Anaerobic fermentation of biogas liquid pretreated maize straw by rumen microorganisms in vitro

2014 ◽  
Vol 153 ◽  
pp. 8-14 ◽  
Author(s):  
Wenyao Jin ◽  
Xiaochen Xu ◽  
Yang Gao ◽  
Fenglin Yang ◽  
Gang Wang
Keyword(s):  
Anaerobic Fermentation ◽  
Rumen Microorganisms ◽  
Maize Straw

scholarly journals Isolation and Identification of a Rumen Lactobacillus Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation

2021 ◽  
Vol 9 (11) ◽  
pp. 2200
Author(s):  
Wei-Kang Wang ◽  
Wen-Juan Li ◽  
Qi-Chao Wu ◽  
Yan-Lu Wang ◽  
Sheng-Li Li ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Cottonseed Meal ◽  
Anaerobic Fermentation ◽  
Essential Amino Acids ◽  
Neutral Detergent Fiber ◽  
Isolation And Identification ◽  
Rumen Microorganisms ◽  
Acid Yield ◽  
Degrading Bacteria

Cottonseed meal (CSM) is an important protein feed source for dairy cows. Its inclusion in ruminant diets is limited due to the presence of the highly toxic gossypol though rumen microorganisms are believed to be capable of gossypol degrading and transforming. The objective of the present study was to isolate the gossypol-degrading bacteria from the rumen contents and to assess its potential for gossypol degradation in vitro. A strain named Lactobacillus agilis WWK129 was anaerobically isolated from dairy cows after mixed rumen microorganisms were grown on a substrate with gossypol as the sole carbon source. Furthermore, the strain was applied at 5% inoculum concentration in vitro to continuously ferment CSM at 39 °C for five days, and it presented gossypol degradability as high as 83%. Meanwhile, the CSM contents of crude protein, essential amino acids increased significantly along with the increase of lactic acid yield (p < 0.01). Compared with the original CSM, the fermented CSM contents of neutral detergent fiber and acid detergent fiber was remarkably decreased after the anaerobic fermentation (p < 0.01). In brief, the Lactobacillus strain isolated from the rumen is not only of great importance for gossypol biodegradation of CSM, but it could also be used to further explore the role of rumen microorganisms in gossypol degradation by the ruminants.

scholarly journals Effect of pH on Fermentation Characteristics and Protein Degradation by Rumen Microorganisms In Vitro

1982 ◽  
Vol 65 (8) ◽  
pp. 1457-1464 ◽  
Author(s):  
J.D. Erfle ◽  
R.J. Boila ◽  
R.M. Teather ◽  
S. Mahadevan ◽  
F.D. Sauer
Keyword(s):  
Protein Degradation ◽  
Rumen Microorganisms ◽  
Effect Of Ph

A NEW NON-PROTEIN NITROGEN SOURCE FOR RUMINANTS

1969 ◽  
Vol 49 (2) ◽  
pp. 135-141 ◽  
Author(s):  
L. P. Milligan ◽  
A. R. Robblee ◽  
J. C. Wood ◽  
W. C. Kay ◽  
S. K. Chakrabartty
Keyword(s):  
Nitrogen Source ◽  
Dietary Supplement ◽  
Nitrogen Retention ◽  
Feeding Trial ◽  
Short Term ◽  
Protein Nitrogen ◽  
Rumen Microorganisms ◽  
Production Of Ammonia

The preparation of a polymer of urea and furfural containing 23.2% nitrogen is described. This product was converted by rumen microorganisms in vitro to ammonia at a rate approximately one-seventh that of conversion of urea to ammonia. Use of the polymer as a dietary supplement in a feeding trial with lambs improved nitrogen retention over that of unsupplemented controls by 3.45 g of nitrogen retained per day, while an isonitrogenous quantity of supplemental urea improved nitrogen retention by 0.51 g of nitrogen retained per day. The blood urea pattern, throughout the day, of lambs adapted to control, urea-supplemented and urea–furfural polymer-supplemented rations indicated a slow, prolonged production of ammonia from the latter supplement and very rapid, short-term degradation of urea in vivo.

The Effect of Carbohydrates and Sulfur on B-Vitamins Synthesis, Cellulose Digestion, and Urea Utilization by Rumen Microorganisms In Vitro

1954 ◽  
Vol 13 (3) ◽  
pp. 570-580 ◽  
Author(s):  
Chas. H. Hunt ◽  
Orville G. Bentley ◽  
T. V. Hershberger ◽  
J. H. Cline
Keyword(s):  
B Vitamins ◽  
Rumen Microorganisms ◽  
Cellulose Digestion

Influence of ammonia concentration on [15N]ammonia uptake andde novosynthesis of different amino acids by mixed rumen microorganisms from the sheep rumenin vitro

1999 ◽  
Vol 1999 ◽  
pp. 212-212 ◽  
Author(s):  
C. Atasoglu ◽  
C.J. Newbold ◽  
R.J. Wallace
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
De Novo ◽  
Ammonia Concentration ◽  
Dependent Manner ◽  
Microbial Protein ◽  
Concentration Dependent Manner ◽  
Rumen Microorganisms ◽  
Ammonia Uptake

Ammonia is thought to be the main source of nitrogen for protein synthesis by the rumen microorganisms, but peptides and amino acids derived from protein degradation are also incorporated into microbial protein. Recent experiments carried out by Atasogluet al.(1998) demonstrated that preformed amino acids decrease the uptake of ammonia into microbial protein and microbial amino acids in a concentration-dependent manner. However, little is known about how rumen ammonia concentrations affect ammonia uptake into microbial protein. The present study was undertaken to determine the influence of rumen ammonia concentrations on ammonia incorporation andde novosynthesis of individual amino acids by the mixed rumen microorganismsin vitro.

scholarly journals Effect of Harvest Time and Microbial Anaerobic Fermentation at Ruminal Degradability, In Vitro Digestibility to Milk Production and Milk Quality for Whole Plant Zhang Hybrid Millet in Dairy Cows

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 749
Author(s):  
Yujia Tian ◽  
Xuewei Zhang ◽  
Shengli Li ◽  
Kai Liu ◽  
Peng Guo
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Yield ◽  
Anaerobic Fermentation ◽  
Milk Quality ◽  
In Vitro Digestibility ◽  
Harvest Time ◽  
Ruminal Degradability ◽  
Alternative Feedstock

This study assessed whether harvest time and microbial anaerobic fermentation could affect ruminal degradability and intestinal digestibility of whole Zhang hybrid millet, and estimate the effect of microbial anaerobic fermented whole Zhang hybrid millet as feedstuff on milk yield and milk quality. Protein degradation and intestinal digestion were determined using in situ nylon bag technique and three-step in vitro method, respectively. Results showed that harvest time, microbial anaerobic fermentation, or their interaction significantly affected EDDM, EDCP, and EDNDF (p < 0.05). In vitro fermentation was significantly influenced by harvest time. Early harvested samples appeared to have higher Total volatile fatty acid (TVFA) and lower acetate: propionate ratio than late harvested ones (p < 0.01). However, significant effect of harvest time and fermentation was failed to find in the estimation of rumen-undegradable protein (RUP) (p > 0.05). Microbial anaerobic fermented whole Zhang hybrid millet as feedstuff provided similar milk compositions compared with controls, and it significantly reduced SCC (p = 0.04). Milk yield was numerically higher in whole Zhang hybrid millet groups. In conclusion, harvest time and microbial anaerobic fermentation could further improve ruminal utilization of whole Zhang hybrid millet. Whole Zhang hybrid millet could be an alternative feedstock for dairy cows with acceptable safety profile and potential benefit in milk production.

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