Concentration-dependent suppressive effect of shrimp head protein hydrolysate on dehydration-induced denaturation of lizardfish myofibrils

2006 ◽  
Vol 97 (5) ◽  
pp. 762-769 ◽  
Author(s):  
Yaowalux Ruttanapornvareesakul ◽  
Misako Ikeda ◽  
Kenji Hara ◽  
Kiyoshi Osatomi ◽  
Kazufumi Osako ◽  
...  
2005 ◽  
Vol 11 (3) ◽  
pp. 261-268 ◽  
Author(s):  
Yaowalux RUTTANAPORNVAREESAKUL ◽  
Kenji HARA ◽  
Kiyoshi OSATOMI ◽  
Kazufumi OSAKO ◽  
Orawan KONGPUN ◽  
...  

2006 ◽  
Vol 72 (2) ◽  
pp. 421-428 ◽  
Author(s):  
Yaowalux RUTTANAPORNVAREESAKUL ◽  
Kingduean SOMJIT ◽  
Akinori OTSUKA ◽  
Kenji HARA ◽  
Kiyoshi OSATOMI ◽  
...  

2005 ◽  
Vol 11 (1) ◽  
pp. 49-54 ◽  
Author(s):  
M. Mizani ◽  
M. Aminlari ◽  
M. Khodabandeh

Enzymatic hydrolysis has been widely applied for production of protein hydrolysate from shrimp waste and for purification of chitin. In the present study, shrimp (P. semisulcatus) head waste was hydrolysed, using a commercial proteolytic enzyme, Alcalase. In order to improve protein extraction efficiency, certain chemicals such as sodium sulphite and Triton x-100 were used along with the enzyme. When Alcalase (12 AU/kg) used alone, the yield of protein extraction was 45.1% and by using Triton x-100 (0.01 g/kg) together with Alcalase, the yield was decreased to 39%, whereas the presence of sodium sulphite (200 mmol/L) with the enzyme or with the enzyme and Triton x-100 increased the level of protein extraction to 62% and 65.1%, respectively. The resulting protein powder contained sufficient amounts of essential amino acids to be used in feed formulations. By precipitating proteins from the resulting protein extract at pH 3.1, the residual sulphite in protein powder was decreased by 97% and thus the powder can be considered suitable for animal and/or aquaculture feed formulations.


2005 ◽  
Vol 71 (1) ◽  
pp. 220-228 ◽  
Author(s):  
Yaowalux RUTTANAPORNVAREESAKUL ◽  
Misako IKEDA ◽  
Kenji HARA ◽  
Kazufumi OSAKO ◽  
Orawan KONGPUN ◽  
...  

Author(s):  
Tam Dinh Le Vo Vo

The aim of this study is to obtain the copper-binding protein hydrolysate from the white leg shrimp head (WLSH) by-product using enzymatic hydrolysis. The outcome indicated that the copper-binding capacity (CBC) of the WLSH protein hydrolysate achieved the maximum value of 19.4±0.5 mg Cu2+/g protein with hydrolysis conditions including Flavourzyme preparation, pH 7.5, 50°C, the enzyme:substrate (E:S) ratio of 80 U/g protein and 5h of hydrolysis. Under the pH treatment in a range of 1-11 or heat treatment at 100°C for up to 180 min, its CBC remained over 80%. The water holding capacity (WHC) and the oil-holding capacity (OHC) of the protein hydrolysate were 4.1±0.1 ml water/g protein hydrolysate powder and 4.5±0.1 ml oil/g protein hydrolysate powder, respectively. The solution also encompassed up to 8 essential amino acids, accounting for 36.1% of the total amino acid content. The protein hydrolysate could serve not only as a copper chelating agent, preventing copper-deficient or superfluous relating diseases, but also as a texturizer and an amino acid supplement fortified in various types of food.


2021 ◽  
Vol 322 ◽  
pp. 04005
Author(s):  
Witya Hafifani ◽  
Indun D. Puspita ◽  
Masagus M. P. Putra

Swamp eel (Monopterus albus) processing produced byproducts such as heads that possess a high protein, carbohydrate, lipid, and energies content. Swamp eel heads were hydrolyzed (SEHPH) and tested for their antibacterial activity against histamine-producing bacteria (HPB) to explore the potency. The hydrolysis was conducted by a commercial papain enzyme PAYA (concentration 4%) with various hydrolysis times (0, 60, 120, and 180 minutes) at 60o C with pH 5. The results indicated that increasing hydrolysis time increased solubility and the degree of hydrolysis. The inhibitor concentration 50 test using microdilution of SEHPH inhibited 50% against three HPB named Citrobacter sp. (CK1), Klebsiella sp. (CK13), and Morganella morganii (TK7) at 20 mg/ml. The optimum antibacterial activity test by disk diffusion method was formed by each minute of hydrolysis with the best inhibition zone to Klebsiella sp. (CK13). The macrodillution method showed the highest inhibition was produced by 180 hydrolyses with 45% on CK1, 54% on CK13, and 48% on TK7. These results indicated that SEHPH has potency as an antibacterial agent to reduce histamine-producing bacteria.


2002 ◽  
Vol 82 (7) ◽  
pp. 753-759 ◽  
Author(s):  
Maribel Plascencia-Jatomea ◽  
Miguel A Olvera-Novoa ◽  
Jos� Luis Arredondo-Figueroa ◽  
George M Hall ◽  
Keiko Shirai

2011 ◽  
Vol 46 (12) ◽  
pp. 2460-2466 ◽  
Author(s):  
Ping Yang ◽  
Hongqiao Ke ◽  
Pengzhi Hong ◽  
Shaokui Zeng ◽  
Wenhong Cao

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3129
Author(s):  
Jun Ji ◽  
Xiangzhou Yi ◽  
Yujie Zhu ◽  
Hui Yu ◽  
Shuqi Huang ◽  
...  

The destruction of the homeostasis in the gut-brain axis can lead to cognitive impairment and memory decline. Dietary intervention with bioactive peptides from aquatic products is an innovative strategy to prevent cognitive deficits. The present study aimed to determine the neuroprotective effect of tilapia head protein hydrolysate (THPH) on scopolamine-induced cognitive impairment in mice, and to further explore its mechanism through the microbiota–gut-brain axis. The results showed that THPH administration significantly improved the cognitive behavior of mice, and normalized the cholinergic system and oxidative stress system of the mice brain. The histopathological observation showed that THPH administration significantly reduced the pathological damage of hippocampal neurons, increased the number of mature neurons marked by NeuN and delayed the activation of astrocytes in the hippocampus of mice. In addition, THPH administration maintained the stability of cholinergic system, alleviated oxidative stress and further improved the cognitive impairment by reshaping the gut microbiota structure of scopolamine-induced mice and alleviating the disorder of lipid metabolism and amino acid metabolism in serum. In conclusion, our research shows that THPH supplementation is a nutritional strategy to alleviate cognitive impairment through the gut-brain axis.


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