occurring alkaloids was chelidonine, which inhibited both HuAChE and HuBuChE in a dose-dependent manner with IC50 values of 26.8 ± 1.2 μM and 31.9 ± 1.4 μM, respectively.

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LC-MS- and NMR-Guided Isolation of Monoterpene Dimers from Cultivated Thymus vulgaris Varico 3 Hybrid and Their Antityrosinase Activity

Planta Medica ◽

10.1055/a-0927-7041 ◽

2019 ◽

Vol 85 (11/12) ◽

pp. 941-946 ◽

Cited By ~ 1

Author(s):

Ilias Stefanis ◽

Dimitra Hadjipavlou-Litina ◽

Anna-Rita Bilia ◽

Anastasia Karioti

Keyword(s):

Ursolic Acid ◽

Oleanolic Acid ◽

Inhibitory Activity ◽

Kojic Acid ◽

2D Nmr ◽

Thymus Vulgaris ◽

Reference Compound ◽

Aerial Parts ◽

Spectroscopic Analyses ◽

Antityrosinase Activity

AbstractTargeted isolation based on a combination of NMR and HPLC-PDA-MS of a dichloromethane extract of Thymus vulgaris Varico 3 aerial parts afforded one new p-cymene dimer, 6,3′,4′-trihydroxy-5,5′-diisopropyl-2,2′-dimethylbiphenyl (1), together with two known p-cymene derivatives (2 and 3), as well as five known compounds, namely, thymol (4), oleanolic acid (5), ursolic acid (6), cirsimaritin (7), and xanthomicrol (8). The structural elucidation of all compounds was performed by spectroscopic analyses, including 1D and 2D NMR, and HRESIMS experiments. The biphenyls were assayed for their inhibitory activity on tyrosinase. Compounds 2 and 3 showed negligible activity on tyrosinase, while compound 1 effectively inhibited the enzyme with 35% (± 0.3) inhibitory activity, higher than the inhibition of the reference compound kojic acid (18.6 ± 0.02).

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GC-MS Investigation and Acetylcholinesterase Inhibitory Activity of Galanthus rizehensis

Zeitschrift für Naturforschung C ◽

10.1515/znc-2013-3-407 ◽

2013 ◽

Vol 68 (3-4) ◽

pp. 118-124 ◽

Cited By ~ 2

Author(s):

Buket Bozkurt Sarikaya ◽

Nehir Unver Somer ◽

Gulen Irem Kaya ◽

Mustafa Ali Onur ◽

Jaume Bastida ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Inhibitory Activity ◽

Gas Chromatography Mass Spectrometry ◽

Acetylcholinesterase Inhibitory Activity ◽

Aerial Parts ◽

Chromatography Mass Spectrometry ◽

Inhibitory Activities ◽

Alkaloid Extracts ◽

Potent Inhibitory Activity

GC-MS (gas chromatography-mass spectrometry) analyses of alkaloids in the aerial parts and bulbs of Galanthus rizehensis Stern (Amaryllidaceae), collected during two different vegetation periods, was performed. Twenty three alkaloids were identified in four different alkaloid extracts. Acetylcholinesterase (AChE) inhibitory activities of the alkaloid extracts were tested. Both the highest alkaloid diversity and the most potent inhibitory activity (IC50 12.94 μg/ml) were obtained in extracts from the bulbs of G. rizehensis collected during the fruiting period.

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New Humulenes from Hyptis incana (Labiatae)

Natural Product Communications ◽

10.1177/1934578x1300801201 ◽

2013 ◽

Vol 8 (12) ◽

pp. 1934578X1300801

Author(s):

Mitsuru Satoh ◽

Yoshio Satoh ◽

Yasuhiro Anzai ◽

Daisuke Ajisawa ◽

Keiichi Matsuzaki ◽

...

Keyword(s):

Cytotoxic Activity ◽

Inhibitory Activity ◽

Leukemia Cells ◽

Moderate Activity ◽

Mouse Leukemia ◽

Aerial Parts ◽

Growth Inhibitory ◽

Growth Inhibitory Activity ◽

Potent Growth

Two new humulene-type sesquiterpenes, named hyptishumulene I (1) and II (2), have been isolated, together with eight known compounds, a humulene-type sesquiterpene (3), a monoterpene (4) and six abietane-type diterpenoids (5–10) from the aerial parts of Hyptis incana (Labiatae). The cytotoxic activity of the isolated compounds against mouse leukemia cells (L1210) was examined. The abietane-type diterpenoids (5–10) showed rather potent growth inhibitory activity (IC50<15 μM), while the new humulene-type compounds (1 and 2) exhibited moderate activity (IC50>50 μM).

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Caffeoylquinic acid derivatives isolated from the aerial parts of Gynura divaricata and their yeast α-glucosidase and PTP1B inhibitory activity

Fitoterapia ◽

10.1016/j.fitote.2014.08.015 ◽

2014 ◽

Vol 99 ◽

pp. 1-6 ◽

Cited By ~ 64

Author(s):

Jian Chen ◽

Sven Mangelinckx ◽

Li Ma ◽

Zhengtao Wang ◽

Weilin Li ◽

...

Keyword(s):

Inhibitory Activity ◽

Caffeoylquinic Acid ◽

Aerial Parts

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ChemInform Abstract: Benzyl Glycosides from the Aerial Parts of Gynostemma laxum and Their NF-ϰB Inhibitory Activity in HepG2 Cells

ChemInform ◽

10.1002/chin.201209207 ◽

2012 ◽

Vol 43 (9) ◽

pp. no-no

Author(s):

Pham Thanh Ky ◽

Pham Tuan Anh ◽

Phan Van Kiem ◽

Chau Van Minh ◽

Nguyen Xuan Nhiem ◽

...

Keyword(s):

Inhibitory Activity ◽

Hepg2 Cells ◽

Aerial Parts

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Chemical Constituents From the Aerial Part of Tiliacora triandra (Colebr.) Diels and Their α-Glucosidase and α-Amylase Inhibitory Activity

Natural Product Communications ◽

10.1177/1934578x19899595 ◽

2020 ◽

Vol 15 (1) ◽

pp. 1934578X1989959

Author(s):

Emmanuel Ayobami Makinde ◽

Chitchamai Ovatlarnporn ◽

Chonlatid Sontimuang ◽

Gaëtan Herbette ◽

Opeyemi Joshua Olatunji

Keyword(s):

Inhibitory Activity ◽

Chemical Constituents ◽

Ethyl Linoleate ◽

Resonance Spectroscopy ◽

Ionization Mass ◽

Pheophorbide A ◽

Aerial Parts ◽

Fatty Acid Derivatives ◽

First Time

Two new fatty acid derivatives identified as 5,7-dihydroxy-6-oxoheptadecanoic acid (1) and ethyl-5,7-dihydroxy-6-oxooctadecanoate (2) together with four known compounds, ethyl linolenate (3), ethyl linoleate (4), ethyl pheophorbide A (5), and pheophorbide A (6), were isolated from the aerial parts of Tiliacora triandra. All the compounds were isolated from T. triandra for the first time. The structures of the compounds were elucidated using high-resolution electrospray ionization mass spectrometry, 1-dimensional and 2-dimensional nuclear magnetic resonance spectroscopy, and comparison with literature data. All the isolated compounds were evaluated for their in vitro inhibitory activity against α-glucosidase and α-amylase. Compounds 1-6 exhibited α-glucosidase inhibitory activity with half-maximal inhibitory concentration values (IC50) values in the range of 11.58-424.06 μM, while only compound 1 displayed inhibitory activity against α-amylase at an IC50 value of 26.27 μM.

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