Bioelectricity Production from Sweat-Activated Germination of Bacterial Endospores

2021 ◽  
pp. 113293
Author(s):  
Jihyun Ryu ◽  
Seokheun Choi
Keyword(s):  
Bacterial Endospores

scholarly journals Field Evaluation of Biological Control of Fire Blight in the Eastern United States

Plant Disease ◽  
2009 ◽  
Vol 93 (4) ◽  
pp. 386-394 ◽  
Author(s):  
George W. Sundin ◽  
Nicole A. Werner ◽  
Keith S. Yoder ◽  
Herb S. Aldwinckle
Keyword(s):  
United States ◽  
New York ◽  
Biological Control ◽  
Fire Blight ◽  
Field Evaluation ◽  
Pantoea Agglomerans ◽  
Eastern United States ◽  
Bacterial Antagonists ◽  
Bacterial Endospores ◽  
Control Options

The bacterial antagonists Pseudomonas fluorescens A506, Pantoea agglomerans C9-1, and Pantoea agglomerans E325 and preparations of Bacillus subtilis QST 713 containing bacterial endospores and lipopeptide metabolites were evaluated for efficacy in controlling fire blight in Michigan, New York, and Virginia. When examined individually, the biological control materials were not consistently effective in reducing blossom infection. The average reduction in blossom infection observed in experiments conducted between 2001 and 2007 was variable and ranged from 9.1 to 36.1%, while control with streptomycin was consistent and ranged from 59.0 to 67.3%. Incidence of blossom colonization by the bacterial antagonists was inconsistent, and <60% of stigmata had the antagonists present in 12 of 25 experiments. Consistent control of blossom infection was observed when the biological control materials were integrated into programs with streptomycin, resulting in a reduction of the number of streptomycin applications needed to yield similar levels of control. Our results indicate that the prospects for biological control of fire blight in the eastern United States are currently not high due to the variability in efficacy of existing biological control options.

Effects of Cold Atmospheric Pressure Plasma Jeton the Viability of Bacillus subtilis Endospores

2013 ◽  
Vol 647 ◽  
pp. 524-531
Author(s):  
Vinita Sharma ◽  
Katsuhiko Hosoi ◽  
Tamio Mori ◽  
Shin-ichi Kuroda
Keyword(s):  
Bacillus Subtilis ◽  
Atmospheric Pressure ◽  
Dipicolinic Acid ◽  
Atmospheric Pressure Plasma ◽  
Dilution Method ◽  
Pressure Plasma ◽  
Agar Disc ◽  
Bacterial Endospores ◽  
Cold Atmospheric Pressure Plasma ◽  
Ar Gas

In this study, we conducted experiments to investigate the effectiveness of a non-equilibrium Ar-N2 plasma jet generated by a Cold Atmospheric Pressure Plasma Torch (CAPPLAT) at a sinusoidal voltage of 20 kV, frequency of 30 kHz with 10 slm of Ar gas and 100 sccm of N2 gas. Highly environmental stress resistant bacterial endospores of Bacillus subtilis, dried on an agar disc were exposed to the plasma discharge from the CAPPLAT for different durations. The viability of spores after plasma exposure was checked by counting CFUs by serial dilution method. We also measured the amount of released DPA (dipicolinic acid, pyridine-2, 6-dicarboxylic acid), which is exclusively found in endospore protoplast (cortex), to confirm the disintegration of the cortex. We could successfully inactivate a population of Bacillus endospores of about 1.0 × 107 to 4.0 × 107 spores/ml.

Bacterial endospores the ultimate survivors

2002 ◽  
Vol 12 (2-3) ◽  
pp. 217-223 ◽  
Author(s):  
Abdelmadjid Atrih ◽  
Simon J Foster
Keyword(s):  
Bacterial Endospores

scholarly journals Paper-based inoculum of Bacillus megaterium and its practical application for simple culture preparation

2019 ◽  
Vol 72 ◽  
pp. 195-204
Author(s):  
Mana Kanjanamaneesathian ◽  
Wasunan Nimanong
Keyword(s):  
Seed Germination ◽  
Bacillus Megaterium ◽  
Large Scale ◽  
Bacterial Culture ◽  
Culture Technique ◽  
Damping Off ◽  
Bacterial Endospores ◽  
Yardlong Bean ◽  
Bacterium Bacillus ◽  
Filter Papers

The bacterium Bacillus megaterium can be used to biologically control sheath blight and grain discoloration in rice. Large-scale inoculations using liquid cultures are cumbersome so the efficacy of an alternative, paper-based system was examined. Bacterial endospores were embedded on filter papers and multiplied using a simple culture technique. The resulting suspension was used to pre-soak yardlong bean and cucumber seeds before sowing to assess its effect on seed germination and weight. The efficacy of the bacterium in protecting cucumber seeds from pre-emergent damping-off was also examined. The population of bacteria embedded in paper declined initially but remained stable for 150 days at room temperature. Bacterial culture reduced seed germination of cucumber and seedling weight of yardlong beans. Dilution with water either increased or reduced germination of cucumber seeds depending on concentration. A 1:10 v/v dilution increased cucumber-seed germination in a pre-emergent damping-off pot test but all seedlings later died, irrespective of treatment. Paper-based inoculum has the potential to replace liquid inoculum but further work is required to optimise the concentrations of the bacterial culture to achieve disease control without adversely affecting the host plant.

Effect of dietary supplementation with powder derived from Moringa oleifera and Azadirachta indica leaves on growth and microbial load of edible crickets

2021 ◽  
pp. 1-14
Author(s):  
J. Ng’ang’a ◽  
S. Imathiu ◽  
F. Fombong ◽  
J. Vanden Broeck ◽  
J. Kinyuru
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Azadirachta Indica ◽  
Moringa Oleifera ◽  
Antibacterial Properties ◽  
Acheta Domesticus ◽  
The Body ◽  
Microbial Load ◽  
Field Cricket ◽  
Bacterial Endospores

Cricket farming has shown its potential to address food and nutrition insecurity in parts of the world. However, one of the remaining challenges limiting mass production of edible insects is the affordability of feeds. One possible way to address this issue would be to explore the use of traditional plants such as Moringa oleifera leaf (MOL) and Azadirachta indica leaf (AIL) that are locally available, are protein-rich and possesses some antibacterial properties in insect feed formulation. In this regard, a study was conducted to evaluate the effect of supplementation of MOL (5 or 10%) and AIL (5 or 10%) powder in the commercially used starter chicken feed on growth and microbial load of house cricket (Acheta domesticus) and field cricket (Gryllus bimaculatus). After feeding for four weeks, the supplemented feeds with 10% MOL or 10% AIL in A. domesticus and G. bimaculatus significantly decreased (P<0.05) the body weights. Also, supplementation with MOL (5 or 10%) and AIL (5 or 10%) increased the mortality in both cricket species. Although high counts of total aerobic, Enterobacteriaceae, lactic acid bacteria, bacterial endospores, yeasts and moulds were observed, significantly lower counts (P<0.05) of lactic acid bacteria and bacterial endospores were observed in treatments containing MOL (5 or 10%) and 10% AIL in both cricket species. After processing (toasting and boiling), significant reductions (P<0.001) of all vegetative microbial cells were observed but bacterial endospores were not completely eliminated. Thus, during production and processing of insects and insect-derived products, bacterial endospores would require special attention. Salmonella and Escherichia coli were not detected in processed crickets’ samples. In conclusion, supplementation with 5% MOL or 5% AIL can produce crickets with similar body weight as control feed. Mortality increased significantly when feeds were supplemented with either MOL or AIL powder. Supplementation with MOL and AIL powders significantly reduced the cell numbers for some bacterial groups in both cricket species.

scholarly journals Freezing Tolerance of Thermophilic Bacterial Endospores in Marine Sediments

2019 ◽  
Vol 10 ◽  
Author(s):  
Margaret A. Cramm ◽  
Anirban Chakraborty ◽  
Carmen Li ◽  
S. Emil Ruff ◽  
Bo Barker Jørgensen ◽  
...  
Keyword(s):  
Freezing Tolerance ◽  
Marine Sediments ◽  
Bacterial Endospores
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