Synergistic effect of fermentable and non-fermentable carbon sources enhances TAG accumulation in oleaginous yeast Rhodosporidium kratochvilovae HIMPA1

2015 ◽  
Vol 188 ◽  
pp. 136-144 ◽  
Author(s):  
Alok Patel ◽  
Vikas Pruthi ◽  
Rajesh P. Singh ◽  
Parul A. Pruthi
Keyword(s):  
Synergistic Effect ◽  
Oleaginous Yeast ◽  
Carbon Sources ◽  
Rhodosporidium Kratochvilovae ◽  
Tag Accumulation

scholarly journals Oil Production by the Oleaginous Yeast Lipomyces starkeyi using Diverse Carbon Sources

BioResources ◽  
2014 ◽  
Vol 9 (4) ◽  
Author(s):  
Ruling Wang ◽  
Jiancai Wang ◽  
Ronghua Xu ◽  
Zhen Fang ◽  
Aizhong Liu
Keyword(s):  
Oleaginous Yeast ◽  
Carbon Sources ◽  
Oil Production ◽  
Lipomyces Starkeyi

A comparison of the oleaginous yeast,Candida curvata, grown on different carbon sources in continuous and batch culture

Lipids ◽  
1983 ◽  
Vol 18 (9) ◽  
pp. 623-629 ◽  
Author(s):  
Christopher T. Evans ◽  
Colin Ratledge
Keyword(s):  
Batch Culture ◽  
Oleaginous Yeast ◽  
Carbon Sources

Efficient conversion of chitin-derived carbon sources into microbial lipid by the oleaginous yeast Cutaneotrichosporon oleaginosum

2020 ◽  
Vol 315 ◽  
pp. 123897
Author(s):  
Mou Tang ◽  
Yanan Wang ◽  
Wenting Zhou ◽  
Mian Yang ◽  
Yi Liu ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Carbon Sources ◽  
Microbial Lipid ◽  
Efficient Conversion

scholarly journals Draft Genome Sequence of the Oleaginous Yeast Cryptococcus curvatus ATCC 20509

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Dan Close ◽  
John Ojumu
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Genome Sequence ◽  
Oleaginous Yeast ◽  
Draft Genome ◽  
Carbon Sources ◽  
Draft Genome Sequence ◽  
Cryptococcus Curvatus ◽  
Production Potential ◽  
Fatty Acid Production

Cryptococcus curvatus ATCC 20509 is a commonly used nonmodel oleaginous yeast capable of converting a variety of carbon sources into fatty acids. Here, we present the draft genome sequence of this popular organism to provide a means for more in-depth studies of its fatty acid production potential.

scholarly journals Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 513
Author(s):  
Zhiyang Zhai ◽  
Hui Liu ◽  
John Shanklin
Keyword(s):  
Synergistic Effect ◽  
Oil Content ◽  
Ectopic Expression ◽  
Dry Weight ◽  
Oil Accumulation ◽  
Transgenic Lines ◽  
Leaf Oil ◽  
The Individual ◽  
Mutant Lines ◽  
Tag Accumulation

During the transformation of wild-type (WT) Arabidopsis thaliana, a T-DNA containing OLEOSIN-GFP (OLE1-GFP) was inserted by happenstance within the GBSS1 gene, resulting in significant reduction in amylose and increase in leaf oil content in the transgenic line (OG). The synergistic effect on oil accumulation of combining gbss1 with the expression of OLE1-GFP was confirmed by transforming an independent gbss1 mutant (GABI_914G01) with OLE1-GFP. The resulting OLE1-GFP/gbss1 transgenic lines showed higher leaf oil content than the individual OLE1-GFP/WT or single gbss1 mutant lines. Further stacking of the lipogenic factors WRINKLED1, Diacylglycerol O-Acyltransferase (DGAT1), and Cys-OLEOSIN1 (an engineered sesame OLEOSIN1) in OG significantly elevated its oil content in mature leaves to 2.3% of dry weight, which is 15 times higher than that in WT Arabidopsis. Inducible expression of the same lipogenic factors was shown to be an effective strategy for triacylglycerol (TAG) accumulation without incurring growth, development, and yield penalties.

scholarly journals Activating de novo triacylglycerol synthesis in oleaginous yeast for improved bio-diesel quality

2018 ◽  
Vol 4 (2) ◽  
pp. 16-24
Author(s):  
Farha Deeba ◽  
Ruchir Priyadarshi ◽  
Vikas Pruthi ◽  
Yuvraj Singh Negi
Keyword(s):  
Fatty Acid ◽  
Sodium Bicarbonate ◽  
Acid Content ◽  
Oleaginous Yeast ◽  
De Novo ◽  
Fatty Acid Content ◽  
Biodiesel Production ◽  
Nitrogen Base ◽  
Triacylglycerol Synthesis ◽  
Tag Accumulation

The economic production of yeast biofuels requires several strategies, such as multiomics techniques to gain insights into lipid biosynthesis pathway. Hence, metabolomic studies for amelioration of triacylglycerol (TAG) accumulation in oleaginous yeast strain to reveal potential targets in fatty acid synthesis pathway should be explored. The aim of this research experiment was to boost TAG agglomeration in novel isolated oleaginous yeast Cryptococcus psychrotolerans IITRFD using sodium bicarbonate as supplement for biodiesel production. Enhanced biomass productivity (83.5 ± 0.32 mg/L/h) and lipid productivity (56.8 ± 0.34 mg/L/h) have been estimated as compared to yeast nitrogen base media (YNB) used as control. Also, the bicarbonate supplementation (25 mM) leads to decrease in phospholipids (23.6 %) while increase in TAG amount (75.4 %) signifying that it redirects the phospholipids synthesis pathway in the direction of de novo TAG pathway. The fatty acid profile obtained revealed rise in monounsaturated fatty acid content and decrease in polyunsaturated fatty acid content demonstrating better oxidative stability (19 h) and cold flow behaviour (- 12 °C) of biodiesel produced. This novel strategy of utilizing bicarbonate as a triggering system may possibly revamp the commercial use of C. psychrotolerans IITRFD for high quality biodiesel production. This is the first study to augment TAG accumulation in this oleaginous yeast using sodium bicarbonate with improved fuel properties.

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