Enhancement of anti-complementary and radical scavenging activities in the submerged culture of Cordyceps sinensis by addition of citrus peel

2010 ◽  
Vol 101 (15) ◽  
pp. 6028-6034 ◽  
Author(s):  
Jang Won Choi ◽  
Kyung Soo Ra ◽  
Seong Yeong Kim ◽  
Taek Joon Yoon ◽  
Kwang-Won Yu ◽  
...  
Keyword(s):  
Submerged Culture ◽  
Radical Scavenging ◽  
Cordyceps Sinensis ◽  
Citrus Peel

Radical-Scavenging Activities of Cactus Cladodes (O puntia Humifusa  Raf.) in a Submerged Culture

2014 ◽  
Vol 38 (5) ◽  
pp. 491-497 ◽  
Author(s):  
Hyeon-Son Choi ◽  
Jae Hwan Kim ◽  
Yooheon Park ◽  
Kyung Soo Ra ◽  
Jee-Young Imm ◽  
...  
Keyword(s):  
Submerged Culture ◽  
Radical Scavenging

scholarly journals Amauroderma rugosum(Blume & T. Nees) Torrend: Nutritional Composition and Antioxidant and Potential Anti-Inflammatory Properties

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Pui-Mun Chan ◽  
Gowri Kanagasabapathy ◽  
Yee-Shin Tan ◽  
Vikineswary Sabaratnam ◽  
Umah Rani Kuppusamy
Keyword(s):  
Submerged Culture ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Indigenous Communities ◽  
Antioxidant Potential ◽  
Raw264.7 Cells ◽  
Total Phenolic ◽  
No Production ◽  
Freeze Dried ◽  
Anti Inflammatory

Amauroderma rugosumis a wild mushroom that is worn as a necklace by the indigenous communities in Malaysia to prevent fits and incessant crying by babies. The aim of this study was to investigate the nutritive composition and antioxidant potential and anti-inflammatory effects ofA. rugosumextracts on LPS-stimulated RAW264.7 cells. Nutritional analysis of freeze-dried mycelia ofA. rugosum(KUM 61131) from submerged culture indicated a predominant presence of carbohydrates, proteins, dietary fibre, phosphorus, potassium, and sodium. The ethanol crude extract (EE), its hexane (HF), ethyl acetate (EAF), and aqueous (AF) fractions of mycelia ofA. rugosumgrown in submerged culture were evaluated for antioxidant potential and anti-inflammatory effects. EAF exhibited the highest total phenolic content and the strongest antioxidant activity based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays. HF showed dose-dependent inhibition of NO production in LPS-stimulated RAW264.7 cells and NO radical scavenging activity. Gas chromatographic analysis of HF revealed the presence of ethyl linoleate and ergosterol, compounds with known anti-inflammatory properties. In conclusion, the nutritive compositions and significant antioxidant potential and anti-inflammatory effects of mycelia extracts ofA. rugosumhave the potential to serve as a therapeutic agent or adjuvant in the management of inflammatory disorders.

Production of mycelia and exo-biopolymer from molasses by Cordyceps sinensis 16 in submerged culture

2007 ◽  
Vol 98 (1) ◽  
pp. 165-168 ◽  
Author(s):  
S CHA ◽  
J LIM ◽  
C YOON ◽  
J KOH ◽  
H CHANG ◽  
...  
Keyword(s):  
Submerged Culture ◽  
Cordyceps Sinensis

scholarly journals Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Sapan Kumar Sharma ◽  
Nandini Gautam ◽  
Narender Singh Atri
Keyword(s):  
Submerged Culture ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Monosaccharide Composition ◽  
Culture Conditions ◽  
Scavenging Activity ◽  
Orthogonal Experiments ◽  
Nitrogen Ratio

Under present experiments, EPS and IPS production, monosaccharide composition, and antioxidant activities ofC. graciliswere studied for the first time under submerged culture conditions. Effect of different factors on polysaccharides production was studied by orthogonal experiments using one-factor-at-a-time method. Incubation of culture in the medium with capacity 200 mL (675.12 ± 5.01 and 385.20 ± 5.01 mg/L), rotation speed 150 rpm (324.62 ± 3.32 and 254.62 ± 4.62 mg/L), 6-day culture incubation time (445.24 ± 1.11, 216.60 ± 1.71 mg/L), pH 6.0 (374.81 ± 2.52 and 219.45 ± 2.59 mg/L), and temperature 23°C (405.24 ± 1.11 and 215.60 ± 1.71 mg/L) produced higher EPS and IPS, respectively. Maximum EPS and IPS production was observed in the medium supplemented with glucose as a carbon source (464.82 ± 2.12 and 264.42 ± 2.62 mg/L) and yeast extract as a nitrogen source (465.21 ± 3.11 and 245.17 ± 3.24 mg/L), respectively. Carbon to nitrogen ratio for maximum EPS and IPS production was observed as 10 : 1 (395.29 ± 2.15 and 235.62 ± 1.40 mg/L), respectively. Glucose was found to be the major monosaccharide (62.15 ± 7.33%). Both EPS and IPS ofC. gracilisshowed significant DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power, and iron chelating activity.

scholarly journals Improving Training Condition Assessment in Endurance Cyclists: Effects ofGanoderma lucidumandOphiocordyceps sinensisDietary Supplementation

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Paola Rossi ◽  
Daniela Buonocore ◽  
Elisa Altobelli ◽  
Federico Brandalise ◽  
Valentina Cesaroni ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Physical Exertion ◽  
Good Health ◽  
Cordyceps Sinensis ◽  
Market Demand ◽  
Before And After ◽  
Cortisol Ratio

The main reasons for taking daily dietary supplements are to maintain good health, to improve homeostasis, and to create conditions for reducing the risk of disease. Due to growing market demand, the search for effective, nontoxic, natural compounds with antioxidant and ergogenic properties has increasingly become a matter of interest. This paper describes how a specific combination of fungal supplements can help improve the performance of endurance athletes. We report the effects of a brief 3-month trial of two fungal supplements,Ganoderma lucidumandCordyceps sinensis(3 capsules ofO. sinensisand 2 capsules ofG. lucidumper day), in 7 healthy male volunteers, aged between 30 and 40 years, who are all amateur cyclists that participate in “Gran Fondo” cycling races. This trial investigated the effects of fungal supplements on the level of physical fitness of the athletes by monitoring and comparing the following biomarkers just before and after physical exertion: the testosterone/cortisol ratio in the saliva and oxidative stress (DPPH free radical scavenging activity). A decrease of more than 30% in the testosterone/cortisol ratio after race compared to before race was considered as a risk factor for nonfunctional overreaching (NFO) or the overtraining syndrome (OTS). The results show that, after 3 months of supplementation, the testosterone/cortisol ratio changed in a statistically significant manner, thereby protecting the athletes from NFO and OTS. Antioxidant activity was measured by quantifying the scavenging ability of the human serum on the synthetic free radical DPPH. After 3 months of fungal supplementation, the data demonstrate an increased scavenger capacity of free radicals in the athletes’ serum after the race, thereby protecting the athletes from oxidative stress.

scholarly journals Antioxidant and Immunostimulatory Activities of a Submerged Culture of Cordyceps sinensis Using Spent Coffee

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1697
Author(s):  
Sung Hee Han ◽  
Yejin Ahn ◽  
Hyun Jung Lee ◽  
Hyung Joo Suh ◽  
Kyungae Jo
Keyword(s):  
Chlorogenic Acid ◽  
Effective Means ◽  
Radical Scavenging ◽  
Cordyceps Sinensis ◽  
Microbial Fermentation ◽  
Coumaric Acid ◽  
Immunostimulatory Activity ◽  
Polyphenol Compounds ◽  
Tnf Α ◽  
Coffee Grounds

Spent coffee grounds (SCG) are inexpensive materials that have been used as a source of antioxidants and polysaccharides with immunostimulatory activity. In this study, we performed a microbial fermentation of SCG using Cordyceps sinensis and investigated the radical scavenging and immunostimulatory activity of fermented SCG. SCG fermentation using C. sinensis was performed at 25 °C for 8 d. The polyphenol content of the fermented SCG increased from 1022.4 to 1562.0 μg/mL. The glucosamine content of the mycelia also continuously increased during fermentation. The main polyphenol compounds of fermented SCG were chlorogenic acid and p-coumaric acid, which were increased by fermentation. Fermented SCG also showed significantly higher content of chlorogenic acid isomers than unfermented SCG. The fermented SCG exhibited significantly higher 2,2-diphenyl-2-picrylhydrazyl hydrate (half maximal inhibitory concentration: IC50, 0.37 mg/mL) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (IC50, 0.93 mg/mL) radical scavenging activities than those of the control (0.54 mg/mL and 1.20 mg/mL, respectively; p < 0.05). The fermented SCG stimulated macrophages and promoted the production of various immunostimulatory cytokines (IL-12, IL-6, and TNF-α) compared to control; therefore, microbial fermentation of SCG using C. sinensis is an effective means of generating antioxidant and immunostimulatory materials.

Innate immune stimulation of exo-polymers prepared from Cordyceps sinensis by submerged culture

2008 ◽  
Vol 80 (6) ◽  
pp. 1087-1093 ◽  
Author(s):  
Taek Joon Yoon ◽  
Kwang-Won Yu ◽  
Kwang-Soon Shin ◽  
Hyung Joo Suh
Keyword(s):  
Submerged Culture ◽  
Innate Immune ◽  
Cordyceps Sinensis ◽  
Immune Stimulation ◽  
Stimulation Of

Production and Preliminary Characterization of Antioxidant Polysaccharide by Submerged Culture of Culinary and Medicinal Fungi Cordyceps militaris CICC14013

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Wan Chen ◽  
Gaoqiang Liu ◽  
Huandong Yang ◽  
Zhichao Wu ◽  
Hailong Yang
Keyword(s):  
Submerged Culture ◽  
Biological Activities ◽  
Average Molecular Weight ◽  
Radical Scavenging ◽  
Deae Cellulose ◽  
Reducing Power ◽  
Cordyceps Militaris ◽  
Dpph Radical ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Abstract Polysaccharides from Cordyceps militaris show multiple biological activities, such as antioxidation, antitumor, antivirus and immunomodulating properties, etc. The nutritional requirements for antioxidant polysaccharide bio-synthesis by submerged culture of C. militaris were evaluated on the basis of assay for DPPH radical scavenging ability and reducing power in this work. The results showed the best antioxidant activity of polysaccharide could be achieved when the media consisted of mannose, peptone, MgSO4, Na2HPO4, and KH2PO4. Furthermore, the main fraction of antioxidant polysaccharide, CMP-2, was preliminary isolated from the fermentation broth of C. militaris by ethanol precipitation, filtration, and DEAE cellulose-52 chromatography. CMP-2 was composed of mannose, rhamnose, glucose and galactose in a ratio of 2527:164:172:100, and had an average molecular weight (Mw) of approximately 8175 Da. The results suggested antioxidant polysaccharide could be produced by submerged culture of C. militaris and the medium composition significantly affected its DPPH radical scavenging ability and reducing power.

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