Dual-cyclical nucleic acid strand-displacement polymerization based signal amplification system for highly sensitive determination of p53 gene

Herein, an adenosine triphosphate (ATP)-fueled nucleic acid signal amplification strategy based on toehold-mediated strand displacement (TMSD) and fluorescence resonance energy transfer (FRET) was proposed for highly sensitive detection of microRNA-21.

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Fluoroimmunoassay for the Determination of Diethylstilbestrol Residue in Chicken Muscle Tissue

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.726-731.1322 ◽

2013 ◽

Vol 726-731 ◽

pp. 1322-1325

Author(s):

Wen Dong ◽

Wei Li Xue ◽

Xin Yi Wang ◽

Ling Yun Du ◽

Shu Hao Wang

Keyword(s):

Quantum Dots ◽

Signal Amplification ◽

Limit Of Detection ◽

Fluorescence Immunoassay ◽

Buffer Solution ◽

Selective Method ◽

Chicken Muscle ◽

Amplification System ◽

Signal Amplification System

A sensitive and selective method for the determination of diethylstilbestrol (DES) by fluorescence immunoassay (FIA) was established on the basis of competitive immunoreaction. The complex of avidin conjugated by quantum dots (QDs-avidin) was regarded as a probe in this system and the avidin-biotin system as the signal amplification system. After optimizing the conditions of immunoreaction such as the time and temperature of incubating, the pH of the buffer solution, the concentration of the reagent and so on, the linear range and the limit of detection of DES were 0.01-2000 ng·mL-1 and 4.5 pg·mL-1, respectively. The method was applied to determine DES in flesh of chicken, with the percent recoveries in the range of 81.0%-109.8%.

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Highly sensitive colorimetric immunoassay for Escherichia coli O157:H7 based on probe of pseudo enzyme and dual signal amplification

Analytical Methods ◽

10.1039/c8ay01410h ◽

2018 ◽

Vol 10 (35) ◽

pp. 4301-4309 ◽

Cited By ~ 3

Author(s):

Haoran Huang ◽

Zunyi Wu ◽

Jianfeng Huang ◽

Guangying Zhao ◽

Wenchao Dou

Keyword(s):

Signal Amplification ◽

Quantitative Detection ◽

Escherichia Coli O157 ◽

E Coli ◽

Reduced Graphene ◽

Highly Sensitive ◽

Amplification System ◽

Colorimetric Immunoassay ◽

Dual Signal Amplification ◽

Signal Amplification System

A sensitive colorimetric immunoassay was established for quantitative detection of E. coli O157:H7. Au@Pt with peroxidase-like activity was combined with flaky reduced graphene oxide to form a chromogenic probe of pseudo enzyme. HRP was modified on rGO-NR–Au@Pt and a dual signal amplification system was realized.

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A highly sensitive electrochemical aptasensor for detection of microcystin-LR based on a dual signal amplification strategy

The Analyst ◽

10.1039/c8an01971a ◽

2019 ◽

Vol 144 (5) ◽

pp. 1671-1678 ◽

Cited By ~ 14

Author(s):

Xiaoqiang Liu ◽

Yunfei Tang ◽

Peipei Liu ◽

Liwei Yang ◽

Lele Li ◽

...

Keyword(s):

Horseradish Peroxidase ◽

Signal Amplification ◽

Electrochemical Aptasensor ◽

Ternary Composite ◽

Highly Sensitive ◽

Amplification System ◽

Amplification Strategy ◽

Dual Signal Amplification ◽

Signal Amplification System

In this work, a sensitive and selective electrochemical aptasensor for detection of microcystin-LR was developed based on a dual signal amplification system consisted of a novel ternary composite and horseradish peroxidase.

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